BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0022_O23
(324 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 30 0.075
SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity... 28 0.40
SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66 |... 27 0.70
SPBC25H2.03 |||vacuolar protein involved in phosphoinositide met... 25 2.1
SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces po... 25 2.8
SPAC20H4.08 |||phosphatase activator |Schizosaccharomyces pombe|... 25 2.8
SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 25 3.7
SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccha... 25 3.7
SPAC227.09 |||folylpolyglutamate synthase|Schizosaccharomyces po... 25 3.7
SPBC25H2.11c |||bromodomain protein|Schizosaccharomyces pombe|ch... 24 4.9
SPCC1672.05c |||tyrosine-tRNA ligase |Schizosaccharomyces pombe|... 24 4.9
SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subu... 24 6.5
SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 23 8.6
SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||... 23 8.6
SPBP35G2.10 |mit1||SHREC complex subunit Mit1|Schizosaccharomyce... 23 8.6
SPBC9B6.11c |||CCR4/nocturin family endoribonuclease|Schizosacch... 23 8.6
SPBC27B12.01c |mmm1|SPBC30B4.09c|Mdm10/Mdm12/Mmm1 complex subuni... 23 8.6
SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosacchar... 23 8.6
>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 30.3 bits (65), Expect = 0.075
Identities = 18/47 (38%), Positives = 27/47 (57%), Gaps = 2/47 (4%)
Frame = +2
Query: 80 ANDEPTASEIVGLIEK--TFENSDVIKLKVNKQIMKEDLHRILVSVA 214
+ EP SE+V K T E ++V+ LKV +Q+M+E L V +A
Sbjct: 173 SGSEPAKSELVKEFHKDMTLEEAEVLILKVLRQVMEEKLDSKNVQLA 219
>SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity
factor complex subunit Ctf1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 363
Score = 27.9 bits (59), Expect = 0.40
Identities = 14/41 (34%), Positives = 24/41 (58%), Gaps = 2/41 (4%)
Frame = -2
Query: 254 HNA*LLPPKLHAHQRPIPECGAGLPSLSVY--SLLTLLHRN 138
+N+ +PP LH+ G G+PS ++Y S+ T L++N
Sbjct: 113 YNSRFIPPVLHSTSSLPASQGGGMPSPAIYSSSMATNLNKN 153
>SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 649
Score = 27.1 bits (57), Expect = 0.70
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 2/38 (5%)
Frame = +2
Query: 80 ANDEPTASEIVGLIEKTFENSDVIKL--KVNKQIMKED 187
A DEP S+ VG + N VIK+ + ++I KE+
Sbjct: 486 AEDEPLVSDNVGAVLSLLRNKGVIKVSDEAKEKIQKEE 523
>SPBC25H2.03 |||vacuolar protein involved in phosphoinositide
metabolism|Schizosaccharomyces pombe|chr 2|||Manual
Length = 811
Score = 25.4 bits (53), Expect = 2.1
Identities = 19/56 (33%), Positives = 28/56 (50%), Gaps = 4/56 (7%)
Frame = -3
Query: 193 VQVFLHYLFIHF*LYYIGILKSLLNKPND----LGSRGFVVGHHRQLCRYGVGERI 38
+++ H H + + LKSLL + L SRG ++ RQLC Y GER+
Sbjct: 498 LELLAHIAISHKSVNLVPFLKSLLQMFAEDRKFLNSRGNLI--IRQLCNYIEGERV 551
>SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 2812
Score = 25.0 bits (52), Expect = 2.8
Identities = 11/31 (35%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
Frame = +2
Query: 5 EALPYEKIS-SFDPLSHAVATELTVVANDEP 94
E L + K + S+DP+ + ++T L +DEP
Sbjct: 760 EVLSFVKSNHSYDPIINCISTNLQFTISDEP 790
>SPAC20H4.08 |||phosphatase activator |Schizosaccharomyces pombe|chr
1|||Manual
Length = 270
Score = 25.0 bits (52), Expect = 2.8
Identities = 13/34 (38%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = +2
Query: 83 NDEPTASEIVG-LIEKTFENSDVIKLKVNKQIMK 181
N +P +I L+E+TF+N+DV + K+I K
Sbjct: 134 NKKPVDQKIRSDLLEQTFKNNDVFESADKKKIRK 167
>SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex
subunit Mtr4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1117
Score = 24.6 bits (51), Expect = 3.7
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = -2
Query: 77 PPSALSLRRGREDRMSLSFH 18
PP A S+ +G DR+ +FH
Sbjct: 613 PPVAKSMLKGEADRLDSAFH 632
>SPAC2G11.11c |prh1||ATP-dependent RNA helicase
Prh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 719
Score = 24.6 bits (51), Expect = 3.7
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +2
Query: 197 ILVSVADEHEALEEVAKHYDLKKHNTFYVVQRIPLF 304
+ ++ DE EALE + K Y + + +Q PLF
Sbjct: 309 VFLTGQDEIEALEALIKSYSKQLPSNLPQIQACPLF 344
>SPAC227.09 |||folylpolyglutamate synthase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 417
Score = 24.6 bits (51), Expect = 3.7
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +1
Query: 271 HILRGPKDTSFPGRL 315
H+ G K+TS+PGRL
Sbjct: 261 HVQNGVKNTSWPGRL 275
>SPBC25H2.11c |||bromodomain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 979
Score = 24.2 bits (50), Expect = 4.9
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = +2
Query: 17 YEKISSFDPLSHAVATELTVV 79
Y+ +SSF PL+H V +L +
Sbjct: 687 YDNMSSFKPLAHDVLKKLCTI 707
>SPCC1672.05c |||tyrosine-tRNA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 401
Score = 24.2 bits (50), Expect = 4.9
Identities = 11/65 (16%), Positives = 30/65 (46%)
Frame = +2
Query: 65 ELTVVANDEPTASEIVGLIEKTFENSDVIKLKVNKQIMKEDLHRILVSVADEHEALEEVA 244
++++ PT G + +D +K V+ ++ D+H L ++ E ++
Sbjct: 33 DISLYWGSAPTGRPHCGYFVPMMKLADFLKAGVHVTVLLADVHAFLDNMKAPMELVQHRV 92
Query: 245 KHYDL 259
++Y++
Sbjct: 93 RYYEI 97
>SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1405
Score = 23.8 bits (49), Expect = 6.5
Identities = 12/44 (27%), Positives = 21/44 (47%)
Frame = +2
Query: 23 KISSFDPLSHAVATELTVVANDEPTASEIVGLIEKTFENSDVIK 154
+I+S P ++ + N E E+V + + FEN D+ K
Sbjct: 516 EITSKTPPMTVMSLAFRTLINKEQNKQEVVMISARIFENVDIEK 559
>SPBC29A10.10c |||tRNA-splicing endonuclease positive effector
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1944
Score = 23.4 bits (48), Expect = 8.6
Identities = 14/50 (28%), Positives = 22/50 (44%)
Frame = +2
Query: 47 SHAVATELTVVANDEPTASEIVGLIEKTFENSDVIKLKVNKQIMKEDLHR 196
+H + L + + D P S VG+ E E + K+K I +L R
Sbjct: 1796 THLASNNLNLASRDTPIKSPSVGICE---EKQEAHKVKKRHNIDSANLSR 1842
>SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1260
Score = 23.4 bits (48), Expect = 8.6
Identities = 11/35 (31%), Positives = 20/35 (57%)
Frame = +2
Query: 116 LIEKTFENSDVIKLKVNKQIMKEDLHRILVSVADE 220
+IEK E D+I + + K+ E++ + VA+E
Sbjct: 422 VIEKFSELRDIINIDIEKEKTIEEIAMGFIQVANE 456
>SPBP35G2.10 |mit1||SHREC complex subunit Mit1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1418
Score = 23.4 bits (48), Expect = 8.6
Identities = 14/44 (31%), Positives = 22/44 (50%), Gaps = 1/44 (2%)
Frame = -3
Query: 307 LEKRYPLDHVECVVLLKVIMLSYFLQSFML-ISDRYQNAVQVFL 179
LEKRY + E V L I+ +FL+ + D + V+V +
Sbjct: 745 LEKRYSIIDTEKVTELHQILKPFFLRRVKSEVLDNFPTKVEVII 788
>SPBC9B6.11c |||CCR4/nocturin family
endoribonuclease|Schizosaccharomyces pombe|chr
2|||Manual
Length = 502
Score = 23.4 bits (48), Expect = 8.6
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = -3
Query: 319 EPSVLEKRYPLDHVECVVLLKVI 251
E LE RYP DHV + ++++
Sbjct: 480 EAEPLEGRYPSDHVALMANVQIV 502
>SPBC27B12.01c |mmm1|SPBC30B4.09c|Mdm10/Mdm12/Mmm1 complex subunit
Mmm1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 346
Score = 23.4 bits (48), Expect = 8.6
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = -3
Query: 175 YLFIHF*LYYIGILKSLLNKPNDLGS 98
Y+F+ F L+ I KS+ N P G+
Sbjct: 25 YVFLRFFLFCSPIPKSVANSPKQTGN 50
>SPAC9G1.10c |||inositol polyphosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1191
Score = 23.4 bits (48), Expect = 8.6
Identities = 15/57 (26%), Positives = 24/57 (42%)
Frame = +2
Query: 23 KISSFDPLSHAVATELTVVANDEPTASEIVGLIEKTFENSDVIKLKVNKQIMKEDLH 193
K S P+S + + + PT SE ++ E +VI +K + DLH
Sbjct: 185 KAGSSSPISVSPLKNVKAYISQSPTHSEASSVLSSEEEEENVI--NSSKSVPSFDLH 239
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,355,489
Number of Sequences: 5004
Number of extensions: 24085
Number of successful extensions: 84
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 83
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 84
length of database: 2,362,478
effective HSP length: 64
effective length of database: 2,042,222
effective search space used: 87815546
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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