Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0146
(701 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 111 7e-26
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 100 4e-22
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 97 2e-21
SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc... 92 8e-20
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 85 9e-18
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 83 4e-17
SPBC215.11c |||aldo/keto reductase, unknown biological role|Schi... 55 9e-09
SPCC737.06c |||glutamate-cysteine ligase regulatory subunit |Sch... 40 3e-04
SPCC1281.04 |||pyridoxal reductase |Schizosaccharomyces pombe|ch... 38 0.001
SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho... 30 0.28
SPAC9E9.11 |plr1|plr|pyridoxal reductase |Schizosaccharomyces po... 30 0.37
SPAC31G5.10 |eta2||Myb family transcriptional regulator Eta2|Sch... 29 0.64
SPAC823.14 |||phosphoric monoester hydrolase |Schizosaccharomyce... 29 0.85
SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces pombe... 28 1.5
SPAC17D4.02 |cdc45|sna41, goa1|DNA replication pre-initiation co... 27 2.6
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 27 3.4
SPBC582.08 |||alanine aminotransferase |Schizosaccharomyces pomb... 26 6.0
SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch... 26 6.0
SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase YakC|Schizo... 26 6.0
SPAPB1E7.05 |gde1||glycerophosphoryl diester phosphodiesterase G... 25 7.9
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 111 bits (268), Expect = 7e-26
Identities = 57/155 (36%), Positives = 95/155 (61%), Gaps = 1/155 (0%)
Frame = +2
Query: 11 DYLDTWKGMEEAKNLGLAKSIGISNFNTTQIDRILENG-QIKPSVLQVEVNLHLGQDKLI 187
D +++WK +E+ G ++IG+SNF I +L++ +I P V Q+E++ Q K++
Sbjct: 116 DRIESWKALEKGVEEGKLRAIGVSNFGPHHIQELLDSHPKIIPCVNQIELHPFCSQQKVV 175
Query: 188 DYCKRNGIVVMAFSPFGPMFHDSLPSIRPDDPTLVAIAEKHGKSVTQIVLRYLIQKGVVP 367
DYC+ GI + A++P + H + + L+AIA K+ KS QI++RY +Q+G +
Sbjct: 176 DYCESKGIQLAAYAP---LVHGE----KFGNKQLLAIASKYNKSEAQIMIRYCLQRGFIV 228
Query: 368 IPKSVKKNRIEENIDVFDFNLSSEDMNKIGTFNKE 472
+PKS RI+EN DVFDF +S EDM K+ +++
Sbjct: 229 LPKSSTPRRIKENGDVFDFEISKEDMEKLYNLDED 263
>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 99.5 bits (237), Expect = 4e-22
Identities = 51/150 (34%), Positives = 90/150 (60%), Gaps = 1/150 (0%)
Frame = +2
Query: 23 TWKGMEEAKNLGLAKSIGISNFNTTQIDRIL-ENGQIKPSVLQVEVNLHLGQDKLIDYCK 199
+WK MEE + G +S+G+SN+ + + N + P V Q+E++ L QD ++ YC+
Sbjct: 127 SWKAMEEFVDSGDIRSVGVSNYGVKHLQELYASNPKFYPCVNQIELHPFLSQDDIVKYCQ 186
Query: 200 RNGIVVMAFSPFGPMFHDSLPSIRPDDPTLVAIAEKHGKSVTQIVLRYLIQKGVVPIPKS 379
+ I + A+SP + H IR +D LV IA+K SV Q+++R+ +QKG +PI KS
Sbjct: 187 SHDIAIEAYSP---LTH----GIRLNDEKLVPIAKKLNISVAQLLIRWSLQKGYIPIIKS 239
Query: 380 VKKNRIEENIDVFDFNLSSEDMNKIGTFNK 469
KK + ++DVF+F + + + ++ +F++
Sbjct: 240 TKKEHMLSDLDVFNFTIPDDVVQELSSFDE 269
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 97.1 bits (231), Expect = 2e-21
Identities = 56/158 (35%), Positives = 91/158 (57%), Gaps = 5/158 (3%)
Frame = +2
Query: 20 DTWKGMEEAKNLGLAKSIGISNFNTTQIDRILENGQIKPSVLQVEVNLHLGQDKLIDYCK 199
+TWK ME+ G + IG+SNFN T ++RIL+ ++KP+V Q+E++ L Q + ++ K
Sbjct: 147 ETWKAMEKLLETGKVRHIGLSNFNDTNLERILKVAKVKPAVHQMELHPFLPQTEFVEKHK 206
Query: 200 RNGIVVMAFSPFG---PMFHDSLPSIRPDDPTLVAIAEKHGKSVT--QIVLRYLIQKGVV 364
+ GI V A+SPFG ++ +P + + T+ IA+ G+ VT I + + I +G
Sbjct: 207 KLGIHVTAYSPFGNQNTIYESKIPKL-IEHETIQKIAKSKGEGVTGATIAVSWAITRGTS 265
Query: 365 PIPKSVKKNRIEENIDVFDFNLSSEDMNKIGTFNKEKR 478
IPKSV + RI+ N L+ EDM++I + R
Sbjct: 266 VIPKSVNEQRIKSNFKYIP--LTKEDMDEINSIGIRAR 301
>SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde
reductase AKR3C2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 284
Score = 91.9 bits (218), Expect = 8e-20
Identities = 49/156 (31%), Positives = 84/156 (53%), Gaps = 4/156 (2%)
Frame = +2
Query: 26 WKGMEEAKNLGLAKSIGISNFNTTQIDRILENGQIKPSVLQVEVN--LHLGQDKLIDYCK 199
WK ME A GL S+G+SNF ++ +L+ I P V Q+E + ++ L+++C+
Sbjct: 125 WKAMETALGTGLVHSVGVSNFRIPDLEELLKTSTITPRVNQIEFHPQVYKAAKPLVEFCQ 184
Query: 200 RNGIVVMAFSPFGPMFHDSLPSIRPDDPTLVAIAEKHGKSVTQIVLRYLIQKGVVPIPKS 379
GI+V + P P+ D+ + +L K+ S TQI+L++ KGV+PI +
Sbjct: 185 SKGIIVEGYGPLSPLVRDAQGPVAEFTKSL---ESKYHVSDTQILLKWAYSKGVIPITTT 241
Query: 380 VKKNRIEENIDVFDFNLSSEDMNKIGTFNKE--KRT 481
K R++E ++ F L D++++GT + KRT
Sbjct: 242 SKIERMKECLNFDSFTLDKADIDELGTLGVQHHKRT 277
>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 276
Score = 85.0 bits (201), Expect = 9e-18
Identities = 47/152 (30%), Positives = 80/152 (52%)
Frame = +2
Query: 17 LDTWKGMEEAKNLGLAKSIGISNFNTTQIDRILENGQIKPSVLQVEVNLHLGQDKLIDYC 196
+++W+ + + + G IG+SN+N ++ I+ G P++ QVE + + + YC
Sbjct: 119 IESWRALLQHQKEGRINKIGVSNYNIHHLEEIISLGLPLPAINQVEFSAFNNRPTFLSYC 178
Query: 197 KRNGIVVMAFSPFGPMFHDSLPSIRPDDPTLVAIAEKHGKSVTQIVLRYLIQKGVVPIPK 376
+GI+V AFSP R D L+ ++ K+ K+ I+LRY +QKGV PI K
Sbjct: 179 FNHGILVQAFSPL-------TRGYRLSDIRLLDLSLKYNKTPANILLRYCLQKGVSPIFK 231
Query: 377 SVKKNRIEENIDVFDFNLSSEDMNKIGTFNKE 472
+ I EN+ F L DM+ + T+++E
Sbjct: 232 ASSFVHIHENVKAEQFMLDPSDMDVMDTWDEE 263
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 83.0 bits (196), Expect = 4e-17
Identities = 50/155 (32%), Positives = 85/155 (54%), Gaps = 5/155 (3%)
Frame = +2
Query: 20 DTWKGMEEAKNLGLAKSIGISNFNTTQIDRILENGQIKPSVLQVEVNLHLGQDKLIDYCK 199
+TW+ MEE G + IGISNFN +DR+L+ ++KP++ Q+E++ +L Q + ++ K
Sbjct: 146 ETWQAMEELLETGKVRYIGISNFNNEYLDRVLKIAKVKPTIHQMELHPYLPQTEYLEKHK 205
Query: 200 RNGIVVMAFSPFGPM--FHDSLPSIRPDDPTLVAIAEKHGKSVT--QIVLRYLIQKGVVP 367
+ I V A+SP ++S S + TLV IA G+ +T I + + +++G
Sbjct: 206 KLQIHVSAYSPLANQNDAYNSDISKLIEHKTLVDIANARGEGITPANIAISWAVKRGTSV 265
Query: 368 IPKSVKKNRIEENIDVFDF-NLSSEDMNKIGTFNK 469
+PKSV ++RI N + E +N IG +
Sbjct: 266 LPKSVNESRIVSNFLYIPLTDKEMEAINNIGVVRR 300
>SPBC215.11c |||aldo/keto reductase, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 306
Score = 55.2 bits (127), Expect = 9e-09
Identities = 47/156 (30%), Positives = 80/156 (51%), Gaps = 4/156 (2%)
Frame = +2
Query: 20 DTWKGMEEAKNLGLAKSIGISNFNTTQIDRILENGQIKPSV-LQVEVNL-HLGQDKLIDY 193
D + + K GL + +G+S +D I E Q P V +Q NL + +K+++Y
Sbjct: 147 DQFSEIAAMKKEGLIRHVGLSEVT---VDDIKEAEQYFPVVSVQNLFNLVNRKNEKVLEY 203
Query: 194 CKRNGIVVMAFSPFGPMFHDSLPSIRPDDPTLVAIAEKHGKSVTQIVLRYLIQKGVV--P 367
C++ GI AF P+ P+ +L +P L A+++ +S +QI L +++Q+ V P
Sbjct: 204 CEQKGI---AFIPWYPLASGALA--KPGT-ILDAVSKDLDRSTSQIALSWVLQRSPVMLP 257
Query: 368 IPKSVKKNRIEENIDVFDFNLSSEDMNKIGTFNKEK 475
IP + K + +EEN+ LSSE K+ K +
Sbjct: 258 IPGTSKVDHLEENVKAAGIQLSSEVFAKLDEEGKSE 293
>SPCC737.06c |||glutamate-cysteine ligase regulatory subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 287
Score = 40.3 bits (90), Expect = 3e-04
Identities = 21/87 (24%), Positives = 44/87 (50%), Gaps = 2/87 (2%)
Frame = +2
Query: 2 DIVDYLDTWKGMEEAKNLGLAKSIGISNFNTTQIDRILENGQIKPSVLQVEV--NLHLGQ 175
+I ++DTWK +EE G ++G+S F ++ R++ + + P Q+ + N L
Sbjct: 154 EIQSWVDTWKLLEEKVGEGKIGTLGVSEFGVNELQRLISSVNVVPESTQINIGQNCKLPN 213
Query: 176 DKLIDYCKRNGIVVMAFSPFGPMFHDS 256
D L+++ R+ + + S + +S
Sbjct: 214 D-LLNFADRHHLKLFFHSDPSALLSES 239
>SPCC1281.04 |||pyridoxal reductase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 333
Score = 37.9 bits (84), Expect = 0.001
Identities = 22/77 (28%), Positives = 40/77 (51%), Gaps = 4/77 (5%)
Frame = +2
Query: 233 FGP-MFHDSLPSIRPDDPTLVAIAEKHGKSVTQIVLRYLIQKG---VVPIPKSVKKNRIE 400
F P +F ++P ++ + +A+K G S+ + L ++I G ++PIP S R E
Sbjct: 236 FNPKVFEKNIPFLK----AVEQLAQKFGMSMPEFALNFIIANGKGMIIPIPGSTTVQRAE 291
Query: 401 ENIDVFDFNLSSEDMNK 451
N+ +LSSE + +
Sbjct: 292 SNLSALKKSLSSEQLEE 308
>SPBC3E7.01 |fab1|ste12,
SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase
Fab1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 1932
Score = 30.3 bits (65), Expect = 0.28
Identities = 16/47 (34%), Positives = 27/47 (57%)
Frame = +2
Query: 356 GVVPIPKSVKKNRIEENIDVFDFNLSSEDMNKIGTFNKEKRTVGMSF 496
G VP ++KN+ + V D LS+++ N++ F+K R G+SF
Sbjct: 253 GKVPSYSKLRKNKAFSSAKVSDMYLSADERNRLEDFSKGDR--GLSF 297
>SPAC9E9.11 |plr1|plr|pyridoxal reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 333
Score = 29.9 bits (64), Expect = 0.37
Identities = 41/177 (23%), Positives = 80/177 (45%), Gaps = 34/177 (19%)
Frame = +2
Query: 23 TWKGMEEAKNLGLAKSIGISNFNTTQIDRILENGQIKPSVLQVEVNLH---LGQDKLIDY 193
T K ++ + G +G+S + I R + + + ++VE +L + + ++D
Sbjct: 134 TMKTLKGFVDSGKISCVGLSEVSAETIKRA--HAVVPIAAVEVEYSLFSRDIETNGIMDI 191
Query: 194 CKRNGIVVMAFSPF--GPM------------FHDSLPSIR------PDD-----PTLVAI 298
C++ I ++A+SPF G + F S P + PD P L A+
Sbjct: 192 CRKLSIPIIAYSPFCRGLLTGRIKTVEDLKEFAKSFPFLEYLDRFSPDVFAKNLPFLQAV 251
Query: 299 ---AEKHGKSVTQIVLRYLIQKG---VVPIPKSVKKNRIEENIDVFDFNLSSEDMNK 451
A+K G ++ + L +++ G V+PIP S +R + N++ + +LS E +
Sbjct: 252 EQLAKKFGMTMPEFSLLFIMASGNGLVIPIPGSTSVSRTKSNLNALNKSLSPEQFKE 308
>SPAC31G5.10 |eta2||Myb family transcriptional regulator
Eta2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 569
Score = 29.1 bits (62), Expect = 0.64
Identities = 16/52 (30%), Positives = 26/52 (50%)
Frame = +2
Query: 2 DIVDYLDTWKGMEEAKNLGLAKSIGISNFNTTQIDRILENGQIKPSVLQVEV 157
+ +D W EEAK L L KS S+F+T ++ + + S +Q E+
Sbjct: 377 ETIDRRKLWTNEEEAKLLDLVKSSYRSSFHTKKMTSLFTHNNHTTSNIQREI 428
>SPAC823.14 |||phosphoric monoester hydrolase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 229
Score = 28.7 bits (61), Expect = 0.85
Identities = 14/61 (22%), Positives = 33/61 (54%), Gaps = 2/61 (3%)
Frame = +2
Query: 59 LAKSIGISNFNTTQIDRILENGQI--KPSVLQVEVNLHLGQDKLIDYCKRNGIVVMAFSP 232
L + G+ N N +++ + +G I + + ++ ++HL D+ ++ K+N + +F P
Sbjct: 26 LTDNFGMGNANRVNLNQQVLDGSISFRDAFAKMLDSVHLSYDEALEVLKKNVAIDPSFKP 85
Query: 233 F 235
F
Sbjct: 86 F 86
>SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 526
Score = 27.9 bits (59), Expect = 1.5
Identities = 19/63 (30%), Positives = 35/63 (55%), Gaps = 1/63 (1%)
Frame = +2
Query: 284 TLVAIAEKHGKSVTQIVLRYLIQKGVVPIPKSVKKNRIEENIDVFD-FNLSSEDMNKIGT 460
T +A+ K + V++I L+Y QK + +P+ + ++ VFD L+ MN+I T
Sbjct: 97 TQLALKPKTTQQVSEI-LKYCNQKKLAVVPQGGNTGLVGGSVPVFDEIVLNLGLMNQIHT 155
Query: 461 FNK 469
F++
Sbjct: 156 FDE 158
>SPAC17D4.02 |cdc45|sna41, goa1|DNA replication pre-initiation
complex subunit Cdc45|Schizosaccharomyces pombe|chr
1|||Manual
Length = 638
Score = 27.1 bits (57), Expect = 2.6
Identities = 14/28 (50%), Positives = 16/28 (57%)
Frame = +2
Query: 371 PKSVKKNRIEENIDVFDFNLSSEDMNKI 454
P ++ IE NI VFD EDMNKI
Sbjct: 113 PHNLNNIYIENNIFVFDDGDIEEDMNKI 140
>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1016
Score = 26.6 bits (56), Expect = 3.4
Identities = 14/47 (29%), Positives = 25/47 (53%)
Frame = -3
Query: 159 FTSTCKTEGFIWPFSNILSICVVLKLDMPIDLARPRFLASSMPFQVS 19
F++T + F SNI S +L D P+ +++ FL + F++S
Sbjct: 87 FSTTKSAKSFSSTSSNIDSNLDLLNYDTPLTISKISFLLQQLEFKLS 133
>SPBC582.08 |||alanine aminotransferase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 505
Score = 25.8 bits (54), Expect = 6.0
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = +2
Query: 266 IRPDDPTLVAIAEKHGKSVTQIVLRYLIQKGVVPIPKSVKKNRIEEN 406
I P +PT I+E + V LR+ KG+V + V +N I +N
Sbjct: 250 INPGNPTGACISENSMEKV----LRFAKAKGIVLLADEVYQNNIYQN 292
>SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1318
Score = 25.8 bits (54), Expect = 6.0
Identities = 15/72 (20%), Positives = 34/72 (47%), Gaps = 1/72 (1%)
Frame = -3
Query: 495 KDIPTVRFSLL-NVPILFISSDDKLKSKTSMFSSMRFFLTDLGIGTTPFWIKYRRTICVT 319
+++P +L+ N + + + +K+ + SS+ F + D+ I ++ + ++C
Sbjct: 865 RELPLETITLIFNALVDSLPTSKHIKNSNDILSSV-FTINDMLIDEMTAYLSKKESLCSG 923
Query: 318 LFPCFSAMATSV 283
F C A SV
Sbjct: 924 TFSCLLACCNSV 935
>SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase
YakC|Schizosaccharomyces pombe|chr 1|||Manual
Length = 340
Score = 25.8 bits (54), Expect = 6.0
Identities = 15/55 (27%), Positives = 28/55 (50%), Gaps = 2/55 (3%)
Frame = +2
Query: 296 IAEKHGKSVTQIVLRYLIQKG--VVPIPKSVKKNRIEENIDVFDFNLSSEDMNKI 454
IA + + Q+ L +L+ +G ++PIP + + +EEN LS + +I
Sbjct: 255 IATANNITPGQLSLAWLLAQGDDILPIPGTKRVKYLEENFGALKVKLSDATVKEI 309
>SPAPB1E7.05 |gde1||glycerophosphoryl diester phosphodiesterase
Gde1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1076
Score = 25.4 bits (53), Expect = 7.9
Identities = 16/58 (27%), Positives = 27/58 (46%)
Frame = +2
Query: 11 DYLDTWKGMEEAKNLGLAKSIGISNFNTTQIDRILENGQIKPSVLQVEVNLHLGQDKL 184
D+L++W + A GL++ + I + +D + +G V LHL DKL
Sbjct: 397 DFLNSWTPLMLASATGLSEIVSILVASGASVDEVDSSGWTAMEQAVVRGYLHLA-DKL 453
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,902,337
Number of Sequences: 5004
Number of extensions: 58867
Number of successful extensions: 184
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 325165428
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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