BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0165 (750 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosacch... 30 0.40 SPBC29A10.06c |||conserved fungal protein|Schizosaccharomyces po... 28 1.6 SPCC1739.12 |ppe1|esp1, ppx1|serine/threonine protein phosphatas... 27 2.9 SPBC21H7.05 |sfc6||transcription factor TFIIIC complex subunit S... 27 3.8 SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos... 27 3.8 SPAC25B8.06c |||serine-tRNA ligase|Schizosaccharomyces pombe|chr... 26 5.0 >SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosaccharomyces pombe|chr 2|||Manual Length = 269 Score = 29.9 bits (64), Expect = 0.40 Identities = 15/30 (50%), Positives = 17/30 (56%), Gaps = 7/30 (23%) Frame = +1 Query: 13 PCGHVVACAKCALS-------TDKCPMCRR 81 PCGH+ C C LS T KCP+CRR Sbjct: 220 PCGHIF-CNFCILSALGTTAATQKCPVCRR 248 >SPBC29A10.06c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 295 Score = 27.9 bits (59), Expect = 1.6 Identities = 23/71 (32%), Positives = 35/71 (49%) Frame = +3 Query: 327 DVEMILFII*NSNDKV*VNKCTYYFRLSICPTDKSEMYIGVFISLQQSKTFFLNLRIYIY 506 D++ + II NSN +C YY+ L +KSE Y +SL ++ ++ Y Y Sbjct: 68 DLQSLFMIIENSNIDELKQQCFYYYLLK--DWEKSETY-SEQVSLPKNYQHLMD--GYYY 122 Query: 507 TYKLKF*RDIN 539 +LKF IN Sbjct: 123 LDRLKFEEAIN 133 >SPCC1739.12 |ppe1|esp1, ppx1|serine/threonine protein phosphatase Ppe1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 305 Score = 27.1 bits (57), Expect = 2.9 Identities = 10/31 (32%), Positives = 15/31 (48%) Frame = -3 Query: 112 RSRAAPHS*TSCDTSGTCPSTARTWRTPPRG 20 R++ PH + CD + P +W PRG Sbjct: 179 RAQEIPHEGSFCDLMWSDPEDIESWTVSPRG 209 >SPBC21H7.05 |sfc6||transcription factor TFIIIC complex subunit Sfc6|Schizosaccharomyces pombe|chr 2|||Manual Length = 582 Score = 26.6 bits (56), Expect = 3.8 Identities = 10/20 (50%), Positives = 14/20 (70%) Frame = +2 Query: 398 F*IINLSHRQK*NVHRCFHI 457 F ++ SH+QK NVHR F + Sbjct: 492 FRLLGFSHKQKANVHRIFQL 511 >SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 26.6 bits (56), Expect = 3.8 Identities = 12/32 (37%), Positives = 20/32 (62%), Gaps = 4/32 (12%) Frame = +1 Query: 10 VPCGHVV--ACAKCAL--STDKCPMCRRTFTN 93 +PC H+ AC L ++D CP+C+++ TN Sbjct: 515 LPCHHIFHPACIDPYLLKNSDLCPLCKQSVTN 546 >SPAC25B8.06c |||serine-tRNA ligase|Schizosaccharomyces pombe|chr 1|||Manual Length = 454 Score = 26.2 bits (55), Expect = 5.0 Identities = 24/81 (29%), Positives = 36/81 (44%), Gaps = 4/81 (4%) Frame = -1 Query: 315 LQITNSFLHVNSRVLTSSYTLPHRYQFVNLFNVKITGYAVSF---EGWFQHASGPPGEV- 148 LQI N +++ S ++ + + L + IT YA+ F +GW PP V Sbjct: 165 LQIANEGINLEDAAQASGHSFCYTTGDIALLEMAITNYAMDFAISKGWCPVI--PPTIVR 222 Query: 147 TSIQLARRVLSREVEPHRIRE 85 T I LA R+ E +I E Sbjct: 223 TDIALACGFQPRDEEGQQIYE 243 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,885,287 Number of Sequences: 5004 Number of extensions: 57424 Number of successful extensions: 134 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 129 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 134 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 357280532 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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