Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0054
(308 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g38770.1 68415.m04760 expressed protein 155 4e-39
At2g03270.1 68415.m00280 DNA-binding protein, putative similar t... 33 0.039
At1g16800.1 68414.m02018 tRNA-splicing endonuclease positive eff... 33 0.039
At1g08840.1 68414.m00984 DNA replication helicase, putative simi... 30 0.28
At1g65810.1 68414.m07468 tRNA-splicing endonuclease positive eff... 30 0.36
At3g43300.1 68416.m04570 guanine nucleotide exchange family prot... 28 1.1
At3g13225.1 68416.m01660 WW domain-containing protein contains P... 28 1.5
At1g65780.1 68414.m07465 tRNA-splicing endonuclease positive eff... 28 1.5
At1g66260.1 68414.m07522 RNA and export factor-binding protein, ... 27 1.9
At1g50040.1 68414.m05615 expressed protein 27 2.6
At1g34355.1 68414.m04265 forkhead-associated domain-containing p... 27 2.6
At1g51450.1 68414.m05791 SPla/RYanodine receptor (SPRY) domain-c... 27 3.4
At1g31870.1 68414.m03917 expressed protein 26 4.5
At2g46240.1 68415.m05750 IQ domain-containing protein / BAG doma... 26 5.9
At1g64570.1 68414.m07319 expressed protein 26 5.9
At5g37720.1 68418.m04541 RNA and export factor-binding protein, ... 25 7.8
At2g44550.1 68415.m05543 glycosyl hydrolase family 9 protein 25 7.8
At2g26890.1 68415.m03226 DNAJ heat shock N-terminal domain-conta... 25 7.8
>At2g38770.1 68415.m04760 expressed protein
Length = 1509
Score = 155 bits (377), Expect = 4e-39
Identities = 71/95 (74%), Positives = 83/95 (87%)
Frame = +1
Query: 22 AFELLRSGLDRSKYLLVKEAKIIAMTCTHAALKRSELLQMGFKYDNILMEESPQILEIET 201
AFELL+S DR+ YL+ K+AKI+AMTCTHAALKR + LQ+GFKYDN+LMEES QILEIET
Sbjct: 1074 AFELLKSTADRANYLMTKQAKIVAMTCTHAALKRRDFLQLGFKYDNLLMEESAQILEIET 1133
Query: 202 FIPLLLQNPQDGRSRLKRWIMIGDHHQLPPVDKNM 306
FIP+LLQ +DG +RLKR I+IGDHHQLPPV KNM
Sbjct: 1134 FIPMLLQRQEDGHARLKRCILIGDHHQLPPVVKNM 1168
>At2g03270.1 68415.m00280 DNA-binding protein, putative similar to
Swiss-Prot:Q60560 DNA-binding protein SMUBP-2
(Immunoglobulin MU binding protein 2) (SMUBP-2) (Insulin
II gene enhancer-binding protein)(RIPE3B-binding complex
3B2 P110 subunit) (RIP-1)[Mesocricetus auratus];
identical to putative helicase (atpc-2 gene) cDNA
NCBI_gi:11191230
Length = 639
Score = 33.1 bits (72), Expect = 0.039
Identities = 23/75 (30%), Positives = 38/75 (50%)
Frame = +1
Query: 67 LVKEAKIIAMTCTHAALKRSELLQMGFKYDNILMEESPQILEIETFIPLLLQNPQDGRSR 246
++K A +I T T A ++ + +D ++++E Q LE+ +I LL +
Sbjct: 339 VIKNADVILTTLTGALTRKLD----NRTFDLVIIDEGAQALEVACWIALL---------K 385
Query: 247 LKRWIMIGDHHQLPP 291
R I+ GDH QLPP
Sbjct: 386 GSRCILAGDHLQLPP 400
>At1g16800.1 68414.m02018 tRNA-splicing endonuclease positive
effector-related contains similarity to SEN1, a positive
effector of tRNA-splicing endonuclease [Saccharomyces
cerevisiae] gi|172574|gb|AAB63976
Length = 1939
Score = 33.1 bits (72), Expect = 0.039
Identities = 19/46 (41%), Positives = 28/46 (60%)
Frame = +1
Query: 151 YDNILMEESPQILEIETFIPLLLQNPQDGRSRLKRWIMIGDHHQLP 288
+D ++++E+ Q LE T IPL L +SR + IM+GD QLP
Sbjct: 1429 FDAVVIDEAAQALEPATLIPLQLL-----KSRGTKCIMVGDPKQLP 1469
>At1g08840.1 68414.m00984 DNA replication helicase, putative similar
to helicase [Xenopus laevis] gi|18845092|gb|AAL79550
Length = 1296
Score = 30.3 bits (65), Expect = 0.28
Identities = 20/73 (27%), Positives = 39/73 (53%)
Frame = +1
Query: 76 EAKIIAMTCTHAALKRSELLQMGFKYDNILMEESPQILEIETFIPLLLQNPQDGRSRLKR 255
+ K++A TC L + L + ++D +++E+ QI + PLL +
Sbjct: 993 QVKVVASTC----LGINSPLLVNRRFDVCIIDEAGQIALPVSIGPLLFAST--------- 1039
Query: 256 WIMIGDHHQLPPV 294
++++GDH+QLPP+
Sbjct: 1040 FVLVGDHYQLPPL 1052
>At1g65810.1 68414.m07468 tRNA-splicing endonuclease positive
effector-related contains similarity to SEN1, a positive
effector of tRNA-splicing endonuclease [Saccharomyces
cerevisiae] gi|172574|gb|AAB63976
Length = 1050
Score = 29.9 bits (64), Expect = 0.36
Identities = 20/60 (33%), Positives = 32/60 (53%)
Frame = +1
Query: 124 SELLQMGFKYDNILMEESPQILEIETFIPLLLQNPQDGRSRLKRWIMIGDHHQLPPVDKN 303
S L M ++++E+ Q+ E E+ IPL L+ L+ I+IGD QLP + K+
Sbjct: 544 SARLHMSSPIQLLVIDEAAQLKECESAIPLQLRG-------LQHAILIGDEKQLPAMIKS 596
>At3g43300.1 68416.m04570 guanine nucleotide exchange family protein
similar to SP|Q9Y6D5 Brefeldin A-inhibited guanine
nucleotide-exchange protein 2 {Homo sapiens}; contains
Pfam profile PF01369: Sec7 domain
Length = 1756
Score = 28.3 bits (60), Expect = 1.1
Identities = 20/70 (28%), Positives = 32/70 (45%), Gaps = 2/70 (2%)
Frame = +3
Query: 75 RSENNRDDVHARRPQTF--RIIANGFQIRQHTDGGITSNPRDRDVHTAAPAEPAGRQVPA 248
R++ + DDV+ +R QTF R + N F +R +TS P+ P+ P + P
Sbjct: 1486 RAQKDVDDVNLQRSQTFGQRFMDNLF-LR-----NLTSQPKSSVAEVTVPSSPYKHEDPT 1539
Query: 249 EALDHDRRPP 278
E + P
Sbjct: 1540 EPDSREEESP 1549
>At3g13225.1 68416.m01660 WW domain-containing protein contains Pfam
profile PF00397: WW domain
Length = 863
Score = 27.9 bits (59), Expect = 1.5
Identities = 12/45 (26%), Positives = 20/45 (44%)
Frame = +3
Query: 147 QIRQHTDGGITSNPRDRDVHTAAPAEPAGRQVPAEALDHDRRPPP 281
Q+ +DG + S ++HT P P +P + + PPP
Sbjct: 485 QVIDASDGKMFSQTEPSNLHTDVPPPPGEEWIPPPPSESEDVPPP 529
>At1g65780.1 68414.m07465 tRNA-splicing endonuclease positive
effector-related contains similarity to SEN1, a positive
effector of tRNA-splicing endonuclease [Saccharomyces
cerevisiae] gi|172574|gb|AAB63976
Length = 1065
Score = 27.9 bits (59), Expect = 1.5
Identities = 20/74 (27%), Positives = 34/74 (45%)
Frame = +1
Query: 67 LVKEAKIIAMTCTHAALKRSELLQMGFKYDNILMEESPQILEIETFIPLLLQNPQDGRSR 246
L+KE + + S L G ++++E+ Q+ E E+ IP+ L
Sbjct: 536 LIKELCLGHACLLFSTASCSARLYTGTPIQLLVIDEAAQLKECESSIPMQLPG------- 588
Query: 247 LKRWIMIGDHHQLP 288
L+ I++GD QLP
Sbjct: 589 LRHLILVGDERQLP 602
>At1g66260.1 68414.m07522 RNA and export factor-binding protein,
putative similar to GI:7159943 from [Mus musculus] (RNA
6 (4), 638-650 (2000))
Length = 295
Score = 27.5 bits (58), Expect = 1.9
Identities = 11/27 (40%), Positives = 19/27 (70%)
Frame = +1
Query: 115 LKRSELLQMGFKYDNILMEESPQILEI 195
++RS+ +Q KY+N+L++ P LEI
Sbjct: 155 MRRSDAIQAMRKYNNVLLDGRPMKLEI 181
>At1g50040.1 68414.m05615 expressed protein
Length = 460
Score = 27.1 bits (57), Expect = 2.6
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -1
Query: 275 WSPIMIQRFSRDLPSCGFCRSSG 207
WS + ++ +SR SCGF +SG
Sbjct: 102 WSVLSVEVYSRRSASCGFVAASG 124
>At1g34355.1 68414.m04265 forkhead-associated domain-containing
protein / FHA domain-containing protein
Length = 1477
Score = 27.1 bits (57), Expect = 2.6
Identities = 14/44 (31%), Positives = 20/44 (45%)
Frame = +3
Query: 129 IIANGFQIRQHTDGGITSNPRDRDVHTAAPAEPAGRQVPAEALD 260
I A ++ QH T+ D D+H +E G VP+E D
Sbjct: 328 IEAENLEVAQHQSLANTALGDDEDLHLDVTSEGTGSSVPSEDED 371
>At1g51450.1 68414.m05791 SPla/RYanodine receptor (SPRY)
domain-containing protein low similarity to DEAD box
protein DDX1 [Gallus gallus] GI:16323037, ryanodine
receptor [Caenorhabditis elegans] GI:1871447; contains
Pfam profile PF00622: SPRY domain
Length = 509
Score = 26.6 bits (56), Expect = 3.4
Identities = 14/42 (33%), Positives = 23/42 (54%), Gaps = 2/42 (4%)
Frame = +3
Query: 183 NPR--DRDVHTAAPAEPAGRQVPAEALDHDRRPPPAAPGRQE 302
NP+ + V + P EP G +P+E +D D PP ++ +E
Sbjct: 16 NPKIEEAQVSVSLPEEPTGVLLPSELVD-DSAPPESSDAVEE 56
>At1g31870.1 68414.m03917 expressed protein
Length = 561
Score = 26.2 bits (55), Expect = 4.5
Identities = 13/42 (30%), Positives = 20/42 (47%)
Frame = +3
Query: 153 RQHTDGGITSNPRDRDVHTAAPAEPAGRQVPAEALDHDRRPP 278
R+ T I+ R R + + EP R+ A+ +D D PP
Sbjct: 109 REDTQSNISPPRRQRTRNDSPSPEPGPRRSVADRVDTDMSPP 150
>At2g46240.1 68415.m05750 IQ domain-containing protein / BAG
domain-containing protein contains Pfam profiles
PF00612: IQ calmodulin-binding motif, PF02179: BAG
(Apoptosis regulator Bcl-2 protein) domain
Length = 1043
Score = 25.8 bits (54), Expect = 5.9
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = -3
Query: 294 DRGQLVVVADHDPTLQPGPAVLRV 223
DRG VV+ +H+P ++ G AVL V
Sbjct: 122 DRG--VVIEEHEPEIEKGEAVLPV 143
>At1g64570.1 68414.m07319 expressed protein
Length = 1239
Score = 25.8 bits (54), Expect = 5.9
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = +3
Query: 9 EEFRGLRTTEVRTGSVQVPVGQRSENNRDD 98
EE+R + +G +VP+ Q N+ DD
Sbjct: 179 EEYRKFLAAVLHSGDTEVPLAQTGTNDDDD 208
>At5g37720.1 68418.m04541 RNA and export factor-binding protein,
putative transcriptional coactivator ALY, Mus musculus,
EMBL:MMU89876
Length = 288
Score = 25.4 bits (53), Expect = 7.8
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 118 KRSELLQMGFKYDNILMEESPQILEI 195
+RS+ Q KY+N+L++ P LEI
Sbjct: 142 RRSDAFQALKKYNNVLLDGRPMRLEI 167
>At2g44550.1 68415.m05543 glycosyl hydrolase family 9 protein
Length = 490
Score = 25.4 bits (53), Expect = 7.8
Identities = 15/43 (34%), Positives = 24/43 (55%)
Frame = +1
Query: 55 SKYLLVKEAKIIAMTCTHAALKRSELLQMGFKYDNILMEESPQ 183
SKYL EA ++ C AL+ S+LLQ+ + ++ +PQ
Sbjct: 356 SKYL---EAAKASIDCPDGALQASDLLQVARSQVDYILGSNPQ 395
>At2g26890.1 68415.m03226 DNAJ heat shock N-terminal domain-containing
protein contains Pfam profile PF00226: DnaJ domain
Length = 2554
Score = 25.4 bits (53), Expect = 7.8
Identities = 12/32 (37%), Positives = 22/32 (68%)
Frame = +1
Query: 49 DRSKYLLVKEAKIIAMTCTHAALKRSELLQMG 144
DRS LLV ++++++TC ++L EL++ G
Sbjct: 1637 DRSP-LLVAASELVSLTCAASSLNGEELVRDG 1667
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,725,102
Number of Sequences: 28952
Number of extensions: 125670
Number of successful extensions: 393
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 387
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 393
length of database: 12,070,560
effective HSP length: 70
effective length of database: 10,043,920
effective search space used: 321405440
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -