Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= S06A01NCLL0021_K19
         (329 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   100   5e-23
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    90   7e-20
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    55   3e-09
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    54   4e-09
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   1.3  
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c...    26   1.7  
SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pom...    25   2.2  
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo...    25   2.2  
SPBC36.04 |cys11|cys1a|cysteine synthase |Schizosaccharomyces po...    24   5.2  
SPAC17A2.05 |||fumerate reductase|Schizosaccharomyces pombe|chr ...    24   5.2  
SPBC1718.01 |pop1|ste16, SPBC2G2.18|F-box/WD repeat protein Pop1...    24   6.8  
SPAC6G9.05 |pcd1||coenzyme A diphosphatase |Schizosaccharomyces ...    24   6.8  
SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces pom...    24   6.8  
SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa...    24   6.8  
SPBC947.04 |||DIPSY family|Schizosaccharomyces pombe|chr 2|||Manual    23   9.0  
SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA reductase|Schi...    23   9.0  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  100 bits (240), Expect = 5e-23
 Identities = 44/68 (64%), Positives = 52/68 (76%)
 Frame = +2

Query: 125 HGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKDVPRAVFVDLEPTVVDEVRTGTYRQLF 304
           HGIQP+G M  +      D  F+TFFSETG GK VPR+++VDLEP V+D+VRTG YR LF
Sbjct: 28  HGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLF 87

Query: 305 HPEQLITG 328
           HPEQLITG
Sbjct: 88  HPEQLITG 95



 Score = 31.9 bits (69), Expect = 0.026
 Identities = 11/11 (100%), Positives = 11/11 (100%)
 Frame = +1

Query: 91  IGNACWELYCL 123
           IGNACWELYCL
Sbjct: 16  IGNACWELYCL 26


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 90.2 bits (214), Expect = 7e-20
 Identities = 43/73 (58%), Positives = 53/73 (72%), Gaps = 5/73 (6%)
 Frame = +2

Query: 125 HGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKDVPRAVFVDLEPTVVDEVRTGT 289
           HGI PDG  PT+     K     +D F TFFSETG GK VPR+++VDLEP V+D+VRTG 
Sbjct: 28  HGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGP 86

Query: 290 YRQLFHPEQLITG 328
           Y+ LFHPEQ++TG
Sbjct: 87  YKDLFHPEQMVTG 99



 Score = 31.9 bits (69), Expect = 0.026
 Identities = 11/11 (100%), Positives = 11/11 (100%)
 Frame = +1

Query: 91  IGNACWELYCL 123
           IGNACWELYCL
Sbjct: 16  IGNACWELYCL 26


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 54.8 bits (126), Expect = 3e-09
 Identities = 23/49 (46%), Positives = 32/49 (65%)
 Frame = +2

Query: 182 DSFNTFFSETGAGKDVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITG 328
           +  N +F+E   GK VPRAV VDLEP  +D V++G +  LF P+ +I G
Sbjct: 45  ERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYG 93


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 54.4 bits (125), Expect = 4e-09
 Identities = 25/66 (37%), Positives = 41/66 (62%)
 Frame = +2

Query: 125 HGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKDVPRAVFVDLEPTVVDEVRTGTYRQLF 304
           HGI PDG + +  T   G D  + FF ++   + +PRA+ +DLEP VV+ + + TY  L+
Sbjct: 29  HGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLY 86

Query: 305 HPEQLI 322
           +PE ++
Sbjct: 87  NPENIL 92


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 26.2 bits (55), Expect = 1.3
 Identities = 16/45 (35%), Positives = 25/45 (55%)
 Frame = +3

Query: 123 STASNLMVRCQLTRPSGVETTPSTRSSARPELARTFPVPSSSILN 257
           ST S+L      ++PS   T+ ST SSA P    +  + SSS+++
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSSSYLSSSSVVS 217


>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1274

 Score = 25.8 bits (54), Expect = 1.7
 Identities = 11/27 (40%), Positives = 16/27 (59%)
 Frame = +1

Query: 58  ECSLSVAKPESIGNACWELYCLARHPT 138
           E  ++V KP SI N C+ L  ++  PT
Sbjct: 690 EIPVTVMKPSSISNTCFSLRDVSFEPT 716


>SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 963

 Score = 25.4 bits (53), Expect = 2.2
 Identities = 11/24 (45%), Positives = 15/24 (62%)
 Frame = +3

Query: 165 PSGVETTPSTRSSARPELARTFPV 236
           P+ VETTPST +SA  +    F +
Sbjct: 350 PANVETTPSTATSAPKKSTAPFAI 373


>SPAC17C9.03 |tif471||translation initiation factor eIF4G
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1403

 Score = 25.4 bits (53), Expect = 2.2
 Identities = 11/32 (34%), Positives = 20/32 (62%)
 Frame = +3

Query: 153 QLTRPSGVETTPSTRSSARPELARTFPVPSSS 248
           Q  +PSGVE T ++R++A+ +   T  + S +
Sbjct: 42  QAVKPSGVEPTNTSRANAQKKTESTGKITSEA 73


