Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0121
(620 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 226 2e-60
SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 196 2e-51
SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 98 8e-22
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.10
SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 31 0.13
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 0.95
SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces pombe... 28 1.3
SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr ... 27 1.7
SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 2.2
SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyce... 26 3.8
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 6.7
SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large... 25 8.8
SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |S... 25 8.8
SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ... 25 8.8
>SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 161
Score = 226 bits (552), Expect = 2e-60
Identities = 111/147 (75%), Positives = 130/147 (88%)
Frame = +3
Query: 78 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 257
P+Y PFFGVMG +AI+F++ GAAYGTAK+G GI+AM V+RP+LI+K+ IPVVMAGIIAI
Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISAMGVLRPDLIVKNTIPVVMAGIIAI 66
Query: 258 YGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQP 437
YGLVV+VLI+G L++ + LY GFI LGAGL+VG +GLAAGFAIGIVGDAGVRGTAQQP
Sbjct: 67 YGLVVSVLISGNLKQILS--LYSGFIQLGAGLSVGLAGLAAGFAIGIVGDAGVRGTAQQP 124
Query: 438 RLFVGMILILIFAEVLGLYGLIVAIYL 518
RLFV MILILIFAEVLGLYGLIVA+ L
Sbjct: 125 RLFVAMILILIFAEVLGLYGLIVALLL 151
>SPAC732.01 |vma11||V-type ATPase proteolipid
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 162
Score = 196 bits (479), Expect = 2e-51
Identities = 91/147 (61%), Positives = 116/147 (78%)
Frame = +3
Query: 78 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 257
PIY FFG G ++++FS LGA YGTA +G GIAA+ RPE++MKS+IPVVM+GII +
Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66
Query: 258 YGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQP 437
YGLV++VLIAG + +Y L+ GFIHL AGLAVG +G+AAG+AIG+VGD GV+ +Q
Sbjct: 67 YGLVMSVLIAGDMSPDNDYSLFSGFIHLSAGLAVGLTGVAAGYAIGVVGDRGVQSFMRQD 126
Query: 438 RLFVGMILILIFAEVLGLYGLIVAIYL 518
R+FV M+LILIFAEVLGLYGLIV + L
Sbjct: 127 RIFVSMVLILIFAEVLGLYGLIVGLIL 153
>SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 199
Score = 98.3 bits (234), Expect = 8e-22
Identities = 49/147 (33%), Positives = 81/147 (55%), Gaps = 6/147 (4%)
Frame = +3
Query: 96 FGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVA 275
+G++G AS + F +GAA+G GT I +V P + K++I ++ ++AIY L++A
Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIA 104
Query: 276 VLIAGALQE--PANY----PLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQP 437
++ + + + PA + Y GF G+ VG L G +GI G + AQ
Sbjct: 105 IVFSAKINDINPAGFYTKSHYYTGFALFWGGITVGLCNLICGVCVGITGSSAALADAQDA 164
Query: 438 RLFVGMILILIFAEVLGLYGLIVAIYL 518
LFV ++++ IF VLGL+GLIV + +
Sbjct: 165 SLFVKVLVVEIFGSVLGLFGLIVGLLI 191
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.5 bits (68), Expect = 0.10
Identities = 20/57 (35%), Positives = 28/57 (49%)
Frame = -2
Query: 496 P*RPNTSAKIRIRIIPTNNLGC*AVPRTPASPTMPMAKPAARPENPTAKPAPKWMNP 326
P RP ++A ++ PT +VP P++P MP P+A P A AP NP
Sbjct: 1691 PVRPQSAAPPQMSA-PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPAC212.04c |||S. pombe specific DUF999 family protein
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 288
Score = 31.1 bits (67), Expect = 0.13
Identities = 21/68 (30%), Positives = 30/68 (44%)
Frame = +3
Query: 222 IIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIV 401
II +AG+IA + +++ IAG + G ++ G L LA GF I
Sbjct: 193 IITATIAGVIAAFSVIITATIAGVIAAMV------GILYFGHWLVYKILILAFGFKIVTS 246
Query: 402 GDAGVRGT 425
GD V T
Sbjct: 247 GDVCVSNT 254
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 28.3 bits (60), Expect = 0.95
Identities = 26/65 (40%), Positives = 30/65 (46%), Gaps = 2/65 (3%)
Frame = -2
Query: 427 AVPRTPAS--PTMPMAKPAARPENPTAKPAPKWMNPL*RG*LAGSWRAPAIRTATTRP*M 254
A P P S P++PM PAA P P+A AP PL AG AP + A P
Sbjct: 427 APPSLPPSAPPSLPMGAPAAPPLPPSAPIAP----PL----PAGMPAAPPLPPAAPAPPP 478
Query: 253 AIIPA 239
A PA
Sbjct: 479 APAPA 483
>SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 281
Score = 27.9 bits (59), Expect = 1.3
Identities = 17/39 (43%), Positives = 25/39 (64%), Gaps = 3/39 (7%)
Frame = -1
Query: 524 CVQVDGDD-KSV--KTQYFSENKNKNHSDE*PRLLSSTT 417
C++VD +D K + K+QY +EN N N + P L S+TT
Sbjct: 233 CIEVDSEDWKDLVWKSQYATENANTNSINNSP-LSSNTT 270
>SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr
1|||Manual
Length = 249
Score = 27.5 bits (58), Expect = 1.7
Identities = 22/72 (30%), Positives = 32/72 (44%)
Frame = +3
Query: 81 IYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIY 260
+YGPF+ A+ FS Y +G G + S+ K +I A II Y
Sbjct: 87 LYGPFWITTTVIQALFFSNSITEYARYATGHGTSGYSI-------KKLISA--ASIIYGY 137
Query: 261 GLVVAVLIAGAL 296
++AVL+ G L
Sbjct: 138 TTIIAVLLWGIL 149
>SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic
subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1729
Score = 27.1 bits (57), Expect = 2.2
Identities = 21/95 (22%), Positives = 47/95 (49%), Gaps = 6/95 (6%)
Frame = +3
Query: 57 NKMAENNP---IYGPFFG-VMGAASAIIFSALGAAYGTAKSGTGIAAMSVMR-PELIMKS 221
+K+A ++P I F+G ++G + F+ + + ++ G S LI+ S
Sbjct: 1424 DKIAMDSPRARITTMFYGEILGPLGTLFFTCIPFLFINSQPGNDDETQSTNAFIRLIIMS 1483
Query: 222 IIPVVMAGIIAIYGLVVAVLIAGALQEPA-NYPLY 323
+ P+V++ IIA + + +++ L + + Y +Y
Sbjct: 1484 VAPLVLSAIIAFFFFCLGIMLRPILGDRSKTYGVY 1518
>SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 760
Score = 26.2 bits (55), Expect = 3.8
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -1
Query: 335 DEPFVKGVVGWLLEGTSNQDSHDQTVDGNNTRHDDRND 222
DEPF+K +L+ SN++S+ VD N+ D +D
Sbjct: 48 DEPFLKSKYMDILQKISNRESNVINVDLNDLYEFDPSD 85
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 25.4 bits (53), Expect = 6.7
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 4/34 (11%)
Frame = -2
Query: 427 AVPRTPASPTMPMA----KPAARPENPTAKPAPK 338
+VP+ PA+P +P A +P A P P AP+
Sbjct: 513 SVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQ 546
>SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large
subunit Rpc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1405
Score = 25.0 bits (52), Expect = 8.8
Identities = 8/19 (42%), Positives = 13/19 (68%)
Frame = -3
Query: 402 PRCLWRNRQPDQRILQPNQ 346
PRCLW +Q +++PN+
Sbjct: 582 PRCLWTGKQVFTVLMKPNR 600
>SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 535
Score = 25.0 bits (52), Expect = 8.8
Identities = 8/11 (72%), Positives = 9/11 (81%)
Frame = -3
Query: 36 TGPKNCDDYLH 4
TGP N DDY+H
Sbjct: 369 TGPSNTDDYIH 379
>SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit
Bgs2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1894
Score = 25.0 bits (52), Expect = 8.8
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = +1
Query: 481 YWVFTDLSSPSTCTQNKRPEHTPLPSP 561
YW +T +SP+ +N R P P P
Sbjct: 497 YWYYTVFNSPTIIEKNFRQSVGPKPIP 523
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,716,958
Number of Sequences: 5004
Number of extensions: 56769
Number of successful extensions: 196
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 273658928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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