Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0185
(851 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P15104 Cluster: Glutamine synthetase; n=312; cellular o... 167 4e-40
UniRef50_Q7T2P7 Cluster: Glutamine synthetase; n=4; Coelomata|Re... 166 5e-40
UniRef50_Q42688 Cluster: Glutamine synthetase cytosolic isozyme;... 149 7e-35
UniRef50_A1L199 Cluster: Glutamine synthetase; n=24; Eukaryota|R... 146 5e-34
UniRef50_Q43127 Cluster: Glutamine synthetase, chloroplast/mitoc... 137 4e-31
UniRef50_Q874T6 Cluster: Glutamine synthetase; n=5; Fungi/Metazo... 126 7e-28
UniRef50_Q5UR44 Cluster: Putative glutamine synthetase; n=1; Aca... 119 8e-26
UniRef50_O96463 Cluster: Glutamine synthetase; n=1; Skeletonema ... 118 2e-25
UniRef50_A0DB13 Cluster: Chromosome undetermined scaffold_44, wh... 114 3e-24
UniRef50_Q4QJ42 Cluster: Glutamine synthetase, putative; n=12; E... 103 4e-21
UniRef50_UPI0000F20B38 Cluster: PREDICTED: hypothetical protein;... 101 2e-20
UniRef50_Q7M314 Cluster: Glutamate-ammonia ligase; n=1; Bos taur... 90 8e-17
UniRef50_P20805 Cluster: Glutamine synthetase 2; n=19; Frankia|R... 84 4e-15
UniRef50_Q42689 Cluster: Glutamine synthetase, chloroplast precu... 83 9e-15
UniRef50_Q6N241 Cluster: Glutamine synthetase II; n=16; Bacteria... 77 4e-13
UniRef50_A0PCY1 Cluster: Glutamine synthetase precursor; n=1; Gu... 77 4e-13
UniRef50_P04772 Cluster: Glutamine synthetase 2; n=211; Bacteria... 77 7e-13
UniRef50_P94845 Cluster: Glutamine synthetase; n=95; Epsilonprot... 41 0.046
UniRef50_UPI000065D6C6 Cluster: Rho guanine nucleotide exchange ... 37 0.56
UniRef50_A5C4G8 Cluster: Putative uncharacterized protein; n=1; ... 36 1.3
UniRef50_A5B590 Cluster: Putative uncharacterized protein; n=1; ... 36 1.7
UniRef50_Q9BIT1 Cluster: Fibroin 2; n=1; Plectreurys tristis|Rep... 34 4.0
UniRef50_Q6ZUE9 Cluster: CDNA FLJ43768 fis, clone TESTI2049422; ... 34 4.0
UniRef50_A7IAX6 Cluster: Putative uncharacterized protein precur... 34 5.2
UniRef50_UPI0000DA3C43 Cluster: PREDICTED: similar to VPS10 doma... 33 6.9
UniRef50_A4CQL3 Cluster: Putative uncharacterized protein; n=1; ... 33 6.9
UniRef50_Q8ILR9 Cluster: Putative uncharacterized protein; n=1; ... 33 6.9
UniRef50_Q5A3J6 Cluster: Potential CCR4-NOT complex associated f... 33 6.9
>UniRef50_P15104 Cluster: Glutamine synthetase; n=312; cellular
organisms|Rep: Glutamine synthetase - Homo sapiens
(Human)
Length = 373
Score = 167 bits (405), Expect = 4e-40
Identities = 76/156 (48%), Positives = 104/156 (66%)
Frame = +2
Query: 173 LTNSPNAVLSKTLLSRYNDLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPI 352
+T S ++ L+K + Y LP +K+ A YIWIDG+GE LRCK RTL+ PK ++LP
Sbjct: 1 MTTSASSHLNKGIKQVYMSLP-QGEKVQAMYIWIDGTGEGLRCKTRTLDSEPKCVEELPE 59
Query: 353 WNFDGSSTNQADGHNSDTYLVPRAIYKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQE 532
WNFDGSST Q++G NSD YLVP A+++DPFR+ + LV+C+ +KYN P E+N R +C+
Sbjct: 60 WNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTCKR 119
Query: 533 AYDKCKDDEPWFGIEQEYILLDSDLRPFRVAPGWFP 640
D + PWFG+EQEY L+ +D PF FP
Sbjct: 120 IMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFP 155
Score = 62.9 bits (146), Expect = 1e-08
Identities = 25/36 (69%), Positives = 30/36 (83%)
Frame = +1
Query: 742 RLAGTNAEVMPLQWEFQVGPSIGVHAGDDLWVARYI 849
++AGTNAEVMP QWEFQ+GP G+ GD LWVAR+I
Sbjct: 189 KIAGTNAEVMPAQWEFQIGPCEGISMGDHLWVARFI 224
Score = 57.2 bits (132), Expect = 5e-07
Identities = 22/30 (73%), Positives = 26/30 (86%)
Frame = +3
Query: 657 YYCGVGANKVFARDLVEAHYRCCLYAGVPI 746
YYCGVGA++ + RD+VEAHYR CLYAGV I
Sbjct: 161 YYCGVGADRAYGRDIVEAHYRACLYAGVKI 190
>UniRef50_Q7T2P7 Cluster: Glutamine synthetase; n=4; Coelomata|Rep:
Glutamine synthetase - Danio rerio (Zebrafish)
(Brachydanio rerio)
Length = 371
Score = 166 bits (404), Expect = 5e-40
Identities = 75/156 (48%), Positives = 106/156 (67%)
Frame = +2
Query: 173 LTNSPNAVLSKTLLSRYNDLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPI 352
+ S ++ LSK + +Y +LP D++ A YIWIDG+GE LRCK RTL+ PK+ +DLP
Sbjct: 1 MATSASSQLSKVVKQQYMELP-QGDQVQAMYIWIDGTGEGLRCKTRTLDSEPKSIEDLPE 59
Query: 353 WNFDGSSTNQADGHNSDTYLVPRAIYKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQE 532
WNFDGSST QA+G NSD YL+P A+++DPFR+ + LV+C+ KYN + E+N+R +C++
Sbjct: 60 WNFDGSSTYQAEGSNSDMYLIPAAMFRDPFRKDPNKLVLCEVVKYNRKTAETNHRHTCKK 119
Query: 533 AYDKCKDDEPWFGIEQEYILLDSDLRPFRVAPGWFP 640
+ PWFG+EQEY +L +D PF FP
Sbjct: 120 IMEMVGHQSPWFGMEQEYTILGTDGHPFGWPSNGFP 155
Score = 60.9 bits (141), Expect = 4e-08
Identities = 25/35 (71%), Positives = 28/35 (80%)
Frame = +1
Query: 745 LAGTNAEVMPLQWEFQVGPSIGVHAGDDLWVARYI 849
+ GTNAEVMP QWEFQVGP G+ GD LWVAR+I
Sbjct: 190 ICGTNAEVMPAQWEFQVGPCEGIDMGDHLWVARFI 224
Score = 58.0 bits (134), Expect = 3e-07
Identities = 23/30 (76%), Positives = 26/30 (86%)
Frame = +3
Query: 657 YYCGVGANKVFARDLVEAHYRCCLYAGVPI 746
YYCGVGA+K + RD+VEAHYR CLYAGV I
Sbjct: 161 YYCGVGADKAYGRDIVEAHYRACLYAGVMI 190
>UniRef50_Q42688 Cluster: Glutamine synthetase cytosolic isozyme;
n=6; Eukaryota|Rep: Glutamine synthetase cytosolic
isozyme - Chlamydomonas reinhardtii
Length = 382
Score = 149 bits (362), Expect = 7e-35
Identities = 73/142 (51%), Positives = 94/142 (66%), Gaps = 11/142 (7%)
Frame = +2
Query: 248 KILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAI 427
KI A Y+WI GS +R K RTL+ IP P+DLP WN+DGSST QA GH+S+ YL+PR+I
Sbjct: 37 KICAEYVWIGGSMHDVRSKSRTLSTIPTKPEDLPHWNYDGSSTGQAPGHDSEVYLIPRSI 96
Query: 428 YKDPFRRGNHILVMCDTY---KYNME-------PTESNNRISCQEAYDKCKDDEPWFGIE 577
+KDPFR G++ILVMCD Y K N + P +N R +C E +K K +EPWFGIE
Sbjct: 97 FKDPFRGGDNILVMCDCYEPPKVNPDGTLAAPKPIPTNTRFACAEVMEKAKKEEPWFGIE 156
Query: 578 QEYILLDSDLR-PFRVAPGWFP 640
QEY LL++ + P G +P
Sbjct: 157 QEYTLLNAITKWPLGWPKGGYP 178
Score = 54.4 bits (125), Expect = 3e-06
Identities = 20/35 (57%), Positives = 28/35 (80%)
Frame = +1
Query: 745 LAGTNAEVMPLQWEFQVGPSIGVHAGDDLWVARYI 849
++G NAEV+P QWE+QVGP G+ GD +W++RYI
Sbjct: 213 ISGVNAEVLPSQWEYQVGPCEGITMGDHMWMSRYI 247
Score = 41.9 bits (94), Expect = 0.020
Identities = 17/30 (56%), Positives = 18/30 (60%)
Frame = +3
Query: 657 YYCGVGANKVFARDLVEAHYRCCLYAGVPI 746
YYC GA RD+ E HYR CL AGV I
Sbjct: 184 YYCSAGAGVAIGRDVAEVHYRLCLAAGVNI 213
>UniRef50_A1L199 Cluster: Glutamine synthetase; n=24; Eukaryota|Rep:
Glutamine synthetase - Homo sapiens (Human)
Length = 258
Score = 146 bits (355), Expect = 5e-34
Identities = 63/125 (50%), Positives = 85/125 (68%)
Frame = +2
Query: 266 IWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAIYKDPFR 445
+W G+GE LRCK RTL+ PK ++LP WNFDGSST Q++G NSD YLVP A+++DPFR
Sbjct: 1 LWAGGTGEGLRCKTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFR 60
Query: 446 RGNHILVMCDTYKYNMEPTESNNRISCQEAYDKCKDDEPWFGIEQEYILLDSDLRPFRVA 625
+ + LV+C+ +KYN P E+N R +C+ D + PWFG+EQEY L+ +D PF
Sbjct: 61 KDPNKLVLCEVFKYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWP 120
Query: 626 PGWFP 640
FP
Sbjct: 121 SNGFP 125
