Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0014_J22
(490 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe... 184 6e-48
SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces po... 85 5e-18
SPAPB1A10.11c |||glutamyl-tRNA synthetase, mitochondrial|Schizos... 31 0.12
SPCC297.04c |set7||histone lysine methyltransferase Set7 |Schizo... 26 2.7
SPBC1734.07c |||TRAPP complex subunit Trs85 |Schizosaccharomyces... 26 2.7
SPBC947.12 |kms2||spindle pole body protein Kms2|Schizosaccharom... 26 3.5
SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 26 3.5
SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|... 25 6.1
SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog |Schizosacchar... 25 6.1
>SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 716
Score = 184 bits (448), Expect = 6e-48
Identities = 80/148 (54%), Positives = 109/148 (73%)
Frame = +1
Query: 46 DDTPAEQMKAERDQKIESKNRNNSVEKNLKLWEEMKKGSDLGVQCCVRAKIDMQSMNGCL 225
DDT E M+ ER + I SK+R+ +E++L++ EM KGSD+G++ C+RAKI ++ N +
Sbjct: 301 DDTDVETMRHERTEGIPSKHRDRPIEESLEILSEMDKGSDVGLKNCIRAKISYENPNKAM 360
Query: 226 RDPTIYRCKPETHPRTGNQYKVYPTYDFACPIVDSVEGVTHVLRTMEYHDRDPQFYWFIE 405
RDP IYRC H RTG +Y+ YPTYDFACPIVDS+EGVTH LRT EY DR+P + W I+
Sbjct: 361 RDPVIYRCNLLPHHRTGTKYRAYPTYDFACPIVDSLEGVTHALRTTEYRDRNPLYQWMIK 420
Query: 406 AIGLRKPYIWEYSRLSMTNTV*STRKLT 489
A+ LRK ++WE+SR++ T+ S RKLT
Sbjct: 421 AMNLRKIHVWEFSRMNFVRTLLSKRKLT 448
>SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 811
Score = 85.0 bits (201), Expect = 5e-18
Identities = 50/149 (33%), Positives = 77/149 (51%), Gaps = 4/149 (2%)
Frame = +1
Query: 52 TPAEQMKA----ERDQKIESKNRNNSVEKNLKLWEEMKKGSDLGVQCCVRAKIDMQSMNG 219
T AE KA ER + +R+ +E++L + M+ G + +R K D+ N
Sbjct: 360 TDAEIKKARGGEERGPRYACVHRDRPIEESLLEFRNMRDGKYQPKEAILRMKQDLSDGNP 419
Query: 220 CLRDPTIYRCKPETHPRTGNQYKVYPTYDFACPIVDSVEGVTHVLRTMEYHDRDPQFYWF 399
+ D YR HPRTG+++K+YPTYDF +VDS E ++H L T E+ + W
Sbjct: 420 QMWDLIAYRVLNSPHPRTGDKWKIYPTYDFTHCLVDSFENISHSLCTTEFILSRVSYEWL 479
Query: 400 IEAIGLRKPYIWEYSRLSMTNTV*STRKL 486
A+ + P EY RL++ T+ S RK+
Sbjct: 480 CNALEVYCPAQREYGRLNVVGTLMSKRKI 508
>SPAPB1A10.11c |||glutamyl-tRNA synthetase,
mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
Length = 526
Score = 30.7 bits (66), Expect = 0.12
Identities = 15/45 (33%), Positives = 23/45 (51%)
Frame = +1
Query: 292 YPTYDFACPIVDSVEGVTHVLRTMEYHDRDPQFYWFIEAIGLRKP 426
+PTY FA + D + +THV+R E+ + EA G + P
Sbjct: 219 FPTYHFANVVDDHLMHITHVIRGEEWVPSTIKHIQLYEAFGWKPP 263
>SPCC297.04c |set7||histone lysine methyltransferase Set7
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 147
Score = 26.2 bits (55), Expect = 2.7
Identities = 16/59 (27%), Positives = 25/59 (42%)
Frame = +1
Query: 220 CLRDPTIYRCKPETHPRTGNQYKVYPTYDFACPIVDSVEGVTHVLRTMEYHDRDPQFYW 396
C+ + E + + G QY V Y + + +G+ L +M HDR P YW
Sbjct: 33 CIEISPVLMFSKEEYEQHG-QYTVLNEYTYVWS--EGKQGLALGLGSMFNHDRHPNVYW 88
>SPBC1734.07c |||TRAPP complex subunit Trs85 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 618
Score = 26.2 bits (55), Expect = 2.7
Identities = 11/31 (35%), Positives = 19/31 (61%), Gaps = 2/31 (6%)
Frame = -1
Query: 181 STVRLNRYLSSFPPTVLNSSLQNYFYS--CF 95
+T+R+N Y+ S LN S +Y+++ CF
Sbjct: 457 NTLRINEYMQSAMLNYLNKSFNSYYHAARCF 487
>SPBC947.12 |kms2||spindle pole body protein
Kms2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 457
Score = 25.8 bits (54), Expect = 3.5
Identities = 19/67 (28%), Positives = 27/67 (40%)
Frame = +1
Query: 61 EQMKAERDQKIESKNRNNSVEKNLKLWEEMKKGSDLGVQCCVRAKIDMQSMNGCLRDPTI 240
E AE K E N+ +N KL EE+ + C +AK + LRD
Sbjct: 178 ELQAAELRSKDEQVKELNA--RNAKLLEELDSSEEACKSCYTQAKTWEKKFREALRDSKE 235
Query: 241 YRCKPET 261
Y + +T
Sbjct: 236 YAAQLQT 242
>SPBC12C2.02c |ste20|ste16|sterility protein
Ste20|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1309
Score = 25.8 bits (54), Expect = 3.5
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = -1
Query: 232 DLSNNHS*TACLFLHEHSTVRLNRYLSSFPPTVLN 128
D SNN C +LHEHS + S PT+L+
Sbjct: 235 DSSNN----LCEYLHEHSDPAYAKNFSLITPTILS 265
>SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1402
Score = 25.0 bits (52), Expect = 6.1
Identities = 8/19 (42%), Positives = 14/19 (73%)
Frame = -3
Query: 485 NFLVDYTVFVILNLLYSQM 429
N+L DYT ++ NL++S +
Sbjct: 1096 NYLFDYTYVMLFNLIFSSL 1114
>SPCC11E10.09c ||SPCC188.01c|alpha-amylase homolog
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 478
Score = 25.0 bits (52), Expect = 6.1
Identities = 13/34 (38%), Positives = 17/34 (50%)
Frame = -2
Query: 318 GTCKIIRRVYFILVSSAWMSFWFTPIYSRISQTT 217
GT K I R + S + W +PI IS+TT
Sbjct: 51 GTWKGITRNLDYIKSLGCTAIWISPIVKNISETT 84
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,172,074
Number of Sequences: 5004
Number of extensions: 47245
Number of successful extensions: 150
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 190087364
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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