Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0170
(717 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g10850.1 68416.m01307 hydroxyacylglutathione hydrolase, cytop... 132 2e-31
At2g31350.2 68415.m03830 hydroxyacylglutathione hydrolase, putat... 80 1e-15
At2g31350.1 68415.m03829 hydroxyacylglutathione hydrolase, putat... 80 1e-15
At1g06130.2 68414.m00643 hydroxyacylglutathione hydrolase, putat... 80 2e-15
At1g06130.1 68414.m00642 hydroxyacylglutathione hydrolase, putat... 80 2e-15
At2g43430.2 68415.m05397 hydroxyacylglutathione hydrolase, mitoc... 75 5e-14
At2g43430.1 68415.m05398 hydroxyacylglutathione hydrolase, mitoc... 75 5e-14
At1g53580.2 68414.m06085 hydroxyacylglutathione hydrolase, putat... 49 3e-06
At1g53580.1 68414.m06084 hydroxyacylglutathione hydrolase, putat... 49 3e-06
At3g59550.1 68416.m06646 cohesion family protein SYN3 (SYN3) nea... 28 7.1
At5g27700.1 68418.m03322 40S ribosomal protein S21 (RPS21C) ribo... 27 9.4
At3g53890.1 68416.m05953 40S ribosomal protein S21 (RPS21B) ribo... 27 9.4
At2g25730.1 68415.m03084 expressed protein 27 9.4
>At3g10850.1 68416.m01307 hydroxyacylglutathione hydrolase,
cytoplasmic / glyoxalase II (GLX2-2) identical to
SP|O24496 Hydroxyacylglutathione hydrolase cytoplasmic
(EC 3.1.2.6) (Glyoxalase II) (Glx II) {Arabidopsis
thaliana}
Length = 258
Score = 132 bits (319), Expect = 2e-31
Identities = 63/147 (42%), Positives = 92/147 (62%), Gaps = 2/147 (1%)
Frame = +1
Query: 274 MDVKILPALQDNYMYLIVDKATKEAAIVDPVEPKTVLKAVEEQGVNLXXXXXXXXXXXXA 453
M + +P LQDNY YLI+D++T +AA+VDPV+P+ V+ + E+ + A
Sbjct: 1 MKIFHVPCLQDNYSYLIIDESTGDAAVVDPVDPEKVIASAEKHQAKIKFVLTTHHHWDHA 60
Query: 454 GGNEDLIKERPGLIVYGGD-DRIGALTKKVEHNTKFKIG-NLNVQCLFTPCHTTGHICYF 627
GGNE + + P + VYGG D++ T V++ K +G ++N+ L TPCHT GHI Y+
Sbjct: 61 GGNEKIKQLVPDIKVYGGSLDKVKGCTDAVDNGDKLTLGQDINILALHTPCHTKGHISYY 120
Query: 628 VTAPEEGNDSVVFTGDTLFLGGCGRFF 708
V +EG + VFTGDTLF+ GCG+FF
Sbjct: 121 VNG-KEGENPAVFTGDTLFVAGCGKFF 146
>At2g31350.2 68415.m03830 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
isozyme [Arabidopsis thaliana] gi|2570338|gb|AAC49865
Length = 323
Score = 80.2 bits (189), Expect = 1e-15
Identities = 45/149 (30%), Positives = 73/149 (48%), Gaps = 3/149 (2%)
Frame = +1
Query: 271 NMDVKILPALQDNYMYLIVDKATKEAAIVDPVEPKTVLKAVEEQGVNLXXXXXXXXXXXX 450
++ ++++P L+DNY Y++ D+ T +VDP E + ++ +++ G NL
Sbjct: 69 SLQIELVPCLKDNYAYILHDEDTGTVGVVDPSEAEPIIDSLKRSGRNLTYILNTHHHYDH 128
Query: 451 AGGNEDLIKERPGLIVYGG---DDRIGALTKKVEHNTKFKIGNLNVQCLFTPCHTTGHIC 621
GGN +L K+R G V G DRI + ++ K+ V + TP HT GHI
Sbjct: 129 TGGNLEL-KDRYGAKVIGSAMDKDRIPGIDMALKDGDKWMFAGHEVHVMDTPGHTKGHIS 187
