Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0070
(638 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 222 3e-59
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 136 3e-33
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 134 1e-32
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 128 9e-31
SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 111 8e-26
SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 96 4e-21
SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc... 93 4e-20
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 32 0.080
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 31 0.19
SPBC776.06c |||spindle pole body interacting protein |Schizosacc... 27 2.3
SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomy... 26 5.3
SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit Pa... 25 9.2
SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst... 25 9.2
SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc... 25 9.2
>SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 259
Score = 222 bits (543), Expect = 3e-59
Identities = 102/175 (58%), Positives = 134/175 (76%), Gaps = 1/175 (0%)
Frame = +2
Query: 92 YDRAITVFSPDGHLLQVEYAQEAVRKGSTAXXXXXXXXXXXXXEKKSVAKLQEERTVRKI 271
YDRA++VFSPDG LLQVEY QEAVR+G+TA E+K+V KLQ +KI
Sbjct: 4 YDRALSVFSPDGRLLQVEYGQEAVRRGTTAIALRGNECIVIGVERKNVPKLQNVSNFQKI 63
Query: 272 CLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLKQKYTQSN 451
++D+HV +AFAGL ADARILI++A++E Q+HKL + DPV++EY+TRY+AG++QKYTQS
Sbjct: 64 AMVDNHVCLAFAGLNADARILIDKARVEAQNHKLNLADPVSIEYLTRYVAGVQQKYTQSG 123
Query: 452 GRRPFGISCLIGGFDY-DGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEKNY 613
G RPFG+S LI GFD D +P ++QTEP+GIY WKA A GR++K RE+LEKN+
Sbjct: 124 GVRPFGVSTLIAGFDVGDNTPRVYQTEPAGIYNAWKATAIGRASKAAREYLEKNW 178
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 136 bits (328), Expect = 3e-33
Identities = 72/182 (39%), Positives = 102/182 (56%), Gaps = 1/182 (0%)
Frame = +2
Query: 80 MSARYDRAITVFSPDGHLLQVEYAQEAVRKGSTAXXXXXXXXXXXXXEKKSVAKL-QEER 256
MS YD T+FSP+G L QVEYA EA+ A EKK +KL ++E
Sbjct: 1 MSRSYDSRTTIFSPEGRLYQVEYALEAINHAGVALGIVAKDGIVLAAEKKVTSKLLEQEE 60
Query: 257 TVRKICLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLKQK 436
+ K+ + D+++ A AGLTADA ILIN A+ Q + T + + E + R + LKQ
Sbjct: 61 SAEKLYHIGDNMLCAVAGLTADANILINYARRVGQQYLQTFNEEMPCEQLVRRVCDLKQG 120
Query: 437 YTQSNGRRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEKNYT 616
YTQ G RPFG+S L G+D+ LFQ+ PSG Y W+AN+ G ++ +V+ + + Y
Sbjct: 121 YTQYGGLRPFGVSFLYAGWDHIRGYQLFQSNPSGNYGSWQANSIGGNSTSVQSLMRQEYK 180
Query: 617 AD 622
D
Sbjct: 181 DD 182
>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 134 bits (324), Expect = 1e-32
Identities = 71/195 (36%), Positives = 107/195 (54%), Gaps = 6/195 (3%)
Frame = +2
Query: 71 FLTMSARYDRAITVFSPDGHLLQVEYAQEAVRKGSTAXXXXXXXXXXXXXEKKSVAKLQE 250
F+T S YDR + FSP+G L QVEYA EA++ GSTA EK+ + L E
Sbjct: 2 FMTRS-EYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKDAVVLGVEKRLTSPLME 60
Query: 251 ERTVRKICLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLK 430
+V K+ +D H+ A +GLTADAR +I A+++ Q+H+ T ++P +E T+ I L
Sbjct: 61 SHSVEKLFEIDSHIGCAISGLTADARTIIEHARVQTQNHRFTYDEPQGIESTTQSICDLA 120
Query: 431 QKYTQSNG------RRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVR 592