Score = 62.9 bits (146), Expect = 1e-08
Identities = 25/36 (69%), Positives = 30/36 (83%)
Frame = +1
Query: 742 RLAGTNAEVMPLQWEFQVGPSIGVHAGDDLWVARYI 849
++AGTNAEVMP QWEFQ+GP G+ GD LWVAR+I
Sbjct: 159 KIAGTNAEVMPAQWEFQIGPCEGISMGDHLWVARFI 194
Score = 57.2 bits (132), Expect = 5e-07
Identities = 22/30 (73%), Positives = 26/30 (86%)
Frame = +3
Query: 657 YYCGVGANKVFARDLVEAHYRCCLYAGVPI 746
YYCGVGA++ + RD+VEAHYR CLYAGV I
Sbjct: 131 YYCGVGADRAYGRDIVEAHYRACLYAGVKI 160
>UniRef50_Q43127 Cluster: Glutamine synthetase,
chloroplast/mitochondrial precursor; n=594;
Viridiplantae|Rep: Glutamine synthetase,
chloroplast/mitochondrial precursor - Arabidopsis
thaliana (Mouse-ear cress)
Length = 430
Score = 137 bits (331), Expect = 4e-31
Identities = 74/173 (42%), Positives = 101/173 (58%), Gaps = 4/173 (2%)
Frame = +2
Query: 134 KIEDNPKILSGPVLT-NSPNAVLSKTLLSRYNDLPLPADKILATYIWIDGSGEHLRCKDR 310
K + N K+ VL S N+ +++ D +D+I+A YIWI GSG LR K R
Sbjct: 37 KKQSNNKVRGFRVLALQSDNSTVNRVETLLNLDTKPYSDRIIAEYIWIGGSGIDLRSKSR 96
Query: 311 TLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAIYKDPFRRGNHILVMCDTYKYN 490
T+ + P +LP WN+DGSST QA G +S+ L P+AI++DPFR GN+ILV+CDT+
Sbjct: 97 TIEKPVEDPSELPKWNYDGSSTGQAPGEDSEVILYPQAIFRDPFRGGNNILVICDTWTPA 156
Query: 491 MEPTESNNRISCQEAYD--KCKDDEPWFGIEQEYILLDSDLR-PFRVAPGWFP 640
EP +N R E + K + PWFGIEQEY LL +++ P G FP
Sbjct: 157 GEPIPTNKRAKAAEIFSNKKVSGEVPWFGIEQEYTLLQQNVKWPLGWPVGAFP 209
Score = 62.5 bits (145), Expect = 1e-08
Identities = 24/35 (68%), Positives = 30/35 (85%)
Frame = +1
Query: 745 LAGTNAEVMPLQWEFQVGPSIGVHAGDDLWVARYI 849
++GTN EVMP QWEFQVGPS+G+ AGD +W ARY+
Sbjct: 244 ISGTNGEVMPGQWEFQVGPSVGIDAGDHVWCARYL 278
Score = 53.2 bits (122), Expect = 8e-06
Identities = 19/30 (63%), Positives = 26/30 (86%)
Frame = +3
Query: 657 YYCGVGANKVFARDLVEAHYRCCLYAGVPI 746
YYCGVGA+K++ RD+ +AHY+ CLYAG+ I
Sbjct: 215 YYCGVGADKIWGRDISDAHYKACLYAGINI 244
>UniRef50_Q874T6 Cluster: Glutamine synthetase; n=5; Fungi/Metazoa
group|Rep: Glutamine synthetase - Kluyveromyces lactis
(Yeast) (Candida sphaerica)
Length = 372
Score = 126 bits (304), Expect = 7e-28
Identities = 66/150 (44%), Positives = 90/150 (60%), Gaps = 1/150 (0%)
Frame = +2
Query: 194 VLSKT-LLSRYNDLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGS 370
++ KT +L +Y +L I+A Y+WID G LR K RTLN + LP WNFDGS
Sbjct: 7 IVEKTHILQKYLELD-QRGAIIAEYVWIDSEGG-LRSKGRTLNKKVTSVDSLPEWNFDGS 64
Query: 371 STNQADGHNSDTYLVPRAIYKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEAYDKCK 550
ST QA GH+SD YL P A Y DPFRRG++I+V+ + + + P + N+R + ++ K
Sbjct: 65 STGQAPGHDSDIYLKPVAFYPDPFRRGDNIVVLAECWNNDGTPNKFNHRHEAAKLFEAHK 124
Query: 551 DDEPWFGIEQEYILLDSDLRPFRVAPGWFP 640
D E WFG+EQEY L D + + G FP
Sbjct: 125 DAEMWFGLEQEYTLFDQYDQVYGWPKGGFP 154
Score = 61.3 bits (142), Expect = 3e-08
Identities = 25/30 (83%), Positives = 27/30 (90%)
Frame = +3
Query: 657 YYCGVGANKVFARDLVEAHYRCCLYAGVPI 746
YYCGVGA KVFARD++EAHYR CLYAGV I
Sbjct: 160 YYCGVGAGKVFARDVIEAHYRACLYAGVNI 189
Score = 55.6 bits (128), Expect = 1e-06
Identities = 22/34 (64%), Positives = 26/34 (76%)
Frame = +1
Query: 745 LAGTNAEVMPLQWEFQVGPSIGVHAGDDLWVARY 846
++G NAEVMP QWEFQVGP G+ D LW+ARY
Sbjct: 189 ISGINAEVMPSQWEFQVGPCEGIAMADQLWIARY 222
>UniRef50_Q5UR44 Cluster: Putative glutamine synthetase; n=1;
Acanthamoeba polyphaga mimivirus|Rep: Putative glutamine
synthetase - Mimivirus
Length = 353
Score = 119 bits (287), Expect = 8e-26
Identities = 56/118 (47%), Positives = 82/118 (69%), Gaps = 4/118 (3%)
