BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0154
(629 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g10360.1 68417.m01701 expressed protein 29 2.5
At5g08450.2 68418.m00996 expressed protein KED, Nicotiana tabacu... 28 4.4
At5g08450.1 68418.m00995 expressed protein KED, Nicotiana tabacu... 28 4.4
At1g03350.1 68414.m00314 BSD domain-containing protein contains ... 28 4.4
At1g20130.1 68414.m02518 family II extracellular lipase, putativ... 28 5.9
At5g21430.1 68418.m02535 DNAJ heat shock N-terminal domain-conta... 27 7.8
At1g29940.1 68414.m03658 DNA-directed RNA polymerase family prot... 27 7.8
>At4g10360.1 68417.m01701 expressed protein
Length = 266
Score = 29.1 bits (62), Expect = 2.5
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = -1
Query: 488 LHILHFFIHSRTHFGHVRFLFPLFLYCRSSLAVALRTQFVL 366
L + FF+H HF V+ +FPL Y ++ AL +L
Sbjct: 202 LLFIFFFVHMYLHFHQVKQVFPLGFYSLLTIPPALAVMNLL 242
>At5g08450.2 68418.m00996 expressed protein KED, Nicotiana tabacum,
EMBL:AB009883
Length = 918
Score = 28.3 bits (60), Expect = 4.4
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 407 DNKETEEKENERDQSGFCYG*RNEEYANFGRRENKTGCV 523
D +ET+EK ERD G R E+ + +E++ GC+
Sbjct: 473 DERETKEKRRERD--GDSEAERAEKRSRISEKESEDGCL 509
>At5g08450.1 68418.m00995 expressed protein KED, Nicotiana tabacum,
EMBL:AB009883
Length = 918
Score = 28.3 bits (60), Expect = 4.4
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 407 DNKETEEKENERDQSGFCYG*RNEEYANFGRRENKTGCV 523
D +ET+EK ERD G R E+ + +E++ GC+
Sbjct: 473 DERETKEKRRERD--GDSEAERAEKRSRISEKESEDGCL 509
>At1g03350.1 68414.m00314 BSD domain-containing protein contains
Pfam profile PF03909: BSD domain
Length = 470
Score = 28.3 bits (60), Expect = 4.4
Identities = 15/43 (34%), Positives = 22/43 (51%)
Frame = +3
Query: 261 MNILKAFYVDLLVPLETNLEKDTKVVQSEQKRFLQQHKLRSES 389
MN K+ + + L P ET E D+ E + Q+H +SES
Sbjct: 1 MNFFKSVFTEDLDPPETESESDSPKHSEEHEHPEQEHPEQSES 43
>At1g20130.1 68414.m02518 family II extracellular lipase, putative
contains Pfam profile PF00657: GDSL-like
Lipase/Acylhydrolase; similar to EXL3 (PMID:11431566)
Length = 1006
Score = 27.9 bits (59), Expect = 5.9
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -3
Query: 192 RPMFLVAPACPASPLRPRRDETPPP 118
+P + PACP +P +P+ PPP
Sbjct: 59 QPKPVPPPACPPTPPKPQPKPAPPP 83
>At5g21430.1 68418.m02535 DNAJ heat shock N-terminal
domain-containing protein contains Pfam profile: PF00226
DnaJ domain;
Length = 218
Score = 27.5 bits (58), Expect = 7.8
Identities = 16/42 (38%), Positives = 23/42 (54%), Gaps = 1/42 (2%)
Frame = +3
Query: 468 KEMKNMQILEEE-KTKLDAFCEQSLKNAMTQERRRYGFVLER 590
KE+K + EE+ KTK+D E + +ERR Y + L R
Sbjct: 120 KELKEQGLDEEQLKTKMDLIKESYTILSTVEERRMYDWSLAR 161
>At1g29940.1 68414.m03658 DNA-directed RNA polymerase family protein
similar to SP|P22138 DNA-directed RNA polymerase I 135
kDa polypeptide (EC 2.7.7.6) (RNA polymerase I subunit
2) {Saccharomyces cerevisiae}; contains Pfam profiles
PF04563; RNA polymerase beta subunit, PF04560: RNA
polymerase Rpb2 domain 7, PF04561: RNA polymerase Rpb2
domain 2, PF04565: RNA polymerase Rpb2 domain 3,
PF00562: RNA polymerase Rpb2 domain 6
Length = 1114
Score = 27.5 bits (58), Expect = 7.8
Identities = 12/36 (33%), Positives = 24/36 (66%)
Frame = -3
Query: 180 LVAPACPASPLRPRRDETPPPHHVELLNMPFQQLSS 73
L +CPA +RP ++ + P ++EL+ PF+Q+++
Sbjct: 564 LYLASCPARFIRPVKNISIPSDNIELIG-PFEQVAN 598
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,237,277
Number of Sequences: 28952
Number of extensions: 260211
Number of successful extensions: 806
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 777
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 806
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1285411824
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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