BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0048
(702 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g17280.1 68414.m02105 ubiquitin-conjugating enzyme, putative ... 29 2.3
At5g44000.1 68418.m05384 glutathione S-transferase C-terminal do... 27 9.1
>At1g17280.1 68414.m02105 ubiquitin-conjugating enzyme, putative
similar to ubiquitin conjugating enzyme 6 from [Homo
sapiens] GI:14029267, [Mus musculus] GI:14029263;
contains Pfam profile PF00179: Ubiquitin-conjugating
enzyme
Length = 237
Score = 29.5 bits (63), Expect = 2.3
Identities = 17/70 (24%), Positives = 33/70 (47%)
Frame = +1
Query: 76 KAFPVSPGQVGEQRLSQEGWDLLTTARAPPKET*QLKSSCFANKSTTGSKSRPTEKIRRE 255
K FP + +Q+L+++ LTT +P K +++S + + S+ E+ +
Sbjct: 151 KLFPEYVEKYNQQQLAEQATTQLTTPESPQKSDTKVESEKTIDPTKGDSEGGLKERKKNN 210
Query: 256 TQRADAWVRL 285
Q AW+ L
Sbjct: 211 KQGLPAWIIL 220
>At5g44000.1 68418.m05384 glutathione S-transferase C-terminal
domain-containing protein contains Pfam domain PF00043:
Glutathione S-transferase, C-terminal domain
Length = 399
Score = 27.5 bits (58), Expect = 9.1
Identities = 19/57 (33%), Positives = 29/57 (50%), Gaps = 4/57 (7%)
Frame = +1
Query: 139 LLTTARAPPKET*QLKSSCFANKSTTGSKSRPTEKIRRE----TQRADAWVRLHVDL 297
L++TAR QL + A ++GS +RPT K R + T A + + L+V L
Sbjct: 56 LISTARTAWTTVWQLMMTQLAPSDSSGSYTRPTSKFRLDPTQFTSAASSELHLYVGL 112
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,469,773
Number of Sequences: 28952
Number of extensions: 217609
Number of successful extensions: 445
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 442
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 445
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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