Frame = +2
Query: 263 YIWIDGSGEHLRCKDRTL-NFIPKAPK--DLPIWNFDGSSTNQADGHNSDTYLVPRAIYK 433
Y+WI G+GE LR K R L + I K D+P+WN+DGSSTNQA+G +S+ +L PR IY+
Sbjct: 23 YVWIGGNGE-LRSKTRVLYSSIMTGYKLSDIPVWNYDGSSTNQANGSSSEVFLYPRNIYR 81
Query: 434 DPFRRG-NHILVMCDTYKYNMEPTESNNRISCQEAYDKCKDDEPWFGIEQEYILLDSD 604
PFRR N ++V+CDTY N P E+N+R + ++K ++++PW+G+EQEY + D
Sbjct: 82 CPFRRNVNGVIVICDTYDVNGVPLETNHRHNANIIFEKYQNEKPWYGLEQEYFIFRKD 139
Score = 52.0 bits (119), Expect = 2e-05
Identities = 22/50 (44%), Positives = 32/50 (64%)
Frame = +1
Query: 700 LSKPTIDAVFMLAYRLAGTNAEVMPLQWEFQVGPSIGVHAGDDLWVARYI 849
+S ++A +++GTN EV P Q EFQ+GP G+ A D LW+AR+I
Sbjct: 169 ISDEHMEACLYAGIKISGTNLEVAPGQHEFQIGPVEGIDAADQLWIARFI 218
Score = 37.9 bits (84), Expect = 0.32
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +3
Query: 657 YYCGVGANKVFARDLVEAHYRCCLYAGVPI 746
YYC VG+ + R + + H CLYAG+ I
Sbjct: 155 YYCSVGSQNAYGRRISDEHMEACLYAGIKI 184
>UniRef50_O96463 Cluster: Glutamine synthetase; n=1; Skeletonema
costatum|Rep: Glutamine synthetase - Skeletonema
costatum (Marine centric diatom)
Length = 410
Score = 118 bits (284), Expect = 2e-25
Identities = 62/135 (45%), Positives = 83/135 (61%), Gaps = 5/135 (3%)
Frame = +2
Query: 197 LSKTLLSRYNDLPLPADKILATYIWIDGSGEHLRCKDRTLNFI-PKAPKDLPIWNFDGSS 373
L +++ R++ LP P DK+LA Y+W+D GE R K RTL +A LP WNFDGSS
Sbjct: 45 LDTSVVDRFSALPYPDDKVLAEYVWVDAKGE-CRSKTRTLPVARTEAVDKLPNWNFDGSS 103
Query: 374 TNQADGHNSDTYLVPRAIYKDPFRRGNH----ILVMCDTYKYNMEPTESNNRISCQEAYD 541
T+QA G +S+ L P I+KDPFR H LVMCDTY E +N R +A++
Sbjct: 104 TDQAPGDDSEVILRPCRIFKDPFRPRAHGLDNNLVMCDTYTPAGEAIPTNTRAIAAKAFE 163
Query: 542 KCKDDEPWFGIEQEY 586
+D+E WFG+EQE+
Sbjct: 164 GKEDEEVWFGLEQEF 178
Score = 54.8 bits (126), Expect = 3e-06
Identities = 33/99 (33%), Positives = 49/99 (49%), Gaps = 4/99 (4%)
Frame = +1
Query: 565 VWDRTGIYFTGFRSAPLSGGPRV----VSPHRKGHTTAVSVPTKSLPETLSKPTIDAVFM 732
VW FT + P + PR+ VS R G + + P S ++
Sbjct: 170 VWFGLEQEFTPLQPRP-THSPRLAQEPVSQPRSGPYYSSAGPENSFGRAVTDAMYRCCLY 228
Query: 733 LAYRLAGTNAEVMPLQWEFQVGPSIGVHAGDDLWVARYI 849
++GTN EVMP Q E+Q+GP +G+ AGD L+++RYI
Sbjct: 229 AGLEISGTNGEVMPGQQEYQIGPCVGIDAGDQLFMSRYI 267
>UniRef50_A0DB13 Cluster: Chromosome undetermined scaffold_44, whole
genome shotgun sequence; n=11; Oligohymenophorea|Rep:
Chromosome undetermined scaffold_44, whole genome
shotgun sequence - Paramecium tetraurelia
Length = 398
Score = 114 bits (274), Expect = 3e-24
Identities = 56/116 (48%), Positives = 72/116 (62%), Gaps = 1/116 (0%)
Frame = +2
Query: 251 ILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAIY 430
+LA YIWIDG+GE LR K + K +DL W +DGSST+QA S+ YL P +
Sbjct: 25 VLAEYIWIDGTGEQLRSKTKVYQTQIKRLEDLEWWTYDGSSTDQAVTRFSEIYLKPVRVV 84
Query: 431 KDPFRRGNHILVMCDTYKYNME-PTESNNRISCQEAYDKCKDDEPWFGIEQEYILL 595
KDPFR HILV+C+TY + + P N R + +K +D +PWFGIEQEY LL
Sbjct: 85 KDPFRGDPHILVLCETYLPDKKTPARYNFRWIANQIMEKARDHKPWFGIEQEYFLL 140
Score = 56.0 bits (129), Expect = 1e-06
Identities = 27/77 (35%), Positives = 40/77 (51%)
Frame = +1
Query: 619 GGPRVVSPHRKGHTTAVSVPTKSLPETLSKPTIDAVFMLAYRLAGTNAEVMPLQWEFQVG 798
G P P+ +G + L++ + A ++AG NAEV P QWEFQ+G
Sbjct: 152 GWPTGGFPYPQGRYYCSIGERNNFGRALAEAHLRACLNAGLKIAGLNAEVAPSQWEFQIG 211
Query: 799 PSIGVHAGDDLWVARYI 849
+ G+ GD +W+ARYI
Sbjct: 212 IAEGIEIGDHMWLARYI 228
>UniRef50_Q4QJ42 Cluster: Glutamine synthetase, putative; n=12;
Eukaryota|Rep: Glutamine synthetase, putative -
Leishmania major
Length = 536
