Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0163
(354 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g01590.1 68418.m00074 expressed protein 29 0.67
At1g17145.1 68414.m02090 expressed protein 29 0.67
At5g08370.1 68418.m00986 alpha-galactosidase, putative / melibia... 29 1.2
At1g78420.1 68414.m09138 expressed protein 29 1.2
At1g69260.1 68414.m07939 expressed protein 28 2.1
At2g26480.1 68415.m03177 UDP-glucoronosyl/UDP-glucosyl transfera... 27 2.7
At5g37400.1 68418.m04499 hypothetical protein contains Pfam PF04... 27 4.7
At3g23980.1 68416.m03012 dentin sialophosphoprotein-related cont... 27 4.7
At1g28570.2 68414.m03518 GDSL-motif lipase, putative similar to ... 27 4.7
At1g28570.1 68414.m03517 GDSL-motif lipase, putative similar to ... 27 4.7
At5g66160.2 68418.m08334 protease-associated zinc finger (C3HC4-... 26 6.3
At5g66160.1 68418.m08335 protease-associated zinc finger (C3HC4-... 26 6.3
At5g55630.2 68418.m06937 outward rectifying potassium channel (K... 26 6.3
At5g55630.1 68418.m06936 outward rectifying potassium channel (K... 26 6.3
At5g45300.1 68418.m05561 glycosyl hydrolase family 14 protein si... 26 6.3
At4g34930.1 68417.m04952 1-phosphatidylinositol phosphodiesteras... 26 6.3
At5g54650.2 68418.m06805 formin homology 2 domain-containing pro... 26 8.3
At5g54650.1 68418.m06804 formin homology 2 domain-containing pro... 26 8.3
At5g15550.2 68418.m01821 transducin family protein / WD-40 repea... 26 8.3
At5g15550.1 68418.m01820 transducin family protein / WD-40 repea... 26 8.3
At3g19910.1 68416.m02521 zinc finger (C3HC4-type RING finger) fa... 26 8.3
>At5g01590.1 68418.m00074 expressed protein
Length = 424
Score = 29.5 bits (63), Expect = 0.67
Identities = 11/45 (24%), Positives = 21/45 (46%)
Frame = +3
Query: 99 WNATKSSSIANLMIGKCSLDDAMKEFRLNGLDSPYNPDEDNIWSA 233
W + ++S + M + D M + L D+PY ++ + W A
Sbjct: 118 WESFRASPVVQFMTRAEEIADDMNKMELEDNDTPYRKEDKDYWRA 162
>At1g17145.1 68414.m02090 expressed protein
Length = 335
Score = 29.5 bits (63), Expect = 0.67
Identities = 20/52 (38%), Positives = 26/52 (50%), Gaps = 4/52 (7%)
Frame = -1
Query: 297 GLATTPA----WSTCSIGSMYLQLLARSRCCPRRDCTENPSRSV*TPSSRHP 154
GL TP+ C I +Y L RSRCC + CTE R + +P+S P
Sbjct: 49 GLEETPSSHHDLEECPICFLYYPSLNRSRCCMKSICTECFLR-MKSPNSAQP 99
>At5g08370.1 68418.m00986 alpha-galactosidase, putative / melibiase,
putative / alpha-D-galactoside galactohydrolase,
putative similar to alpha-galactosidase SP:Q42656 from
[Coffea arabica]
Length = 396
Score = 28.7 bits (61), Expect = 1.2
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = +3
Query: 213 EDNIWSARAVADTSTQWNTYSRQVSWPAPE 302
+DN S +AD + +W +Y+R SW P+
Sbjct: 218 QDNWKSMTLIADQNDRWASYARPGSWNDPD 247
>At1g78420.1 68414.m09138 expressed protein
Length = 401
Score = 28.7 bits (61), Expect = 1.2
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = -1
Query: 267 CSIGSMYLQLLARSRCCPRRDCTE 196
C I +Y L RSRCC + CTE
Sbjct: 59 CPICFLYYPSLNRSRCCMKSICTE 82
>At1g69260.1 68414.m07939 expressed protein
Length = 345
Score = 27.9 bits (59), Expect = 2.1
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +1
Query: 265 TRTPGRCRGQPPSGLPRYRSRA 330
T T R RG+P SGLPR+ + A
Sbjct: 163 TATASRRRGRPSSGLPRWSATA 184
>At2g26480.1 68415.m03177 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 452
Score = 27.5 bits (58), Expect = 2.7
Identities = 19/66 (28%), Positives = 33/66 (50%), Gaps = 5/66 (7%)
Frame = -3
Query: 217 SSSGLYGESKPFSLNSFIASSREH-----LPIIRLAMLDDLVAFQSEDRFERSGISWLNE 53
SSSG+ S NSFI +++E P+ L M + ++ S ER+ + WL +
Sbjct: 197 SSSGIIHNSSDCLENSFITTAQEKWGVPVYPVGPLHMTNSAMSCPSLFEEERNCLEWLEK 256
Query: 52 DESTEL 35
E++ +
Sbjct: 257 QETSSV 262
>At5g37400.1 68418.m04499 hypothetical protein contains Pfam PF04510
: Family of unknown function (DUF577)); common family
comprised of At5g37410, At5g37400, At5g37920,
At5g37460, At5g37650, At5g37470, At5g37420, At5g37430
Length = 623
Score = 26.6 bits (56), Expect = 4.7
Identities = 14/25 (56%), Positives = 17/25 (68%), Gaps = 4/25 (16%)
Frame = +3
Query: 135 MIGKCSLD----DAMKEFRLNGLDS 197
++ KCSLD D +EF LNGLDS
Sbjct: 235 IVWKCSLDVVWNDMEEEFLLNGLDS 259
>At3g23980.1 68416.m03012 dentin sialophosphoprotein-related
contains weak similarity to Dentin sialophosphoprotein
precursor (Swiss-Prot:Q9NZW4) [Homo sapiens]
Length = 736
Score = 26.6 bits (56), Expect = 4.7
Identities = 11/34 (32%), Positives = 17/34 (50%)
Frame = +3
Query: 72 PLRSNLSSDWNATKSSSIANLMIGKCSLDDAMKE 173
PL+ + S N T + + NL ++DD KE
Sbjct: 63 PLKQSAESTSNETHTKDVYNLSFSNTAMDDGSKE 96
>At1g28570.2 68414.m03518 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains
InterPro Entry IPR001087 Lipolytic enzyme, G-D-S-L
family
Length = 317
Score = 26.6 bits (56), Expect = 4.7
Identities = 14/53 (26%), Positives = 24/53 (45%)
Frame = -1
Query: 297 GLATTPAWSTCSIGSMYLQLLARSRCCPRRDCTENPSRSV*TPSSRHPESIYR 139
G + P + C++G + L R R +C ++PS+ V E+ YR
Sbjct: 230 GFISRPLPACCALGGPFNFTLGRKRGTQVPECCDDPSKYVSWDGVHMTEAAYR 282
>At1g28570.1 68414.m03517 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains
InterPro Entry IPR001087 Lipolytic enzyme, G-D-S-L
family
Length = 384
Score = 26.6 bits (56), Expect = 4.7
Identities = 14/53 (26%), Positives = 24/53 (45%)
Frame = -1
Query: 297 GLATTPAWSTCSIGSMYLQLLARSRCCPRRDCTENPSRSV*TPSSRHPESIYR 139
G + P + C++G + L R R +C ++PS+ V E+ YR
Sbjct: 297 GFISRPLPACCALGGPFNFTLGRKRGTQVPECCDDPSKYVSWDGVHMTEAAYR 349
>At5g66160.2 68418.m08334 protease-associated zinc finger
(C3HC4-type RING finger) family protein contains Pfam
domain, PF02225: protease-associated (PA) domain and
Pfam domain, PF00097: Zinc finger, C3HC4 type (RING
finger); similar to ReMembR-H2 protein JR702
[Arabidopsis thaliana] gi|6942149|gb|AAF32326; identical
to cDNA ReMembR-H2 protein JR700 mRNA, complete cds
GI:6942146
Length = 290
Score = 26.2 bits (55), Expect = 6.3
Identities = 10/42 (23%), Positives = 23/42 (54%)
Frame = +3
Query: 72 PLRSNLSSDWNATKSSSIANLMIGKCSLDDAMKEFRLNGLDS 197
PL +S+W +++ A ++ G+CS +D + + +G +
Sbjct: 67 PLLHAAASNWTQHRTTKFALIIRGECSFEDKLLNAQNSGFQA 108
>At5g66160.1 68418.m08335 protease-associated zinc finger
(C3HC4-type RING finger) family protein contains Pfam
domain, PF02225: protease-associated (PA) domain and
Pfam domain, PF00097: Zinc finger, C3HC4 type (RING
finger); similar to ReMembR-H2 protein JR702
[Arabidopsis thaliana] gi|6942149|gb|AAF32326; identical
to cDNA ReMembR-H2 protein JR700 mRNA, complete cds
GI:6942146
Length = 310
Score = 26.2 bits (55), Expect = 6.3
Identities = 10/42 (23%), Positives = 23/42 (54%)
Frame = +3
Query: 72 PLRSNLSSDWNATKSSSIANLMIGKCSLDDAMKEFRLNGLDS 197
PL +S+W +++ A ++ G+CS +D + + +G +
Sbjct: 67 PLLHAAASNWTQHRTTKFALIIRGECSFEDKLLNAQNSGFQA 108
>At5g55630.2 68418.m06937 outward rectifying potassium channel
(KCO1) identical to kco1 [Arabidopsis thaliana]
gi|2230761|emb|CAA69158 of the 2 pore, 4 transmembrane
(2P/4TM) K+ channel family, PMID:11500563
Length = 363
Score = 26.2 bits (55), Expect = 6.3
Identities = 15/36 (41%), Positives = 16/36 (44%)
Frame = -1
Query: 237 LARSRCCPRRDCTENPSRSV*TPSSRHPESIYRSSD 130
L RSR PR DC N + P HP I SD
Sbjct: 37 LRRSRSAPRGDCMYNDDVKIDEPPP-HPSKIPMFSD 71
>At5g55630.1 68418.m06936 outward rectifying potassium channel
(KCO1) identical to kco1 [Arabidopsis thaliana]
gi|2230761|emb|CAA69158 of the 2 pore, 4 transmembrane
(2P/4TM) K+ channel family, PMID:11500563
Length = 363
Score = 26.2 bits (55), Expect = 6.3
Identities = 15/36 (41%), Positives = 16/36 (44%)
Frame = -1
Query: 237 LARSRCCPRRDCTENPSRSV*TPSSRHPESIYRSSD 130
L RSR PR DC N + P HP I SD
Sbjct: 37 LRRSRSAPRGDCMYNDDVKIDEPPP-HPSKIPMFSD 71
>At5g45300.1 68418.m05561 glycosyl hydrolase family 14 protein
similar to beta-amylase GI:13560977 from [Castanea
crenata]
Length = 689
Score = 26.2 bits (55), Expect = 6.3
Identities = 15/46 (32%), Positives = 24/46 (52%)
Frame = +1
Query: 217 TTSGAREQLQIHRPNGTRTPGRCRGQPPSGLPRYRSRALTALAV*P 354
TT+G+++ P+ + P R R QP S PR + A A ++ P
Sbjct: 10 TTTGSQDPNLDPIPDPDQFPNRNRNQPQSRRPRGFAAAAAAASIAP 55
>At4g34930.1 68417.m04952 1-phosphatidylinositol
phosphodiesterase-related contains weak similarity to
1-phosphatidylinositol phosphodiesterase precursor (EC
4.6.1.13) (Phosphatidylinositol diacylglycerol-lyase)
(Phosphatidylinositol- specific phospholipase C)
(PI-PLC). (Swiss-Prot:P34024) [Listeria monocytogenes]
Length = 391
Score = 26.2 bits (55), Expect = 6.3
Identities = 15/46 (32%), Positives = 24/46 (52%), Gaps = 3/46 (6%)
Frame = -2
Query: 272 VRVPLGRCICN---CSRAPDVVLVGIVRRIQAVQSELLHRVIQRAF 144
+R RC+C+ S DVVL ++R + QSE++ I+ F
Sbjct: 170 IRFQEDRCVCHGALSSYNVDVVLNDVIRFVSETQSEIIILEIRTEF 215
>At5g54650.2 68418.m06805 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 900
Score = 25.8 bits (54), Expect = 8.3
Identities = 9/29 (31%), Positives = 19/29 (65%)
Frame = +3
Query: 204 NPDEDNIWSARAVADTSTQWNTYSRQVSW 290
NP++D I+ ++ +A ++ Q N + + SW
Sbjct: 35 NPEKDEIFLSQFMAPSTGQVNEHMEETSW 63
>At5g54650.1 68418.m06804 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 900
Score = 25.8 bits (54), Expect = 8.3
Identities = 9/29 (31%), Positives = 19/29 (65%)
Frame = +3
Query: 204 NPDEDNIWSARAVADTSTQWNTYSRQVSW 290
NP++D I+ ++ +A ++ Q N + + SW
Sbjct: 35 NPEKDEIFLSQFMAPSTGQVNEHMEETSW 63
>At5g15550.2 68418.m01821 transducin family protein / WD-40 repeat
family protein similar to YTM1 - Homo sapiens,
EMBL:AF242546; contains Pfam PF00400: WD domain, G-beta
repeat (7 copies,1 weak);
Length = 402
Score = 25.8 bits (54), Expect = 8.3
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = -1
Query: 183 SV*TPSSRHPESIYRSSDWQCWMTWWHSNRKTDLNE 76
SV + S++ ++ SS W C + W++N T E
Sbjct: 207 SVQSVSAQKSGNMVCSSSWDCTINLWNTNESTSEGE 242
>At5g15550.1 68418.m01820 transducin family protein / WD-40 repeat
family protein similar to YTM1 - Homo sapiens,
EMBL:AF242546; contains Pfam PF00400: WD domain, G-beta
repeat (7 copies,1 weak);
Length = 433
Score = 25.8 bits (54), Expect = 8.3
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = -1
Query: 183 SV*TPSSRHPESIYRSSDWQCWMTWWHSNRKTDLNE 76
SV + S++ ++ SS W C + W++N T E
Sbjct: 207 SVQSVSAQKSGNMVCSSSWDCTINLWNTNESTSEGE 242
>At3g19910.1 68416.m02521 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 340
Score = 25.8 bits (54), Expect = 8.3
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = +2
Query: 17 SDYGKWELGGFVF 55
SDYG WE G +V+
Sbjct: 121 SDYGSWETGSYVY 133
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,779,539
Number of Sequences: 28952
Number of extensions: 185063
Number of successful extensions: 647
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 636
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 646
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 449370720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -