Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0161
(746 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g23930.1 68416.m03006 expressed protein 33 0.20
At3g51010.1 68416.m05585 expressed protein 33 0.27
At5g52280.1 68418.m06488 protein transport protein-related low s... 32 0.35
At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains... 31 0.61
At5g45520.1 68418.m05591 hypothetical protein 30 1.9
At5g25070.1 68418.m02971 expressed protein 30 1.9
At2g25350.1 68415.m03032 phox (PX) domain-containing protein wea... 30 1.9
At1g48280.1 68414.m05393 hydroxyproline-rich glycoprotein family... 30 1.9
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 29 2.5
At1g63300.1 68414.m07156 expressed protein similar to Intracellu... 29 2.5
At5g08080.1 68418.m00942 syntaxin, putative (SYP132) similar to ... 29 3.3
At5g05210.1 68418.m00555 nucleolar matrix protein-related contai... 29 3.3
At5g03660.1 68418.m00325 expressed protein low similarity to out... 29 3.3
At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR... 29 4.3
At3g07780.1 68416.m00949 expressed protein 29 4.3
At1g01670.1 68414.m00085 U-box domain-containing protein 29 4.3
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 28 7.6
At3g43160.1 68416.m04554 expressed protein merozoite surface pro... 28 7.6
At1g30650.1 68414.m03748 WRKY family transcription factor contai... 28 7.6
At1g21630.1 68414.m02708 calcium-binding EF hand family protein ... 28 7.6
At1g05320.1 68414.m00539 myosin-related similar to non-muscle my... 28 7.6
At2g18540.1 68415.m02160 cupin family protein contains Pfam prof... 27 10.0
>At3g23930.1 68416.m03006 expressed protein
Length = 224
Score = 33.1 bits (72), Expect = 0.20
Identities = 15/40 (37%), Positives = 21/40 (52%)
Frame = +1
Query: 178 KPAPKQEGEGDPEFIKRQDQKRSDLDEQLKEYINEWRKQR 297
K K + G IK +D++ + QLKE EWRK+R
Sbjct: 17 KETSKDQSRGRRHLIKERDEREKVMFLQLKEAEREWRKER 56
>At3g51010.1 68416.m05585 expressed protein
Length = 188
Score = 32.7 bits (71), Expect = 0.27
Identities = 22/66 (33%), Positives = 34/66 (51%)
Frame = +1
Query: 448 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEE 627
I ++R + +KKRQA++Q K + +++K A ER +QLEEE
Sbjct: 125 IGQRRAFILSEKKKRQALVQEAKRKKRIKQ---VERKMAAVARDRAWAERLIELQQLEEE 181
Query: 628 KKISLS 645
KK S+S
Sbjct: 182 KKKSMS 187
>At5g52280.1 68418.m06488 protein transport protein-related low
similarity to SP|P25386 Intracellular protein transport
protein USO1 {Saccharomyces cerevisiae}
Length = 853
Score = 32.3 bits (70), Expect = 0.35
Identities = 26/89 (29%), Positives = 41/89 (46%), Gaps = 3/89 (3%)
Frame = +1
Query: 448 IEEKRQRLE-EAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLE- 621
++EK +RL E E K K N +Q K+ E + KEQ +
Sbjct: 583 LQEKCKRLSLEMESKLSEHENLTKKTLAEANNLRLQNKTLEEMQEKTHTEITQEKEQRKH 642
Query: 622 -EEKKISLSIRIKPLTIEGLSVDKLRQKA 705
EEK +LS++++ L E L + KLR ++
Sbjct: 643 VEEKNKALSMKVQMLESEVLKLTKLRDES 671
>At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains
ATP/GTP-binding site motif A (P-loop), PROSITE:PS00017
Length = 1340
Score = 31.5 bits (68), Expect = 0.61
Identities = 22/89 (24%), Positives = 44/89 (49%), Gaps = 1/89 (1%)
Frame = +1
Query: 448 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNA-QLERNKTKEQLEE 624
++ K++ KK++ + K +KTG + +KK+ + +S +++RN T E+ +
Sbjct: 898 VDVKQETGSPDTKKKEGASSSSKKDTKTGEDKKAEKKNNSETMSEGKKIDRNNTDEKEVK 957
Query: 625 EKKISLSIRIKPLTIEGLSVDKLRQKAQE 711
EK I+ + E K R+K +E
Sbjct: 958 EKVTEKEIKERGGKDESRIQVKDRKKCEE 986
>At5g45520.1 68418.m05591 hypothetical protein
Length = 1167
Score = 29.9 bits (64), Expect = 1.9
Identities = 19/68 (27%), Positives = 36/68 (52%), Gaps = 5/68 (7%)
Frame = +1
Query: 445 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENF-----GLSNAQLERNKTK 609
++E+ ++ E E++ + +++ KT P+ +K +N G SN + + +K K
Sbjct: 624 NLEDGKKHDEGKEERSLKSDEVVEEEKKTSPSEEATEKFQNKPGDQKGKSNVEGDGDKGK 683
Query: 610 EQLEEEKK 633
LEEEKK
Sbjct: 684 ADLEEEKK 691
>At5g25070.1 68418.m02971 expressed protein
Length = 736
Score = 29.9 bits (64), Expect = 1.9
Identities = 27/85 (31%), Positives = 41/85 (48%), Gaps = 1/85 (1%)
Frame = +1
Query: 454 EKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEE-EK 630
EKR EAEKK A + K+A + + KS N Q+E K +LE+ E
Sbjct: 565 EKRMPELEAEKKVAASTRNFKEAGR----IAAEAKSLNLEKDKTQMETGKANAELEKAEH 620
Query: 631 KISLSIRIKPLTIEGLSVDKLRQKA 705
+I +I+ + IE L + K ++ A
Sbjct: 621 EIEETIK-RLQEIEKLILSKEKELA 644
>At2g25350.1 68415.m03032 phox (PX) domain-containing protein weak
similarity to SP|Q9UTK5 Abnormal long morphology protein
1 (Sp8) {Schizosaccharomyces pombe}; contains Pfam
profile PF00787: PX domain
Length = 643
Score = 29.9 bits (64), Expect = 1.9
Identities = 30/103 (29%), Positives = 48/103 (46%), Gaps = 3/103 (2%)
Frame = +1
Query: 445 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEE 624
D+EE RQ+ E E K ++ D SKT N TI + E E + TK+QLE+
Sbjct: 455 DMEELRQKTFEMELKLKSKEDGSSD-SKTSGNSTISESHE------LLQEMDATKQQLED 507
Query: 625 --EKKISLSIRIK-PLTIEGLSVDKLRQKAQELWECIVKLETE 744
+ + L + K + + V LR+ E+ + + + TE
Sbjct: 508 LSRRYVELEAKSKADIKVLVREVKSLRRSHMEMEKELTRSLTE 550
>At1g48280.1 68414.m05393 hydroxyproline-rich glycoprotein family
protein
Length = 558
Score = 29.9 bits (64), Expect = 1.9
Identities = 26/74 (35%), Positives = 37/74 (50%), Gaps = 4/74 (5%)
Frame = +1
Query: 451 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTK---EQLE 621
+EKR+R+EE E+K +KD N ++ + E SN +LE N K + +
Sbjct: 112 DEKRKRMEELEEKLVVNESLIKDLQLQVLN--LKTELEEARNSNVELELNNRKLSQDLVS 169
Query: 622 EEKKI-SLSIRIKP 660
E KI SLS KP
Sbjct: 170 AEAKISSLSSNDKP 183
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 29.5 bits (63), Expect = 2.5
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +1
Query: 187 PKQEGEGDPEFIKRQDQKRSDLDEQLKEYINEW 285
PK+E EG+ E + + +K +++KE +EW
Sbjct: 232 PKKENEGEVEEVDEEKEKDGKKKKKIKEVSHEW 264
>At1g63300.1 68414.m07156 expressed protein similar to Intracellular
protein transport protein USO1 (Swiss-Prot:P25386)
[Saccharomyces cerevisiae]; similar to Myosin II heavy
chain, non muscle (Swiss-Prot:P08799) [Dictyostelium
discoideum]
Length = 1029
Score = 29.5 bits (63), Expect = 2.5
Identities = 25/99 (25%), Positives = 44/99 (44%)
Frame = +1
Query: 448 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEE 627
+ E ++L + + ML+ + + S N QK+ E +N E KE++E
Sbjct: 684 LHELSEKLSFKTSQMERMLENLDEKSNEIDN---QKRHEEDVTANLNQEIKILKEEIENL 740
Query: 628 KKISLSIRIKPLTIEGLSVDKLRQKAQELWECIVKLETE 744
KK S+ ++ E L VD L + + + E L+ E
Sbjct: 741 KKNQDSLMLQAEQAENLRVD-LEKTKKSVMEAEASLQRE 778
>At5g08080.1 68418.m00942 syntaxin, putative (SYP132) similar to
SP|Q9ZSD4 Syntaxin 121 (AtSYP121) (Syntaxin-related
protein At-Syr1) {Arabidopsis thaliana}
Length = 304
Score = 29.1 bits (62), Expect = 3.3
Identities = 24/83 (28%), Positives = 40/83 (48%)
Frame = +1
Query: 448 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEE 627
I+++ +L++ KK QA + K +K I+K E + R K +LEE
Sbjct: 44 IDKQYDKLDKLLKKLQASHEESKSVTKAPAMKAIKKTMEKDVDEVGSIARF-IKGKLEEL 102
Query: 628 KKISLSIRIKPLTIEGLSVDKLR 696
+ +L+ R KP +G VD+ R
Sbjct: 103 DRENLANRQKPGCAKGSGVDRSR 125
>At5g05210.1 68418.m00555 nucleolar matrix protein-related contains
Pfam domain, PF04935: Surfeit locus protein 6
Length = 386
Score = 29.1 bits (62), Expect = 3.3
Identities = 12/59 (20%), Positives = 33/59 (55%)
Frame = +1
Query: 460 RQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEKKI 636
+Q + + +K+++ + K+ + F ++K+ + G ++E+NK ++ + EKK+
Sbjct: 310 KQSIHKDKKRQEKNAEKWKERIEGQQKFKVEKQQKRSGNIADRIEQNKMRKIAKREKKL 368
>At5g03660.1 68418.m00325 expressed protein low similarity to outer
surface protein F [Borrelia burgdorferi] GI:466482;
contains Pfam profile PF04949: Family of unknown
function (DUF662)
Length = 173
Score = 29.1 bits (62), Expect = 3.3
Identities = 16/49 (32%), Positives = 26/49 (53%)
Frame = +1
Query: 511 MKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEKKISLSIRIK 657
+KD +F+ Q K E+ LS L K KE+ E+KK+ + R++
Sbjct: 18 LKDQLSESMSFSSQMKKEDDELSMKALSAFKAKEEEIEKKKMEIRERVQ 66
>At5g41540.1 68418.m05048 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1038
Score = 28.7 bits (61), Expect = 4.3
Identities = 19/50 (38%), Positives = 24/50 (48%)
Frame = +1
Query: 445 DIEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLE 594
D EKRQ L EAE+ R + S G +F + K SE F +S E
Sbjct: 502 DEPEKRQFLVEAEEIRDVLANETGTGSVLGISFDMSKVSE-FSISGRAFE 550
>At3g07780.1 68416.m00949 expressed protein
Length = 566
Score = 28.7 bits (61), Expect = 4.3
Identities = 20/59 (33%), Positives = 35/59 (59%), Gaps = 2/59 (3%)
Frame = +1
Query: 574 LSNAQLERNKTKEQLEEEKKISLSIRIKPLTIE--GLSVDKLRQKAQELWECIVKLETE 744
++ Q+ER K K+Q+EE ++I +R+K E L ++ + +A+ L E IVK + E
Sbjct: 434 VAELQMERQKKKQQIEEVERI---VRLKQAEAEMFQLKANEAKVEAERL-ERIVKAKKE 488
>At1g01670.1 68414.m00085 U-box domain-containing protein
Length = 365
Score = 28.7 bits (61), Expect = 4.3
Identities = 19/73 (26%), Positives = 36/73 (49%), Gaps = 6/73 (8%)
Frame = +1
Query: 451 EEKRQRLEEAEKKRQ-----AMLQAMKDASKTGPNFT-IQKKSENFGLSNAQLERNKTKE 612
EE+R+RLE E KR+ M + ++A + T I E + E N+ K
Sbjct: 177 EEQRRRLEIEELKREKEQRDKMRRVREEALSSSSGVTKILYNEEVMRRREVEAELNRAKA 236
Query: 613 QLEEEKKISLSIR 651
++E+ K++ + ++
Sbjct: 237 EIEDMKRVQIELK 249
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 27.9 bits (59), Expect = 7.6
Identities = 20/71 (28%), Positives = 33/71 (46%), Gaps = 2/71 (2%)
Frame = +1
Query: 472 EEAEKKRQAMLQAMKDASKTGPNFT--IQKKSENFGLSNAQLERNKTKEQLEEEKKISLS 645
EEA+K R+ L+ KDAS + + KK+E + +KE E+ ++ +
Sbjct: 710 EEAKKTREQALRKRKDASFKHVIISEKVDKKAEKLQTKSLPYPYT-SKEVFEQSMRMPIG 768
Query: 646 IRIKPLTIEGL 678
P TI G+
Sbjct: 769 PEFNPTTIVGV 779
>At3g43160.1 68416.m04554 expressed protein merozoite surface
protein 2 (MSP-2), EMBL:PFU72951, Plasmodium falciparum
Length = 295
Score = 27.9 bits (59), Expect = 7.6
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +2
Query: 218 SSSVKTRSGRTWMNS*RNTSTNGANSGPRRRMSSNALKRSR 340
+S+ +TRSGR +++ +ST NS R SS K R
Sbjct: 103 NSTTRTRSGRGFIDYVLESSTGTENSSTRTAESSTRTKNGR 143
>At1g30650.1 68414.m03748 WRKY family transcription factor
contains Pfam profile: PF03106 WRKY DNA -binding domain
Length = 430
Score = 27.9 bits (59), Expect = 7.6
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = +1
Query: 4 SPRTPHSNVAVLPVQEPVPS 63
SP TP SN+ LP+ P PS
Sbjct: 30 SPETPPSNIWPLPLSHPTPS 49
>At1g21630.1 68414.m02708 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain; ESTs gb|T44428 and gb|AA395440 come from this
gene
Length = 1218
Score = 27.9 bits (59), Expect = 7.6
Identities = 17/49 (34%), Positives = 28/49 (57%)
Frame = +1
Query: 592 ERNKTKEQLEEEKKISLSIRIKPLTIEGLSVDKLRQKAQELWECIVKLE 738
E ++ EE+ K S ++ K LTIE + +++K EL++ IVK E
Sbjct: 656 ELESLAKKYEEKYKKSGNVGSK-LTIEEATFRDIQEKKMELYQAIVKFE 703
>At1g05320.1 68414.m00539 myosin-related similar to non-muscle
myosin II heavy chain (GI:19879404) [Loligo pealei];
ESTs gb|AA042402,gb|ATTS1380 come from this gene
Length = 828
Score = 27.9 bits (59), Expect = 7.6
Identities = 18/68 (26%), Positives = 35/68 (51%), Gaps = 3/68 (4%)
Frame = +1
Query: 448 IEEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKT---KEQL 618
+E+K + LEE +K + A+ A + G +IQ + +S + N++ +L
Sbjct: 155 LEQKLKSLEELSEKVSELKSALIVAEEEGKKSSIQMQEYQEKVSKLESSLNQSSARNSEL 214
Query: 619 EEEKKISL 642
EE+ +I+L
Sbjct: 215 EEDLRIAL 222
>At2g18540.1 68415.m02160 cupin family protein contains Pfam profile
PF00190: Cupin
Length = 707
Score = 27.5 bits (58), Expect = 10.0
Identities = 19/64 (29%), Positives = 34/64 (53%), Gaps = 4/64 (6%)
Frame = +1
Query: 454 EKRQRLEE-AEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQL---E 621
E+R++ EE A K+ +A + ++A + T +KK E + ER + +E+ E
Sbjct: 444 ERRRKEEEEARKREEAKRREEEEAKRREEEETERKKREEEEARKREEERKREEEEAKRRE 503
Query: 622 EEKK 633
EE+K
Sbjct: 504 EERK 507
Score = 27.5 bits (58), Expect = 10.0
Identities = 11/61 (18%), Positives = 32/61 (52%)
Frame = +1
Query: 451 EEKRQRLEEAEKKRQAMLQAMKDASKTGPNFTIQKKSENFGLSNAQLERNKTKEQLEEEK 630
EE+++ EEA+++ + + ++A + ++K E + + K +E++E ++
Sbjct: 490 EERKREEEEAKRREEERKKREEEAEQARKREEEREKEEEMAKKREEERQRKEREEVERKR 549
Query: 631 K 633
+
Sbjct: 550 R 550
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.315 0.130 0.365
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,609,521
Number of Sequences: 28952
Number of extensions: 177840
Number of successful extensions: 820
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 778
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 819
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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