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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0266
         (782 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_30331| Best HMM Match : Amidase (HMM E-Value=0)                     84   1e-16
SB_16360| Best HMM Match : No HMM Matches (HMM E-Value=.)              33   0.26 
SB_50457| Best HMM Match : Amidase (HMM E-Value=2.6e-36)               31   0.80 
SB_32384| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   1.1  
SB_7476| Best HMM Match : No HMM Matches (HMM E-Value=.)               31   1.4  
SB_46608| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   1.4  
SB_22619| Best HMM Match : TSP_1 (HMM E-Value=8.5e-14)                 30   1.8  
SB_56012| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   1.8  
SB_43670| Best HMM Match : DUF827 (HMM E-Value=0.52)                   30   2.4  
SB_32301| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.2  
SB_23676| Best HMM Match : DUF904 (HMM E-Value=0.44)                   29   4.2  
SB_54251| Best HMM Match : rve (HMM E-Value=0.032)                     29   5.6  
SB_37851| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   7.4  
SB_13311| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   7.4  
SB_3948| Best HMM Match : No HMM Matches (HMM E-Value=.)               28   7.4  
SB_50358| Best HMM Match : DSL (HMM E-Value=6.2e-13)                   28   9.8  
SB_21876| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   9.8  
SB_4595| Best HMM Match : TSP_1 (HMM E-Value=2.5e-05)                  28   9.8  
SB_33855| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   9.8  
SB_10203| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   9.8  

>SB_30331| Best HMM Match : Amidase (HMM E-Value=0)
          Length = 555

 Score = 84.2 bits (199), Expect = 1e-16
 Identities = 47/129 (36%), Positives = 72/129 (55%), Gaps = 1/129 (0%)
 Frame = +3

Query: 228 ILFKSATKLADMIRKKQITSEEVVSAFIERCKEVNPYVNAVIEPRYEVALREARGIDKMI 407
           +L +    L   IR   +T+EEV+ A+I+R  EVNP VNA+   R++ AL EAR ID+++
Sbjct: 67  LLLQPVDALVGNIRDNTVTAEEVMRAYIKRILEVNPMVNAITNDRFDEALEEARRIDEIL 126

Query: 408 KSTDRTPEELE-QEYPLLGIPLTIKESIAVEGMSNDCGTVYTNRNPAKKDXXXXXXXXXX 584
            +   + E+ E    PLLG+P+T+KESI+  GM +  G V      ++ D          
Sbjct: 127 GNELNSEEKKELLAKPLLGVPITVKESISCRGMPHSSGLVERKNVISEHDSEVVENLRQN 186

Query: 585 XXXPIAVTS 611
              P+AVT+
Sbjct: 187 GAIPMAVTN 195



 Score = 39.5 bits (88), Expect = 0.003
 Identities = 16/24 (66%), Positives = 17/24 (70%)
 Frame = +1

Query: 619 QLCMNWETYNNVIGVTMNPYDPKR 690
           +LCM WET NNV G T NPYD  R
Sbjct: 198 ELCMWWETVNNVYGRTRNPYDTSR 221



 Score = 31.9 bits (69), Expect = 0.60
 Identities = 13/24 (54%), Positives = 19/24 (79%)
 Frame = +2

Query: 710 GGESALISSPPSLIGLGSDMAGSL 781
           GGE A+I++  SL G+GSD+ GS+
Sbjct: 228 GGEGAIIAAAGSLCGVGSDVGGSI 251


>SB_16360| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 379

 Score = 33.1 bits (72), Expect = 0.26
 Identities = 13/24 (54%), Positives = 16/24 (66%)
 Frame = +1

Query: 619 QLCMNWETYNNVIGVTMNPYDPKR 690
           +LCM WE+ N V G T NP+D  R
Sbjct: 40  ELCMWWESANRVYGRTCNPFDTAR 63


>SB_50457| Best HMM Match : Amidase (HMM E-Value=2.6e-36)
          Length = 391

 Score = 31.5 bits (68), Expect = 0.80
 Identities = 28/96 (29%), Positives = 48/96 (50%), Gaps = 10/96 (10%)
 Frame = +3

Query: 264 IRKKQITSEEVVSAFIERCKEVN--------PYVNAV--IEPRYEVALREARGIDKMIKS 413
           I + + + EE++S++I+R K+ N        P +NA+  I P    A+  A+ +DK+   
Sbjct: 22  ISQGKYSCEELISSYIKRIKQYNLSSIDSNKPPINAISSINPN---AIYRAQQLDKIFAK 78

Query: 414 TDRTPEELEQEYPLLGIPLTIKESIAVEGMSNDCGT 521
                E       L  IP+TIK++I + GM    G+
Sbjct: 79  NGIVGE-------LFCIPITIKDNIDIIGMETTSGS 107


>SB_32384| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 609

 Score = 31.1 bits (67), Expect = 1.1
 Identities = 24/100 (24%), Positives = 42/100 (42%), Gaps = 1/100 (1%)
 Frame = +3

Query: 156 IIVVPVSYFSSFKRKRKCPPPTNPILFKSATKLADMIRKKQITSEEVVSAFIERCKEVNP 335
           II  P    ++ K       P   +   +ATK+A +++KK     + + A  E+ KE   
Sbjct: 496 IIQTPSKKETTSKLAEPVTNPVKSVAKATATKVAKVVKKKADLVVKKLKAVAEKLKEALK 555

Query: 336 YVNAVIE-PRYEVALREARGIDKMIKSTDRTPEELEQEYP 452
             + V E P+  VA   A+ + K  K   +  +   +  P
Sbjct: 556 KADPVAEKPKPVVAKAVAKNVAKAAKDVAKKADHAVRAQP 595


>SB_7476| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 176

 Score = 30.7 bits (66), Expect = 1.4
 Identities = 17/44 (38%), Positives = 23/44 (52%)
 Frame = +2

Query: 341 KRCNRTTV*SSAKRSKRNRQNDKVNRSHARRIRTGISTIGNSAY 472
           KR  R      +KR+KRN++N +  RS+     T IST  N  Y
Sbjct: 13  KRDKRNKRNKCSKRNKRNKRNKRKKRSYGGFATTTISTKRNKLY 56


>SB_46608| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 161

 Score = 30.7 bits (66), Expect = 1.4
 Identities = 22/68 (32%), Positives = 34/68 (50%), Gaps = 2/68 (2%)
 Frame = -2

Query: 472 VSGIPNSGYSCSNSSGV--RSVDFIILSIPLASLSATSYRGSITAFT*GLTSLHRSINAD 299
           ++ + NS  + SNS      S+D +  SI     S  S+  SI A +  + +L  SINA 
Sbjct: 79  INALSNSIDALSNSIDAFSNSIDALCNSIDALCNSIDSFCDSIDALSNSIEALCNSINAL 138

Query: 298 TTSSDVIC 275
           + S D +C
Sbjct: 139 SNSIDALC 146


>SB_22619| Best HMM Match : TSP_1 (HMM E-Value=8.5e-14)
          Length = 506

 Score = 30.3 bits (65), Expect = 1.8
 Identities = 15/36 (41%), Positives = 20/36 (55%)
 Frame = +3

Query: 111 AFMRRFLRFLVSLLAIIVVPVSYFSSFKRKRKCPPP 218
           AF  R     V LL I+ V  + F S +R+R+ PPP
Sbjct: 246 AFSMRVAALAVLLLVIVPVSQAIFWSRRRRRRSPPP 281


>SB_56012| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 354

 Score = 30.3 bits (65), Expect = 1.8
 Identities = 18/68 (26%), Positives = 32/68 (47%)
 Frame = +2

Query: 92  KDSNHERVHEEVSQISRIVAGHHSGAGFIFFKLQTKTEMSPANKSHPLQISN*ASRYDKK 271
           K+ N ER+H  +  ++  VA   +  GF     Q+ ++ S  ++SH +  S   +  + K
Sbjct: 182 KNRNKERMHAAIVDVTASVAHIRAQRGFNAEDYQSPSKSSTKSRSHSIASSQNNNHTNPK 241

Query: 272 ETDNIGRG 295
              NI  G
Sbjct: 242 PGTNISPG 249


>SB_43670| Best HMM Match : DUF827 (HMM E-Value=0.52)
          Length = 253

 Score = 29.9 bits (64), Expect = 2.4
 Identities = 14/36 (38%), Positives = 22/36 (61%)
 Frame = +2

Query: 332 SLRKRCNRTTV*SSAKRSKRNRQNDKVNRSHARRIR 439
           +L+ +   T + SS K  +R  ++DK NRS  RR+R
Sbjct: 150 ALQSKTLHTEIDSSMKEYRRKIESDKNNRSELRRVR 185


>SB_32301| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 477

 Score = 29.1 bits (62), Expect = 4.2
 Identities = 18/71 (25%), Positives = 33/71 (46%)
 Frame = -2

Query: 517 PQSLLMPSTAILSLIVSGIPNSGYSCSNSSGVRSVDFIILSIPLASLSATSYRGSITAFT 338
           P S+  P TAIL L V      G++C ++ G+    + + +    +  + S  G I AF+
Sbjct: 148 PLSMGSPVTAILGLFVLWWGWLGFNCGSTFGITGEKWQLAARAAVNTLSASIGGGIFAFS 207

Query: 337 *GLTSLHRSIN 305
                 +R ++
Sbjct: 208 YSYARYNRKVD 218


>SB_23676| Best HMM Match : DUF904 (HMM E-Value=0.44)
          Length = 591

 Score = 29.1 bits (62), Expect = 4.2
 Identities = 16/69 (23%), Positives = 34/69 (49%)
 Frame = +3

Query: 282 TSEEVVSAFIERCKEVNPYVNAVIEPRYEVALREARGIDKMIKSTDRTPEELEQEYPLLG 461
           ++EE++ +  E   +    +  +   R EV   +  GID  I  T    E+LE+E  ++ 
Sbjct: 263 SAEEIIRSNEEEVAKREEKLRKLETKRTEVPENQREGIDTQIAETQSEIEQLERENEVIE 322

Query: 462 IPLTIKESI 488
             +++K+ +
Sbjct: 323 ERMSLKDRV 331


>SB_54251| Best HMM Match : rve (HMM E-Value=0.032)
          Length = 710

 Score = 28.7 bits (61), Expect = 5.6
 Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 10/72 (13%)
 Frame = +3

Query: 354 EPRYEVAL----REARGIDKMIKST-DRTPEELEQEYPLLGIPLT-----IKESIAVEGM 503
           +P Y+ AL    R+  G   +I    + TP+ L  + P +G PLT     ++ + A E +
Sbjct: 481 KPLYQTALGNTYRKPNGFPTVISQEGESTPQVLLPDLPEMGSPLTMDTEGVETTEATEPV 540

Query: 504 SNDCGTVYTNRN 539
              CGT+ T  N
Sbjct: 541 PRPCGTLETLGN 552



 Score = 28.3 bits (60), Expect = 7.4
 Identities = 21/69 (30%), Positives = 34/69 (49%), Gaps = 10/69 (14%)
 Frame = +3

Query: 354 EPRYEVAL----REARGIDKMIKST-DRTPEELEQEYPLLGIPLT-----IKESIAVEGM 503
           +P Y+ AL    R+  G   +I    + TP+ L  + P +G PLT     ++ + A E +
Sbjct: 619 KPLYQTALGNTYRKPNGFPTVISQEGESTPQVLLPDLPEMGSPLTMDTEGVETTEATEPV 678

Query: 504 SNDCGTVYT 530
              CGT+ T
Sbjct: 679 PRPCGTLET 687


>SB_37851| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 269

 Score = 28.3 bits (60), Expect = 7.4
 Identities = 17/49 (34%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
 Frame = +3

Query: 306 FIERCKEVN--PYVNAVIEPRYEVALREARGIDKMIKSTDRTPEELEQE 446
           F  R K++   P +N VI PRY     E      +IK   R  +ELE++
Sbjct: 205 FASRAKKIKNKPEINEVITPRYPPGEGEVLDDASIIKRYKRQIKELEEQ 253


>SB_13311| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 6406

 Score = 28.3 bits (60), Expect = 7.4
 Identities = 23/79 (29%), Positives = 41/79 (51%)
 Frame = +3

Query: 276  QITSEEVVSAFIERCKEVNPYVNAVIEPRYEVALREARGIDKMIKSTDRTPEELEQEYPL 455
            Q+TS+E VSA +++ KE      A+ +    +A ++A  IDK+++S +   E L+ +   
Sbjct: 2808 QVTSQEPVSAILDKVKEQKSDNEALQD---SLAQQQA-VIDKLMQSGESVLENLDDDNEK 2863

