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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0165
         (750 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosacch...    30   0.40 
SPBC29A10.06c |||conserved fungal protein|Schizosaccharomyces po...    28   1.6  
SPCC1739.12 |ppe1|esp1, ppx1|serine/threonine protein phosphatas...    27   2.9  
SPBC21H7.05 |sfc6||transcription factor TFIIIC complex subunit S...    27   3.8  
SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos...    27   3.8  
SPAC25B8.06c |||serine-tRNA ligase|Schizosaccharomyces pombe|chr...    26   5.0  

>SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 269

 Score = 29.9 bits (64), Expect = 0.40
 Identities = 15/30 (50%), Positives = 17/30 (56%), Gaps = 7/30 (23%)
 Frame = +1

Query: 13  PCGHVVACAKCALS-------TDKCPMCRR 81
           PCGH+  C  C LS       T KCP+CRR
Sbjct: 220 PCGHIF-CNFCILSALGTTAATQKCPVCRR 248


>SPBC29A10.06c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 295

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 23/71 (32%), Positives = 35/71 (49%)
 Frame = +3

Query: 327 DVEMILFII*NSNDKV*VNKCTYYFRLSICPTDKSEMYIGVFISLQQSKTFFLNLRIYIY 506
           D++ +  II NSN      +C YY+ L     +KSE Y    +SL ++    ++   Y Y
Sbjct: 68  DLQSLFMIIENSNIDELKQQCFYYYLLK--DWEKSETY-SEQVSLPKNYQHLMD--GYYY 122

Query: 507 TYKLKF*RDIN 539
             +LKF   IN
Sbjct: 123 LDRLKFEEAIN 133


>SPCC1739.12 |ppe1|esp1, ppx1|serine/threonine protein phosphatase
           Ppe1 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 305

 Score = 27.1 bits (57), Expect = 2.9
 Identities = 10/31 (32%), Positives = 15/31 (48%)
 Frame = -3

Query: 112 RSRAAPHS*TSCDTSGTCPSTARTWRTPPRG 20
           R++  PH  + CD   + P    +W   PRG
Sbjct: 179 RAQEIPHEGSFCDLMWSDPEDIESWTVSPRG 209


>SPBC21H7.05 |sfc6||transcription factor TFIIIC complex subunit
           Sfc6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 582

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 10/20 (50%), Positives = 14/20 (70%)
 Frame = +2

Query: 398 F*IINLSHRQK*NVHRCFHI 457
           F ++  SH+QK NVHR F +
Sbjct: 492 FRLLGFSHKQKANVHRIFQL 511


>SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 556

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 12/32 (37%), Positives = 20/32 (62%), Gaps = 4/32 (12%)
 Frame = +1

Query: 10  VPCGHVV--ACAKCAL--STDKCPMCRRTFTN 93
           +PC H+   AC    L  ++D CP+C+++ TN
Sbjct: 515 LPCHHIFHPACIDPYLLKNSDLCPLCKQSVTN 546


>SPAC25B8.06c |||serine-tRNA ligase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 454

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 24/81 (29%), Positives = 36/81 (44%), Gaps = 4/81 (4%)
 Frame = -1

Query: 315 LQITNSFLHVNSRVLTSSYTLPHRYQFVNLFNVKITGYAVSF---EGWFQHASGPPGEV- 148
           LQI N  +++      S ++  +    + L  + IT YA+ F   +GW      PP  V 
Sbjct: 165 LQIANEGINLEDAAQASGHSFCYTTGDIALLEMAITNYAMDFAISKGWCPVI--PPTIVR 222

Query: 147 TSIQLARRVLSREVEPHRIRE 85
           T I LA     R+ E  +I E
Sbjct: 223 TDIALACGFQPRDEEGQQIYE 243


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,885,287
Number of Sequences: 5004
Number of extensions: 57424
Number of successful extensions: 134
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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