BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0048 (702 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g17280.1 68414.m02105 ubiquitin-conjugating enzyme, putative ... 29 2.3 At5g44000.1 68418.m05384 glutathione S-transferase C-terminal do... 27 9.1 >At1g17280.1 68414.m02105 ubiquitin-conjugating enzyme, putative similar to ubiquitin conjugating enzyme 6 from [Homo sapiens] GI:14029267, [Mus musculus] GI:14029263; contains Pfam profile PF00179: Ubiquitin-conjugating enzyme Length = 237 Score = 29.5 bits (63), Expect = 2.3 Identities = 17/70 (24%), Positives = 33/70 (47%) Frame = +1 Query: 76 KAFPVSPGQVGEQRLSQEGWDLLTTARAPPKET*QLKSSCFANKSTTGSKSRPTEKIRRE 255 K FP + +Q+L+++ LTT +P K +++S + + S+ E+ + Sbjct: 151 KLFPEYVEKYNQQQLAEQATTQLTTPESPQKSDTKVESEKTIDPTKGDSEGGLKERKKNN 210 Query: 256 TQRADAWVRL 285 Q AW+ L Sbjct: 211 KQGLPAWIIL 220 >At5g44000.1 68418.m05384 glutathione S-transferase C-terminal domain-containing protein contains Pfam domain PF00043: Glutathione S-transferase, C-terminal domain Length = 399 Score = 27.5 bits (58), Expect = 9.1 Identities = 19/57 (33%), Positives = 29/57 (50%), Gaps = 4/57 (7%) Frame = +1 Query: 139 LLTTARAPPKET*QLKSSCFANKSTTGSKSRPTEKIRRE----TQRADAWVRLHVDL 297 L++TAR QL + A ++GS +RPT K R + T A + + L+V L Sbjct: 56 LISTARTAWTTVWQLMMTQLAPSDSSGSYTRPTSKFRLDPTQFTSAASSELHLYVGL 112 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,469,773 Number of Sequences: 28952 Number of extensions: 217609 Number of successful extensions: 445 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 442 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 445 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1506636208 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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