Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0277
(621 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g26320.1 68415.m03158 MADS-box protein (AGL33) contains Pfam ... 33 0.20
At2g33435.1 68415.m04098 RNA recognition motif (RRM)-containing ... 31 0.62
At5g20640.1 68418.m02452 hypothetical protein contains Pfam prof... 30 1.1
At2g43945.1 68415.m05462 expressed protein 30 1.4
At2g07696.1 68415.m00947 ribosomal protein S7 family protein con... 30 1.4
At3g48640.1 68416.m05310 expressed protein 29 1.9
At3g20250.1 68416.m02565 pumilio/Puf RNA-binding domain-containi... 29 1.9
At5g65490.1 68418.m08236 expressed protein similar to unknown pr... 29 3.3
At4g28360.1 68417.m04059 ribosomal protein L22 family protein 29 3.3
At3g10740.1 68416.m01293 glycosyl hydrolase family protein 51 si... 29 3.3
At1g10320.1 68414.m01162 U2 snRNP auxiliary factor-related simil... 29 3.3
At5g65460.1 68418.m08232 kinesin motor protein-related contains ... 28 4.3
At5g06970.1 68418.m00789 expressed protein 28 5.7
At4g14210.2 68417.m02193 phytoene dehydrogenase, chloroplast / p... 27 7.6
At4g14210.1 68417.m02192 phytoene dehydrogenase, chloroplast / p... 27 7.6
At3g20020.1 68416.m02533 protein arginine N-methyltransferase fa... 27 7.6
>At2g26320.1 68415.m03158 MADS-box protein (AGL33) contains Pfam
profile PF00319: SRF-type transcription factor
(DNA-binding and dimerisation domain)
Length = 109
Score = 32.7 bits (71), Expect = 0.20
Identities = 18/71 (25%), Positives = 34/71 (47%)
Frame = +1
Query: 100 NSSTRTLSKLQIRNYAKATSFPDYYQNPIFRKEDQVKLIADSDLKNRATVPVKPPTVSET 279
N + L +I + + +S + +F+K ++V L+ DSD+ P + PTV T
Sbjct: 16 NMGRKKLKLKRIESLKERSSKFSKRKKGLFKKAEEVALLCDSDIMLIVVSPTEKPTVFNT 75
Query: 280 SSVYFDPLVNK 312
S F ++ +
Sbjct: 76 RSRSFHTILER 86
>At2g33435.1 68415.m04098 RNA recognition motif (RRM)-containing
protein contains InterPro entry IPR000504: RNA-binding
region RNP-1 (RNA recognition motif) (RRM)
Length = 979
Score = 31.1 bits (67), Expect = 0.62
Identities = 35/132 (26%), Positives = 58/132 (43%), Gaps = 11/132 (8%)
Frame = +1
Query: 76 RRLKNSSFNSSTRTLSKLQIRNYAKATSFPDYYQNPIFRKEDQVKLIADSDLKNRATV-- 249
R+ K S N T+ Q+ Y DY+++ R+E+ V+ DS K + +
Sbjct: 39 RKRKESLENVKEETVGAAQLLGYDLVEKASDYHESKNLRREEHVE---DSSRKEKEAISR 95
Query: 250 --------PVKPPTVSETSSVYFDPLVNKVIN-HVMEKGNKRLARQLVEKAFENIKRKQI 402
P+K V + + LV KV++ H EKG R + E+ + RK+
Sbjct: 96 CREEKIEKPMKEDPVG-AAQLPDKNLVEKVLDCHESEKGYDRSEKLSHEELVMDSSRKKE 154
Query: 403 ERYHLASTPEEK 438
E ++S+ EEK
Sbjct: 155 EA--ISSSREEK 164
>At5g20640.1 68418.m02452 hypothetical protein contains Pfam profile
PF04525: Protein of unknown function (DUF567)
Length = 215
Score = 30.3 bits (65), Expect = 1.1
Identities = 20/76 (26%), Positives = 36/76 (47%), Gaps = 3/76 (3%)
Frame = +1
Query: 211 LIADSDLKNRATVPVKPPTVSETSSVYFD---PLVNKVINHVMEKGNKRLARQLVEKAFE 381
L+ +S +K V KPP +S T + Y + + N +M+ K +A+ +EK E
Sbjct: 119 LVQNSSIK--ILVHGKPPKISSTRNNYVEIKGSFAERACN-IMDSDGKAIAKVRIEKEME 175
Query: 382 NIKRKQIERYHLASTP 429
+ + + YH+ P
Sbjct: 176 EMVGNKKDLYHVIVKP 191
>At2g43945.1 68415.m05462 expressed protein
Length = 289
Score = 29.9 bits (64), Expect = 1.4
Identities = 20/78 (25%), Positives = 39/78 (50%), Gaps = 7/78 (8%)
Frame = +1
Query: 70 FYRRLKNSSFNSSTRTLSKLQIRNYAKATS--FPDYYQNP-----IFRKEDQVKLIADSD 228
F RRL +SS SS L + R+Y++ + P++ +N + K+D ++ + +SD
Sbjct: 41 FKRRLSSSSCCSSQSQLFSGRCRSYSRCVTMCLPEHMRNQENTEILTDKDDHIECVLESD 100
Query: 229 LKNRATVPVKPPTVSETS 282
+ +P + + E S
Sbjct: 101 EDSGLRIPTQAQAIVEGS 118
>At2g07696.1 68415.m00947 ribosomal protein S7 family protein
contains Pfam profile PF00177: Ribosomal protein S7p/S5e
Length = 148
Score = 29.9 bits (64), Expect = 1.4
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +2
Query: 530 GITYQVPGPITEKRSLFLAIKWLLE 604
G Y VPG + R LAI+W+LE
Sbjct: 70 GTIYDVPGIVARDRQQTLAIRWILE 94
>At3g48640.1 68416.m05310 expressed protein
Length = 224
Score = 29.5 bits (63), Expect = 1.9
Identities = 27/113 (23%), Positives = 51/113 (45%), Gaps = 7/113 (6%)
Frame = +1
Query: 94 SFNSSTRTLSKLQIRNYAKATSFPD---YYQNPIFRKEDQVKLIADSDLKNRATVPVKPP 264
S+N ++++ L + N K + + Y + + + QV + D N V K
Sbjct: 33 SWNEGLKSIANLYLENTTKTLNLFEKFMYTEEVVTQYVCQVNVYWDHVTTNWDHVTTKTR 92
Query: 265 TVSETSSVYFDPLVNKVIN----HVMEKGNKRLARQLVEKAFENIKRKQIERY 411
+SE +Y D + + HVM G R ++EK +++ KRK+I ++
Sbjct: 93 KLSE-KFMYTDEFATQYESVYRDHVMLLGKIRELEVVLEKKYKHAKRKRIMKF 144
>At3g20250.1 68416.m02565 pumilio/Puf RNA-binding domain-containing
protein contains Pfam profile: PF00806 Pumilio-family
RNA binding domains (aka PUM-HD, Pumilio homology
domain) (8 copies at C-terminus)
Length = 961
Score = 29.5 bits (63), Expect = 1.9
Identities = 17/48 (35%), Positives = 25/48 (52%)
Frame = +1
Query: 295 DPLVNKVINHVMEKGNKRLARQLVEKAFENIKRKQIERYHLASTPEEK 438
D N V HV+E+G RQ++EK N+ Q+ ++ AS EK
Sbjct: 820 DQYGNYVTQHVLERGKPDERRQIIEKLTGNV--VQMSQHKYASNVVEK 865
>At5g65490.1 68418.m08236 expressed protein similar to unknown
protein (dbj BAA75199.1)
Length = 643
Score = 28.7 bits (61), Expect = 3.3
Identities = 21/74 (28%), Positives = 36/74 (48%), Gaps = 1/74 (1%)
Frame = +1
Query: 340 NKRLARQLVEKAFENIKRKQIERYHLASTPEEKAK-IELNPKDILYKAVENCKPLLQLQP 516
N+ L +EK+FEN+ ++ + + S+ K E P D + V+N LL+
Sbjct: 559 NEELKNSTLEKSFENVNQQHSSKQNEESSKTRDEKDDEFTPVDADFNLVKN---LLESYS 615
Query: 517 HQEGWYHIPGAWTN 558
Q+G +PG +N
Sbjct: 616 SQQG---LPGPASN 626
>At4g28360.1 68417.m04059 ribosomal protein L22 family protein
Length = 271
Score = 28.7 bits (61), Expect = 3.3
Identities = 20/71 (28%), Positives = 33/71 (46%), Gaps = 5/71 (7%)
Frame = +1
Query: 76 RRLKNS-----SFNSSTRTLSKLQIRNYAKATSFPDYYQNPIFRKEDQVKLIADSDLKNR 240
RR+KNS +++SSTR L + Y ++ P Y P++ Q+ + L+
Sbjct: 17 RRVKNSHISTANYSSSTRNLESPFSQGYLQSLLRPSYSSRPLYHHLQQLGISTSRQLQ-A 75
Query: 241 ATVPVKPPTVS 273
+ PV P S
Sbjct: 76 SEEPVSSPLSS 86
>At3g10740.1 68416.m01293 glycosyl hydrolase family protein 51
similar to arabinoxylan arabinofuranohydrolase isoenzyme
AXAH-II from GI:13398414 [Hordeum vulgare]
Length = 678
Score = 28.7 bits (61), Expect = 3.3
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = -2
Query: 533 YHPS*WGCNCNNGLQFSTALYKMSLG 456
Y+P WG N G ++ ALY S G
Sbjct: 144 YNPGYWGMNIEEGKKYKVALYVRSTG 169
>At1g10320.1 68414.m01162 U2 snRNP auxiliary factor-related similar
to U2 small nuclear ribonucleoprotein auxiliary factor
35 kD subunit related protein 1 (sp|Q15695)
Length = 757
Score = 28.7 bits (61), Expect = 3.3
Identities = 14/36 (38%), Positives = 24/36 (66%)
Frame = +1
Query: 349 LARQLVEKAFENIKRKQIERYHLASTPEEKAKIELN 456
++R+ KA + +KRKQ+ R +A+ E+AK +LN
Sbjct: 33 MSRKEKRKAMKKLKRKQV-RKEIAAKEREEAKAKLN 67
>At5g65460.1 68418.m08232 kinesin motor protein-related contains
similarity to kinesin heavy chain
Length = 1281
Score = 28.3 bits (60), Expect = 4.3
Identities = 24/101 (23%), Positives = 45/101 (44%), Gaps = 3/101 (2%)
Frame = +1
Query: 175 QNPIFRKEDQVKLIADSDLKNRATVPVKP-PTVSETSSVYFDPLVNKVINHVMEKGNKRL 351
Q+ + E Q+ SD+ R+ P++P P +E + V K + ++K + +
Sbjct: 592 QSKVKDLESQLSKALKSDM-TRSRDPLEPQPRAAENT--LDSSAVTKKLEEELKKRDALI 648
Query: 352 AR--QLVEKAFENIKRKQIERYHLASTPEEKAKIELNPKDI 468
R + EK F+ + K + S+P KA + P D+
Sbjct: 649 ERLHEENEKLFDRLTEKSVASSTQVSSPSSKASPTVQPADV 689
>At5g06970.1 68418.m00789 expressed protein
Length = 1101
Score = 27.9 bits (59), Expect = 5.7
Identities = 13/41 (31%), Positives = 23/41 (56%)
Frame = +2
Query: 374 HSKISKGNKLNDTIWLLHQKKKPK*S*ILRTFYIKPLKIAN 496
H +S+ +KL D++WL KKP+ ++R ++ K N
Sbjct: 854 HYAVSQLSKLEDSMWLRWIAKKPREKIVIRKSMVEKSKSFN 894
>At4g14210.2 68417.m02193 phytoene dehydrogenase, chloroplast /
phytoene desaturase (PDS) identical to SP|Q07356
Phytoene dehydrogenase, chloroplast precursor (EC
1.14.99.-) (Phytoene desaturase){Arabidopsis thaliana};
high similarity to phytoene desaturase [Lycopersicon
esculentum][GI:19287]
Length = 566
Score = 27.5 bits (58), Expect = 7.6
Identities = 16/84 (19%), Positives = 38/84 (45%)
Frame = +1
Query: 304 VNKVINHVMEKGNKRLARQLVEKAFENIKRKQIERYHLASTPEEKAKIELNPKDILYKAV 483
+++ + +++ K L + ++ + + +I +YH+ TP +YK +
Sbjct: 449 ISRTDSDIIDATMKELEKLFPDEISADQSKAKILKYHVVKTPRS-----------VYKTI 497
Query: 484 ENCKPLLQLQPHQEGWYHIPGAWT 555
NC+P LQ +++ G +T
Sbjct: 498 PNCEPCRPLQRSPIEGFYLAGDYT 521
>At4g14210.1 68417.m02192 phytoene dehydrogenase, chloroplast /
phytoene desaturase (PDS) identical to SP|Q07356
Phytoene dehydrogenase, chloroplast precursor (EC
1.14.99.-) (Phytoene desaturase){Arabidopsis thaliana};
high similarity to phytoene desaturase [Lycopersicon
esculentum][GI:19287]
Length = 566
Score = 27.5 bits (58), Expect = 7.6
Identities = 16/84 (19%), Positives = 38/84 (45%)
Frame = +1
Query: 304 VNKVINHVMEKGNKRLARQLVEKAFENIKRKQIERYHLASTPEEKAKIELNPKDILYKAV 483
+++ + +++ K L + ++ + + +I +YH+ TP +YK +
Sbjct: 449 ISRTDSDIIDATMKELEKLFPDEISADQSKAKILKYHVVKTPRS-----------VYKTI 497
Query: 484 ENCKPLLQLQPHQEGWYHIPGAWT 555
NC+P LQ +++ G +T
Sbjct: 498 PNCEPCRPLQRSPIEGFYLAGDYT 521
>At3g20020.1 68416.m02533 protein arginine N-methyltransferase
family protein similar to SP|Q96LA8 Protein arginine
N-methyltransferase 6 (EC 2.1.1.-) {Homo sapiens}
Length = 435
Score = 27.5 bits (58), Expect = 7.6
Identities = 20/63 (31%), Positives = 31/63 (49%)
Frame = +1
Query: 199 DQVKLIADSDLKNRATVPVKPPTVSETSSVYFDPLVNKVINHVMEKGNKRLARQLVEKAF 378
+Q K SD +T P PPT + + VYF ++ + V+E G+ L++ K F
Sbjct: 356 NQKKRTNPSDALVLSTSPESPPTHWQQTIVYFYDPIDVEQDQVIE-GSVTLSQSKENKRF 414
Query: 379 ENI 387
NI
Sbjct: 415 MNI 417
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,783,045
Number of Sequences: 28952
Number of extensions: 264278
Number of successful extensions: 758
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 730
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 755
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1255974912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -