Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0024_B20
(367 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 151 2e-38
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 151 2e-38
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 151 2e-38
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 46 2e-06
SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz... 27 0.69
SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces p... 26 1.6
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 26 1.6
SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr 3|||Ma... 26 1.6
SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces pom... 25 2.8
SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4 family|S... 25 4.9
SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein Vps33|S... 25 4.9
SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cu... 24 6.4
SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase Lac... 24 8.5
SPAC15E1.05c |||ethanolamine-phosphate cytidylyltransferase |Sch... 24 8.5
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 151 bits (367), Expect = 2e-38
Identities = 73/117 (62%), Positives = 88/117 (75%)
Frame = +1
Query: 16 ANITTEVKSVEMPRGSTRSLYPVTMLVSTSKTYLVQRELRRGYVAGDSKNNPPRGAADFT 195
A +TTEVKSVEM S + P + K V +++RRG V GDSKN+PP G A FT
Sbjct: 281 AGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSV-KDIRRGNVCGDSKNDPPMGCASFT 339
Query: 196 AQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGD 366
AQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E++PK +KSGD
Sbjct: 340 AQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGD 396
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 151 bits (367), Expect = 2e-38
Identities = 73/117 (62%), Positives = 88/117 (75%)
Frame = +1
Query: 16 ANITTEVKSVEMPRGSTRSLYPVTMLVSTSKTYLVQRELRRGYVAGDSKNNPPRGAADFT 195
A +TTEVKSVEM S + P + K V +++RRG V GDSKN+PP G A FT
Sbjct: 281 AGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSV-KDIRRGNVCGDSKNDPPMGCASFT 339
Query: 196 AQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGD 366
AQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E++PK +KSGD
Sbjct: 340 AQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGD 396
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 151 bits (367), Expect = 2e-38
Identities = 73/117 (62%), Positives = 88/117 (75%)
Frame = +1
Query: 16 ANITTEVKSVEMPRGSTRSLYPVTMLVSTSKTYLVQRELRRGYVAGDSKNNPPRGAADFT 195
A +TTEVKSVEM S + P + K V +++RRG V GDSKN+PP G A FT
Sbjct: 281 AGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSV-KDIRRGNVCGDSKNDPPMGCASFT 339
Query: 196 AQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGD 366
AQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E++PK +KSGD
Sbjct: 340 AQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGD 396
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 45.6 bits (103), Expect = 2e-06
Identities = 23/68 (33%), Positives = 37/68 (54%)
Frame = +1
Query: 127 ELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEK 306
+++ GYV +KN P F AQ+ +L P ++ GY+ V+ HTA FA++ K
Sbjct: 540 DVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHK 598
Query: 307 VDRRTGKS 330
+D+ KS
Sbjct: 599 LDKTNRKS 606
>SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1253
Score = 27.5 bits (58), Expect = 0.69
Identities = 9/25 (36%), Positives = 18/25 (72%)
Frame = +1
Query: 43 VEMPRGSTRSLYPVTMLVSTSKTYL 117
++MPRG+ ++YP M++ +TY+
Sbjct: 659 LQMPRGNLETIYPRIMVLDGVRTYI 683
>SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 505
Score = 26.2 bits (55), Expect = 1.6
Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +3
Query: 174 QGSCRLHSASHCAKSPRSNIKRIHTCIGLPHSPHSLQIC-RNQRESRP 314
+GS ++S + +S + I CIG+ SPH +C R Q +P
Sbjct: 90 KGSTCAFTSSILQQIQKSGERSIPKCIGMYTSPHLRSVCERIQLNGKP 137
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 26.2 bits (55), Expect = 1.6
Identities = 12/22 (54%), Positives = 17/22 (77%)
Frame = -3
Query: 365 SPDLMDLGLSSVDLPVRRSTFS 300
+PDL D+ LSSVD P++ +T S
Sbjct: 30 APDLADVLLSSVDQPLKVNTCS 51
>SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr
3|||Manual
Length = 396
Score = 26.2 bits (55), Expect = 1.6
Identities = 14/42 (33%), Positives = 22/42 (52%), Gaps = 2/42 (4%)
Frame = -3
Query: 359 DLMDLGLSSVDL--PVRRSTFSLISANLQAMWAVWQSNTGVY 240
+L LG++ + P +RST S ++ L W + N GVY
Sbjct: 316 ELSKLGVTIIGSKDPKKRSTHSYVAKILNPEWDAFLKNEGVY 357
>SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 163
Score = 25.4 bits (53), Expect = 2.8
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -2
Query: 318 TTVDFLFDFGKFAGYVGCVAIQYRCVSV*YLTWV 217
T +D+LF F+ +G + Y ++V Y+ WV
Sbjct: 73 TLIDYLFFSPPFSLSIGPSLLVYLSIAVSYMLWV 106
>SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 462
Score = 24.6 bits (51), Expect = 4.9
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -3
Query: 299 LISANLQAMWAVWQSNTGV 243
L+SA+ W +W +TGV
Sbjct: 280 LVSASFDTTWRLWDVHTGV 298
>SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein
Vps33|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 24.6 bits (51), Expect = 4.9
Identities = 17/49 (34%), Positives = 20/49 (40%)
Frame = +1
Query: 10 TRANITTEVKSVEMPRGSTRSLYPVTMLVSTSKTYLVQRELRRGYVAGD 156
T A I E PR S R Y ML KTY + + G V G+
Sbjct: 158 TDALIDFERTHGRFPRVSGRGPYAAKMLELLEKTYQEEATINFGKVEGE 206
>SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cuf1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 24.2 bits (50), Expect = 6.4
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +1
Query: 211 LNHPGQISNGYTPVLDCHTAHIACK 285
L HP Q+SN +T C A AC+
Sbjct: 305 LPHPIQLSNYFTLPSSCAQADAACQ 329
>SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase
Lac1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 384
Score = 23.8 bits (49), Expect = 8.5
Identities = 8/20 (40%), Positives = 12/20 (60%)
Frame = -3
Query: 287 NLQAMWAVWQSNTGVYPFDI 228
N + MWAVW + + FD+
Sbjct: 295 NFKIMWAVWGTMRTINSFDL 314
>SPAC15E1.05c |||ethanolamine-phosphate cytidylyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 365
Score = 23.8 bits (49), Expect = 8.5
Identities = 12/42 (28%), Positives = 23/42 (54%)
Frame = -3
Query: 359 DLMDLGLSSVDLPVRRSTFSLISANLQAMWAVWQSNTGVYPF 234
+L+D LSSV L + + S++S+ + + + G+ PF
Sbjct: 134 ELLDRLLSSVPLEIYSTPVSVLSSQIDLLRRFATDSDGLTPF 175
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.314 0.130 0.371
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,632,909
Number of Sequences: 5004
Number of extensions: 33271
Number of successful extensions: 71
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 65
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 65
length of database: 2,362,478
effective HSP length: 65
effective length of database: 2,037,218
effective search space used: 114084208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
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