Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0148
         (724 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces...   256   2e-69
SPBP23A10.07 |rpa2||DNA-directed RNA polymerase I complex subuni...    27   3.6  
SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces ...    26   4.7  
SPAC1751.03 ||SPAC31A2.01|translation initiation factor eIF3m|Sc...    26   4.7  
SPBC1105.10 |rav1||RAVE complex subunit Rav1 |Schizosaccharomyce...    26   6.3  
SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosa...    26   6.3  
SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosac...    25   8.3  

>SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1009

 Score =  256 bits (628), Expect = 2e-69
 Identities = 125/242 (51%), Positives = 166/242 (68%), Gaps = 2/242 (0%)
 Frame = +1

Query: 4   NIGIEFMFINSLEQCNWIRQRMEPPNVTKMNADQKRLILARLTRSTGFENFLAKKWSSEK 183
           +  +EF  I+S ++ NWI   +E P   + + DQK +I  RL+ +  FE FL  K+ ++K
Sbjct: 210 SFAVEFTHISSRKRSNWILSHLETPTPFRYSHDQKIMIFDRLSWADSFERFLFTKFPNDK 269

Query: 184 RFGLEGCEILIPAMKQVIDVSTKLGVESIIMGMPHRGRLNVLANVCRKPLHQLFTQFAGL 363
           RFGLEGCE ++P MK +ID S   G+ +I++GM HRGRLN+L N+ RKP   +F++F G 
Sbjct: 270 RFGLEGCEAMVPGMKALIDRSVDEGISNIVIGMAHRGRLNLLHNIVRKPAQAIFSEFRGT 329

Query: 364 -EAEDDGSGDVKYHLGTYIERLNRVTNKNIRLAVCANPSHLEAVDPVVQGKTRAEQFYRG 540
            + +D+GSGDVKYHLG   +R    + K + L++ ANPSHLEA DPVV GK RA Q Y  
Sbjct: 330 QDPDDEGSGDVKYHLGMNYQR-PTPSGKRVSLSLVANPSHLEAEDPVVLGKVRAIQHYTS 388

Query: 541 DNEG-KKVMSILLHGDAAFAGQGVVFETMHLSDLPAYTTHGTIHIVANNQIGFTTDPRHS 717
           D    ++ M IL+HGDAAFA QGVV+ET  L  LP Y+T GT+HIV NNQIGFTTDPR +
Sbjct: 389 DEASHEQSMGILIHGDAAFAAQGVVYETFGLHALPGYSTGGTVHIVINNQIGFTTDPRFA 448

Query: 718 RS 723
           RS
Sbjct: 449 RS 450


>SPBP23A10.07 |rpa2||DNA-directed RNA polymerase I complex subunit
           Rpa2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1227

 Score = 26.6 bits (56), Expect = 3.6
 Identities = 15/40 (37%), Positives = 21/40 (52%)
 Frame = +3

Query: 186 FRFGRVRNFDSGDEASHRRFHETRSRIHHYGHAP*RAPQR 305
           F +G V   +S D +  RR  E    +HH+G AP   P+R
Sbjct: 836 FGYGTVYKGESFDLSQKRRRGEPV--VHHFGFAPGSTPRR 873


>SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1098

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 8/25 (32%), Positives = 17/25 (68%)
 Frame = +2

Query: 299 STYSRTCAVSLCTSCSHSSPVSKLR 373
           S+++  C  ++ T+C++S+P   LR
Sbjct: 608 SSFAEACIQAIATNCNYSTPAELLR 632


>SPAC1751.03 ||SPAC31A2.01|translation initiation factor
           eIF3m|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 402

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 28/94 (29%), Positives = 43/94 (45%), Gaps = 9/94 (9%)
 Frame = -2

Query: 582 AVQQDRHHLLALVVSPVEL-----LGSGLA--LHHGVDGLQVRGVRANSQS--NVLVGNA 430
           AV++ +H  L+++ S V L     L S LA  L +  D L+  GV  +     N+ V  A
Sbjct: 124 AVKERQHARLSILTSVVTLTTRYSLFSTLAPNLKYFPDWLKEAGVSVSDHRAFNIFVSKA 183

Query: 429 IQTLDVRAQVIFHVAGTIILSFETGELCEQLVQR 328
           IQ+ D      F +    + +    E   +LVQR
Sbjct: 184 IQSYDDEQSFAFLLEAVKMDNSTADEAVRELVQR 217


>SPBC1105.10 |rav1||RAVE complex subunit Rav1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1297

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 15/51 (29%), Positives = 25/51 (49%)
 Frame = -3

Query: 350 CVNSWCRGLRHTFASTLRRPLWGMPIMMDSTPSFVETSMTCFIAGIKISHP 198
           C +S+ + +   F+    R +W    + DS  S   +S  CFIA I+  +P
Sbjct: 115 CASSYTKLIN--FSEDKPRLVWSQNRISDSFLSSTISSDACFIASIETKNP 163


>SPCC550.03c |||RNA helicase involved in mRNA
           catabolism|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1213

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 13/33 (39%), Positives = 18/33 (54%)
 Frame = -2

Query: 594 ERRVAVQQDRHHLLALVVSPVELLGSGLALHHG 496
           E+ VA  +    LL  +    E+L  GLA+HHG
Sbjct: 589 EKAVARLKKEDRLLPQIGRMREMLSRGLAVHHG 621


>SPCC777.14 |prp4||serine/threonine protein kinase
           Prp4|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 477

 Score = 25.4 bits (53), Expect = 8.3
 Identities = 14/34 (41%), Positives = 21/34 (61%)
 Frame = +1

Query: 19  FMFINSLEQCNWIRQRMEPPNVTKMNADQKRLIL 120
           FM ++ L+QCN I   ++P N   M  ++KR IL
Sbjct: 271 FMALDLLKQCNVIHSDIKPDN---MLVNEKRNIL 301


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,026,241
Number of Sequences: 5004
Number of extensions: 61780
Number of successful extensions: 176
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 339215786
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)