Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0020_O18
(386 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 159 2e-40
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|... 46 3e-06
SPAC1A6.05c |||triacylglycerol lipase|Schizosaccharomyces pombe|... 31 0.048
SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1 |Schizosaccharom... 29 0.34
SPAC821.05 |||translation initiation factor eIF3h|Schizosaccharo... 27 1.0
SPAC1B3.03c |wis2|cyp5|cyclophilin family peptidyl-prolyl cis-tr... 26 1.8
SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces po... 25 3.1
SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit Pa... 25 4.1
SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||M... 25 5.5
SPBP8B7.04 |mug45||sequence orphan|Schizosaccharomyces pombe|chr... 24 7.2
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 24 9.6
SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1 |S... 24 9.6
>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 591
Score = 159 bits (385), Expect = 2e-40
Identities = 71/124 (57%), Positives = 91/124 (73%)
Frame = +1
Query: 13 DLITITETMLSGMVKSIHGTYKVKYRPDGPSGEEVEIDFTPPFARVPMIASLEKILNVRL 192
DL+ TE +LSG+VK + G+YKV Y P+GP G + E+DF+ P+ R+ MI LE+ LN +
Sbjct: 353 DLMDTTEELLSGLVKDLTGSYKVPYHPEGPEGPKWELDFSRPWRRINMIEYLEEKLNTKF 412
Query: 193 PSFDTLDTAESNALLRQLCEKHEVECPPPRTTGRVLDKLVGEFLEDKCINPTLILDHPQI 372
P D L T E+NA LR LC KH VEC PP+T R+LDKLVGEF+E +CINPT I+ HPQ+
Sbjct: 413 PPGDQLHTPEANAFLRDLCAKHGVECAPPQTCSRLLDKLVGEFIESECINPTFIIGHPQM 472
Query: 373 MSPL 384
MSPL
Sbjct: 473 MSPL 476
>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 531
Score = 45.6 bits (103), Expect = 3e-06
Identities = 31/121 (25%), Positives = 60/121 (49%), Gaps = 1/121 (0%)
Frame = +1
Query: 25 ITETMLSGMVKSIHGTYKVKYRPDGPSGEEVEIDFTPPFARVPMIASLEKILNVRLPSFD 204
+TE ++ + +I+G ++ SG+ V+++ F + I +L+K LNV L D
Sbjct: 312 LTEELIRFICLTINGNLQI-------SGQTVDLE--KGFEVIEFIPALQKELNVELSPLD 362
Query: 205 TLDTAESNALLRQLCEKHEVECPPPRTTGRVLDKLVGEF-LEDKCINPTLILDHPQIMSP 381
+ + + ++ E+ P T +LDKL L+ +P +++HP++MSP
Sbjct: 363 NSENCRKQLI--SIFKRCEIMLPKTCTVAHLLDKLFDSLVLKYNTSSPKFVINHPEVMSP 420
Query: 382 L 384
L
Sbjct: 421 L 421
>SPAC1A6.05c |||triacylglycerol lipase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 483
Score = 31.5 bits (68), Expect = 0.048
Identities = 13/36 (36%), Positives = 22/36 (61%)
Frame = -1
Query: 251 SHNCLRRAFDSAVSSVSKEGNLTFKIFSKLAIIGTL 144
SHN + R A+ ++E +LTF++F++L I L
Sbjct: 366 SHNWVERCCKKAIDLAAQEVSLTFRLFAELGIFSVL 401
>SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 787
Score = 28.7 bits (61), Expect = 0.34
Identities = 13/40 (32%), Positives = 23/40 (57%)
Frame = +1
Query: 52 VKSIHGTYKVKYRPDGPSGEEVEIDFTPPFARVPMIASLE 171
VK+I T+ +Y+P P+G + + ++ P+I SLE
Sbjct: 510 VKAIMNTFYKQYKPFDPAGNKHDANYDDIIELDPLIGSLE 549
>SPAC821.05 |||translation initiation factor
eIF3h|Schizosaccharomyces pombe|chr 1|||Manual
Length = 357
Score = 27.1 bits (57), Expect = 1.0
Identities = 11/31 (35%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
Frame = -1
Query: 332 LSSRN-SPTNLSRTRPVVLGGGHSTSCFSHN 243
L+S N +P+N+ R P+V+ H +C H+
Sbjct: 176 LNSHNLTPSNVIRELPIVIHNSHLATCLLHS 206
>SPAC1B3.03c |wis2|cyp5|cyclophilin family peptidyl-prolyl cis-trans
isomerase Wis2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 356
Score = 26.2 bits (55), Expect = 1.8
Identities = 10/29 (34%), Positives = 16/29 (55%)
Frame = +1
Query: 247 CEKHEVECPPPRTTGRVLDKLVGEFLEDK 333
C K ++E P P TG L++ ++ DK
Sbjct: 167 CTKDQIEAPKPDVTGDSLEEFPDDYEGDK 195
>SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 811
Score = 25.4 bits (53), Expect = 3.1
Identities = 25/89 (28%), Positives = 38/89 (42%)
Frame = +1
Query: 73 YKVKYRPDGPSGEEVEIDFTPPFARVPMIASLEKILNVRLPSFDTLDTAESNALLRQLCE 252
Y+V P +G++ +I T F ++ S E I + S T + S LC
Sbjct: 427 YRVLNSPHPRTGDKWKIYPTYDFTHC-LVDSFENISH----SLCTTEFILSRVSYEWLCN 481
Query: 253 KHEVECPPPRTTGRVLDKLVGEFLEDKCI 339
EV CP R GR+ +VG + + I
Sbjct: 482 ALEVYCPAQREYGRL--NVVGTLMSKRKI 508
>SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit
Par2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 627
Score = 25.0 bits (52), Expect = 4.1
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +1
Query: 169 EKILNVRLPSFDTLDTAESNALLRQLCEK 255
EK VRLPSFD + T+E L + E+
Sbjct: 201 EKRTLVRLPSFDDVHTSEREELFIKKLEQ 229
>SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1317
Score = 24.6 bits (51), Expect = 5.5
Identities = 16/50 (32%), Positives = 23/50 (46%)
Frame = -1
Query: 284 VLGGGHSTSCFSHNCLRRAFDSAVSSVSKEGNLTFKIFSKLAIIGTLAKG 135
V+GG TS + + +AF + +S NLTF I K G + G
Sbjct: 1085 VVGGNVLTSQRITDTILKAFQACAASQGDTNNLTFGIGGKDPETGEVKPG 1134
>SPBP8B7.04 |mug45||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 819
Score = 24.2 bits (50), Expect = 7.2
Identities = 20/87 (22%), Positives = 35/87 (40%), Gaps = 8/87 (9%)
Frame = +1
Query: 73 YKVKYRPDGPSGEEVEIDFTPPFARVPMIASLEKILNV---RLPSFDTLDTAESNALLRQ 243
+K Y + + ++DFT + L L R+PSF+++ +E++ L
Sbjct: 84 FKHVYSQNVELNHKTQVDFTEQLIPKKSLNKLNGTLRETMSRMPSFESISLSETSNALDD 143
Query: 244 LCEKH-----EVECPPPRTTGRVLDKL 309
C E E PP G L+ +
Sbjct: 144 SCFNDDWITIESEFSPPIDRGMTLEAM 170
>SPAC19A8.08 |upf2||nonsense-mediated decay protein
Upf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1049
Score = 23.8 bits (49), Expect = 9.6
Identities = 11/35 (31%), Positives = 18/35 (51%)
Frame = +1
Query: 145 RVPMIASLEKILNVRLPSFDTLDTAESNALLRQLC 249
++P + SLE + L +D A N L++ LC
Sbjct: 451 KLPSMVSLELTNEMALEFYDLNTKASRNRLIKALC 485
>SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1842
Score = 23.8 bits (49), Expect = 9.6
Identities = 14/50 (28%), Positives = 23/50 (46%)
Frame = +1
Query: 223 SNALLRQLCEKHEVECPPPRTTGRVLDKLVGEFLEDKCINPTLILDHPQI 372
SN L + + H CP + L K +G+ L D C + ++D P +
Sbjct: 439 SNPTLLEFMQYHIDHCPAEKGETYQLAKTLGQQLIDNCKS---VIDAPPV 485
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,649,809
Number of Sequences: 5004
Number of extensions: 32344
Number of successful extensions: 96
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 95
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 96
length of database: 2,362,478
effective HSP length: 65
effective length of database: 2,037,218
effective search space used: 128344734
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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