Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0185
(851 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g48470.1 68414.m05418 glutamine synthetase, putative similar ... 140 1e-33
At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to... 137 1e-32
At5g16570.1 68418.m01939 glutamine synthetase, putative similar ... 133 1e-31
At5g37600.1 68418.m04529 glutamine synthetase, putative similar ... 133 2e-31
At1g66200.1 68414.m07514 glutamine synthetase, putative similar ... 132 3e-31
At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to... 129 2e-30
At3g14270.1 68416.m01806 phosphatidylinositol-4-phosphate 5-kina... 29 3.9
At5g57130.1 68418.m07135 expressed protein 29 5.2
At3g15370.1 68416.m01949 expansin, putative (EXP12) similar to e... 29 5.2
At2g41590.1 68415.m05139 expressed protein similar to zinc finge... 29 5.2
At4g11540.1 68417.m01851 DC1 domain-containing protein contains ... 28 6.9
At5g49270.1 68418.m06098 phytochelatin synthetase-related contai... 28 9.1
At4g32880.1 68417.m04679 homeobox-leucine zipper transcription f... 28 9.1
>At1g48470.1 68414.m05418 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase, GS1) [Arabidopsis thaliana]
SWISS-PROT:Q9LVI8
Length = 353
Score = 140 bits (339), Expect = 1e-33
Identities = 70/134 (52%), Positives = 88/134 (65%), Gaps = 3/134 (2%)
Frame = +2
Query: 248 KILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAI 427
KI+A YIWI GSG +R K RTL P LP WN+DGSST+QA G +S+ L P+AI
Sbjct: 18 KIIAEYIWIGGSGMDIRSKARTLPGPVSNPTKLPKWNYDGSSTDQAAGDDSEVILYPQAI 77
Query: 428 YKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEAYD--KCKDDEPWFGIEQEYILLDS 601
+KDPFR+GN+ILVMCD Y+ +P +NNR + +D K +EPWFGIEQEY LL
Sbjct: 78 FKDPFRKGNNILVMCDAYRPAGDPIPTNNRHKAVKIFDHPNVKAEEPWFGIEQEYTLLKK 137
Query: 602 DLR-PFRVAPGWFP 640
D++ P G FP
Sbjct: 138 DVKWPLGWPLGGFP 151
Score = 60.1 bits (139), Expect = 2e-09
Identities = 23/35 (65%), Positives = 28/35 (80%)
Frame = +1
Query: 745 LAGTNAEVMPLQWEFQVGPSIGVHAGDDLWVARYI 849
+ G N EVMP QWEFQ+ P++G+ AGD LWVARYI
Sbjct: 186 IGGANGEVMPGQWEFQISPTVGIGAGDQLWVARYI 220
Score = 54.8 bits (126), Expect = 7e-08
Identities = 20/31 (64%), Positives = 26/31 (83%)
Frame = +3
Query: 657 YYCGVGANKVFARDLVEAHYRCCLYAGVPIG 749
YYC VGA+K F RD+V+AHY+ CLY+G+ IG
Sbjct: 157 YYCAVGADKAFGRDIVDAHYKACLYSGLSIG 187
>At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to
glutamine synthetase, chloroplast precursor (glutamate--
ammonia ligase, GS2) [Arabidopsis thaliana]
SWISS-PROT:Q43127
Length = 430
Score = 137 bits (331), Expect = 1e-32
Identities = 74/173 (42%), Positives = 101/173 (58%), Gaps = 4/173 (2%)
Frame = +2
Query: 134 KIEDNPKILSGPVLT-NSPNAVLSKTLLSRYNDLPLPADKILATYIWIDGSGEHLRCKDR 310
K + N K+ VL S N+ +++ D +D+I+A YIWI GSG LR K R
Sbjct: 37 KKQSNNKVRGFRVLALQSDNSTVNRVETLLNLDTKPYSDRIIAEYIWIGGSGIDLRSKSR 96
Query: 311 TLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAIYKDPFRRGNHILVMCDTYKYN 490
T+ + P +LP WN+DGSST QA G +S+ L P+AI++DPFR GN+ILV+CDT+
Sbjct: 97 TIEKPVEDPSELPKWNYDGSSTGQAPGEDSEVILYPQAIFRDPFRGGNNILVICDTWTPA 156
