BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0190
(780 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g78900.1 68414.m09198 vacuolar ATP synthase catalytic subunit... 138 3e-33
At1g16820.1 68414.m02021 vacuolar ATP synthase catalytic subunit... 42 5e-04
At1g67870.1 68414.m07750 glycine-rich protein contains non-conse... 34 0.12
At4g28190.1 68417.m04041 expressed protein 32 0.49
At2g20825.1 68415.m02452 expressed protein 32 0.49
At1g48405.1 68414.m05407 hypothetical protein 31 1.1
At3g06480.1 68416.m00750 DEAD box RNA helicase, putative similar... 29 4.6
At1g16610.2 68414.m01990 arginine/serine-rich protein, putative ... 29 4.6
At1g16610.1 68414.m01989 arginine/serine-rich protein, putative ... 29 4.6
At4g02430.2 68417.m00330 pre-mRNA splicing factor, putative / SR... 28 8.0
At3g21790.1 68416.m02748 UDP-glucoronosyl/UDP-glucosyl transfera... 28 8.0
>At1g78900.1 68414.m09198 vacuolar ATP synthase catalytic subunit A
/ V-ATPase A subunit / vacuolar proton pump alpha
subunit / V-ATPase 69 kDa subunit identical to SP|O23654
Vacuolar ATP synthase catalytic subunit A (EC 3.6.3.14)
(V-ATPase A subunit) (Vacuolar proton pump alpha
subunit) (V-ATPase 69 kDa subunit) {Arabidopsis
thaliana}
Length = 623
Score = 138 bits (335), Expect = 3e-33
Identities = 66/128 (51%), Positives = 95/128 (74%), Gaps = 1/128 (0%)
Frame = +1
Query: 40 VQLVGKASLAETDKITLEVAKLLKDDFLQQNSYSSYDRFCPFYKTVGMLKNIITFYDMSR 219
VQLVGK +LAE DKITLE AKLL++D+L QN+++ YD+FCPFYK+V M++NII FY+++
Sbjct: 496 VQLVGKDALAEGDKITLETAKLLREDYLAQNAFTPYDKFCPFYKSVWMMRNIIHFYNLAN 555
Query: 220 HAVESTAQSD-NKVTWNVIRDAMGNVLYQLSSMKFKDPVKDGEPKIKADFDQLLEDMSAA 396
AVE A D K+T+ +I+ +G++ Y+L S KF+DP +GE + F +L +D++A
Sbjct: 556 QAVERAAGMDGQKITYTLIKHRLGDLFYRLVSQKFEDPA-EGEDTLVEKFKKLYDDLNAG 614
Query: 397 FRNLED*T 420
FR LED T
Sbjct: 615 FRALEDET 622
>At1g16820.1 68414.m02021 vacuolar ATP synthase catalytic
subunit-related / V-ATPase-related / vacuolar proton
pump-related similar to Vacuolar ATP synthase catalytic
subunit A (V-ATPase A subunit) (Vacuolar proton pump
alpha subunit) (V-ATPase 69 kDa subunit) (SP:O23654)
[Arabidopsis thaliana]
Length = 93
Score = 41.9 bits (94), Expect = 5e-04
Identities = 16/37 (43%), Positives = 27/37 (72%)
Frame = +1
Query: 139 SSYDRFCPFYKTVGMLKNIITFYDMSRHAVESTAQSD 249
++YD+F P YK+V M++NII FY+++ A++ A D
Sbjct: 32 TNYDKFSPSYKSVWMMRNIIHFYNLANQAIDRAAGVD 68
>At1g67870.1 68414.m07750 glycine-rich protein contains
non-consensus GG donor splice site at exon2; modeled to
est match.
Length = 279
Score = 33.9 bits (74), Expect = 0.12
Identities = 17/46 (36%), Positives = 23/46 (50%), Gaps = 6/46 (13%)
Frame = +3
Query: 174 GGHAEEHH--HVLRHVAARG----GVHGAVRQQGHVERHPRRHGQR 293
GGH +HH H ++H G G+HG Q GH +H HG +
Sbjct: 132 GGHGIQHHDIHGMQHQGGHGMQHQGMHGMQHQGGHGMQHQGGHGMQ 177
Score = 30.3 bits (65), Expect = 1.5
Identities = 17/46 (36%), Positives = 21/46 (45%), Gaps = 6/46 (13%)
Frame = +3
Query: 174 GGHAEEHH--HVLRHVAARG----GVHGAVRQQGHVERHPRRHGQR 293
GGH EH H ++H G G HG Q GH +H HG +
Sbjct: 188 GGHGMEHQGGHGMQHQDMHGMQHQGRHGMQHQGGHEMQHQGMHGMQ 233
Score = 29.5 bits (63), Expect = 2.6
Identities = 16/46 (34%), Positives = 21/46 (45%), Gaps = 6/46 (13%)
Frame = +3
Query: 174 GGHAEEHH--HVLRHVAARG----GVHGAVRQQGHVERHPRRHGQR 293
GGH +H H ++H G G HG Q GH +H HG +
Sbjct: 164 GGHGMQHQGGHGMQHQGMHGMQHQGGHGMEHQGGHGMQHQDMHGMQ 209
Score = 28.7 bits (61), Expect = 4.6
Identities = 14/40 (35%), Positives = 18/40 (45%)
Frame = +3
Query: 174 GGHAEEHHHVLRHVAARGGVHGAVRQQGHVERHPRRHGQR 293
GGH +H + H G HG Q GH +H HG +
Sbjct: 148 GGHGMQHQGM--HGMQHQGGHGMQHQGGHGMQHQGMHGMQ 185
>At4g28190.1 68417.m04041 expressed protein
Length = 237
Score = 31.9 bits (69), Expect = 0.49
Identities = 14/29 (48%), Positives = 17/29 (58%)
Frame = +2
Query: 38 SCSWSARRPSRKLTRSRSRSPSCSKTTSC 124
+C R SRK+ R +RSPSC TSC
Sbjct: 182 TCEEEEERGSRKVYRGCTRSPSCKGCTSC 210
>At2g20825.1 68415.m02452 expressed protein
Length = 228
Score = 31.9 bits (69), Expect = 0.49
Identities = 14/29 (48%), Positives = 17/29 (58%)
Frame = +2
Query: 38 SCSWSARRPSRKLTRSRSRSPSCSKTTSC 124
+C R SRK+ R +RSPSC TSC
Sbjct: 172 TCEEEEERGSRKVFRGCTRSPSCKGCTSC 200
>At1g48405.1 68414.m05407 hypothetical protein
Length = 297
Score = 30.7 bits (66), Expect = 1.1
Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 1/32 (3%)
Frame = +1
Query: 169 KTVGMLKNIITFYDMSRH-AVESTAQSDNKVT 261
KT+G+LK ++ DM + AVE TA +K+T
Sbjct: 145 KTIGLLKKLVMLLDMEKEVAVEETANLGHKLT 176
>At3g06480.1 68416.m00750 DEAD box RNA helicase, putative similar to
RNA helicase DRH1 [Arabidopsis thaliana] GI:3149952;
contains Pfam profiles PF00270: DEAD/DEAH box helicase,
PF00271: Helicase conserved C-terminal domain, PF00397:
WW domain
Length = 1088
Score = 28.7 bits (61), Expect = 4.6
Identities = 18/38 (47%), Positives = 24/38 (63%), Gaps = 1/38 (2%)
Frame = +2
Query: 2 RRSCRRKRTCPKSCSWS-ARRPSRKLTRSRSRSPSCSK 112
RRS R+ +S S+S +R SR +RSRSRSP S+
Sbjct: 966 RRSSSYSRSRSRSGSYSRSRSRSRSWSRSRSRSPRHSR 1003
>At1g16610.2 68414.m01990 arginine/serine-rich protein, putative
(SR45) similar to arginine/serine-rich protein
GI:6601502 from [Arabidopsis thaliana]
Length = 407
Score = 28.7 bits (61), Expect = 4.6
Identities = 15/28 (53%), Positives = 19/28 (67%)
Frame = +2
Query: 38 SCSWSARRPSRKLTRSRSRSPSCSKTTS 121
S S S PSR ++RSRSRS S S ++S
Sbjct: 22 SRSRSGSSPSRSISRSRSRSRSLSSSSS 49
Score = 27.9 bits (59), Expect = 8.0
Identities = 18/39 (46%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Frame = +2
Query: 14 RRKRTCPKSCSWSARRPSRKLTRSRS-RSPSCSKTTSCN 127
RR R+ S S S RR RK++RSRS + P K +S N
Sbjct: 353 RRGRSSSYSSSPSPRRIPRKISRSRSPKRPLRGKRSSSN 391
>At1g16610.1 68414.m01989 arginine/serine-rich protein, putative
(SR45) similar to arginine/serine-rich protein
GI:6601502 from [Arabidopsis thaliana]
Length = 414
Score = 28.7 bits (61), Expect = 4.6
Identities = 15/28 (53%), Positives = 19/28 (67%)
Frame = +2
Query: 38 SCSWSARRPSRKLTRSRSRSPSCSKTTS 121
S S S PSR ++RSRSRS S S ++S
Sbjct: 22 SRSRSGSSPSRSISRSRSRSRSLSSSSS 49
Score = 27.9 bits (59), Expect = 8.0
Identities = 18/39 (46%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Frame = +2
Query: 14 RRKRTCPKSCSWSARRPSRKLTRSRS-RSPSCSKTTSCN 127
RR R+ S S S RR RK++RSRS + P K +S N
Sbjct: 360 RRGRSSSYSSSPSPRRIPRKISRSRSPKRPLRGKRSSSN 398
>At4g02430.2 68417.m00330 pre-mRNA splicing factor, putative / SR1
protein, putative strong similarity to SP|O22315
Pre-mRNA splicing factor SF2 (SR1 protein) {Arabidopsis
thaliana}; cDNA NCBI_gi:15810292 supports a truncated
version while protein evidence supports a longer model.
Length = 278
Score = 27.9 bits (59), Expect = 8.0
Identities = 19/35 (54%), Positives = 21/35 (60%)
Frame = +2
Query: 5 RSCRRKRTCPKSCSWSARRPSRKLTRSRSRSPSCS 109
RS R R+ KS S R PSR +RSRSRS S S
Sbjct: 200 RSPSRGRSYSKSRS-RGRSPSRSRSRSRSRSKSRS 233
>At3g21790.1 68416.m02748 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 495
Score = 27.9 bits (59), Expect = 8.0
Identities = 15/53 (28%), Positives = 34/53 (64%)
Frame = +1
Query: 235 TAQSDNKVTWNVIRDAMGNVLYQLSSMKFKDPVKDGEPKIKADFDQLLEDMSA 393
+A S+N++ + VI A+ ++++++ +K+ EPK+++ +LLED S+
Sbjct: 58 SASSNNRLRYEVI-SAVDQPTIEMTTIEIH--MKNQEPKVRSTVAKLLEDYSS 107
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,030,560
Number of Sequences: 28952
Number of extensions: 297959
Number of successful extensions: 900
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 823
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 893
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1746037600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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