Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0020_B04
(397 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 154 5e-39
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 154 5e-39
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 154 5e-39
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 48 7e-07
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 27 0.80
SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr 3|||Ma... 26 1.8
SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces pom... 25 3.2
SPAC15E1.05c |||ethanolamine-phosphate cytidylyltransferase |Sch... 25 4.3
SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces p... 25 5.6
SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4 family|S... 25 5.6
SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cu... 24 7.4
SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase Lac... 24 9.8
SPBC646.08c |||oxysterol binding protein |Schizosaccharomyces po... 24 9.8
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 154 bits (373), Expect = 5e-39
Identities = 78/131 (59%), Positives = 94/131 (71%)
Frame = +2
Query: 5 EEAVPGDNVGFNVKNVSVKELRPXPXGGXFWGGXYVLXXEIRKTTHPG*AADFTAQVIVL 184
+ +PGDNVGFNVKNVSVK++R G G K P A FTAQVI+L
Sbjct: 298 DAGLPGDNVGFNVKNVSVKDIRR----GNVCGDS--------KNDPPMGCASFTAQVIIL 345
Query: 185 NHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPS 364
NHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E++PK +KSGDA I +VPS
Sbjct: 346 NHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPS 405
Query: 365 QKPLCVESFQE 397
KP+CVE+F +
Sbjct: 406 -KPMCVEAFTD 415
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 154 bits (373), Expect = 5e-39
Identities = 78/131 (59%), Positives = 94/131 (71%)
Frame = +2
Query: 5 EEAVPGDNVGFNVKNVSVKELRPXPXGGXFWGGXYVLXXEIRKTTHPG*AADFTAQVIVL 184
+ +PGDNVGFNVKNVSVK++R G G K P A FTAQVI+L
Sbjct: 298 DAGLPGDNVGFNVKNVSVKDIRR----GNVCGDS--------KNDPPMGCASFTAQVIIL 345
Query: 185 NHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPS 364
NHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E++PK +KSGDA I +VPS
Sbjct: 346 NHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPS 405
Query: 365 QKPLCVESFQE 397
KP+CVE+F +
Sbjct: 406 -KPMCVEAFTD 415
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 154 bits (373), Expect = 5e-39
Identities = 78/131 (59%), Positives = 94/131 (71%)
Frame = +2
Query: 5 EEAVPGDNVGFNVKNVSVKELRPXPXGGXFWGGXYVLXXEIRKTTHPG*AADFTAQVIVL 184
+ +PGDNVGFNVKNVSVK++R G G K P A FTAQVI+L
Sbjct: 298 DAGLPGDNVGFNVKNVSVKDIRR----GNVCGDS--------KNDPPMGCASFTAQVIIL 345
Query: 185 NHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPS 364
NHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E++PK +KSGDA I +VPS
Sbjct: 346 NHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPS 405
Query: 365 QKPLCVESFQE 397
KP+CVE+F +
Sbjct: 406 -KPMCVEAFTD 415
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 47.6 bits (108), Expect = 7e-07
Identities = 24/79 (30%), Positives = 43/79 (54%)
Frame = +2
Query: 161 FTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDA 340
F AQ+ +L P ++ GY+ V+ HTA FA++ K+D+ KS + P +
Sbjct: 560 FIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLDKTNRKSKK--PPMFATKGM 617
Query: 341 AIVNLVPSQKPLCVESFQE 397
I+ + +Q P+C+E F++
Sbjct: 618 KIIAELETQTPVCMERFED 636
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 27.5 bits (58), Expect = 0.80
Identities = 12/25 (48%), Positives = 19/25 (76%)
Frame = -2
Query: 345 MAASPDLMDLGLSSVDLPVRRSTFS 271
++ +PDL D+ LSSVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
>SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr
3|||Manual
Length = 396
Score = 26.2 bits (55), Expect = 1.8
Identities = 14/42 (33%), Positives = 22/42 (52%), Gaps = 2/42 (4%)
Frame = -2
Query: 330 DLMDLGLSSVDL--PVRRSTFSLISANLQAMWAVWQSNTGVY 211
+L LG++ + P +RST S ++ L W + N GVY
Sbjct: 316 ELSKLGVTIIGSKDPKKRSTHSYVAKILNPEWDAFLKNEGVY 357
>SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 163
Score = 25.4 bits (53), Expect = 3.2
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -1
Query: 289 TTVDFLFDFGKFAGYVGCVAIQYRCVSV*YLTWV 188
T +D+LF F+ +G + Y ++V Y+ WV
Sbjct: 73 TLIDYLFFSPPFSLSIGPSLLVYLSIAVSYMLWV 106
>SPAC15E1.05c |||ethanolamine-phosphate cytidylyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 365
Score = 25.0 bits (52), Expect = 4.3
Identities = 14/55 (25%), Positives = 28/55 (50%)
Frame = -2
Query: 369 FWEGTRLTMAASPDLMDLGLSSVDLPVRRSTFSLISANLQAMWAVWQSNTGVYPF 205
+ E R ++ +L+D LSSV L + + S++S+ + + + G+ PF
Sbjct: 121 YLEVKRTEGVSTTELLDRLLSSVPLEIYSTPVSVLSSQIDLLRRFATDSDGLTPF 175
>SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 505
Score = 24.6 bits (51), Expect = 5.6
Identities = 12/32 (37%), Positives = 17/32 (53%), Gaps = 1/32 (3%)
Frame = +1
Query: 193 RSNIKRIHTCIGLPHSPHSLQIC-RNQRESRP 285
+S + I CIG+ SPH +C R Q +P
Sbjct: 106 KSGERSIPKCIGMYTSPHLRSVCERIQLNGKP 137
>SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 462
Score = 24.6 bits (51), Expect = 5.6
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -2
Query: 270 LISANLQAMWAVWQSNTGV 214
L+SA+ W +W +TGV
Sbjct: 280 LVSASFDTTWRLWDVHTGV 298
>SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cuf1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 24.2 bits (50), Expect = 7.4
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +2
Query: 182 LNHPGQISNGYTPVLDCHTAHIACK 256
L HP Q+SN +T C A AC+
Sbjct: 305 LPHPIQLSNYFTLPSSCAQADAACQ 329
>SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase
Lac1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 384
Score = 23.8 bits (49), Expect = 9.8
Identities = 8/20 (40%), Positives = 12/20 (60%)
Frame = -2
Query: 258 NLQAMWAVWQSNTGVYPFDI 199
N + MWAVW + + FD+
Sbjct: 295 NFKIMWAVWGTMRTINSFDL 314
>SPBC646.08c |||oxysterol binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 516
Score = 23.8 bits (49), Expect = 9.8
Identities = 13/28 (46%), Positives = 16/28 (57%), Gaps = 3/28 (10%)
Frame = +3
Query: 315 LNPLNLV---MPPLSTWFPPKSPCVWSP 389
LN L+LV PPL FP +S +W P
Sbjct: 415 LNELDLVHKRCPPLDKQFPFESRKIWFP 442
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,827,049
Number of Sequences: 5004
Number of extensions: 36081
Number of successful extensions: 90
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 84
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 84
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 132093910
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -