Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0080
         (324 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    55   2e-09
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    55   2e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    45   2e-06
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    31   0.033
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi...    29   0.17 
SPAC23C11.01 |||ER membrane protein, ICE2 family|Schizosaccharom...    27   0.70 
SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizos...    25   2.1  
SPAC26H5.02c |||DNA replication ATPase|Schizosaccharomyces pombe...    25   3.7  
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    24   4.9  
SPBC337.14 |rpb4||DNA-directed RNA polymerase II complex subunit...    24   4.9  
SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr 2|||Ma...    24   6.5  
SPAC6F6.13c |||DUF726 family protein|Schizosaccharomyces pombe|c...    24   6.5  
SPBC800.10c |||EPS15 repeat family actin cortical patch componen...    24   6.5  
SPBC887.09c |||leucine-rich repeat protein Sog2 |Schizosaccharom...    24   6.5  
SPBC25B2.07c |mug164||microtubule-associated protein|Schizosacch...    23   8.6  
SPBC582.10c |||ATP-dependent DNA helicase Rhp16b |Schizosaccharo...    23   8.6  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 55.2 bits (127), Expect = 2e-09
 Identities = 26/92 (28%), Positives = 57/92 (61%)
 Frame = +3

Query: 48  EQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINF 227
           ++++A  R+AF +FD  + G I   ++  ++ ++GQ    +ELQ +I+E D + +G I+F
Sbjct: 8   DEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDF 67

Query: 228 DGFCNIASISLKRRR*SHAQELKEAFTLYDRE 323
             F  +  ++ K +   + +E++EAF ++D++
Sbjct: 68  TEF--LTMMARKMKDTDNEEEVREAFKVFDKD 97



 Score = 50.4 bits (115), Expect = 7e-08
 Identities = 23/72 (31%), Positives = 45/72 (62%)
 Frame = +3

Query: 36  DSDDEQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSG 215
           D+D+E+++   R+AF++FD   +GYI V +++ +L ++G+     E+  +I E D +  G
Sbjct: 80  DTDNEEEV---REAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDG 136

Query: 216 KINFDGFCNIAS 251
            IN++ F  + S
Sbjct: 137 VINYEEFSRVIS 148


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 55.2 bits (127), Expect = 2e-09
 Identities = 22/55 (40%), Positives = 35/55 (63%)
 Frame = +3

Query: 72  KAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGF 236
           KAF++FD   SGYI+  K +  + T+G+   D+E+Q ++ E DP NSG  ++  F
Sbjct: 82  KAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDF 136


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 45.2 bits (102), Expect = 2e-06
 Identities = 19/60 (31%), Positives = 36/60 (60%)
 Frame = +3

Query: 66  LRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNI 245
           +++AF++FD  ++G I +  +  +   + +  DD EL+A+I+E D +  G+IN   F  I
Sbjct: 112 IKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFIAI 171


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 31.5 bits (68), Expect = 0.033
 Identities = 16/58 (27%), Positives = 29/58 (50%)
 Frame = +3

Query: 72  KAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFCNI 245
           +AF +FD T  GYID   +      +G+     +LQ ++D      +GK++ + F  +
Sbjct: 16  EAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDL--AGTNGKVSREEFAEL 71


>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
           Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 190

 Score = 29.1 bits (62), Expect = 0.17
 Identities = 18/68 (26%), Positives = 29/68 (42%)
 Frame = +3

Query: 60  AMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFDGFC 239
           A     F +FD  K+GYID  +    L+   +   + +L       D +N+G I++D   
Sbjct: 63  AFAEYVFNVFDADKNGYIDFKEFICALSVTSRGELNDKLIWAFQLYDLDNNGLISYDEML 122

Query: 240 NIASISLK 263
            I     K
Sbjct: 123 RIVDAIYK 130


>SPAC23C11.01 |||ER membrane protein, ICE2
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 441

 Score = 27.1 bits (57), Expect = 0.70
 Identities = 13/33 (39%), Positives = 21/33 (63%)
 Frame = +3

Query: 120 LKISTILNTMGQLFDDSELQALIDENDPENSGK 218
           + +ST+ +T  +  DDS L+ +ID+  PE  GK
Sbjct: 12  ISVSTVEDTSFEAMDDSILE-IIDDEAPEIQGK 43


>SPAC694.06c |mrc1||mediator of replication checkpoint 1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1019

 Score = 25.4 bits (53), Expect = 2.1
 Identities = 14/44 (31%), Positives = 26/44 (59%)
 Frame = +3

Query: 186 IDENDPENSGKINFDGFCNIASISLKRRR*SHAQELKEAFTLYD 317
           I E+D E++G +  D F  ++S S+K  +    +EL ++F + D
Sbjct: 545 ISESDDESNGDMIRDSFDRLSSESIKDSQ--KTEELHDSFGIND 586


