Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0024_M08
(330 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein L30|Sc... 145 2e-36
SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein L30|Sc... 140 5e-35
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 30 0.10
SPAC977.14c |||aldo/keto reductase, unknown biological role|Schi... 30 0.10
SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces pomb... 27 0.73
SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce... 26 1.7
SPBC3B9.17 |isa2||iron-sulfur protein Isa2|Schizosaccharomyces p... 23 9.0
SPCC1620.14c |snf22|SPCC830.01c|ATP-dependent DNA helicase Snf22... 23 9.0
SPAC23H3.09c |gly1||threonine aldolase |Schizosaccharomyces pomb... 23 9.0
SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual 23 9.0
>SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein
L30|Schizosaccharomyces pombe|chr 1|||Manual
Length = 117
Score = 145 bits (351), Expect = 2e-36
Identities = 66/95 (69%), Positives = 80/95 (84%)
Frame = +3
Query: 39 TAIIMVAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIARNFPPLR 218
TA + +KK KK+ ++INS+LAL MKSGKY LGYK TLKTLR GKAKL++IA N PPLR
Sbjct: 7 TAPVAAVSKKGKKSGDTINSKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAANAPPLR 66
Query: 219 KSEIEYYALLAKTGVHHYSGNNIELGTACGKYYRV 323
KSE+EYYA+L++ VHHYSGNNI+LGTACGK +RV
Sbjct: 67 KSELEYYAMLSRCSVHHYSGNNIDLGTACGKLFRV 101
>SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein
L30|Schizosaccharomyces pombe|chr 1|||Manual
Length = 109
Score = 140 bits (339), Expect = 5e-35
Identities = 63/90 (70%), Positives = 76/90 (84%)
Frame = +3
Query: 54 VAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIARNFPPLRKSEIE 233
V KK KK+ ++IN++LAL MKSGKY LGYK TLKTLR GKAKL++IA N PPLRKSE+E
Sbjct: 4 VVTKKSKKSGDTINAKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAGNCPPLRKSELE 63
Query: 234 YYALLAKTGVHHYSGNNIELGTACGKYYRV 323
YYA+L+K VHHY+G NI+LGTACGK +RV
Sbjct: 64 YYAMLSKANVHHYAGTNIDLGTACGKLFRV 93
>SPAC1834.01 |sup45||translation release factor
eRF1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 433
Score = 29.9 bits (64), Expect = 0.10
Identities = 14/51 (27%), Positives = 27/51 (52%)
Frame = +3
Query: 66 KQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIARNFPPLR 218
++KK I+ ++L SGKYC G T+ L++G + ++ + +R
Sbjct: 279 QEKKLIQRFFDEISL--DSGKYCFGVVDTMNALQEGAVETLLCFADLDMIR 327
>SPAC977.14c |||aldo/keto reductase, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 351
Score = 29.9 bits (64), Expect = 0.10
Identities = 15/45 (33%), Positives = 27/45 (60%)
Frame = +3
Query: 174 KAKLVIIARNFPPLRKSEIEYYALLAKTGVHHYSGNNIELGTACG 308
++ +VI+++ F P+RK I+ + L+ GVH ++ EL CG
Sbjct: 87 RSSIVILSKCFFPVRKDLIKIFGDLSSRGVHFL--DSPELANQCG 129
>SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 910
Score = 27.1 bits (57), Expect = 0.73
Identities = 14/22 (63%), Positives = 15/22 (68%)
Frame = -2
Query: 203 IPGDDHQLSFPLSECLQSLFVA 138
IP DD L FPL ECL S+ VA
Sbjct: 597 IPDDDPNL-FPLFECLSSVAVA 617
>SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 154
Score = 25.8 bits (54), Expect = 1.7
Identities = 10/26 (38%), Positives = 18/26 (69%)
Frame = +3
Query: 138 GYKQTLKTLRQGKAKLVIIARNFPPL 215
G K+ +K +R+G+ LVI+A + P+
Sbjct: 57 GVKEVVKAVRKGEKGLVILAGDISPM 82
>SPBC3B9.17 |isa2||iron-sulfur protein Isa2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 205
Score = 23.4 bits (48), Expect = 9.0
Identities = 17/47 (36%), Positives = 23/47 (48%)
Frame = +3
Query: 168 QGKAKLVIIARNFPPLRKSEIEYYALLAKTGVHHYSGNNIELGTACG 308
+GKA++V + P + SEIEY L G NN T+CG
Sbjct: 153 RGKARVVADNISLPLISGSEIEYTNEL--IGSSFQLLNNPRAKTSCG 197
>SPCC1620.14c |snf22|SPCC830.01c|ATP-dependent DNA helicase
Snf22|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1680
Score = 23.4 bits (48), Expect = 9.0
Identities = 11/43 (25%), Positives = 21/43 (48%)
Frame = +3
Query: 126 KYCLGYKQTLKTLRQGKAKLVIIARNFPPLRKSEIEYYALLAK 254
+YC+ + L L+ + V +PP RK +YY ++ +
Sbjct: 1520 RYCMEIFERLYNLQSEDGRFVNGLFLYPPNRKLYPDYYIIIKR 1562
>SPAC23H3.09c |gly1||threonine aldolase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 376
Score = 23.4 bits (48), Expect = 9.0
Identities = 8/24 (33%), Positives = 15/24 (62%)
Frame = +3
Query: 72 KKTIESINSRLALVMKSGKYCLGY 143
++T+E +V K G++C+GY
Sbjct: 353 RRTVERQAVETHIVAKPGEFCVGY 376
>SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 1279
Score = 23.4 bits (48), Expect = 9.0
Identities = 13/62 (20%), Positives = 34/62 (54%)
Frame = +3
Query: 69 QKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIARNFPPLRKSEIEYYALL 248
+++ ++S +++ ++ SGK + + +R+G+AKL+ + FP + ++ LL
Sbjct: 522 KREELQSNSNKTVIIEISGK-------SQEAVREGRAKLLALVNQFPESKFYKVTIDPLL 574
Query: 249 AK 254
+
Sbjct: 575 QR 576
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,483,674
Number of Sequences: 5004
Number of extensions: 28856
Number of successful extensions: 70
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 70
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 70
length of database: 2,362,478
effective HSP length: 64
effective length of database: 2,042,222
effective search space used: 91899990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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