SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= S13A01NGRL0001_B08
         (543 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|ch...    29   0.59 
SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|c...    27   1.4  
SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|ch...    27   2.4  
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces...    26   3.1  
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr...    26   3.1  
SPBC15D4.01c ||SPBC2D10.21c|kinesin-like protein|Schizosaccharom...    26   4.1  
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz...    26   4.1  
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha...    26   4.1  
SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces...    26   4.1  
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos...    25   7.2  
SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha...    25   9.5  
SPBC28F2.08c |||HRD ubiquitin ligase complex subunit |Schizosacc...    25   9.5  
SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces...    25   9.5  
SPBC1604.13c |mrpl32||mitochondrial ribosomal protein subunit L3...    25   9.5  
SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr ...    25   9.5  

>SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 534

 Score = 28.7 bits (61), Expect = 0.59
 Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 3/30 (10%)
 Frame = -2

Query: 281 RGSAVRKSVQCS---PPLWGYVQVYNSQMP 201
           +G ++ KS  CS   PPL G+V V+N Q P
Sbjct: 256 KGKSIIKSQNCSDGLPPLPGFVVVFNLQSP 285


>SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 589

 Score = 27.5 bits (58), Expect = 1.4
 Identities = 14/37 (37%), Positives = 21/37 (56%)
 Frame = -1

Query: 111 CILGFTLSACFISRTRAVKPSLCSDSLSSTGVVPNVF 1
           CI GF ++  + +RTR + PS    +LS T V+   F
Sbjct: 325 CIAGFVVNEFYTTRTRIITPS-AFKTLSLTSVMVTSF 360


>SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 589

 Score = 26.6 bits (56), Expect = 2.4
 Identities = 14/33 (42%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
 Frame = -1

Query: 111 CILGFTLSACFISRTRAVKPSLCSD-SLSSTGV 16
           CI GF ++  + +RTR + PS     SLSS  V
Sbjct: 325 CIAGFVVNELYTTRTRIIAPSAFQTLSLSSVMV 357


>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1485

 Score = 26.2 bits (55), Expect = 3.1
 Identities = 21/68 (30%), Positives = 30/68 (44%), Gaps = 5/68 (7%)
 Frame = +2

Query: 29   DKESEHREG---FTALVRE--MKQALNVKPNMQLVISVLPNVNSSIYFDVPSIINLVDIV 193
            D ES H EG   F   + E  MK+ALN    ++  +S     ++ I FD    I   D V
Sbjct: 985  DYESHHGEGNVHFNVTLTEAGMKEALNESLEVKFKLSRTQATSNMIAFDASGRIKKYDSV 1044

Query: 194  NIQAFDYY 217
                 ++Y
Sbjct: 1045 EDILTEFY 1052


>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 780

 Score = 26.2 bits (55), Expect = 3.1
 Identities = 12/31 (38%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
 Frame = +3

Query: 339 PTNLSL-VSAPLDVRGSSIQTVKFPEFHRYT 428
           PT L    SA  DVR +S   +   +FH+Y+
Sbjct: 51  PTELKARTSATFDVRSASTSPINASDFHKYS 81


>SPBC15D4.01c ||SPBC2D10.21c|kinesin-like
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 633

 Score = 25.8 bits (54), Expect = 4.1
 Identities = 21/54 (38%), Positives = 28/54 (51%), Gaps = 6/54 (11%)
 Frame = +2

Query: 353 LGISTTGRTWKLDSDSEISGVPPI------HADKGGEAGPYTKVQGLLSYPEIC 496
           LG+S  G+T+ L   S+  GV  +      +A KG EA P T V+ L S  E C
Sbjct: 93  LGVSGAGKTYTLFGPSDRPGVAFLALDALFYAIKGREASPQT-VEFLRSQLEKC 145


>SPBC1306.01c ||SPBC409.22c|translation elongation factor
           G|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 770

 Score = 25.8 bits (54), Expect = 4.1
 Identities = 9/21 (42%), Positives = 13/21 (61%)
 Frame = +2

Query: 422 IHADKGGEAGPYTKVQGLLSY 484
           +H  + G AG Y KV+G + Y
Sbjct: 562 LHKKQSGGAGQYAKVEGYIEY 582


>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
           Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 884

 Score = 25.8 bits (54), Expect = 4.1
 Identities = 15/53 (28%), Positives = 25/53 (47%), Gaps = 2/53 (3%)
 Frame = +2

Query: 257 PIYAPQNRDPLQNADAAINYWIQNGAPTHKLVLGISTTGRTWK--LDSDSEIS 409
           P   PQ+ + +QN +  +   +  G P  K V+ +  T        DSDS++S
Sbjct: 777 PESPPQSYESIQNDNELLQELLSMGFPREKAVIALEATNYDVNEVSDSDSKLS 829


>SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1009

 Score = 25.8 bits (54), Expect = 4.1
 Identities = 10/22 (45%), Positives = 12/22 (54%)
 Frame = -3

Query: 193 DNVDQVYDAWDVKVNGAIYVWQ 128
           D VD++YDAW    N     WQ
Sbjct: 54  DYVDEMYDAWKKDPNSVHASWQ 75


>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
           Mms2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 139

 Score = 25.0 bits (52), Expect = 7.2
 Identities = 11/32 (34%), Positives = 16/32 (50%)
 Frame = +2

Query: 152 YFDVPSIINLVDIVNIQAFDYYTPERNPKEAD 247
           Y D P I+  V  +N+   D  T + NP + D
Sbjct: 68  YPDAPPIVTFVSRINLPGVDGETGKVNPHKID 99


>SPAC23E2.03c |ste7||meiotic suppressor protein
           Ste7|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 569

 Score = 24.6 bits (51), Expect = 9.5
 Identities = 14/39 (35%), Positives = 21/39 (53%), Gaps = 3/39 (7%)
 Frame = +3

Query: 324 KMVRLPTNLSLVSAPLDVR---GSSIQTVKFPEFHRYTP 431
           +++ +P NL+    PL      GSS +T + PEF   TP
Sbjct: 144 ELIMVPPNLA--GKPLSANSSFGSSAKTFQLPEFDSVTP 180


>SPBC28F2.08c |||HRD ubiquitin ligase complex subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 713

 Score = 24.6 bits (51), Expect = 9.5
 Identities = 12/40 (30%), Positives = 20/40 (50%)
 Frame = +2

Query: 224 ERNPKEADYTAPIYAPQNRDPLQNADAAINYWIQNGAPTH 343
           +R+P  + Y   +  P N    +NA  A+NY+ +  A  H
Sbjct: 451 DRDPSTS-YLKTVIFPTNEQTHRNARIAMNYYSRAAARNH 489


>SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1155

 Score = 24.6 bits (51), Expect = 9.5
 Identities = 13/29 (44%), Positives = 16/29 (55%)
 Frame = -3

Query: 472 TLNFGIRTSFTTLIGVYRWNSGNFTV*IE 386
           T  F  + SFT+   +YRWN  N  V IE
Sbjct: 140 TFTFSDKPSFTS---IYRWNVTNSNVTIE 165


>SPBC1604.13c |mrpl32||mitochondrial ribosomal protein subunit
           L32|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 103

 Score = 24.6 bits (51), Expect = 9.5
 Identities = 13/40 (32%), Positives = 21/40 (52%)
 Frame = +2

Query: 62  ALVREMKQALNVKPNMQLVISVLPNVNSSIYFDVPSIINL 181
           AL+R    A++ K       S LP+++  I+   PSI N+
Sbjct: 2   ALLRGNSLAISQKMLSVFQASALPHISLRIFISPPSIANI 41


>SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 791

 Score = 24.6 bits (51), Expect = 9.5
 Identities = 15/43 (34%), Positives = 18/43 (41%)
 Frame = +2

Query: 212 YYTPERNPKEADYTAPIYAPQNRDPLQNADAAINYWIQNGAPT 340
           Y TP        Y  P Y P  R+P Q+   A +    NG PT
Sbjct: 590 YPTPTMPYIPVGYPVPGYTPYMRNPSQHTSVAPS---PNGTPT 629


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,381,221
Number of Sequences: 5004
Number of extensions: 50389
Number of successful extensions: 149
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 149
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 223909422
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -