BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0190 (780 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces po... 117 2e-27 SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomy... 28 1.7 SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|... 27 3.0 SPAC144.15c |cog1||Golgi transport complex subunit Cog1 |Schizos... 27 3.0 SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces pom... 27 4.0 SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosacchar... 26 5.3 SPCC1259.11c |gyp2||GTPase activating protein Gyp2 |Schizosaccha... 25 9.2 >SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces pombe|chr 1|||Manual Length = 619 Score = 117 bits (282), Expect = 2e-27 Identities = 53/125 (42%), Positives = 87/125 (69%) Frame = +1 Query: 40 VQLVGKASLAETDKITLEVAKLLKDDFLQQNSYSSYDRFCPFYKTVGMLKNIITFYDMSR 219 +QLVGK++L+ETDK+TL++A ++K+DFLQQN YS YDR CP YKT M++N+I +Y ++ Sbjct: 501 IQLVGKSALSETDKVTLDIAGIIKNDFLQQNGYSDYDRCCPLYKTYHMMRNMIAYYTKAK 560 Query: 220 HAVESTAQSDNKVTWNVIRDAMGNVLYQLSSMKFKDPVKDGEPKIKADFDQLLEDMSAAF 399 AVE+ V W+ I+++ ++ Y+L+SMKF++P +GE +I ++ L + + F Sbjct: 561 SAVET-----GSVPWSKIKESTSDIFYELTSMKFENP-NEGEKEIVEHYETLHKKIEDKF 614 Query: 400 RNLED 414 L + Sbjct: 615 HTLTE 619 >SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomyces pombe|chr 2|||Manual Length = 542 Score = 27.9 bits (59), Expect = 1.7 Identities = 15/48 (31%), Positives = 26/48 (54%) Frame = -2 Query: 404 LRKAADISSKSWSKSALILGSPSFTGSLNFMEESW*STLPMASRMTFH 261 L +AA +SS WS + L+L + S + L ++ + MA+ + FH Sbjct: 153 LGQAAGVSSTDWSCAQLLLAAVSISTDLKYIPTNQHIVGVMAAVIVFH 200 >SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1184 Score = 27.1 bits (57), Expect = 3.0 Identities = 20/69 (28%), Positives = 28/69 (40%) Frame = +3 Query: 93 GRQAAQRRLPATEQLFVVRSLLPVLQDGGHAEEHHHVLRHVAARGGVHGAVRQQGHVERH 272 G A +R PAT + V+ G H + H +VAA+ G + R+Q E H Sbjct: 18 GLHAMERYKPATSESLYVKG-----HYGPHPHKEHPAA-YVAAKTGFNATSRRQSVREHH 71 Query: 273 PRRHGQRAL 299 G L Sbjct: 72 SSHEGSAQL 80 >SPAC144.15c |cog1||Golgi transport complex subunit Cog1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 701 Score = 27.1 bits (57), Expect = 3.0 Identities = 21/85 (24%), Positives = 37/85 (43%), Gaps = 1/85 (1%) Frame = +1 Query: 358 ADFDQLLEDMSAAFRNLED*TLYCL*NIIVIETLKFTEINFN-VKRKYSE*PKLILCVVA 534 A FD +L++ + L+ L I+ L + N VK +++ +L+ C Sbjct: 427 ASFDMVLDESLQVLKKLQTLHLSFTLGDIIPNYLTLADYLLNFVKTSFAQIYELV-CSFV 485 Query: 535 NIIDILSSQHRL*LRTKECNVIIEL 609 N + ++ S LRT C I+ L Sbjct: 486 NNVAVMESSSEKQLRTSRCLKIVRL 510 >SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 772 Score = 26.6 bits (56), Expect = 4.0 Identities = 14/45 (31%), Positives = 23/45 (51%) Frame = -1 Query: 405 VAESGRHIF*KLVEIGLDLRFTVFHWVLELHGGELVEHVAHGVAD 271 V ES I+ L +G+DL FHW L ++ L +++ + D Sbjct: 643 VKESLPEIYSHLELLGVDLDAISFHWFLSVYTDTLPTNISFRIFD 687 >SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosaccharomyces pombe|chr 1|||Manual Length = 988 Score = 26.2 bits (55), Expect = 5.3 Identities = 10/22 (45%), Positives = 17/22 (77%) Frame = -1 Query: 210 VVERDDVLQHAHRLVERAEAIV 145 +V+ DD LQ A++ VER E+++ Sbjct: 1 MVQLDDALQDAYKKVEREESLI 22 >SPCC1259.11c |gyp2||GTPase activating protein Gyp2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 720 Score = 25.4 bits (53), Expect = 9.2 Identities = 7/24 (29%), Positives = 15/24 (62%) Frame = -1 Query: 387 HIF*KLVEIGLDLRFTVFHWVLEL 316 H++ K++E+ +DL+ +W L Sbjct: 360 HLYQKIIELDMDLKLITINWFFSL 383 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,890,350 Number of Sequences: 5004 Number of extensions: 57521 Number of successful extensions: 144 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 143 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 377352472 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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