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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0054
         (308 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha...    40   8e-05
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|...    37   8e-04
SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S...    36   0.002
SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|...    31   0.052
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1...    28   0.37 
SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochr...    27   0.64 
SPCC757.04 |||transcription factor |Schizosaccharomyces pombe|ch...    25   2.6  
SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pomb...    25   2.6  
SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr...    25   3.4  
SPBC83.18c |||C2 domain protein|Schizosaccharomyces pombe|chr 2|...    25   3.4  
SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster ...    25   3.4  
SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pomb...    24   5.9  
SPBC1A4.01 |apc10|SPBC1E8.06|anaphase-promoting complex subunit ...    24   5.9  
SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ...    24   5.9  

>SPAC16C9.06c |upf1||ATP-dependent RNA helicase
           Upf1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 925

 Score = 39.9 bits (89), Expect = 8e-05
 Identities = 26/79 (32%), Positives = 42/79 (53%)
 Frame = +1

Query: 67  LVKEAKIIAMTCTHAALKRSELLQMGFKYDNILMEESPQILEIETFIPLLLQNPQDGRSR 246
           L++ A +I  TC  A  +R       +K+ ++L++E+ Q  E E  IPL+L         
Sbjct: 534 LLRAAHVICCTCVGAGDRRISK----YKFRSVLIDEATQASEPECMIPLVLG-------- 581

Query: 247 LKRWIMIGDHHQLPPVDKN 303
            K+ +++GDH QL PV  N
Sbjct: 582 AKQVVLVGDHQQLGPVVMN 600


>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
            Sen1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1687

 Score = 36.7 bits (81), Expect = 8e-04
 Identities = 22/97 (22%), Positives = 52/97 (53%)
 Frame = +1

Query: 1    RNWKSSEAFELLRSGLDRSKYLLVKEAKIIAMTCTHAALKRSELLQMGFKYDNILMEESP 180
            +++  ++  +LLR    +++  ++K+A ++  T + +      +      +  ++++E+ 
Sbjct: 1327 QSFTKNKEVDLLRK---KAQKAILKQADVVCATLSGSG--HDLVAHSSLNFSTVIIDEAA 1381

Query: 181  QILEIETFIPLLLQNPQDGRSRLKRWIMIGDHHQLPP 291
            Q +E++T IPL        R   K+ I++GD +QLPP
Sbjct: 1382 QAVELDTIIPL--------RYGAKKCILVGDPNQLPP 1410


>SPBC29A10.10c |||tRNA-splicing endonuclease positive effector
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1944

 Score = 35.5 bits (78), Expect = 0.002
 Identities = 22/75 (29%), Positives = 40/75 (53%)
 Frame = +1

Query: 67   LVKEAKIIAMTCTHAALKRSELLQMGFKYDNILMEESPQILEIETFIPLLLQNPQDGRSR 246
            L++EA I+  T +  A     LL  G  +  ++++E+ Q +E+ + IPL        +  
Sbjct: 1484 LLQEADIVCATLS--ASGHELLLNAGLTFRTVIIDEAAQAVELSSIIPL--------KYG 1533

Query: 247  LKRWIMIGDHHQLPP 291
             +  +M+GD +QLPP
Sbjct: 1534 CESCVMVGDPNQLPP 1548


>SPBC16D10.04c |dna2||DNA replication endonuclease-helicase
            Dna2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1398

 Score = 30.7 bits (66), Expect = 0.052
 Identities = 9/18 (50%), Positives = 17/18 (94%)
 Frame = +1

Query: 250  KRWIMIGDHHQLPPVDKN 303
            ++++++GDH+QLPP+ KN
Sbjct: 1084 EKFVLVGDHYQLPPLVKN 1101


>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 574

 Score = 27.9 bits (59), Expect = 0.37
 Identities = 19/55 (34%), Positives = 24/55 (43%), Gaps = 7/55 (12%)
 Frame = +3

Query: 153 RQHTDGGITSNPRDRDVHTAAPAEPA---GRQVP----AEALDHDRRPPPAAPGR 296
           R +  G I   P+ R      P   A   GRQ P    + A+ +   PPPA PGR
Sbjct: 344 RSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGR 398



 Score = 25.0 bits (52), Expect = 2.6
 Identities = 13/33 (39%), Positives = 16/33 (48%), Gaps = 2/33 (6%)
 Frame = +3

Query: 216 PAEPAGRQVPAEAL--DHDRRPPPAAPGRQEHG 308
           P  P   +V A AL  +  R PPP  P R+  G
Sbjct: 291 PLPPPSSRVSAAALAANKKRPPPPPPPSRRNRG 323


>SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated
           heterochromatin assembly Hrr1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1015

 Score = 27.1 bits (57), Expect = 0.64
 Identities = 23/76 (30%), Positives = 38/76 (50%)
 Frame = +1

Query: 64  LLVKEAKIIAMTCTHAALKRSELLQMGFKYDNILMEESPQILEIETFIPLLLQNPQDGRS 243
           +L++ A +I MT T     R  L ++  K     +EE+  +LE    IP +  +      
Sbjct: 678 ILLRGANVIGMTTTGLNKYRDILERINPKI--CFIEEAADVLE-GPIIPAVFPS------ 728

Query: 244 RLKRWIMIGDHHQLPP 291
            L++ ++IGDH QL P
Sbjct: 729 -LEQLVLIGDHKQLRP 743


>SPCC757.04 |||transcription factor |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 684

 Score = 25.0 bits (52), Expect = 2.6
 Identities = 9/32 (28%), Positives = 18/32 (56%)
 Frame = +1

Query: 181 QILEIETFIPLLLQNPQDGRSRLKRWIMIGDH 276
           Q+  +E+FI  L  +  +GR  + + ++  DH
Sbjct: 107 QLARLESFIMTLKNSDPEGRDEMLKSVVFSDH 138


>SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1647

 Score = 25.0 bits (52), Expect = 2.6
 Identities = 9/24 (37%), Positives = 13/24 (54%)
 Frame = -1

Query: 155 SYLKPICNNSERLRAACVHVIAII 84
           SYL+ +C N   L   C+  I I+
Sbjct: 156 SYLQSLCENGNNLVKTCIDTIPIL 179


>SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1516

 Score = 24.6 bits (51), Expect = 3.4
 Identities = 9/26 (34%), Positives = 19/26 (73%)
 Frame = +3

Query: 108 RRPQTFRIIANGFQIRQHTDGGITSN 185
           +R ++F+ +A+GF  R+HT+  + S+
Sbjct: 794 KRVRSFQAVAHGFLSRRHTEYELLSS 819


>SPBC83.18c |||C2 domain protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 272

 Score = 24.6 bits (51), Expect = 3.4
 Identities = 10/33 (30%), Positives = 15/33 (45%)
 Frame = +3

Query: 207 TAAPAEPAGRQVPAEALDHDRRPPPAAPGRQEH 305
           T  P +P+    P + +      PP  P R+EH
Sbjct: 153 TPVPKKPSKPSKPRKKVPVSHPLPPTPPSREEH 185


>SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster
           type |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 827

 Score = 24.6 bits (51), Expect = 3.4
 Identities = 13/34 (38%), Positives = 19/34 (55%)
 Frame = +1

Query: 106 HAALKRSELLQMGFKYDNILMEESPQILEIETFI 207
           HA  K  E L++  KY   L EES   L++ +F+
Sbjct: 53  HAPEKYIEFLELRLKYLRGLAEESDPNLKLPSFL 86


>SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1073

 Score = 23.8 bits (49), Expect = 5.9
 Identities = 10/22 (45%), Positives = 13/22 (59%)
 Frame = +1

Query: 193 IETFIPLLLQNPQDGRSRLKRW 258
           I T +PL LQ+P D  S +  W
Sbjct: 657 ISTDLPLTLQSPSDEFSTIYAW 678


>SPBC1A4.01 |apc10|SPBC1E8.06|anaphase-promoting complex subunit
           Apc10|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 189

 Score = 23.8 bits (49), Expect = 5.9
 Identities = 12/23 (52%), Positives = 12/23 (52%)
 Frame = +3

Query: 36  EVRTGSVQVPVGQRSENNRDDVH 104
           E  TG V VPVG    N   DVH
Sbjct: 115 EEPTGWVHVPVGDFGRNGLLDVH 137


>SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor
           Ste6|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 911

 Score = 23.8 bits (49), Expect = 5.9
 Identities = 10/29 (34%), Positives = 18/29 (62%)
 Frame = -1

Query: 89  IIFASLTNRYLDRSSPDLSSSKASELFQF 3
           I+F SL  ++L    P+L+ ++ S+L  F
Sbjct: 417 IVFLSLEYKFLTNIQPELNDAEKSQLDGF 445


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,226,328
Number of Sequences: 5004
Number of extensions: 21958
Number of successful extensions: 73
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 70
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 73
length of database: 2,362,478
effective HSP length: 63
effective length of database: 2,047,226
effective search space used: 79841814
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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