Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0021_C04
(469 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 248 2e-67
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 243 1e-65
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 125 3e-30
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 84 9e-18
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 28 0.62
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate... 28 0.82
SPCC1020.01c |pma2|SPCC1393.01|P-type proton ATPase Pma2 |Schizo... 27 1.1
SPAC869.01 |||amidase |Schizosaccharomyces pombe|chr 1|||Manual 25 4.4
SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|c... 25 7.6
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 25 7.6
SPAC23D3.13c |||guanyl-nucleotide exchange factor|Schizosaccharo... 25 7.6
SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr 1... 25 7.6
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 248 bits (608), Expect = 2e-67
Identities = 107/153 (69%), Positives = 130/153 (84%)
Frame = +2
Query: 11 GTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHX 190
G Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL+FH
Sbjct: 85 GPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHS 144
Query: 191 XXXXXXXXXXXLLMERLSVDYGKKSKLECAIYPAPQVSTAVVEPYNSILTTHTTLEHSDC 370
LL+ERL+++YGKKS L+ ++YPAPQVST+VVEPYNS+LTTH TL++SDC
Sbjct: 145 FGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDC 204
Query: 371 AFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQ 469
FMVDNEA YDICRRNLDIERPTY NLNRLI Q
Sbjct: 205 TFMVDNEACYDICRRNLDIERPTYENLNRLIAQ 237
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 243 bits (594), Expect = 1e-65
Identities = 107/153 (69%), Positives = 128/153 (83%)
Frame = +2
Query: 11 GTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHX 190
G YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH
Sbjct: 81 GPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHS 140
Query: 191 XXXXXXXXXXXLLMERLSVDYGKKSKLECAIYPAPQVSTAVVEPYNSILTTHTTLEHSDC 370
LL+ERL+++Y KKSKL+ ++YPAPQVST+VVEPYNS+LTTH TL+ +DC
Sbjct: 141 FGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADC 200
Query: 371 AFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQ 469
FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q
Sbjct: 201 TFMVDNESCYDICRRNLDIERPSYENLNRLIAQ 233
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 125 bits (302), Expect = 3e-30
Identities = 58/153 (37%), Positives = 89/153 (58%)
Frame = +2
Query: 5 EAGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIF 184
++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C LQGF +
Sbjct: 77 KSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLT 136
Query: 185 HXXXXXXXXXXXXLLMERLSVDYGKKSKLECAIYPAPQVSTAVVEPYNSILTTHTTLEHS 364
H LL+ ++ +Y + ++ PAP+ S VVEPYN+ L+ H +E+S
Sbjct: 137 HSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENS 196
Query: 365 DCAFMVDNEAIYDICRRNLDIERPTYTNLNRLI 463
D F +DNEA+ I L I+ P+Y +LN L+
Sbjct: 197 DETFCIDNEALSSIFANTLKIKSPSYDDLNHLV 229
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 84.2 bits (199), Expect = 9e-18
Identities = 49/153 (32%), Positives = 78/153 (50%), Gaps = 3/153 (1%)
Frame = +2
Query: 14 TYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFH 187
TY L++PE ++ K A NN+A G Y+ + I + ++D I + AD L+GF + H
Sbjct: 81 TYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLH 139
Query: 188 XXXXXXXXXXXXLLMERLSVDYGKKSKLECAIYPAPQ-VSTAVVEPYNSILTTHTTLEHS 364
L+ERL+ Y KK +++P Q VS VV+PYNS+L ++
Sbjct: 140 SIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNA 199
Query: 365 DCAFMVDNEAIYDICRRNLDIERPTYTNLNRLI 463
D ++DN A+ I L + PT+ N+L+
Sbjct: 200 DSVVVLDNAALAHIAADRLHTQNPTFHQQNQLV 232
>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1727
Score = 28.3 bits (60), Expect = 0.62
Identities = 21/73 (28%), Positives = 35/73 (47%), Gaps = 1/73 (1%)
Frame = -1
Query: 337 KDRVVGLYNSSGHLGCGIDSALQFRLLAVVNGESLHEERSETRTGATTERVEDEESL-EA 161
KD++ L N + HLG + SA Q + E + + +++ RV++E + E
Sbjct: 146 KDQIEALQNENSHLGEQVQSAHQAL-------SDIEERKKQHMFASSSSRVKEEILVQEK 198
Query: 160 CALVSEFADAVED 122
ALVS+ A D
Sbjct: 199 SALVSDLASLQSD 211
>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
transferase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 453
Score = 27.9 bits (59), Expect = 0.82
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = +3
Query: 366 IVLSWSTMKPSMTSAAVTWILNARPTPTSI 455
++ S+ T KP + TW +N R P S+
Sbjct: 37 VIASYPTFKPVVNERCGTWYVNRRHAPISV 66
>SPCC1020.01c |pma2|SPCC1393.01|P-type proton ATPase Pma2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1010
Score = 27.5 bits (58), Expect = 1.1
Identities = 18/72 (25%), Positives = 35/72 (48%)
Frame = -1
Query: 232 HEERSETRTGATTERVEDEESLEACALVSEFADAVEDQVYDFLSNGVVTTSIVVCGILLT 53
HE+ + +TG ++ +++E ++A D E+QV + S G + VV LL
Sbjct: 108 HEKPARPQTGEGSDNEDEDEDIDALIEDLYSQDQEEEQVEEEESPGPAGAAKVVPEELLE 167
Query: 52 SDKLFWMKQLSV 17
+D + + + V
Sbjct: 168 TDPKYGLTESEV 179
>SPAC869.01 |||amidase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 583
Score = 25.4 bits (53), Expect = 4.4
Identities = 11/24 (45%), Positives = 17/24 (70%), Gaps = 1/24 (4%)
Frame = -1
Query: 136 DAVEDQVYDFLSNGVVT-TSIVVC 68
DA DQ+ +++ NG++T T IV C
Sbjct: 68 DATIDQLQNYMENGILTSTDIVHC 91
>SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 649
Score = 24.6 bits (51), Expect = 7.6
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +2
Query: 56 KEDAANNYARGHYTIGKEIVDLV 124
+E ANN+ RGH I K+ D +
Sbjct: 599 RERKANNFFRGHIEIDKKTKDFL 621
>SPAC1805.15c |pub2||ubiquitin-protein ligase
Pub2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 671
Score = 24.6 bits (51), Expect = 7.6
Identities = 9/23 (39%), Positives = 13/23 (56%)
Frame = +1
Query: 274 VRYLSRTPGVHCCCRALQLYPYN 342
V ++S+ P H C L + PYN
Sbjct: 627 VGHISQLPKAHTCFNRLDIPPYN 649
>SPAC23D3.13c |||guanyl-nucleotide exchange factor|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1616
Score = 24.6 bits (51), Expect = 7.6
Identities = 11/50 (22%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = -1
Query: 235 LHEERSETRTGATTERVEDEESLEACALVSEFADAVEDQVYDFLS-NGVV 89
LH + + + ++ ED+ +C ++S AD + + L+ NG++
Sbjct: 1078 LHSQPIQNVSDVNNKKDEDDHEQTSCLIISGIADVFSENMSLLLNVNGIL 1127
>SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 881
Score = 24.6 bits (51), Expect = 7.6
Identities = 11/31 (35%), Positives = 15/31 (48%)
Frame = -1
Query: 256 AVVNGESLHEERSETRTGATTERVEDEESLE 164
AVVNG S H + + + + ESLE
Sbjct: 662 AVVNGNSQHHHNRDDASSGLSSNGSENESLE 692
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,026,237
Number of Sequences: 5004
Number of extensions: 39898
Number of successful extensions: 132
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 131
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 178394480
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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