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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0046
         (505 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g23290.1 68414.m02913 60S ribosomal protein L27A (RPL27aB) si...   120   4e-28
At1g70600.1 68414.m08133 60S ribosomal protein L27A (RPL27aC) id...   117   4e-27
At1g12960.1 68414.m01505 60S ribosomal protein L27A (RPL27aA) si...    46   1e-05
At2g16100.1 68415.m01846 hypothetical protein                          29   2.4  
At4g29090.1 68417.m04163 reverse transcriptase, putative / RNA-d...    27   5.4  

>At1g23290.1 68414.m02913 60S ribosomal protein L27A (RPL27aB)
           similar to 60S RIBOSOMAL PROTEIN L27A GB:P49637
           GI:1710530 from [Arabidopsis thaliana]
          Length = 146

 Score =  120 bits (290), Expect = 4e-28
 Identities = 65/149 (43%), Positives = 82/149 (55%), Gaps = 1/149 (0%)
 Frame = +2

Query: 2   MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 181
           MAT+ KK RK RGHVS                 NAGG HHHRI  DKYHPGYFGK+GMR 
Sbjct: 1   MATALKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60

Query: 182 FHFRKNKNFCPVLNLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK- 358
           FH  +NK FCP++NLDKLW+LV E  + K  S+ D  VP+I++ +            P+ 
Sbjct: 61  FHKLRNKFFCPIVNLDKLWSLVPEDVKAK--SSKD-NVPLIDVTQHGFFKVLGKGHLPEN 117

Query: 359 QPVIVXXXXXXXXXXXXXXDVGGACVLSA 445
           +P +V              + GGA VL+A
Sbjct: 118 KPFVVKAKLISKTAEKKIKEAGGAVVLTA 146


>At1g70600.1 68414.m08133 60S ribosomal protein L27A (RPL27aC)
           identical to 60S ribosomal protein L27A GB:P49637
           [Arabidopsis thaliana]
          Length = 146

 Score =  117 bits (282), Expect = 4e-27
 Identities = 64/149 (42%), Positives = 79/149 (53%), Gaps = 1/149 (0%)
 Frame = +2

Query: 2   MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 181
           M T  KK RK RGHVS                 NAGG HHHRI  DKYHPGYFGK+GMR 
Sbjct: 1   MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60

Query: 182 FHFRKNKNFCPVLNLDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK- 358
           FH  +NK FCP++NLDKLW+LV E  + K  S  D  VP+I++ +            P+ 
Sbjct: 61  FHKLRNKFFCPIVNLDKLWSLVPEDVKAK--STKD-NVPLIDVTQHGFFKVLGKGHLPEN 117

Query: 359 QPVIVXXXXXXXXXXXXXXDVGGACVLSA 445
           +P +V              + GGA VL+A
Sbjct: 118 KPFVVKAKLISKTAEKKIKEAGGAVVLTA 146


>At1g12960.1 68414.m01505 60S ribosomal protein L27A (RPL27aA)
           similar to GB:BAA96068 from [Panax ginseng]
          Length = 104

 Score = 46.4 bits (105), Expect = 1e-05
 Identities = 32/84 (38%), Positives = 41/84 (48%), Gaps = 2/84 (2%)
 Frame = +2

Query: 2   MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGK--LGM 175
           M TS+KKTR LR HVS                       H R    +  PG  G   +GM
Sbjct: 1   MTTSRKKTRNLREHVSVG---------------------HGRFGKHRKLPGSRGNAGVGM 39

Query: 176 RNFHFRKNKNFCPVLNLDKLWTLV 247
           R FH  +NK +C ++NLDKLW++V
Sbjct: 40  RYFHKLRNKFYCQIVNLDKLWSMV 63


>At2g16100.1 68415.m01846 hypothetical protein 
          Length = 250

 Score = 28.7 bits (61), Expect = 2.4
 Identities = 9/19 (47%), Positives = 13/19 (68%)
 Frame = +1

Query: 115 APSQNQHGQVPSWILWQTW 171
           +P  +Q  Q+P WILW+ W
Sbjct: 20  SPRTSQFHQLPLWILWRIW 38


>At4g29090.1 68417.m04163 reverse transcriptase, putative /
           RNA-dependent DNA polymerase, putative similar to
           reverse transcriptase [Arabidopsis thaliana] GI:976278;
           contains Pfam profile PF00075: RNase H
          Length = 575

 Score = 27.5 bits (58), Expect = 5.4
 Identities = 23/77 (29%), Positives = 34/77 (44%), Gaps = 13/77 (16%)
 Frame = +1

Query: 118 PSQNQHGQVPSWILWQTWYEKFPL-*KEQEFLSSFELR*A---LDISL*TDEAEVC---- 273
           P   +  Q+  W+LW+ W  +  L  + +EF +   LR A   L+      EAE C    
Sbjct: 351 PQWEKASQLVPWLLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWRIRTEAESCGTKP 410

Query: 274 -----ICSRWQGPRHQY 309
                 C RW+ P HQ+
Sbjct: 411 QVNRSSCGRWRPPPHQW 427


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,508,383
Number of Sequences: 28952
Number of extensions: 190430
Number of successful extensions: 486
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 466
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 484
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 898188928
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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