Query: 456  LGIPLTIKESIAVEGMSND 512
              +   I E  A  G + D
Sbjct: 2864 QALQDKISELEAGFGSAKD 2882


>SB_3948| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1112

 Score = 28.3 bits (60), Expect = 7.4
 Identities = 16/69 (23%), Positives = 33/69 (47%)
 Frame = +3

Query: 282 TSEEVVSAFIERCKEVNPYVNAVIEPRYEVALREARGIDKMIKSTDRTPEELEQEYPLLG 461
           ++EEV+ +  E   +    +  +   R EV   +  G+D  I  T    E+LE+E   + 
Sbjct: 785 SAEEVIRSNEEEVAKREEKLRQLETKRKEVPENQREGVDAQIAETQSEIEQLERENEAIE 844

Query: 462 IPLTIKESI 488
             +++K+ +
Sbjct: 845 ERMSLKDRV 853


>SB_50358| Best HMM Match : DSL (HMM E-Value=6.2e-13)
          Length = 557

 Score = 27.9 bits (59), Expect = 9.8
 Identities = 17/49 (34%), Positives = 23/49 (46%)
 Frame = -3

Query: 774 PAISDPSPISDGGEEIKADSPPRRASSCTFRIVRVHRYSYNIIVCFPVH 628
           P   DP+   D G+  KAD    R S  TF +  V      ++VCF V+
Sbjct: 427 PLTDDPNIAGDTGKR-KADITSDRGSLLTFVLTGVGIVLLLVVVCFVVY 474


>SB_21876| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 681

 Score = 27.9 bits (59), Expect = 9.8
 Identities = 21/77 (27%), Positives = 33/77 (42%)
 Frame = +2

Query: 188 LQTKTEMSPANKSHPLQISN*ASRYDKKETDNIGRGCVCVYRTV*RS*SLRKRCNRTTV* 367
           ++TK   S   + H  + +   + +   +  +I R C    +   RS      C   T  
Sbjct: 370 MKTKNVSSEQGRQHKPRRNGDITCFKCNQQGHIARICTAPEKR--RSEMWCSTCRSATHN 427

Query: 368 SSAKRSKRNRQNDKVNR 418
            SA R +R RQ DKVN+
Sbjct: 428 DSACRRQRGRQTDKVNQ 444


>SB_4595| Best HMM Match : TSP_1 (HMM E-Value=2.5e-05)
          Length = 143

 Score = 27.9 bits (59), Expect = 9.8
 Identities = 12/28 (42%), Positives = 21/28 (75%), Gaps = 2/28 (7%)
 Frame = +3

Query: 141 VSLLAIIVVPVSY--FSSFKRKRKCPPP 218
           +++L +++VPVS   F S +R+R+ PPP
Sbjct: 6   LAVLLLVIVPVSQAIFWSRRRRRRSPPP 33


>SB_33855| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 879

 Score = 27.9 bits (59), Expect = 9.8
 Identities = 14/47 (29%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
 Frame = +1

Query: 514 AERYTQIEIQPRKTRLLSGKPERLVQFP*L*PVHPQLCMNW-ETYNN 651
           A RY  + I+P   +LL G  ++  +F  + P H ++ + W   Y+N
Sbjct: 426 APRYN-LTIEPSSVKLLQGNTQQAPKFDDVRPTHQRIKVTWSRPYSN 471


>SB_10203| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1091

 Score = 27.9 bits (59), Expect = 9.8
 Identities = 16/69 (23%), Positives = 33/69 (47%)
 Frame = +3

Query: 282 TSEEVVSAFIERCKEVNPYVNAVIEPRYEVALREARGIDKMIKSTDRTPEELEQEYPLLG 461
           ++EE+  +  E   +    +  +   R EV   +  GID  I  T    E+LE+E  ++ 
Sbjct: 133 SAEEITRSNEEEVAKREEKLRQLETKRTEVPENQREGIDTQIAETQSEIEQLERENEVIE 192

Query: 462 IPLTIKESI 488
             +++K+ +
Sbjct: 193 ERMSLKDRV 201


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,840,262
Number of Sequences: 59808
Number of extensions: 545787
Number of successful extensions: 1580
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1399
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1575
length of database: 16,821,457
effective HSP length: 81
effective length of database: 11,977,009
effective search space used: 2143884611
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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