>SPAC26H5.02c |||DNA replication ATPase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 504

 Score = 24.6 bits (51), Expect = 3.7
 Identities = 10/27 (37%), Positives = 18/27 (66%)
 Frame = +3

Query: 93  TTKSGYIDVLKISTILNTMGQLFDDSE 173
           TTKS +I++   ST +    ++F+DS+
Sbjct: 143 TTKSRFIEISATSTTVADCRKIFEDSQ 169


>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 24.2 bits (50), Expect = 4.9
 Identities = 10/16 (62%), Positives = 12/16 (75%)
 Frame = +3

Query: 276 SHAQELKEAFTLYDRE 323
           S  QELKEAF L D++
Sbjct: 45  SQIQELKEAFALLDKD 60


>SPBC337.14 |rpb4||DNA-directed RNA polymerase II complex subunit
           Rpb4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 135

 Score = 24.2 bits (50), Expect = 4.9
 Identities = 12/47 (25%), Positives = 23/47 (48%), Gaps = 3/47 (6%)
 Frame = +3

Query: 63  MLRKAFQMFDTTKSGYI---DVLKISTILNTMGQLFDDSELQALIDE 194
           +L   F  F+  + G +   D  +  T++ ++    DD  LQ ++DE
Sbjct: 80  ILGNRFHKFERAQLGTLCCEDAEEARTLIPSLANKIDDQNLQGILDE 126


>SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 918

 Score = 23.8 bits (49), Expect = 6.5
 Identities = 12/36 (33%), Positives = 18/36 (50%)
 Frame = +3

Query: 147 MGQLFDDSELQALIDENDPENSGKINFDGFCNIASI 254
           M  L DDS L  ++ ++  +N G    D F  I S+
Sbjct: 270 MMDLLDDSTLLIVMGDHGMDNKGNHGGDSFDEINSV 305


>SPAC6F6.13c |||DUF726 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 778

 Score = 23.8 bits (49), Expect = 6.5
 Identities = 12/49 (24%), Positives = 24/49 (48%)
 Frame = +3

Query: 36  DSDDEQKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQA 182
           D DD+QK  +  +A ++ D    G  + + + + L   G +  ++E  A
Sbjct: 109 DEDDDQKHQLDSEAIKLLDIADHGN-EEISMDSQLEITGNILSETEKMA 156


>SPBC800.10c |||EPS15 repeat family actin cortical patch component
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1116

 Score = 23.8 bits (49), Expect = 6.5
 Identities = 16/72 (22%), Positives = 30/72 (41%), Gaps = 1/72 (1%)
 Frame = +3

Query: 51  QKMAMLRKAFQMFDTTKSGYIDVLKISTILNTMGQLFDDSELQALIDENDPENSGKINFD 230
           Q +    + F   D    GY+   +  +    +     +  L  + D +D  ++GK+N  
Sbjct: 258 QDLTSFCQLFSNVDKAHKGYVSGSEAYSFF--LASKLPEDVLAQIWDLSDTNSNGKLNIG 315

Query: 231 GFC-NIASISLK 263
            FC ++  I LK
Sbjct: 316 EFCISLYLIKLK 327


>SPBC887.09c |||leucine-rich repeat protein Sog2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 886

 Score = 23.8 bits (49), Expect = 6.5
 Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 3/29 (10%)
 Frame = -3

Query: 100 FVVSNIWNALRSMAIFCSSS---ESMLHV 23
           F +S +  ALR   +FCS+    +SM HV
Sbjct: 515 FSLSQVQQALRQQLLFCSNPVVLDSMRHV 543


>SPBC25B2.07c |mug164||microtubule-associated
          protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 501

 Score = 23.4 bits (48), Expect = 8.6
 Identities = 11/22 (50%), Positives = 13/22 (59%)
 Frame = -3

Query: 91 SNIWNALRSMAIFCSSSESMLH 26
          SNI   L S +IF  S+ S LH
Sbjct: 13 SNILKELHSTSIFTDSTTSQLH 34


>SPBC582.10c |||ATP-dependent DNA helicase Rhp16b
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 830

 Score = 23.4 bits (48), Expect = 8.6
 Identities = 14/41 (34%), Positives = 22/41 (53%)
 Frame = -2

Query: 128 DLQHVDVTRLRGVKHLECFTQHGHLLFIIGIHVARQGLAAV 6
           D Q  D T +RG++    F+Q    L II +H+ + G+  V
Sbjct: 659 DEQEEDDT-VRGLRKTIIFSQFTTFLDIIDLHLRKAGIGFV 698


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,430,790
Number of Sequences: 5004
Number of extensions: 27077
Number of successful extensions: 100
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 93
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 99
length of database: 2,362,478
effective HSP length: 64
effective length of database: 2,042,222
effective search space used: 87815546
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)