Query: 622 YFVTAPEEGNDSVVFTGDTLFLGGCGRFF 708
+ +FTGDT+F CG+ F
Sbjct: 188 LYFP-----GSRAIFTGDTMFSLSCGKLF 211
>At2g31350.1 68415.m03829 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
isozyme [Arabidopsis thaliana] gi|2570338|gb|AAC49865
Length = 324
Score = 80.2 bits (189), Expect = 1e-15
Identities = 45/149 (30%), Positives = 73/149 (48%), Gaps = 3/149 (2%)
Frame = +1
Query: 271 NMDVKILPALQDNYMYLIVDKATKEAAIVDPVEPKTVLKAVEEQGVNLXXXXXXXXXXXX 450
++ ++++P L+DNY Y++ D+ T +VDP E + ++ +++ G NL
Sbjct: 70 SLQIELVPCLKDNYAYILHDEDTGTVGVVDPSEAEPIIDSLKRSGRNLTYILNTHHHYDH 129
Query: 451 AGGNEDLIKERPGLIVYGG---DDRIGALTKKVEHNTKFKIGNLNVQCLFTPCHTTGHIC 621
GGN +L K+R G V G DRI + ++ K+ V + TP HT GHI
Sbjct: 130 TGGNLEL-KDRYGAKVIGSAMDKDRIPGIDMALKDGDKWMFAGHEVHVMDTPGHTKGHIS 188
Query: 622 YFVTAPEEGNDSVVFTGDTLFLGGCGRFF 708
+ +FTGDT+F CG+ F
Sbjct: 189 LYFP-----GSRAIFTGDTMFSLSCGKLF 212
>At1g06130.2 68414.m00643 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
isozyme GB:AAC49865 GI:2570338 from [Arabidopsis
thaliana]
Length = 330
Score = 79.8 bits (188), Expect = 2e-15
Identities = 56/187 (29%), Positives = 87/187 (46%), Gaps = 10/187 (5%)
Frame = +1
Query: 178 GLSQRITKLYFRALIRNQREAHSSQEDHQY-------KNMDVKILPALQDNYMYLIVDKA 336
GL + KL F + +R R+A S ++ ++ ++++P L DNY Y++ D+
Sbjct: 37 GLVSGVMKL-FSSPLRTLRDAGKSVRISRFCSVSNVSSSLQIELVPCLTDNYAYILHDED 95
Query: 337 TKEAAIVDPVEPKTVLKAVEEQGVNLXXXXXXXXXXXXAGGNEDLIKERPGLIVYGG--- 507
T +VDP E V+ A+++ NL GGN +L K+R G V G
Sbjct: 96 TGTVGVVDPSEAVPVMDALQKNSRNLTYILNTHHHYDHTGGNLEL-KDRYGAKVIGSAAD 154
Query: 508 DDRIGALTKKVEHNTKFKIGNLNVQCLFTPCHTTGHICYFVTAPEEGNDSVVFTGDTLFL 687
DRI + ++ K+ V + TP HT GHI ++ +FTGDTLF
Sbjct: 155 RDRIPGIDVALKDADKWMFAGHEVHIMETPGHTRGHISFYFPGAR-----AIFTGDTLFS 209
Query: 688 GGCGRFF 708
CG+ F
Sbjct: 210 LSCGKLF 216
>At1g06130.1 68414.m00642 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
isozyme GB:AAC49865 GI:2570338 from [Arabidopsis
thaliana]
Length = 331
Score = 79.8 bits (188), Expect = 2e-15
Identities = 56/187 (29%), Positives = 87/187 (46%), Gaps = 10/187 (5%)
Frame = +1
Query: 178 GLSQRITKLYFRALIRNQREAHSSQEDHQY-------KNMDVKILPALQDNYMYLIVDKA 336
GL + KL F + +R R+A S ++ ++ ++++P L DNY Y++ D+
Sbjct: 38 GLVSGVMKL-FSSPLRTLRDAGKSVRISRFCSVSNVSSSLQIELVPCLTDNYAYILHDED 96
Query: 337 TKEAAIVDPVEPKTVLKAVEEQGVNLXXXXXXXXXXXXAGGNEDLIKERPGLIVYGG--- 507
T +VDP E V+ A+++ NL GGN +L K+R G V G
Sbjct: 97 TGTVGVVDPSEAVPVMDALQKNSRNLTYILNTHHHYDHTGGNLEL-KDRYGAKVIGSAAD 155
Query: 508 DDRIGALTKKVEHNTKFKIGNLNVQCLFTPCHTTGHICYFVTAPEEGNDSVVFTGDTLFL 687
DRI + ++ K+ V + TP HT GHI ++ +FTGDTLF
Sbjct: 156 RDRIPGIDVALKDADKWMFAGHEVHIMETPGHTRGHISFYFPGAR-----AIFTGDTLFS 210
Query: 688 GGCGRFF 708
CG+ F
Sbjct: 211 LSCGKLF 217
>At2g43430.2 68415.m05397 hydroxyacylglutathione hydrolase,
mitochondrial / glyoxalase II (GLX2-1) identical to
SP|O24495 Hydroxyacylglutathione hydrolase,
mitochondrial precursor (EC 3.1.2.6) (Glyoxalase II)
(Glx II) {Arabidopsis thaliana}
Length = 313
Score = 74.9 bits (176), Expect = 5e-14
Identities = 45/146 (30%), Positives = 72/146 (49%), Gaps = 3/146 (2%)
Frame = +1
Query: 271 NMDVKILPALQDNYMYLIVDKATKEAAIVDPVEPKTVLKAVEEQGVNLXXXXXXXXXXXX 450
++ ++++P +DNY YL+ D+ T +VDP E V++A+ + NL
Sbjct: 59 SLKIELVPCSKDNYAYLLHDEDTGTVGVVDPSEAAPVIEALSRKNWNLTYILNTHHHDDH 118
Query: 451 AGGNEDLIKERPGLIVYGG---DDRIGALTKKVEHNTKFKIGNLNVQCLFTPCHTTGHIC 621
GGN +L KER G V G DRI + ++ + K+ V+ L TP HT GHI
Sbjct: 119 IGGNAEL-KERYGAKVIGSAVDKDRIPGIDILLKDSDKWMFAGHEVRILDTPGHTQGHIS 177
Query: 622 YFVTAPEEGNDSVVFTGDTLFLGGCG 699
++ + +FTGD ++ CG
Sbjct: 178 FYFP-----GSATIFTGDLIYSLSCG 198
>At2g43430.1 68415.m05398 hydroxyacylglutathione hydrolase,
mitochondrial / glyoxalase II (GLX2-1) identical to
SP|O24495 Hydroxyacylglutathione hydrolase,
mitochondrial precursor (EC 3.1.2.6) (Glyoxalase II)
(Glx II) {Arabidopsis thaliana}
Length = 331
Score = 74.9 bits (176), Expect = 5e-14
Identities = 45/146 (30%), Positives = 72/146 (49%), Gaps = 3/146 (2%)
Frame = +1
Query: 271 NMDVKILPALQDNYMYLIVDKATKEAAIVDPVEPKTVLKAVEEQGVNLXXXXXXXXXXXX 450
++ ++++P +DNY YL+ D+ T +VDP E V++A+ + NL
Sbjct: 77 SLKIELVPCSKDNYAYLLHDEDTGTVGVVDPSEAAPVIEALSRKNWNLTYILNTHHHDDH 136
Query: 451 AGGNEDLIKERPGLIVYGG---DDRIGALTKKVEHNTKFKIGNLNVQCLFTPCHTTGHIC 621
GGN +L KER G V G DRI + ++ + K+ V+ L TP HT GHI
Sbjct: 137 IGGNAEL-KERYGAKVIGSAVDKDRIPGIDILLKDSDKWMFAGHEVRILDTPGHTQGHIS 195
Query: 622 YFVTAPEEGNDSVVFTGDTLFLGGCG 699
++ + +FTGD ++ CG
Sbjct: 196 FYFP-----GSATIFTGDLIYSLSCG 216
>At1g53580.2 68414.m06085 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
GI:1644427 from (Arabidopsis thaliana)
Length = 256
Score = 49.2 bits (112), Expect = 3e-06
Identities = 38/137 (27%), Positives = 60/137 (43%), Gaps = 6/137 (4%)
Frame = +1
Query: 310 YMYLIVDKA--TKEAAIVDPVEPKTV---LKAVEEQGVNLXXXXXXXXXXXXAGGNEDLI 474
+ YL+ D + K A ++DPV+ KTV LK ++E G+ L G L
Sbjct: 27 FTYLLADVSHPDKPALLIDPVD-KTVDRDLKLIDELGLKLIYAMNTHVHADHVTGTGLLK 85
Query: 475 KERPGLIVYGGDDRIGALTKKVEHNTKFKIGNLNVQCLFTPCHTTGHICYFV-TAPEEGN 651
+ PG+ +E K IG++ ++ TP HT G + Y ++
Sbjct: 86 TKLPGVKSVISKASGSKADLFLEPGDKVSIGDIYLEVRATPGHTAGCVTYVTGEGADQPQ 145
Query: 652 DSVVFTGDTLFLGGCGR 702
+ FTGD + + GCGR
Sbjct: 146 PRMAFTGDAVLIRGCGR 162
>At1g53580.1 68414.m06084 hydroxyacylglutathione hydrolase, putative
/ glyoxalase II, putative similar to glyoxalase II
GI:1644427 from (Arabidopsis thaliana)
Length = 256
Score = 49.2 bits (112), Expect = 3e-06
Identities = 38/137 (27%), Positives = 60/137 (43%), Gaps = 6/137 (4%)
Frame = +1
Query: 310 YMYLIVDKA--TKEAAIVDPVEPKTV---LKAVEEQGVNLXXXXXXXXXXXXAGGNEDLI 474
+ YL+ D + K A ++DPV+ KTV LK ++E G+ L G L
Sbjct: 27 FTYLLADVSHPDKPALLIDPVD-KTVDRDLKLIDELGLKLIYAMNTHVHADHVTGTGLLK 85
Query: 475 KERPGLIVYGGDDRIGALTKKVEHNTKFKIGNLNVQCLFTPCHTTGHICYFV-TAPEEGN 651
+ PG+ +E K IG++ ++ TP HT G + Y ++
Sbjct: 86 TKLPGVKSVISKASGSKADLFLEPGDKVSIGDIYLEVRATPGHTAGCVTYVTGEGADQPQ 145
Query: 652 DSVVFTGDTLFLGGCGR 702
+ FTGD + + GCGR
Sbjct: 146 PRMAFTGDAVLIRGCGR 162
>At3g59550.1 68416.m06646 cohesion family protein SYN3 (SYN3) nearly
identical to cohesion family protein SYN3 [Arabidopsis
thaliana] GI:12006362; supporting cDNA
gi|12006361|gb|AF281155.1|AF281155
Length = 693
Score = 27.9 bits (59), Expect = 7.1
Identities = 11/48 (22%), Positives = 29/48 (60%), Gaps = 1/48 (2%)
Frame = +1
Query: 160 VNSLPSGLSQRIT-KLYFRALIRNQREAHSSQEDHQYKNMDVKILPAL 300
++ + +G ++++ +++F L+ R Q+D Y ++ +K++PAL
Sbjct: 640 LSEILAGKTRKLAARMFFETLVLKSRGLIDMQQDRPYGDIALKLMPAL 687
>At5g27700.1 68418.m03322 40S ribosomal protein S21 (RPS21C)
ribosomal protein S21, Zea mays, PIR:T03945
Length = 85
Score = 27.5 bits (58), Expect = 9.4
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = +1
Query: 526 LTKKVEHNTKFKIGNLNVQCLFTPCHTTGHICYFVTAPEEGNDSV 660
+T K + + IG+L+ L+T TT +C FV A + + V
Sbjct: 24 ITSKDHASVQLNIGHLDANGLYTGQFTTFALCGFVRAQGDADSGV 68
>At3g53890.1 68416.m05953 40S ribosomal protein S21 (RPS21B)
ribosomal protein S21, cytosolic - Oryza sativa,
PIR:S38357
Length = 82
Score = 27.5 bits (58), Expect = 9.4
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = +1
Query: 526 LTKKVEHNTKFKIGNLNVQCLFTPCHTTGHICYFVTAPEEGNDSV 660
+T K + + IG+L+ L+T TT +C FV A + + V
Sbjct: 24 ITSKDHASVQLNIGHLDANGLYTGQFTTFALCGFVRAQGDADSGV 68
>At2g25730.1 68415.m03084 expressed protein
Length = 2464
Score = 27.5 bits (58), Expect = 9.4
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
Frame = +1
Query: 235 EAHSSQEDHQYKNMDVKILPALQDNYMYL-IVDKATKEAAIVDPVEPKTVLKA 390
EA ++ QY ++ +K PAL+DN + + KA I P EP+ + A
Sbjct: 1590 EALLMRKQLQYASLILKEFPALRDNNVIMAYAAKAISVTIIPPPREPRITVSA 1642
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,983,419
Number of Sequences: 28952
Number of extensions: 270762
Number of successful extensions: 593
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 572
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 581
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1555552968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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