Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0120
(658 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g14185.1 68414.m01678 glucose-methanol-choline (GMC) oxidored... 64 1e-10
At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative / (R... 62 3e-10
At1g14190.1 68414.m01679 glucose-methanol-choline (GMC) oxidored... 61 6e-10
At1g72970.1 68414.m08439 glucose-methanol-choline (GMC) oxidored... 56 2e-08
At5g51930.1 68418.m06442 glucose-methanol-choline (GMC) oxidored... 56 3e-08
At5g51950.1 68418.m06447 glucose-methanol-choline (GMC) oxidored... 55 4e-08
At1g12570.1 68414.m01459 glucose-methanol-choline (GMC) oxidored... 53 1e-07
At3g56060.1 68416.m06229 glucose-methanol-choline (GMC) oxidored... 52 3e-07
At4g19380.1 68417.m02853 alcohol oxidase-related similar to long... 35 0.041
At4g24610.1 68417.m03525 expressed protein 29 2.1
At4g39050.1 68417.m05531 kinesin-related protein (MKRP2) kinesin... 28 4.8
At2g29990.1 68415.m03648 pyridine nucleotide-disulphide oxidored... 28 4.8
At2g21380.1 68415.m02544 kinesin motor protein-related 28 4.8
At3g54210.1 68416.m05992 ribosomal protein L17 family protein co... 28 6.3
At3g42600.1 68416.m04422 hypothetical protein 28 6.3
>At1g14185.1 68414.m01678 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 503
Score = 63.7 bits (148), Expect = 1e-10
Identities = 48/135 (35%), Positives = 71/135 (52%), Gaps = 8/135 (5%)
Frame = -2
Query: 387 FLEAGRIFGHPTIDYNSPHQLGFGYVQAITTK-GHRQSAAKVFLHRQKKRQNLHILPDTY 211
FLEAG +P Y+ H G +I + G R ++A + + K + +L +
Sbjct: 170 FLEAGF---YPYNGYSLEHTQGTKIGGSIFDQCGKRHTSADLLGYG--KPNCITVLLNAT 224
Query: 210 VTKILIDARTKTAYGVEYVR---NRLK----HIVLARKEVILSAGPIASPQLLMLSGVGP 52
V I+ DA A GV ++ N K H+ R EVIL+AG + SPQ+L+LSG+GP
Sbjct: 225 VKSIIFDANKTRAVGVRFMESDGNSSKSYKVHVEQHRGEVILAAGALGSPQILLLSGIGP 284
Query: 51 KKHLNSMGIPVLKDL 7
+ HLN IPV+ +L
Sbjct: 285 ENHLNDFDIPVIVNL 299
>At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative /
(R)-oxynitrilase, putative similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|52707];
contains Pfam protile PF00732 GMC oxidoreductase
Length = 552
Score = 62.1 bits (144), Expect = 3e-10
Identities = 48/146 (32%), Positives = 73/146 (50%), Gaps = 10/146 (6%)
Frame = -2
Query: 411 FRTKLIKAFLEAGRIFGHPTIDYNSPHQLGFGYVQAITTK-GHRQSAAKVFLHRQKKRQN 235
++T + A LE G HP + H++G + + G R S+A + R + N
Sbjct: 181 WQTAIRDALLEVGV---HPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLL--RYARSSN 235
Query: 234 LHILPDTYVTKIL------IDARTKTAYGVEY--VRNRLKHIVLA-RKEVILSAGPIASP 82
+ + V ++L + +A GV Y R H ++ R EVILSAG + SP
Sbjct: 236 IRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSP 295
Query: 81 QLLMLSGVGPKKHLNSMGIPVLKDLP 4
QLL LSG+GP+ +L++ GIPV D P
Sbjct: 296 QLLFLSGIGPRSYLSTWGIPVALDQP 321
>At1g14190.1 68414.m01679 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 501
Score = 61.3 bits (142), Expect = 6e-10
Identities = 49/136 (36%), Positives = 72/136 (52%), Gaps = 9/136 (6%)
Frame = -2
Query: 387 FLEAGRIFGHPTIDYNSPHQLGFGYVQAITTK-GHRQSAAKVFLHRQKKRQNLHILPDTY 211
FLEAG +P Y+ H G +I + G R ++A + K + +L +T
Sbjct: 149 FLEAGF---YPYNGYSLEHTQGTKIGGSIYDQCGKRHTSADLLGFG--KPNCITVLLNTT 203
Query: 210 VTKILIDARTKT-AYGVEYVR---NRLK----HIVLARKEVILSAGPIASPQLLMLSGVG 55
V I+ D+ KT A GV ++ N K H+ R EVIL+AG + SPQ+L+LSG+G
Sbjct: 204 VKSIIFDSSNKTRAVGVRFMESDGNSSKSYKVHVEQHRGEVILAAGALGSPQILLLSGIG 263
