Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0014_D14
(625 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 259 3e-70
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 128 9e-31
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 125 6e-30
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 85 1e-17
SPAC22F8.07c |rtf1||replication termination factor Rtf1|Schizosa... 26 3.8
SPBC13E7.06 |msd1|mug172|spindle pole body protein Msd1|Schizosa... 26 5.1
SPAC25H1.05 |meu29||sequence orphan|Schizosaccharomyces pombe|ch... 25 6.7
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 259 bits (634), Expect = 3e-70
Identities = 117/156 (75%), Positives = 137/156 (87%)
Frame = +3
Query: 15 TYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQ 194
+Y DLNHLVS M+GVTT RFPG+LN+DLRKLAVNMVPFPRLHFFM GFAPL + GS
Sbjct: 221 SYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSS 280
Query: 195 YRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLSIQNKNSSY 374
++A++VPELTQQMFDA NMM A DPRHGRYLTVAA+FRG++SMKEVDEQ+ S+Q KNS+Y
Sbjct: 281 FQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAY 340
Query: 375 FVEWIPNNVKTAVCDIPPKGLKMSSTFIGNTTAIQE 482
FVEWIP+NV AVC +PPK LKMS+TFIGN+T+IQE
Sbjct: 341 FVEWIPDNVLKAVCSVPPKDLKMSATFIGNSTSIQE 376
Score = 89.0 bits (211), Expect = 5e-19
Identities = 38/45 (84%), Positives = 42/45 (93%)
Frame = +2
Query: 491 KRISEQFSAMFRRNAFLHWYTGEGMDEMEFNEAESNVNDLVSEYQ 625
+R+ +QFSAMFRR AFLHWYTGEGMDEMEF EAESN+NDLVSEYQ
Sbjct: 379 RRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQ 423
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 128 bits (308), Expect = 9e-31
Identities = 60/167 (35%), Positives = 95/167 (56%), Gaps = 8/167 (4%)
Frame = +3
Query: 9 RGTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGS 188
R +Y +LN L++ +S +T LRF G LN DL + N+VP+PR+HF + +AP+ S
Sbjct: 221 RPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAK 280
Query: 189 QQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLSIQNKNS 368
+ + +V E+T Q F+ N M CDPR GRY+ ++RG + ++V + +I+ K +
Sbjct: 281 AFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRT 340
Query: 369 SYFVEWIPNNVKTAVCDIPPKGLKMSS--------TFIGNTTAIQEA 485
FV+W P K +CD PP+ ++ S + NTT+I EA
Sbjct: 341 IQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEA 387
Score = 50.8 bits (116), Expect = 2e-07
Identities = 19/44 (43%), Positives = 30/44 (68%)
Frame = +2
Query: 494 RISEQFSAMFRRNAFLHWYTGEGMDEMEFNEAESNVNDLVSEYQ 625
R+ +F M+ + AF+HWY GEGM+E EF+EA ++ L +Y+
Sbjct: 390 RLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYE 433
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 125 bits (301), Expect = 6e-30
Identities = 59/167 (35%), Positives = 94/167 (56%), Gaps = 8/167 (4%)
Frame = +3
Query: 9 RGTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGS 188
R TY +LN L++ +S +T LRF G LN DL + N+VP+PR+HF + ++P+ S
Sbjct: 225 RPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAK 284
Query: 189 QQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLSIQNKNS 368
+ + +V E+T Q F+ N M CDPR GRY+ ++RG + ++V + SI+++ +
Sbjct: 285 AFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRT 344
Query: 369 SYFVEWIPNNVKTAVCDIPPKGLKMSS--------TFIGNTTAIQEA 485
FV+W P K +C PP+ + S + NTT+I EA
Sbjct: 345 IQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEA 391
Score = 50.8 bits (116), Expect = 2e-07
Identities = 19/44 (43%), Positives = 30/44 (68%)
Frame = +2
Query: 494 RISEQFSAMFRRNAFLHWYTGEGMDEMEFNEAESNVNDLVSEYQ 625
R+ +F M+ + AF+HWY GEGM+E EF+EA ++ L +Y+
Sbjct: 394 RLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYE 437
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 84.6 bits (200), Expect = 1e-17
Identities = 44/161 (27%), Positives = 87/161 (54%), Gaps = 7/161 (4%)
Frame = +3
Query: 15 TYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQ 194
T+ N LVS MS TT LR+PG +N DL + +++P PR HF + + P T++ ++
Sbjct: 224 TFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVEE 283
Query: 195 YRAL---TVPELTQQMFDAKNMMAACDP-RHGRYLTVAAIFRGRMSMKEVDEQMLSIQNK 362
+A+ TV ++ +++ KN M + +P + ++++ I +G +V + +L I+ +
Sbjct: 284 AKAIRKTTVLDVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRER 343
Query: 363 NSSYFVEWIPNNVKTAVCDIPP---KGLKMSSTFIGNTTAI 476
+ F+ W P +++ A+ P ++S + N T+I
Sbjct: 344 RYASFIPWGPASIQVALSKKSPYIKTNHRVSGLMLANHTSI 384
Score = 40.7 bits (91), Expect = 2e-04
Identities = 21/57 (36%), Positives = 34/57 (59%), Gaps = 3/57 (5%)
Frame = +2
Query: 464 HHSHPGSCLKRISEQFSAMFRRNAFLHWYTGEGMDEMEFNEAESN---VNDLVSEYQ 625
+H+ S KR +Q+ + +RNAFL Y E + E + NE +S+ V DL++EY+
Sbjct: 380 NHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSSRDVVADLINEYE 436
>SPAC22F8.07c |rtf1||replication termination factor
Rtf1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 466
Score = 26.2 bits (55), Expect = 3.8
Identities = 16/67 (23%), Positives = 26/67 (38%)
Frame = -3
Query: 491 LNSFLDGCGVTDECRGHLEAFRRDVTHGGLYVIRDPFHEVR*VLVLN*EHLLIDFLHRHA 312
L+ + + D C+ H E ++ + GL D + +L + E I LH
Sbjct: 397 LSEYANHLWTADACKSHFERIKKTLFIDGLSTFSDTLIHLHKMLNSSPEETYISNLHDSY 456
Query: 311 TAEDGCD 291
TA D
Sbjct: 457 TAFSNAD 463
>SPBC13E7.06 |msd1|mug172|spindle pole body protein
Msd1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 331
Score = 25.8 bits (54), Expect = 5.1
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = -2
Query: 603 LTLLSASLNSISSMPSPVYQ 544
LT LS +LNS+ M SP+Y+
Sbjct: 234 LTCLSGTLNSLVEMLSPLYE 253
>SPAC25H1.05 |meu29||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 217
Score = 25.4 bits (53), Expect = 6.7
Identities = 10/33 (30%), Positives = 20/33 (60%)
Frame = +2
Query: 509 FSAMFRRNAFLHWYTGEGMDEMEFNEAESNVND 607
F A+F N + + Y+ + + ++F+ SNV+D
Sbjct: 11 FFALFIGNTYAYTYSLDRIQALKFSSESSNVDD 43
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,653,649
Number of Sequences: 5004
Number of extensions: 52488
Number of successful extensions: 175
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 275671126
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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