Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0148
(724 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 compone... 295 2e-80
At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 compone... 293 1e-79
At5g44635.1 68418.m05469 minichromosome maintenance family prote... 29 4.1
At3g23530.1 68416.m02961 cyclopropane fatty acid synthase, putat... 29 4.1
At3g23510.1 68416.m02960 cyclopropane fatty acid synthase, putat... 29 4.1
At1g53840.1 68414.m06128 pectinesterase family protein contains ... 28 5.5
At4g22030.1 68417.m03187 F-box family protein PF0064: F-box doma... 28 7.2
At1g15310.1 68414.m01832 signal recognition particle 54 kDa prot... 28 7.2
At3g50210.2 68416.m05490 2-oxoacid-dependent oxidase, putative s... 27 9.5
At3g50210.1 68416.m05491 2-oxoacid-dependent oxidase, putative s... 27 9.5
At3g06770.3 68416.m00803 glycoside hydrolase family 28 protein /... 27 9.5
At3g06770.2 68416.m00804 glycoside hydrolase family 28 protein /... 27 9.5
At3g06770.1 68416.m00802 glycoside hydrolase family 28 protein /... 27 9.5
>At5g65750.1 68418.m08274 2-oxoglutarate dehydrogenase E1 component,
putative / oxoglutarate decarboxylase, putative /
alpha-ketoglutaric dehydrogenase, putative similar to
SP|P20967 2-oxoglutarate dehydrogenase E1 component,
mitochondrial precursor (EC 1.2.4.2)
(Alpha-ketoglutarate dehydrogenase) {Saccharomyces
cerevisiae}; contains Pfam profiles PF02779:
Transketolase, pyridine binding domain, PF00676:
Dehydrogenase E1 component
Length = 1025
Score = 295 bits (724), Expect = 2e-80
Identities = 142/245 (57%), Positives = 179/245 (73%), Gaps = 6/245 (2%)
Frame = +1
Query: 7 IGIEFMFINSLEQCNWIRQRMEPPNVTKMNADQKRLILARLTRSTGFENFLAKKWSSEKR 186
IG E+M I ++CNW+R ++E P + N++++ +I RLT ST FENFLA KW++ KR
Sbjct: 206 IGYEYMHIADRDKCNWLRDKIETPTPRQYNSERRMVIYDRLTWSTQFENFLATKWTTAKR 265
Query: 187 FGLEGCEILIPAMKQVIDVSTKLGVESIIMGMPHRGRLNVLANVCRKPLHQLFTQFAGLE 366
FGLEG E LIP MK++ D S LGVE+I++GMPHRGRLNVL NV RKPL Q+F++F+G
Sbjct: 266 FGLEGAESLIPGMKEMFDRSADLGVENIVIGMPHRGRLNVLGNVVRKPLRQIFSEFSGGT 325
Query: 367 AEDD------GSGDVKYHLGTYIERLNRVTNKNIRLAVCANPSHLEAVDPVVQGKTRAEQ 528
D G+GDVKYHLGT +R R K++ L++ ANPSHLEAVDPVV GKTRA+Q
Sbjct: 326 RPVDEVGLYTGTGDVKYHLGTSYDRPTR-GGKHLHLSLVANPSHLEAVDPVVIGKTRAKQ 384
Query: 529 FYRGDNEGKKVMSILLHGDAAFAGQGVVFETMHLSDLPAYTTHGTIHIVANNQIGFTTDP 708
+Y D K M IL+HGD +FAGQGVV+ET+HLS LP Y T GT+HIV NNQ+ FTTDP
Sbjct: 385 YYTKDENRTKNMGILIHGDGSFAGQGVVYETLHLSALPNYCTGGTVHIVVNNQVAFTTDP 444
Query: 709 RHSRS 723
R RS
Sbjct: 445 REGRS 449
>At3g55410.1 68416.m06154 2-oxoglutarate dehydrogenase E1 component,
putative / oxoglutarate decarboxylase, putative /
alpha-ketoglutaric dehydrogenase, putative similar to
SP|P20967 2-oxoglutarate dehydrogenase E1 component,
mitochondrial precursor (EC 1.2.4.2)
(Alpha-ketoglutarate dehydrogenase) {Saccharomyces
cerevisiae}; contains Pfam profiles PF02779:
Transketolase, pyridine binding domain, PF00676:
Dehydrogenase E1 component
Length = 1017
Score = 293 bits (718), Expect = 1e-79
Identities = 142/245 (57%), Positives = 180/245 (73%), Gaps = 5/245 (2%)
Frame = +1
Query: 4 NIGIEFMFINSLEQCNWIRQRMEPPNVTKMNADQKRLILARLTRSTGFENFLAKKWSSEK 183
NIG E+M I ++CNW+R+++E P + N +++ +IL RL ST FENFLA KW++ K
Sbjct: 202 NIGFEYMHIADRDKCNWLREKIETPTPWRYNRERREVILDRLAWSTQFENFLATKWTTAK 261
Query: 184 RFGLEGCEILIPAMKQVIDVSTKLGVESIIMGMPHRGRLNVLANVCRKPLHQLFTQFA-G 360
RFGLEG E LIP MK++ D + LGVESI++GM HRGRLNVL NV RKPL Q+F++F+ G
Sbjct: 262 RFGLEGGESLIPGMKEMFDRAADLGVESIVIGMSHRGRLNVLGNVVRKPLRQIFSEFSGG 321
Query: 361 LEAEDD----GSGDVKYHLGTYIERLNRVTNKNIRLAVCANPSHLEAVDPVVQGKTRAEQ 528
+ D+ G+GDVKYHLGT +R R K I L++ ANPSHLEA D VV GKTRA+Q
Sbjct: 322 IRPVDEVGYTGTGDVKYHLGTSYDRPTR-GGKKIHLSLVANPSHLEAADSVVVGKTRAKQ 380
Query: 529 FYRGDNEGKKVMSILLHGDAAFAGQGVVFETMHLSDLPAYTTHGTIHIVANNQIGFTTDP 708
+Y D + K + IL+HGD +FAGQGVV+ET+HLS LP YTT GTIHIV NNQ+ FTTDP
Sbjct: 381 YYSNDLDRTKNLGILIHGDGSFAGQGVVYETLHLSALPNYTTGGTIHIVVNNQVAFTTDP 440
Query: 709 RHSRS 723
R RS
Sbjct: 441 RAGRS 445
>At5g44635.1 68418.m05469 minichromosome maintenance family protein
/ MCM family protein similar to SP|P97311 DNA
replication licensing factor MCM6 {Mus musculus};
contains Pfam profile PF00493: MCM2/3/5 family
Length = 831
Score = 28.7 bits (61), Expect = 4.1
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = -2
Query: 519 SGLALHHGVDGLQVRGVRANSQSNVLVGNAIQTLD 415
S A H GV GL+ GVR S + N++Q D
Sbjct: 272 SSTAGHEGVQGLKALGVRDLSYRLAFIANSVQIAD 306
>At3g23530.1 68416.m02961 cyclopropane fatty acid synthase, putative
/ CPA-FA synthase, putative similar to cyclopropane
synthase [Sterculia foetida] GI:21069167; contains Pfam
profiles PF02353: Cyclopropane-fatty-acyl-phospholipid
synthase, PF01593: amine oxidase, flavin-containing
Length = 867
Score = 28.7 bits (61), Expect = 4.1
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +2
Query: 269 SLWACPIEGASTYSRTCAVSLCTSCSH 349
S+W+CP +G ++S +S C C+H
Sbjct: 173 SIWSCPSDGVLSFSAYSVLSFC--CNH 197
>At3g23510.1 68416.m02960 cyclopropane fatty acid synthase, putative
/ CPA-FA synthase, putative similar to cyclopropane
synthase [Sterculia foetida] GI:21069167; contains Pfam
profile PF02353: Cyclopropane-fatty-acyl-phospholipid
synthase
Length = 867
Score = 28.7 bits (61), Expect = 4.1
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +2
Query: 269 SLWACPIEGASTYSRTCAVSLCTSCSH 349
S+W+CP +G ++S +S C C+H
Sbjct: 173 SIWSCPSDGVLSFSAYSVLSFC--CNH 197
>At1g53840.1 68414.m06128 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 586
Score = 28.3 bits (60), Expect = 5.5
Identities = 21/73 (28%), Positives = 37/73 (50%)
Frame = +1
Query: 349 QFAGLEAEDDGSGDVKYHLGTYIERLNRVTNKNIRLAVCANPSHLEAVDPVVQGKTRAEQ 528
Q AGL+ + +GD + T E + +V K++++ V S V+ VV K++
Sbjct: 273 QTAGLKPDVTVAGDGTGDVLTVNEAVAKVPKKSLKMFVIYVKSGTY-VENVVMDKSKWNV 331
Query: 529 FYRGDNEGKKVMS 567
GD +GK ++S
Sbjct: 332 MIYGDGKGKTIIS 344
>At4g22030.1 68417.m03187 F-box family protein PF0064: F-box domain;
similar to F-box protein family, AtFBX7 (GI:20197899)
[Arabidopsis thaliana]
Length = 626
Score = 27.9 bits (59), Expect = 7.2
Identities = 11/37 (29%), Positives = 20/37 (54%)
Frame = +2
Query: 431 ALPTRTLDWLFARTPRTWRPSTPWCRARPEPSSSTGE 541
A P + + + P+ ++P+T W +PEP+ S E
Sbjct: 221 AYPLPLVGTMLEKFPQEFKPATWWPETKPEPTHSRTE 257
>At1g15310.1 68414.m01832 signal recognition particle 54 kDa protein
1 / SRP54 (SRP-54) (SRP-54A) identical to
Swiss-Prot:P37106 signal recognition particle 54 kDa
protein 1 (SRP54) [Arabidopsis thaliana]
Length = 479
Score = 27.9 bits (59), Expect = 7.2
Identities = 29/108 (26%), Positives = 52/108 (48%), Gaps = 6/108 (5%)
Frame = +1
Query: 145 FENFLAKKWSSEKRFGLEGCEILIPAMKQVI--DVSTKLGVESIIMG-MPHRGRLNVLAN 315
FE F K + S + G L+ +++V+ D+ +L VE++ G R +
Sbjct: 282 FEVFDVKPFVS-RLLGKGDWSGLVDKLQEVVPKDLQNEL-VENLSQGNFTLRSMYDQFQC 339
Query: 316 VCRKPLHQLFTQFAGLEAE--DDGSG-DVKYHLGTYIERLNRVTNKNI 450
R PL+QLF+ G+ AE G G + + + Y+ ++ +TNK +
Sbjct: 340 SLRIPLNQLFSMLPGISAEMMPKGHGEESRVKMKRYMTMMDSMTNKEL 387
>At3g50210.2 68416.m05490 2-oxoacid-dependent oxidase, putative
strong similarity to partial cds of 2-oxoacid-dependent
oxidase (din11) from GI:10834554 [Arabidopsis thaliana]
Length = 250
Score = 27.5 bits (58), Expect = 9.5
Identities = 17/60 (28%), Positives = 27/60 (45%)
Frame = +1
Query: 406 GTYIERLNRVTNKNIRLAVCANPSHLEAVDPVVQGKTRAEQFYRGDNEGKKVMSILLHGD 585
G Y L+RV N + + VC + D VV+ +Q Y G G +V ++G+
Sbjct: 179 GVYESTLHRVINNSPQYRVCVAFFYETNFDAVVEPLDICKQKYPGGRGGCQVFKRAVYGE 238
>At3g50210.1 68416.m05491 2-oxoacid-dependent oxidase, putative
strong similarity to partial cds of 2-oxoacid-dependent
oxidase (din11) from GI:10834554 [Arabidopsis thaliana]
Length = 332
Score = 27.5 bits (58), Expect = 9.5