++ + RPFG++ LI G D G P L+ +EPSG Y+ ++A A G ++ +
Sbjct: 121 LRFGEGEDGEERIMSRPFGVALLIAGIDEHG-PQLYHSEPSGTYFRYEAKAIGSGSEPAK 179
Query: 593 EFLEKNYTADEVATE 637
L K + D E
Sbjct: 180 SELVKEFHKDMTLEE 194
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 128 bits (308), Expect = 9e-31
Identities = 68/185 (36%), Positives = 108/185 (58%), Gaps = 2/185 (1%)
Frame = +2
Query: 80 MSARYDRAITVFSPDGHLLQVEYAQEAVRKGSTAXXXXXXXXXXXXXEKKSVAKLQEERT 259
M+ +Y ++T FSP+G L+Q+EYA AV G T+ EKK ++L +
Sbjct: 1 MTDKYAFSLTTFSPNGKLVQIEYALNAVNAGVTSVGIKATDGVVLATEKKPTSELAIGAS 60
Query: 260 VRKICLLDDHVVMAFAGLTADARILINRAQIECQS--HKLTVEDPVTLEYITRYIAGLKQ 433
+ K+C + + M ++G+ D R+L+++++ Q+ K+ E P T + + + IA + Q
Sbjct: 61 LEKVCAITPDIGMVYSGMGPDFRVLVDKSRKVAQTTYKKIYNEYPPT-KILVQEIASVMQ 119
Query: 434 KYTQSNGRRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEKNY 613
+ TQS G RPFG+S L+ G D G P L+Q +PSG Y+ WKA A G+S+ + FLEK Y
Sbjct: 120 ESTQSGGVRPFGVSLLVAGMDEKG-PSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEKRY 178
Query: 614 TADEV 628
DE+
Sbjct: 179 N-DEL 182
>SPAC6G10.04c |||20S proteasome component alpha 6 subunit
Pre5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 111 bits (267), Expect = 8e-26
Identities = 59/174 (33%), Positives = 94/174 (54%)
Frame = +2
Query: 89 RYDRAITVFSPDGHLLQVEYAQEAVRKGSTAXXXXXXXXXXXXXEKKSVAKLQEERTVRK 268
+YD T +SP G L QVEYA EA+++GS K++ +L + +K
Sbjct: 5 QYDGDATTWSPQGRLHQVEYALEAIKQGSATVGLVSKTHAVLVALKRNAEELSSYQ--KK 62
Query: 269 ICLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLKQKYTQS 448
+ +DDH+ +A AGL DAR+L N + E S K P+ + + +A Q TQ
Sbjct: 63 LIRIDDHIGIAIAGLAPDARVLSNYMKQEALSSKTLFTRPIPVRRLMSKVAEKAQINTQE 122
Query: 449 NGRRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEKN 610
GRRP+G+ L+ G+D G PHL + +PSG+ E+ + G +++ R ++E+N
Sbjct: 123 YGRRPYGVGFLVIGYDESG-PHLLEFQPSGLVLEYLGTSMGSRSQSARTYIERN 175
>SPCC1795.04c |||20S proteasome component alpha
7|Schizosaccharomyces pombe|chr 3|||Manual
Length = 253
Score = 95.9 bits (228), Expect = 4e-21
Identities = 54/172 (31%), Positives = 82/172 (47%)
Frame = +2
Query: 92 YDRAITVFSPDGHLLQVEYAQEAVRKGSTAXXXXXXXXXXXXXEKKSVAKLQEERTVRKI 271
YD + FSPDG L Q EYA +AV ST EK +KL + R +I
Sbjct: 8 YDLGL-FFSPDGRLFQAEYAYKAVENASTCIGIKCEDGVILALEKVVTSKLLKPRVNNRI 66
Query: 272 CLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLKQKYTQSN 451
+D H+ +A G D + ++ RA+ E S + P+ I + Q +T +
Sbjct: 67 GSVDRHIGIATTGFIPDGQHIVKRARDEATSWRDNYGSPIPGTVIADRLGNYVQLFTCYS 126
Query: 452 GRRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEK 607
RPFG+ + +D +G PHL+ EP+G+Y+ + A G+ + R LEK
Sbjct: 127 SVRPFGVMSFVATYDSEG-PHLYMVEPNGVYWGYNGAAAGKGRQVARNELEK 177
>SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 244
Score = 92.7 bits (220), Expect = 4e-20
Identities = 54/186 (29%), Positives = 89/186 (47%), Gaps = 1/186 (0%)
Frame = +2
Query: 74 LTMSARYDRAITVFSPDGHLLQVEYAQEAVRK-GSTAXXXXXXXXXXXXXEKKSVAKLQE 250
++ + +DR ITVFSP+G L QVEYA +A G T+ +KK KL +
Sbjct: 1 MSQARGFDRTITVFSPEGRLYQVEYAFKAFNNAGITSVGVTGKNCACVISQKKVPDKLID 60
Query: 251 ERTVRKICLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLK 430
TV+ + + + G ADAR ++RA+ E + P+ + + + +A +
Sbjct: 61 ASTVKHVFPITKGIGCVMTGSIADARAQVSRARSEAAEFEYKNGYPMPCDVLAKRMANIA 120
Query: 431 QKYTQSNGRRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEKN 610
Q TQ RP G++ + D + P LF+ +P+G Y +KA + G +LEK
Sbjct: 121 QVSTQRAAMRPLGVAMTLVAVDDEIGPSLFKLDPAGFYIGYKATSAGPKQTETINWLEKR 180
Query: 611 YTADEV 628
+ D +
Sbjct: 181 FKKDGI 186
>SPAC22F8.06 |pam1||20S proteasome component beta
6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 225
Score = 31.9 bits (69), Expect = 0.080
Identities = 25/109 (22%), Positives = 45/109 (41%), Gaps = 2/109 (1%)
Frame = +2
Query: 284 DHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLKQKYTQSNGRR- 460
D +V+ +G ADA L+ R Q + E ++ + + L G+R
Sbjct: 52 DDLVIGASGFEADALALVKRIQQRIDLYHDNHERKMSAQSCACMVRTLLY------GKRF 105
Query: 461 -PFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLE 604
P+ + + G D +G ++ +P G Y A G +A + FL+
Sbjct: 106 FPYYVYTTVAGIDKEGKGEIYSFDPVGSYEREWCRAGGSAANFITPFLD 154
>SPAC4A8.13c |pts1||20S proteasome component beta
5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 30.7 bits (66), Expect = 0.19
Identities = 14/72 (19%), Positives = 37/72 (51%)
Frame = +2
Query: 224 KKSVAKLQEERTVRKICLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEY 403
+ S L +TV+K+ ++ +++ AG AD + +EC+ H+L ++ +++
Sbjct: 80 RASAGPLIASQTVKKVIEINPYLLGTLAGGAADCQFWETVLGMECRLHQLRNKELISVSA 139
Query: 404 ITRYIAGLKQKY 439
++ ++ + Y
Sbjct: 140 ASKILSNITYSY 151
>SPBC776.06c |||spindle pole body interacting protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 598
Score = 27.1 bits (57), Expect = 2.3
Identities = 17/41 (41%), Positives = 24/41 (58%), Gaps = 3/41 (7%)
Frame = -3
Query: 339 FINIRASA-VNPANAITT*S--SKRHIFLTVRSSCNLATDF 226
F+N SA +NP N + T SK+ +FLT SS ++ DF
Sbjct: 261 FMNTDISADINPINVLITALLISKKVLFLTKTSSASVLADF 301
>SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 25.8 bits (54), Expect = 5.3
Identities = 27/87 (31%), Positives = 35/87 (40%), Gaps = 10/87 (11%)
Frame = -3
Query: 504 PS*SNPPIKHEIPNGRRPLL*VYFCLRP--------AMYLVIYS-NVTGSSTVNLCD*HS 352
P+ P +IPN R +L + C P MYL S N T S + L HS
Sbjct: 375 PNFPEPKFFEDIPNTERCILSAFICSPPQLPLPNPLRMYLPCPSLNSTEVSVITLAPQHS 434
Query: 351 ICARFINIR-ASAVNPANAITT*SSKR 274
IN+ A A+ IT + KR
Sbjct: 435 FLNIVINLNPALALKSYTIITLLNGKR 461
>SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit
Par2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 627
Score = 25.0 bits (52), Expect = 9.2
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = -2
Query: 502 IIVESSYQTRNTKWSTTIALSIFLFETCYVSCNILQCYWILD 377
II S Y+T N W++TI ++CN+LQ + +D
Sbjct: 549 IIYPSLYKTANEHWNSTIQA---------IACNVLQIFVDMD 581
>SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit
Pst2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1075
Score = 25.0 bits (52), Expect = 9.2
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = -2
Query: 439 IFLFETCYVSCNILQC 392
I LF+TCY+ C + C
Sbjct: 567 IVLFDTCYMVCTYIVC 582
>SPBC6B1.04 |mde4||monopolin-like complex subunit
Mde4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 421
Score = 25.0 bits (52), Expect = 9.2
Identities = 14/38 (36%), Positives = 18/38 (47%)
Frame = +2
Query: 506 SPHLFQTEPSGIYYEWKANATGRSAKTVREFLEKNYTA 619
SP LF I + NAT S +V FL+K T+
Sbjct: 247 SPKLFSPSKQAIMGTRQPNATSGSPLSVTPFLQKTSTS 284
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,586,007
Number of Sequences: 5004
Number of extensions: 53882
Number of successful extensions: 151
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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