Query: 491 MEPTESNNRISCQEAYD--KCKDDEPWFGIEQEYILLDSDLR-PFRVAPGWFP 640
EP +N R E + K + PWFGIEQEY LL +++ P G FP
Sbjct: 157 GEPIPTNKRAKAAEIFSNKKVSGEVPWFGIEQEYTLLQQNVKWPLGWPVGAFP 209
Score = 62.5 bits (145), Expect = 3e-10
Identities = 24/35 (68%), Positives = 30/35 (85%)
Frame = +1
Query: 745 LAGTNAEVMPLQWEFQVGPSIGVHAGDDLWVARYI 849
++GTN EVMP QWEFQVGPS+G+ AGD +W ARY+
Sbjct: 244 ISGTNGEVMPGQWEFQVGPSVGIDAGDHVWCARYL 278
Score = 53.2 bits (122), Expect = 2e-07
Identities = 19/30 (63%), Positives = 26/30 (86%)
Frame = +3
Query: 657 YYCGVGANKVFARDLVEAHYRCCLYAGVPI 746
YYCGVGA+K++ RD+ +AHY+ CLYAG+ I
Sbjct: 215 YYCGVGADKIWGRDISDAHYKACLYAGINI 244
>At5g16570.1 68418.m01939 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase) [Alfalfa] SWISS-PROT:P04078
Length = 356
Score = 133 bits (322), Expect = 1e-31
Identities = 68/141 (48%), Positives = 87/141 (61%), Gaps = 3/141 (2%)
Frame = +2
Query: 227 DLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDT 406
DL D+I+A YIWI GSG +R K RTL P LP WN+DGSST QA G +S+
Sbjct: 11 DLSDSTDQIIAEYIWIGGSGLDMRSKARTLPGPVTDPSQLPKWNYDGSSTGQAPGDDSEV 70
Query: 407 YLVPRAIYKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEAYD--KCKDDEPWFGIEQ 580
+ P+AI+KDPFRRGN+ILVMCD Y EP +N R + + ++ +E W+GIEQ
Sbjct: 71 IIYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAKIFEDPSVVAEETWYGIEQ 130
Query: 581 EYILLDSDLR-PFRVAPGWFP 640
EY LL D++ P G FP
Sbjct: 131 EYTLLQKDIKWPVGWPVGGFP 151
Score = 61.3 bits (142), Expect = 8e-10
Identities = 24/35 (68%), Positives = 30/35 (85%)
Frame = +1
Query: 745 LAGTNAEVMPLQWEFQVGPSIGVHAGDDLWVARYI 849
++GTN EVMP QWEFQVGP++G+ A D +WVARYI
Sbjct: 186 VSGTNGEVMPGQWEFQVGPTVGIAAADQVWVARYI 220
Score = 54.8 bits (126), Expect = 7e-08
Identities = 19/30 (63%), Positives = 26/30 (86%)
Frame = +3
Query: 657 YYCGVGANKVFARDLVEAHYRCCLYAGVPI 746
YYCGVGA+K F RD+V++HY+ CLYAG+ +
Sbjct: 157 YYCGVGADKAFGRDIVDSHYKACLYAGINV 186
>At5g37600.1 68418.m04529 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (Glutamate--
ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899
Length = 356
Score = 133 bits (321), Expect = 2e-31
Identities = 63/124 (50%), Positives = 80/124 (64%), Gaps = 2/124 (1%)
Frame = +2
Query: 245 DKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRA 424
DKI+A YIW+ GSG +R K RTL P LP WN+DGSST QA G +S+ L P+A
Sbjct: 17 DKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSQLPKWNYDGSSTGQAPGEDSEVILYPQA 76
Query: 425 IYKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQEAYDK--CKDDEPWFGIEQEYILLD 598
I+KDPFRRGN+ILVMCD Y EP +N R + + + + PW+GIEQEY LL
Sbjct: 77 IFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAKVFSNPDVAAEVPWYGIEQEYTLLQ 136
Query: 599 SDLR 610
D++
Sbjct: 137 KDVK 140
Score = 60.9 bits (141), Expect = 1e-09
Identities = 23/35 (65%), Positives = 30/35 (85%)
Frame = +1
Query: 745 LAGTNAEVMPLQWEFQVGPSIGVHAGDDLWVARYI 849
++G N EVMP QWEFQVGP++G+ A D++WVARYI
Sbjct: 186 ISGINGEVMPGQWEFQVGPAVGISAADEIWVARYI 220
Score = 53.6 bits (123), Expect = 2e-07
Identities = 19/30 (63%), Positives = 26/30 (86%)