Score = 103 bits (248), Expect = 4e-21
Identities = 61/176 (34%), Positives = 90/176 (51%), Gaps = 16/176 (9%)
Frame = +2
Query: 161 SGPVLTNSPNAVLSKTLLSRYNDLPLPADKILATYIWIDGSGEH--LRCKDRTLNF---- 322
S P N+ A + +S N + TYIW+ G H +R KDRT+
Sbjct: 141 SSPTTDNTATAATNSITMSSSNK-----QTVRVTYIWLSGKDSHHDIRSKDRTMYLSQEN 195
Query: 323 IPKAPKDL------PIWNFDGSSTNQADGHNSDTYLVPRAIYKDPFRRGNH----ILVMC 472
+ K PKDL P+WNFDGSST QA G +++ L P + R + ILV+
Sbjct: 196 VAKHPKDLLANGVFPVWNFDGSSTGQAKGVDTEILLKPVNAFPCCLPRTSSKIPWILVLA 255
Query: 473 DTYKYNMEPTESNNRISCQEAYDKCKDDEPWFGIEQEYILLDSDLRPFRVAPGWFP 640
+ Y + EPT N+R + +E +++C ++ PWFG+EQEY ++ D RP+ FP
Sbjct: 256 ECYLPSGEPTRDNSRATARETFEQCPEEHPWFGLEQEYFIMGRDGRPYGWPAHGFP 311
Score = 52.0 bits (119), Expect = 2e-05
Identities = 21/44 (47%), Positives = 29/44 (65%)
Frame = +1
Query: 718 DAVFMLAYRLAGTNAEVMPLQWEFQVGPSIGVHAGDDLWVARYI 849
+ + ++GTNAEV P QWEFQ+GP G+ GD L VAR++
Sbjct: 337 EVCLQMGLNISGTNAEVTPGQWEFQIGPCEGLEMGDQLTVARWV 380
>UniRef50_UPI0000F20B38 Cluster: PREDICTED: hypothetical protein;
n=1; Danio rerio|Rep: PREDICTED: hypothetical protein -
Danio rerio
Length = 277
Score = 101 bits (242), Expect = 2e-20
Identities = 56/145 (38%), Positives = 81/145 (55%)
Frame = +2
Query: 182 SPNAVLSKTLLSRYNDLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNF 361
S ++ L+K L RY +LP D L TY+WID G L K RT++ PK D+P W+
Sbjct: 5 SESSHLNKFLRHRYLNLP-QGDFCLVTYVWIDSCGVDLYSKTRTMDCEPKILADVPEWDV 63
Query: 362 DGSSTNQADGHNSDTYLVPRAIYKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEAYD 541
G T ++ +S+ L +++DPF + L++C+ K+ EP E N+R C +
Sbjct: 64 -GLETEES---SSEMLLNHVRMFRDPFFLDPNKLILCEVLKHTREPAEWNHRNRCNTLME 119
Query: 542 KCKDDEPWFGIEQEYILLDSDLRPF 616
K KD PWFG+EQEY LL D P+
Sbjct: 120 KVKDLHPWFGMEQEYTLLGVDGHPY 144
>UniRef50_Q7M314 Cluster: Glutamate-ammonia ligase; n=1; Bos
taurus|Rep: Glutamate-ammonia ligase - Bos taurus
(Bovine)
Length = 149
Score = 89.8 bits (213), Expect = 8e-17
Identities = 56/144 (38%), Positives = 75/144 (52%)
Frame = +2
Query: 197 LSKTLLSRYNDLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSST 376
L K + Y LP DK+ A YIWIDG+GE LRCK RTL PK P +ST
Sbjct: 1 LBKGIKZVYMALP-QGDKVQAMYIWIDGTGEGLRCKTRTLXSXPKKP----------AST 49
Query: 377 NQADGHNSDTYLVPRAIYKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEAYDKCKDD 556
N + YLVP A+++DPF + LV C+ + YN P E+N +C +
Sbjct: 50 ------NLZRYLVPAAMFRDPFXXDPNXLVFCEVFXYNKRPAETNLXXTC------VSNQ 97
Query: 557 EPWFGIEQEYILLDSDLRPFRVAP 628
P FG+EQEY L+ + F++ P
Sbjct: 98 XPXFGMEQEYTLMPAQWE-FQIGP 120
>UniRef50_P20805 Cluster: Glutamine synthetase 2; n=19; Frankia|Rep:
Glutamine synthetase 2 - Frankia alni
Length = 352
Score = 84.2 bits (199), Expect = 4e-15
Identities = 50/120 (41%), Positives = 64/120 (53%)
Frame = +2
Query: 257 ATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAIYKD 436
A YIWIDG+ + +T I K K+ IW FDGSSTNQA G NSD L P D
Sbjct: 5 AEYIWIDGTEPEPLMRSKTR--IIKDGKEPEIWGFDGSSTNQAPGSNSDCVLRPVFETPD 62
Query: 437 PFRRGNHILVMCDTYKYNMEPTESNNRISCQEAYDKCKDDEPWFGIEQEYILLDSDLRPF 616
P R G++ LV+C+ + P +N R + ++ D P FGIEQEY D RP+
Sbjct: 63 PIRGGDNRLVLCEVQLTDFTP-PTNTRAAALGVAERYADMSPMFGIEQEYTFF-KDGRPY 120
Score = 44.0 bits (99), Expect = 0.005
Identities = 28/91 (30%), Positives = 43/91 (47%), Gaps = 4/91 (4%)
Frame = +1
Query: 589 FTGFRSAPLSGGPRVVSPHRKGH----TTAVSVPTKSLPETLSKPTIDAVFMLAYRLAGT 756
+T F+ G P V P +G +P + + E ++ +DA + GT
Sbjct: 111 YTFFKDGRPYGWPEVGYPAPQGPYYCGVGGSKMPGRQIVERHTQACLDA----GLAIEGT 166
Query: 757 NAEVMPLQWEFQVGPSIGVHAGDDLWVARYI 849
NAEVM QWEFQ+G GD +W+ R++
Sbjct: 167 NAEVMMGQWEFQIGVLPAPAIGDQIWLGRWL 197
>UniRef50_Q42689 Cluster: Glutamine synthetase, chloroplast
precursor; n=17; cellular organisms|Rep: Glutamine
synthetase, chloroplast precursor - Chlamydomonas
reinhardtii
Length = 380
Score = 83.0 bits (196), Expect = 9e-15
Identities = 45/124 (36%), Positives = 65/124 (52%), Gaps = 11/124 (8%)
Frame = +2
Query: 257 ATYIWIDGSG---------EHLRCKDRTLNF-IPKAPKDLPIWNFDGSSTNQADGHNSDT 406
A YIW DG+ +R K + + + P W+FDGSST QA+G+NSD
Sbjct: 37 AEYIWADGNEGKPEKGMIFNEMRSKTKCFEAPLGLDASEYPDWSFDGSSTGQAEGNNSDC 96
Query: 407 YLVPRAIYKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEAY-DKCKDDEPWFGIEQE 583
L P + DP R H+LVMC+ + + +P +N R +E DK ++ W+G EQE
Sbjct: 97 ILRPVRVVTDPIRGAPHVLVMCEVFAPDGKPHSTNTRAKLREIIDDKVTAEDCWYGFEQE 156
Query: 584 YILL 595
Y +L
Sbjct: 157 YTML 160
Score = 43.6 bits (98), Expect = 0.006
Identities = 18/50 (36%), Positives = 33/50 (66%)
Frame = +1
Query: 700 LSKPTIDAVFMLAYRLAGTNAEVMPLQWEFQVGPSIGVHAGDDLWVARYI 849
L++ ++A ++G NAEVMP QWE+Q+GP + GD++ ++R++
Sbjct: 196 LAEAHMEACMKAGLVISGINAEVMPGQWEYQIGPVGPLALGDEVMLSRWL 245
Score = 40.7 bits (91), Expect = 0.046
Identities = 16/30 (53%), Positives = 19/30 (63%)
Frame = +3
Query: 657 YYCGVGANKVFARDLVEAHYRCCLYAGVPI 746
+YCGVGA F R L EAH C+ AG+ I
Sbjct: 182 FYCGVGAESAFGRPLAEAHMEACMKAGLVI 211
>UniRef50_Q6N241 Cluster: Glutamine synthetase II; n=16;
Bacteria|Rep: Glutamine synthetase II - Rhodopseudomonas
palustris
Length = 345
Score = 77.4 bits (182), Expect = 4e-13
Identities = 50/130 (38%), Positives = 68/130 (52%), Gaps = 4/130 (3%)
Frame = +2
Query: 263 YIWIDG--SGEHLRCKDRTLNF-IPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAIYK 433
YIW+DG +LR K F I + LP+W FDGSST QA+GH+SD L P A+Y
Sbjct: 8 YIWLDGYKPTPNLRGKTTIKEFEIYPTLEQLPLWGFDGSSTMQAEGHSSDCVLKPVAMYP 67
Query: 434 DPFRRGNHILVMCDTYKYN-MEPTESNNRISCQEAYDKCKDDEPWFGIEQEYILLDSDLR 610
D R+ N ILV+C+ + + P +N R + + D+ WFG EQEY + R
Sbjct: 68 DAARK-NGILVLCEVMMPDGVTPHPTNTRATILD------DEGAWFGFEQEYFFYKNG-R 119
Query: 611 PFRVAPGWFP 640
P +P
Sbjct: 120 PLGFPEAGYP 129
Score = 37.1 bits (82), Expect = 0.56
Identities = 17/33 (51%), Positives = 20/33 (60%)
Frame = +1
Query: 751 GTNAEVMPLQWEFQVGPSIGVHAGDDLWVARYI 849
G NAEV QWEFQV A D +W+ARY+
Sbjct: 168 GINAEVAKGQWEFQVFGKGSRTAADQMWMARYL 200
>UniRef50_A0PCY1 Cluster: Glutamine synthetase precursor; n=1;
Guillardia theta|Rep: Glutamine synthetase precursor -
Guillardia theta (Cryptomonas phi)
Length = 160
Score = 77.4 bits (182), Expect = 4e-13
Identities = 41/90 (45%), Positives = 54/90 (60%), Gaps = 5/90 (5%)
Frame = +2
Query: 248 KILATYIWIDGSG---EHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVP 418
K A YIWI G G + R K R L+ P + +LP+WN+DGSST QA G +S+ YL P
Sbjct: 68 KCRAEYIWIGGRGGCGDDYRSKTRVLDKRPTSVSELPLWNYDGSSTGQAPGGDSEIYLQP 127
Query: 419 RAIYKDPFRRGNHILVMCDTY--KYNMEPT 502
+ DP R G++ILV+C+ K N PT
Sbjct: 128 AFMCADPMRGGDNILVLCEMIDPKNNPFPT 157
>UniRef50_P04772 Cluster: Glutamine synthetase 2; n=211;
Bacteria|Rep: Glutamine synthetase 2 - Bradyrhizobium
japonicum
Length = 344
Score = 76.6 bits (180), Expect = 7e-13
Identities = 50/130 (38%), Positives = 65/130 (50%), Gaps = 4/130 (3%)
Frame = +2
Query: 263 YIWIDG--SGEHLRCKDRTLNFIP-KAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAIYK 433
YIW+DG +LR K + F + LP+W FDGSST QA+GH+SD L P A++
