BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S13A01NGRL0001_A04
(544 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosacc... 27 1.8
SPCC4F11.04c |||mannosyltransferase complex subunit |Schizosacch... 27 1.8
SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2 |Schizosacc... 26 3.1
SPAC824.02 |||GPI inositol deacylase|Schizosaccharomyces pombe|c... 26 4.1
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 25 5.5
SPAC14C4.08 |mug5||meiotically upregulated gene Mug5|Schizosacch... 25 7.2
SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyce... 25 7.2
SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyc... 25 9.5
>SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 758
Score = 27.1 bits (57), Expect = 1.8
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = +2
Query: 308 VALEIPMMNLLAPSVFPKMF*QTVKATSI 394
+A +PM N+ + PK F +T+K T +
Sbjct: 546 IAAPLPMANVDTDKIIPKQFLKTIKRTGL 574
>SPCC4F11.04c |||mannosyltransferase complex subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 345
Score = 27.1 bits (57), Expect = 1.8
Identities = 11/27 (40%), Positives = 15/27 (55%)
Frame = +1
Query: 346 LGFSQDVLANRQSHEYFSELIHNLNSN 426
+G S DV+ + H YF +I NL N
Sbjct: 190 VGVSNDVMGSVPHHPYFELIIQNLEKN 216
>SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 849
Score = 26.2 bits (55), Expect = 3.1
Identities = 16/50 (32%), Positives = 24/50 (48%), Gaps = 1/50 (2%)
Frame = +3
Query: 345 PRFFPRCFSKPSKPRVFQRTNT*P*QQRHYV-EDTLRQCCICRHTQQATR 491
PR + RC SKP ++ Q + P ++ V D L + C H +A R
Sbjct: 325 PRVYSRCNSKPVSLKIQQLKSLYPSDKKLIVGRDQLTKACGVTHKLRAFR 374
>SPAC824.02 |||GPI inositol deacylase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 4.1
Identities = 16/60 (26%), Positives = 28/60 (46%), Gaps = 6/60 (10%)
Frame = -2
Query: 261 NNVFIFVL--LHLLY*NFISQIVHRIC*VPIIY----YGSGNFFIIRLMRIPRTPIQKYY 100
NNVF+F+L L+Y ++H + + + Y FF+ + + P P KY+
Sbjct: 1073 NNVFLFLLSFYSLIYGAEKPYLIHNVVGLYFFWLLFLYAKNGFFVQNISKWPIIPRMKYF 1132
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 25.4 bits (53), Expect = 5.5
Identities = 23/79 (29%), Positives = 38/79 (48%), Gaps = 4/79 (5%)
Frame = +1
Query: 310 SVGNTYDELASALGFSQDVLANRQSHEYFSELIHNLNSNDTMSKTL--YANAVFVDIHS- 480
S+ Y E+A ++L + S+ ELI ++ N +MSK L Y + I +
Sbjct: 996 SLEECYQEVAYVADTWLEILFTKGSNLTDDELIELISENRSMSKALSEYGSQKSTSITTA 1055
Query: 481 -KLRDVFQDYLTRIYKGIA 534
+L D D +T+ KG+A
Sbjct: 1056 RRLADFLGDQMTK-DKGLA 1073
>SPAC14C4.08 |mug5||meiotically upregulated gene
Mug5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 179
Score = 25.0 bits (52), Expect = 7.2
Identities = 8/20 (40%), Positives = 13/20 (65%)
Frame = -1
Query: 505 SLETHRVACCVCRQIQHWRK 446
+L+T + CC R I+H+ K
Sbjct: 78 TLQTMLIFCCTLRMIEHYEK 97
>SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 844
Score = 25.0 bits (52), Expect = 7.2
Identities = 11/33 (33%), Positives = 17/33 (51%)
Frame = +1
Query: 337 ASALGFSQDVLANRQSHEYFSELIHNLNSNDTM 435
+S++ F Q +A Q +YF LN N+ M
Sbjct: 376 SSSISFRQVCIAETQLQQYFLRFEKRLNGNNRM 408
>SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 969
Score = 24.6 bits (51), Expect = 9.5
Identities = 12/48 (25%), Positives = 22/48 (45%)
Frame = +1
Query: 397 SELIHNLNSNDTMSKTLYANAVFVDIHSKLRDVFQDYLTRIYKGIAVN 540
+E IH L + +++T +AN + + YL + YK +N
Sbjct: 198 AETIHGLRLGNNLTRTFWANDEPSPVDQNMYGSHPYYLEQRYKADGIN 245
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,065,366
Number of Sequences: 5004
Number of extensions: 39753
Number of successful extensions: 123
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 223909422
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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