Query: 54 PKKHLNSMGIPVLKDL 7
P+ HL IPV+ +L
Sbjct: 264 PENHLKDFDIPVIVNL 279
>At1g72970.1 68414.m08439 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 594
Score = 56.0 bits (129), Expect = 2e-08
Identities = 49/141 (34%), Positives = 73/141 (51%), Gaps = 8/141 (5%)
Frame = -2
Query: 414 IFRTKLIKAFLEAG-RIFGHPTIDYNSPHQLGFGYVQAITTKGHRQSAAKVFLHRQKKRQ 238
+++ L + LE G R F T D+ S ++G G + G R +AA++ + Q
Sbjct: 186 LWQKALRDSLLEVGVRPFNGFTYDHVSGTKIG-GTI--FDRFGRRHTAAELLAYANP--Q 240
Query: 237 NLHILPDTYVTKILIDA-----RTKTAYGVEYVRNRLKHIVLARK--EVILSAGPIASPQ 79
L +L V KI+ D R + N+ + ++ RK EVILS+G I SPQ
Sbjct: 241 KLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSPQ 300
Query: 78 LLMLSGVGPKKHLNSMGIPVL 16
+LMLSG+GPKK L + IPV+
Sbjct: 301 MLMLSGIGPKKELQRLKIPVV 321
>At5g51930.1 68418.m06442 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 582
Score = 55.6 bits (128), Expect = 3e-08
Identities = 34/98 (34%), Positives = 50/98 (51%), Gaps = 1/98 (1%)
Frame = -2
Query: 291 GHRQSAAKVFLHRQKKRQNLHILPDTYVTKILIDARTKTAYGVEYVRNRLKHIVLARKEV 112
GHR +AA + + R +++ + +A V ++ A EV
Sbjct: 235 GHRHTAANLLEYANPNRIVVYLHASVHKVLFTTEAYEVLFEDANGVFHKANLANKATNEV 294
Query: 111 ILSAGPIASPQLLMLSGVGPKKHLNSMGI-PVLKDLPV 1
ILSAG + SPQLLMLSGVGP HL + G+ P++ D P+
Sbjct: 295 ILSAGALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQPM 332
>At5g51950.1 68418.m06447 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 586
Score = 55.2 bits (127), Expect = 4e-08
Identities = 43/106 (40%), Positives = 57/106 (53%), Gaps = 9/106 (8%)
Frame = -2
Query: 291 GHRQSAAKVFLHRQKKRQNLHILPDTYVTKILIDARTKT---AYGVEYV-RNRLKHIVLA 124
GHR +AA + + N+ + V KIL + + AYGV + N + H
Sbjct: 208 GHRHTAADLLEYANPG--NIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAEL 265
Query: 123 RK----EVILSAGPIASPQLLMLSGVGPKKHLNSMGI-PVLKDLPV 1
K EVILSAG I SPQLLMLSG+GP HL + GI P++ D P+
Sbjct: 266 EKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPM 311
>At1g12570.1 68414.m01459 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 572
Score = 53.2 bits (122), Expect = 1e-07
Identities = 49/145 (33%), Positives = 69/145 (47%), Gaps = 9/145 (6%)
Frame = -2
Query: 411 FRTKLIKAFLEAGRIFGHPTIDYNSPHQLGFGYVQAITTK-GHRQSAAKVFLHRQKKRQN 235
++T + LEAG + P + H G + I + G+R +AA + + K
Sbjct: 170 WQTAVRDGLLEAGIV---PNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPK--G 224
Query: 234 LHILPDTYVTKILIDARTKT---AYGVEYV-RNRLKHIVLARK----EVILSAGPIASPQ 79
+ +L V +IL R T A GV Y R H ++ E+ILSAG + SPQ
Sbjct: 225 ITVLLHATVHRILFRTRGTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQ 284
Query: 78 LLMLSGVGPKKHLNSMGIPVLKDLP 4
LLMLSGVGP L + I V+ D P
Sbjct: 285 LLMLSGVGPSAQLQAQNITVVMDQP 309
>At3g56060.1 68416.m06229 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 577
Score = 52.0 bits (119), Expect = 3e-07
Identities = 27/40 (67%), Positives = 31/40 (77%), Gaps = 1/40 (2%)
Frame = -2
Query: 117 EVILSAGPIASPQLLMLSGVGPKKHLNSMGI-PVLKDLPV 1
EVILSAG IASPQLLMLSGVGP HL + + PV+ D P+
Sbjct: 274 EVILSAGAIASPQLLMLSGVGPAAHLAAYRVNPVIVDQPM 313
>At4g19380.1 68417.m02853 alcohol oxidase-related similar to long
chain fatty alcohol oxidase from Candida cloacae
[GI:6983581], Candida tropicalis [GI:6983594]
Length = 726
Score = 35.1 bits (77), Expect = 0.041
Identities = 27/101 (26%), Positives = 47/101 (46%), Gaps = 3/101 (2%)
Frame = -2
Query: 351 IDYNSPHQLGFGYVQAITTKGHRQSAAKVFLHRQKKRQNLHILPDTYVTKILID---ART 181
I N+P G+ KG +Q ++ +L + N ILP T+++ D +
Sbjct: 373 IPRNAPSDHYCGFCCLGCKKGQKQGTSETWLVDLVESDNGLILPGCQATEVMYDCEQGKK 432
Query: 180 KTAYGVEYVRNRLKHIVLARKEVILSAGPIASPQLLMLSGV 58
K A GV + ++V +R I++ G + +P LL SG+
Sbjct: 433 KKATGVAFAFGEEIYVVESR-VTIVACGALRTPHLLKRSGL 472
>At4g24610.1 68417.m03525 expressed protein
Length = 1145
Score = 29.5 bits (63), Expect = 2.1
Identities = 21/71 (29%), Positives = 39/71 (54%), Gaps = 6/71 (8%)
Frame = -2
Query: 486 YKQINLTISNFEIYIPRIFVYLFIIFR--TKLIKAFLEA-GRIFG---HPTIDYNSPHQL 325
YK++N TI ++++ I R Y+F++ + KA +EA + + P + +P +L
Sbjct: 859 YKRLNETIQDYQVIISRWPEYIFVLESAIADVEKATVEALEKQYADVLSPLKENLAPKKL 918
Query: 324 GFGYVQAITTK 292
F YVQ +T +
Sbjct: 919 SFKYVQKLTKR 929
>At4g39050.1 68417.m05531 kinesin-related protein (MKRP2) kinesin
motor protein - Ustilago maydis, PID:g2062750; identical
to cDNA MKRP2 mRNA for kinesin-related protein
GI:16902293, kinesin-related protein [Arabidopsis
thaliana] GI:16902294
Length = 1055
Score = 28.3 bits (60), Expect = 4.8
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = +2
Query: 239 CRFFCLCRNTFAALCRCPL 295
CR FCLC++ A CP+
Sbjct: 1023 CRHFCLCKSCSLACSECPI 1041
>At2g29990.1 68415.m03648 pyridine nucleotide-disulphide
oxidoreductase family protein similar to SP|P32340
Rotenone-insensitive NADH-ubiquinone oxidoreductase,
mitochondrial precursor (EC 1.6.5.3) (Internal NADH
dehydrogenase) {Saccharomyces cerevisiae} ; contains
Pfam profile PF00070: Pyridine nucleotide-disulphide
oxidoreductase
Length = 508
Score = 28.3 bits (60), Expect = 4.8
Identities = 18/50 (36%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
Frame = -2
Query: 168 GVEYVRNRLKHIVLARKEVILSAGPIASPQLLMLS-GVGPKKHLNSMGIP 22
GV +VR +K + +++IL G LL+ S GVGP + S+G+P
Sbjct: 307 GVRFVRGIVKDV--QSQKLILDDGTEVPYGLLVWSTGVGPSPFVRSLGLP 354
>At2g21380.1 68415.m02544 kinesin motor protein-related
Length = 1058
Score = 28.3 bits (60), Expect = 4.8
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = +2
Query: 239 CRFFCLCRNTFAALCRCPL 295
CR FCLC++ A CP+
Sbjct: 1026 CRHFCLCKSCSLACSECPI 1044
>At3g54210.1 68416.m05992 ribosomal protein L17 family protein
contains Pfam profile: PF01196 ribosomal protein L17
Length = 211
Score = 27.9 bits (59), Expect = 6.3
Identities = 15/35 (42%), Positives = 21/35 (60%)
Frame = -2
Query: 126 ARKEVILSAGPIASPQLLMLSGVGPKKHLNSMGIP 22
+R+ V L PI+SP L SG+ P L S+G+P
Sbjct: 42 SRRPVTLRL-PISSPSLPSFSGLSPVNPLLSIGLP 75
>At3g42600.1 68416.m04422 hypothetical protein
Length = 219
Score = 27.9 bits (59), Expect = 6.3
Identities = 17/61 (27%), Positives = 31/61 (50%), Gaps = 4/61 (6%)
Frame = +3
Query: 210 RMCPARCVSFVASSVYAGILSQHFVGVLWLL*LAHNRILIGAV----NCNQLLDVQRFYR 377
R PA+ + +AS + I +++GV++L A + +G +C Q +D + YR
Sbjct: 105 RHAPAQADATLASGILFIIDGNYYLGVMFLQLFARHHYPLGTEETRDSCEQFMDEIKQYR 164
Query: 378 P 380
P
Sbjct: 165 P 165
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,298,161
Number of Sequences: 28952
Number of extensions: 294863
Number of successful extensions: 597
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 581
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 596
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1373722560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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