Frame = +3
Query: 657 YYCGVGANKVFARDLVEAHYRCCLYAGVPI 746
YYCG+GA+K F RD+V++HY+ CLYAG+ I
Sbjct: 157 YYCGIGADKSFGRDVVDSHYKACLYAGINI 186
>At1g66200.1 68414.m07514 glutamine synthetase, putative similar to
glutamine synthetase, cytosolic isozyme (Glutamate--
ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899
Length = 356
Score = 132 bits (319), Expect = 3e-31
Identities = 69/141 (48%), Positives = 86/141 (60%), Gaps = 3/141 (2%)
Frame = +2
Query: 227 DLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDT 406
D+ ++KI+A YIW+ GSG +R K RTL P LP WN+DGSST QA G +S+
Sbjct: 11 DISDNSEKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSKLPKWNYDGSSTGQAPGQDSEV 70
Query: 407 YLVPRAIYKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQE--AYDKCKDDEPWFGIEQ 580
L P+AI+KDPFRRGN+ILVMCD Y EP +N R + E A + PW+GIEQ
Sbjct: 71 ILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAEIFANPDVIAEVPWYGIEQ 130
Query: 581 EYILLDSDLR-PFRVAPGWFP 640
EY LL D+ P G FP
Sbjct: 131 EYTLLQKDVNWPLGWPIGGFP 151
Score = 61.7 bits (143), Expect = 6e-10
Identities = 23/35 (65%), Positives = 30/35 (85%)
Frame = +1
Query: 745 LAGTNAEVMPLQWEFQVGPSIGVHAGDDLWVARYI 849
++G N EVMP QWEFQVGPS+G+ A D++W+ARYI
Sbjct: 186 ISGINGEVMPGQWEFQVGPSVGISAADEIWIARYI 220
Score = 48.8 bits (111), Expect = 5e-06
Identities = 18/30 (60%), Positives = 24/30 (80%)
Frame = +3
Query: 657 YYCGVGANKVFARDLVEAHYRCCLYAGVPI 746
YYC +GA+K F RD+V+AHY+ LYAG+ I
Sbjct: 157 YYCSIGADKSFGRDIVDAHYKASLYAGINI 186
>At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to
glutamine synthetase, cytosolic isozyme (glutamate--
ammonia ligase, GS1) [Arabidopsis thaliana]
SWISS-PROT:Q9LVI8
Length = 354
Score = 129 bits (312), Expect = 2e-30
Identities = 64/134 (47%), Positives = 84/134 (62%), Gaps = 3/134 (2%)
Frame = +2
Query: 248 KILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAI 427
KI+A YIWI GSG +R K RTL P LP WN+DGSST QA G +S+ L P+AI
Sbjct: 18 KIIAEYIWIGGSGMDIRSKARTLPGPVTDPSKLPKWNYDGSSTGQAAGEDSEVILYPQAI 77
Query: 428 YKDPFRRGNHILVMCDTYKYNMEPTESNNRISCQE--AYDKCKDDEPWFGIEQEYILLDS 601
+KDPFR+GN+ILVMCD Y +P +N R + + ++ +EPW+GIEQEY L+
Sbjct: 78 FKDPFRKGNNILVMCDAYTPAGDPIPTNKRHNAAKIFSHPDVAKEEPWYGIEQEYTLMQK 137
Query: 602 DLR-PFRVAPGWFP 640
D+ P G +P
Sbjct: 138 DVNWPIGWPVGGYP 151
Score = 56.4 bits (130), Expect = 2e-08
Identities = 22/35 (62%), Positives = 28/35 (80%)
Frame = +1
Query: 745 LAGTNAEVMPLQWEFQVGPSIGVHAGDDLWVARYI 849
++G N EVMP QWEFQVGP G+ +GD +WVARY+
Sbjct: 186 ISGINGEVMPGQWEFQVGPVEGISSGDQVWVARYL 220
Score = 54.0 bits (124), Expect = 1e-07
Identities = 20/30 (66%), Positives = 25/30 (83%)
Frame = +3
Query: 657 YYCGVGANKVFARDLVEAHYRCCLYAGVPI 746
YYCGVGA+K RD+V+AHY+ CLYAG+ I
Sbjct: 157 YYCGVGADKAIGRDIVDAHYKACLYAGIGI 186
>At3g14270.1 68416.m01806 phosphatidylinositol-4-phosphate 5-kinase
family protein similar to SP|Q9Z1T6 FYVE
finger-containing phosphoinositide kinase (EC 2.7.1.68)
(1- phosphatidylinositol-4-phosphate kinase) (PIP5K)
(PtdIns(4)P-5-kinase) {Mus musculus}; contains Pfam
profiles PF01504: Phosphatidylinositol-4-phosphate
5-Kinase, PF01363: FYVE zinc finger, PF00118: TCP-1/cpn60
chaperonin family
Length = 1791
Score = 29.1 bits (62), Expect = 3.9
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +1
Query: 571 DRTGIYFTGFRSAPLSGGPRVVSP 642
+ + IY + FR A L GGPR++ P
Sbjct: 1366 EHSPIYISSFREAELQGGPRLLLP 1389
>At5g57130.1 68418.m07135 expressed protein
Length = 920
Score = 28.7 bits (61), Expect = 5.2
Identities = 17/47 (36%), Positives = 24/47 (51%)
Frame = -3
Query: 729 KDSIDSGLRQGLWQRLCWHRHRSSMALAVGGNHPGATRKGRRSESSK 589
KD + GL++ W R C H + L++ GN+P G ESSK
Sbjct: 444 KDEL-MGLKRK-WNRFCETLHNQTGQLSMMGNYPYGLPYGSSHESSK 488
>At3g15370.1 68416.m01949 expansin, putative (EXP12) similar to
expansin GI:11191999 from [Lycopersicon esculentum];
alpha-expansin gene family, PMID:11641069
Length = 252
Score = 28.7 bits (61), Expect = 5.2
Identities = 16/49 (32%), Positives = 23/49 (46%)
Frame = -2
Query: 448 STEWILVNSTRYEVGIRIVPISLVG*AAIEVPYRQIFGSFRYEIQGAIF 302
S WI ++T Y G+ P SL G + PY FG+ + G +F
Sbjct: 24 SNGWIRAHATYY--GVNDSPASLGGACGYDNPYHAGFGAHTAALSGELF 70
>At2g41590.1 68415.m05139 expressed protein similar to zinc finger
protein [Arabidopsis thaliana] GI:976277
Length = 367
Score = 28.7 bits (61), Expect = 5.2
Identities = 13/36 (36%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = +2
Query: 218 RYNDLPLPADKILATYIWID-GSGEHLRCKDRTLNF 322
R + PL D+ +A Y +D G +H+RC+ + NF
Sbjct: 263 RISHPPLNHDEFVAAYPHLDSGRNDHIRCEGESSNF 298
>At4g11540.1 68417.m01851 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 525
Score = 28.3 bits (60), Expect = 6.9
Identities = 19/63 (30%), Positives = 28/63 (44%)
Frame = -1
Query: 401 QNCAHQLGWLSCHRSSI*ADLWEL*V*NSRCDLCTSGVRQSRRSKCRWRESCQPAVEDHC 222
Q+ +H+ +L H + + LWE N RC CT VR C+ C + HC
Sbjct: 286 QHFSHKEHYLRFHVNGL---LWEE---NKRCSACTHPVRLQSFYACK---DCDFILHQHC 336
Query: 221 IDS 213
+S
Sbjct: 337 AES 339
>At5g49270.1 68418.m06098 phytochelatin synthetase-related contains
Pfam PF04833: Phytochelatin synthetase-like conserved
region
Length = 663
Score = 27.9 bits (59), Expect = 9.1
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = -2
Query: 487 VFIRITHNEDVISSTEWILVNST 419
VF+ HNE +IS+T+ ++VN T
Sbjct: 91 VFVGFQHNEILISATDALIVNGT 113
>At4g32880.1 68417.m04679 homeobox-leucine zipper transcription
factor (HB-8) identical to HD-zip transcription factor
(athb-8) (GI:7270235) [Arabidopsis thaliana]
Length = 833
Score = 27.9 bits (59), Expect = 9.1
Identities = 14/34 (41%), Positives = 17/34 (50%)
Frame = +1
Query: 265 HLDRRLWRTPEVQRSHLEFHT*SSQRSAYMELRW 366
H D W PEV RS E T +QR+ LR+
Sbjct: 332 HFDLEPWSVPEVLRSLYESSTLLAQRTTMAALRY 365
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,410,726
Number of Sequences: 28952
Number of extensions: 471497
Number of successful extensions: 1325
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1261
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1319
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1980143200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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