BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0033 (671 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 33 0.050 SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyc... 28 1.1 SPBC16G5.09 |||serine carboxypeptidase |Schizosaccharomyces pomb... 26 4.3 SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr ... 26 5.7 SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces... 26 5.7 SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor c... 25 7.5 SPBC337.15c |coq7||ubiquinone biosynthesis protein Coq7|Schizosa... 25 7.5 SPBC725.08 |||transcription factor |Schizosaccharomyces pombe|ch... 25 9.9 SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 9.9 >SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 32.7 bits (71), Expect = 0.050 Identities = 22/64 (34%), Positives = 30/64 (46%), Gaps = 3/64 (4%) Frame = -1 Query: 440 QNSPN*HPYALHRR-YQSSLSYPGPRVSSTY-RKDPPSFHRCWRLTSPSCSFYH-SHHYA 270 Q PN P+ +H Y S SYP P +TY +PP + S S H S +Y+ Sbjct: 193 QVHPNRLPHPIHNHPYSSPTSYPPPLCPATYCPSNPPQLAPATAIAPSSQSSQHKSVNYS 252 Query: 269 TSPS 258 +PS Sbjct: 253 VTPS 256 >SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 28.3 bits (60), Expect = 1.1 Identities = 14/34 (41%), Positives = 18/34 (52%) Frame = -1 Query: 491 NRLLR*VGYNKNSS*IDQNSPN*HPYALHRRYQS 390 N L R N S +DQN HPY L +RY++ Sbjct: 208 NNLTRTFWANDEPSPVDQNMYGSHPYYLEQRYKA 241 >SPBC16G5.09 |||serine carboxypeptidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 510 Score = 26.2 bits (55), Expect = 4.3 Identities = 12/23 (52%), Positives = 13/23 (56%) Frame = -2 Query: 133 EVHLGTVLVDSWGECLLWFFPLG 65 E LG L SW ECLL F +G Sbjct: 228 ESELGQYLHHSWAECLLAFDKIG 250 >SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr 1|||Manual Length = 1854 Score = 25.8 bits (54), Expect = 5.7 Identities = 17/49 (34%), Positives = 22/49 (44%) Frame = +1 Query: 46 DDLRQVNQEERTKEDTHPSYRPKRFPSELLLRSSHTLRGINSALASFNI 192 DD V + D P P E+LLR SH+L N+ SF+I Sbjct: 1771 DDNHSVEDHLKVPTDNEPRRSPSL--KEVLLRGSHSLHS-NNDRTSFDI 1816 >SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 995 Score = 25.8 bits (54), Expect = 5.7 Identities = 13/35 (37%), Positives = 18/35 (51%) Frame = -1 Query: 491 NRLLR*VGYNKNSS*IDQNSPN*HPYALHRRYQSS 387 N L R N N + +D+N+ HP+ L RY S Sbjct: 224 NNLTRTFWANGNPTPLDRNAYGTHPFYLEHRYTPS 258 >SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor complex subunit Rrn5|Schizosaccharomyces pombe|chr 1|||Manual Length = 556 Score = 25.4 bits (53), Expect = 7.5 Identities = 12/25 (48%), Positives = 14/25 (56%) Frame = +2 Query: 275 NGVNGKMNSLARSNASIYGTKEDLY 349 NG+N S S ASI G+ E LY Sbjct: 6 NGLNESEGSTPLSTASIIGSSEQLY 30 >SPBC337.15c |coq7||ubiquinone biosynthesis protein Coq7|Schizosaccharomyces pombe|chr 2|||Manual Length = 216 Score = 25.4 bits (53), Expect = 7.5 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = -3 Query: 117 PFWSIAGVSVFFGSFLLVDLAQIIHTSRTKNII 19 PFW IAG ++ G+ LL A + T + +I Sbjct: 109 PFWDIAGFALGAGTALLGTKAAMACTEAVETVI 141 >SPBC725.08 |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 609 Score = 25.0 bits (52), Expect = 9.9 Identities = 9/36 (25%), Positives = 16/36 (44%) Frame = +2 Query: 317 ASIYGTKEDLYDRWKRHADPGKKEKTDSDGEGHRGA 424 + ++ E Y RWKR P + ++ G + A Sbjct: 2 SEVHQESEVEYSRWKRERSPERSQRRSQSPPGEQSA 37 >SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 547 Score = 25.0 bits (52), Expect = 9.9 Identities = 13/56 (23%), Positives = 26/56 (46%) Frame = +2 Query: 155 YAESIAPSHHSTYAHYYDDEEDGWEMPNFYNETYMKERLHNGVNGKMNSLARSNAS 322 Y ++ A H+ AH Y E + + ++ + Y+ L + +GK+ L+ S Sbjct: 285 YHDAHAKQHYVDTAHCYAMELETQRVWDYAGDNYVHRLLQSETDGKLVELSTDGKS 340 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,757,438 Number of Sequences: 5004 Number of extensions: 56342 Number of successful extensions: 168 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 168 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 168 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 307866294 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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