Identities = 17/60 (28%), Positives = 27/60 (45%)
Frame = +1
Query: 406 GTYIERLNRVTNKNIRLAVCANPSHLEAVDPVVQGKTRAEQFYRGDNEGKKVMSILLHGD 585
G Y L+RV N + + VC + D VV+ +Q Y G G +V ++G+
Sbjct: 261 GVYESTLHRVINNSPQYRVCVAFFYETNFDAVVEPLDICKQKYPGGRGGCQVFKRAVYGE 320
>At3g06770.3 68416.m00803 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein weak
similarity to polygalacturonase [Persea americana]
GI:166951; contains PF00295: Glycosyl hydrolases family
28 (polygalacturonases)
Length = 377
Score = 27.5 bits (58), Expect = 9.5
Identities = 20/78 (25%), Positives = 35/78 (44%), Gaps = 4/78 (5%)
Frame = +1
Query: 469 NPSHLEAVDPVV---QGKTRAEQFYRGDNEGKKVMSILLHGD-AAFAGQGVVFETMHLSD 636
+PSH E VDP+ +G + Y+ G K+ +++ GD GQG+V+ S
Sbjct: 44 DPSHWEVVDPLPSYGRGIDLPGKRYKSLINGNKLHDVVVTGDNGTIDGQGLVWWDRFTSH 103
Query: 637 LPAYTTHGTIHIVANNQI 690
Y I +++ +
Sbjct: 104 SLKYNRPHLIEFLSSENV 121
>At3g06770.2 68416.m00804 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein weak
similarity to polygalacturonase [Persea americana]
GI:166951; contains PF00295: Glycosyl hydrolases family
28 (polygalacturonases)
Length = 446
Score = 27.5 bits (58), Expect = 9.5
Identities = 20/78 (25%), Positives = 35/78 (44%), Gaps = 4/78 (5%)
Frame = +1
Query: 469 NPSHLEAVDPVV---QGKTRAEQFYRGDNEGKKVMSILLHGD-AAFAGQGVVFETMHLSD 636
+PSH E VDP+ +G + Y+ G K+ +++ GD GQG+V+ S
Sbjct: 113 DPSHWEVVDPLPSYGRGIDLPGKRYKSLINGNKLHDVVVTGDNGTIDGQGLVWWDRFTSH 172
Query: 637 LPAYTTHGTIHIVANNQI 690
Y I +++ +
Sbjct: 173 SLKYNRPHLIEFLSSENV 190
>At3g06770.1 68416.m00802 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein weak
similarity to polygalacturonase [Persea americana]
GI:166951; contains PF00295: Glycosyl hydrolases family
28 (polygalacturonases)
Length = 377
Score = 27.5 bits (58), Expect = 9.5
Identities = 20/78 (25%), Positives = 35/78 (44%), Gaps = 4/78 (5%)
Frame = +1
Query: 469 NPSHLEAVDPVV---QGKTRAEQFYRGDNEGKKVMSILLHGD-AAFAGQGVVFETMHLSD 636
+PSH E VDP+ +G + Y+ G K+ +++ GD GQG+V+ S
Sbjct: 44 DPSHWEVVDPLPSYGRGIDLPGKRYKSLINGNKLHDVVVTGDNGTIDGQGLVWWDRFTSH 103
Query: 637 LPAYTTHGTIHIVANNQI 690
Y I +++ +
Sbjct: 104 SLKYNRPHLIEFLSSENV 121
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,401,832
Number of Sequences: 28952
Number of extensions: 343974
Number of successful extensions: 995
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 953
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 989
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1575119672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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