>SPBC36.04 |cys11|cys1a|cysteine synthase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 351

 Score = 24.2 bits (50), Expect = 5.2
 Identities = 20/73 (27%), Positives = 31/73 (42%), Gaps = 9/73 (12%)
 Frame = +3

Query: 108 GAVLLSTASNLMVR-CQLTRPSGVETT---PSTRSSARPEL-----ARTFPVPSSSILNP 260
           G ++  TA N  +    + R  G +     P+T+S A+ +      A   PVP +   NP
Sbjct: 75  GTIVEGTAGNTGIGLAHIARARGYKCVIYMPNTQSQAKIDTLKFLGAEVHPVPVAPFSNP 134

Query: 261 L*LTRSALAHTDS 299
           L     A  H +S
Sbjct: 135 LNYNHQARRHAES 147


>SPAC17A2.05 |||fumerate reductase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 513

 Score = 24.2 bits (50), Expect = 5.2
 Identities = 11/23 (47%), Positives = 15/23 (65%)
 Frame = +3

Query: 189 STRSSARPELARTFPVPSSSILN 257
           S +S A+PEL RT    SSS ++
Sbjct: 111 SAKSKAKPELLRTLTSKSSSAVD 133


>SPBC1718.01 |pop1|ste16, SPBC2G2.18|F-box/WD repeat protein
           Pop1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 775

 Score = 23.8 bits (49), Expect = 6.8
 Identities = 9/22 (40%), Positives = 14/22 (63%)
 Frame = -1

Query: 173 PRWSCQLASDHQVGCRAKQYSS 108
           P  SC+L S+H+  C  ++ SS
Sbjct: 110 PHLSCELPSEHREKCLKRRNSS 131


>SPAC6G9.05 |pcd1||coenzyme A diphosphatase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 285

 Score = 23.8 bits (49), Expect = 6.8
 Identities = 12/39 (30%), Positives = 20/39 (51%)
 Frame = +3

Query: 213 ELARTFPVPSSSILNPL*LTRSALAHTDSCFIQNNLSLV 329
           E+   F VP +S LNP     S   +TD  +++ N+  +
Sbjct: 218 EVKDLFYVPLTSFLNPKHQKISRFRNTDLLYVEFNIDKI 256


>SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 204

 Score = 23.8 bits (49), Expect = 6.8
 Identities = 9/22 (40%), Positives = 13/22 (59%)
 Frame = -3

Query: 234 RGTSLPAPVSLKNVLKESSPPP 169
           RG ++P P S+ +    S PPP
Sbjct: 43  RGLTIPPPPSVDHSAPPSGPPP 64


>SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 669

 Score = 23.8 bits (49), Expect = 6.8
 Identities = 15/50 (30%), Positives = 25/50 (50%)
 Frame = -2

Query: 322 DKLFWMKQLSVCASADLVNYSGFKIDEDGTGNVLASSGLAEERVEGVVST 173
           D+L  +  L +  SA    + G+  D+D   NV+A  G  ++R E  + T
Sbjct: 330 DQLTPITPLMLALSAGTPAFRGYLADQDCRWNVIA--GAVDDRTEEEMKT 377


>SPBC947.04 |||DIPSY family|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 973

 Score = 23.4 bits (48), Expect = 9.0
 Identities = 15/69 (21%), Positives = 31/69 (44%)
 Frame = -2

Query: 313 FWMKQLSVCASADLVNYSGFKIDEDGTGNVLASSGLAEERVEGVVSTPDGLVSWHLTIRL 134
           F +K  S     D V+Y  F   +DG   +  ++  ++   E  ++ P    S  +++  
Sbjct: 16  FTLKSTSNAEKHDKVDYKDFVRRDDGDTCLRYTTIYSQGEYETTITIPPNSYSNGVSLSN 75

Query: 133 DAVLSNTAP 107
             +LS ++P
Sbjct: 76  GTLLSTSSP 84


>SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA
           reductase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1053

 Score = 23.4 bits (48), Expect = 9.0
 Identities = 17/66 (25%), Positives = 31/66 (46%)
 Frame = +3

Query: 102 LLGAVLLSTASNLMVRCQLTRPSGVETTPSTRSSARPELARTFPVPSSSILNPL*LTRSA 281
           L  +VLLST  ++     L R SGV  +  +   + P L     +  ++ L    +TR +
Sbjct: 235 LFFSVLLSTLFSVQFAMTLVRASGVRISLVSLIESLPFLINVVALDKAAELTRQVITRCS 294

Query: 282 LAHTDS 299
           ++ + S
Sbjct: 295 VSDSHS 300


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,410,327
Number of Sequences: 5004
Number of extensions: 28147
Number of successful extensions: 107
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 91
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 107
length of database: 2,362,478
effective HSP length: 64
effective length of database: 2,042,222
effective search space used: 91899990
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)