Sbjct: 8 YIWLDGYTPTPNLRGKTQIKEFASFPTLEQLPLWGFDGSSTQQAEGHSSDCVLKPVAVFP 67
Query: 434 DPFRRGNHILVMCDTYKYN-MEPTESNNRISCQEAYDKCKDDEPWFGIEQEYILLDSDLR 610
D R N +LVMC+ + P SN R + + D WFG EQEY D R
Sbjct: 68 DA-ARTNGVLVMCEVMMPDGKTPHASNKRATILD------DAGAWFGFEQEYFFY-KDGR 119
Query: 611 PFRVAPGWFP 640
P +P
Sbjct: 120 PLGFPTSGYP 129
Score = 38.7 bits (86), Expect = 0.18
Identities = 27/82 (32%), Positives = 35/82 (42%), Gaps = 2/82 (2%)
Frame = +1
Query: 610 PLSGGPRVVSPHRKG--HTTAVSVPTKSLPETLSKPTIDAVFMLAYRLAGTNAEVMPLQW 783
P SG P P+ G + V K + E L D G NAEV QW
Sbjct: 124 PTSGYPAPQGPYYTGVGFSNVGDVARKIVEEHL-----DLCLAAGINHEGINAEVAKGQW 178
Query: 784 EFQVGPSIGVHAGDDLWVARYI 849
EFQ+ A D++W+ARY+
Sbjct: 179 EFQIFGKGSKKAADEMWMARYL 200
>UniRef50_P94845 Cluster: Glutamine synthetase; n=95;
Epsilonproteobacteria|Rep: Glutamine synthetase -
Helicobacter pylori (Campylobacter pylori)
Length = 481
Score = 40.7 bits (91), Expect = 0.046
Identities = 29/89 (32%), Positives = 40/89 (44%), Gaps = 8/89 (8%)
Frame = +2
Query: 359 FDGSSTNQADG-HNSDTYLVPRAI--YKDPFRRGNHILVMCDTYK-YNMEPTESNNRISC 526
FD S G +SD L P + + DPF ++V CD Y Y +P E R
Sbjct: 59 FDASCFKGWQGIEHSDMILTPDLVRYFIDPFSADVSVVVFCDVYDVYKNQPYEKCPRSIA 118
Query: 527 QEAYDKCKD----DEPWFGIEQEYILLDS 601
++A KD D +FG E E+ + DS
Sbjct: 119 KKALQHLKDSGLGDVAYFGAENEFFIFDS 147
>UniRef50_UPI000065D6C6 Cluster: Rho guanine nucleotide exchange
factor 10.; n=1; Takifugu rubripes|Rep: Rho guanine
nucleotide exchange factor 10. - Takifugu rubripes
Length = 987
Score = 37.1 bits (82), Expect = 0.56
Identities = 25/65 (38%), Positives = 38/65 (58%), Gaps = 3/65 (4%)
Frame = -3
Query: 255 RILSAGSGRSLYRLSSVLDSTAF--GELVKTGPDRIFGLSSILVCAVSAI-LKQVRHDYV 85
++LS GS R L R ++++ GE++KT R+F L+ +L+CA I L D V
Sbjct: 327 KLLSNGS-RYLIRSDDMIETVYSDRGEIIKTKERRLFLLNDVLMCATPNIRLPLSASDLV 385
Query: 84 LKCSL 70
LKC+L
Sbjct: 386 LKCAL 390
>UniRef50_A5C4G8 Cluster: Putative uncharacterized protein; n=1;
Vitis vinifera|Rep: Putative uncharacterized protein -
Vitis vinifera (Grape)
Length = 443
Score = 35.9 bits (79), Expect = 1.3
Identities = 19/49 (38%), Positives = 26/49 (53%)
Frame = +1
Query: 655 HTTAVSVPTKSLPETLSKPTIDAVFMLAYRLAGTNAEVMPLQWEFQVGP 801
+ V+V TK + + I A ++G N EVMP QWE+QVGP
Sbjct: 278 NVAVVTVKTKRTIQLKTSLEI-ACHYAGINISGINGEVMPGQWEYQVGP 325
>UniRef50_A5B590 Cluster: Putative uncharacterized protein; n=1;
Vitis vinifera|Rep: Putative uncharacterized protein -
Vitis vinifera (Grape)
Length = 396
Score = 35.5 bits (78), Expect = 1.7
Identities = 22/52 (42%), Positives = 28/52 (53%), Gaps = 1/52 (1%)
Frame = +1
Query: 649 KGHTTAVS-VPTKSLPETLSKPTIDAVFMLAYRLAGTNAEVMPLQWEFQVGP 801
KG AV+ V TK + + I A ++G N EVMP QWE+QVGP
Sbjct: 177 KGVNXAVATVKTKRTIQLKTSLEI-ACHYAGINISGINGEVMPGQWEYQVGP 227
>UniRef50_Q9BIT1 Cluster: Fibroin 2; n=1; Plectreurys tristis|Rep:
Fibroin 2 - Plectreurys tristis (Spider)
Length = 764
Score = 34.3 bits (75), Expect = 4.0
Identities = 22/65 (33%), Positives = 37/65 (56%)
Frame = -3
Query: 246 SAGSGRSLYRLSSVLDSTAFGELVKTGPDRIFGLSSILVCAVSAILKQVRHDYVLKCSLD 67
SAGSG SLY LS++L TA + K+ P+ + +L+ +++ I+ + + S
Sbjct: 698 SAGSGLSLYTLSNLLSQTALA-ISKSRPE--LSPNEVLIQSLAEIIVALVQALTKQASSS 754
Query: 66 ASVEY 52
ASV+Y
Sbjct: 755 ASVQY 759
>UniRef50_Q6ZUE9 Cluster: CDNA FLJ43768 fis, clone TESTI2049422;
n=2; Homo sapiens|Rep: CDNA FLJ43768 fis, clone
TESTI2049422 - Homo sapiens (Human)
Length = 290
Score = 34.3 bits (75), Expect = 4.0
Identities = 24/75 (32%), Positives = 34/75 (45%), Gaps = 1/75 (1%)
Frame = +1
Query: 592 TGFRSAPLSG-GPRVVSPHRKGHTTAVSVPTKSLPETLSKPTIDAVFMLAYRLAGTNAEV 768
TG R AP GPR+ SP R+ T P++ P S+P + + +++R GT V
Sbjct: 203 TGPRQAPCQAPGPRLASPPRRKQTGLWLPPSEPTPSLFSRPEL-LIPSISFRPLGTYPRV 261
Query: 769 MPLQWEFQVGPSIGV 813
L G GV
Sbjct: 262 DALSHAMGSGMGWGV 276
>UniRef50_A7IAX6 Cluster: Putative uncharacterized protein
precursor; n=1; Candidatus Methanoregula boonei 6A8|Rep:
Putative uncharacterized protein precursor -
Methanoregula boonei (strain 6A8)
Length = 194
Score = 33.9 bits (74), Expect = 5.2
Identities = 20/74 (27%), Positives = 29/74 (39%), Gaps = 3/74 (4%)
Frame = -1
Query: 812 TPIEGPTWNSHCKGITS--AFVP-ANRYASIKTASIVGFDKVSGKDFVGTDTAVVWPLRW 642
T G W + G AF P ++ Y ++ I+G +G F T WP W
Sbjct: 100 TDANGNAWLNVTDGTIGVPAFAPGSSMYINVTNLQIIGSQSGAGMLFATTGHGSQWPTWW 159
Query: 641 GETTRGPPERGADL 600
E+ P G D+
Sbjct: 160 AESLMNPANVGKDI 173
>UniRef50_UPI0000DA3C43 Cluster: PREDICTED: similar to VPS10 domain
receptor protein SORCS 2; n=1; Rattus norvegicus|Rep:
PREDICTED: similar to VPS10 domain receptor protein
SORCS 2 - Rattus norvegicus
Length = 1160
Score = 33.5 bits (73), Expect = 6.9
Identities = 16/30 (53%), Positives = 18/30 (60%)
Frame = +2
Query: 656 ILLRCRCQQSLCQRPCRSPLSMLSLCWRTD 745
I LR CQQ LCQ + P+S LC RTD
Sbjct: 716 IFLRVYCQQVLCQLLVQPPVSSXRLCPRTD 745
>UniRef50_A4CQL3 Cluster: Putative uncharacterized protein; n=1;
Synechococcus sp. WH 7805|Rep: Putative uncharacterized
protein - Synechococcus sp. (strain WH7805)
Length = 227
Score = 33.5 bits (73), Expect = 6.9
Identities = 29/87 (33%), Positives = 40/87 (45%), Gaps = 3/87 (3%)
Frame = -1
Query: 812 TPIEGPTWNSHCKGITSAFVPANRYASIKTASIVGFDKVSGKD---FVGTDTAVVWPLRW 642
TP +GP+W + +A P S+ S V D+V KD ++G D A+ W
Sbjct: 32 TPRDGPSWKAMVPDRWAAVGPVPEALSLSEDSRVHLDQVPLKDVPPWLGIDRALA---GW 88
Query: 641 GETTRGPPERGADLNPVKYIPVLSQTR 561
G +R G DL+ V VLS TR
Sbjct: 89 GAWSRS--SGGEDLSVVDAGTVLSLTR 113
>UniRef50_Q8ILR9 Cluster: Putative uncharacterized protein; n=1;
Plasmodium falciparum 3D7|Rep: Putative uncharacterized
protein - Plasmodium falciparum (isolate 3D7)
Length = 4662
Score = 33.5 bits (73), Expect = 6.9
Identities = 21/64 (32%), Positives = 33/64 (51%), Gaps = 6/64 (9%)
Frame = +2
Query: 431 KDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEA------YDKCKDDEPWFGIEQEYIL 592
K+ N+IL+ DT+K E ++ N+ C + Y+KCKDDE + + YIL
Sbjct: 2427 KNSTNENNNILINNDTFKSKDEKKDTYNKEVCVNSISQLIIYNKCKDDE--YKYDFNYIL 2484
Query: 593 LDSD 604
+ D
Sbjct: 2485 KNRD 2488
>UniRef50_Q5A3J6 Cluster: Potential CCR4-NOT complex associated
factor Caf120p; n=1; Candida albicans|Rep: Potential
CCR4-NOT complex associated factor Caf120p - Candida
albicans (Yeast)
Length = 1021
Score = 33.5 bits (73), Expect = 6.9
Identities = 16/50 (32%), Positives = 26/50 (52%)
Frame = +2
Query: 146 NPKILSGPVLTNSPNAVLSKTLLSRYNDLPLPADKILATYIWIDGSGEHL 295
N +LS + N+ +V + +Y+DL P+D I ++GS EHL
Sbjct: 491 NDNLLSADKMKNNHKSVQLAEIYQKYSDLKTPSDNYTDRNILLNGSHEHL 540
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 946,469,366
Number of Sequences: 1657284
Number of extensions: 21383161
Number of successful extensions: 61789
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 58523
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 61749
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 75013275813
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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