Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0013_O06
(372 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 183 9e-48
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 105 2e-24
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 99 2e-22
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 93 1e-20
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 28 0.54
SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase |Schiz... 27 0.71
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 0.94
SPAC1805.16c |||purine nucleoside phosphorylase |Schizosaccharom... 26 1.6
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 26 1.6
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 26 2.2
SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr 2|... 25 2.9
SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p... 25 5.0
SPCC74.02c |||mRNA cleavage and polyadenylation specificity fact... 25 5.0
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 183 bits (445), Expect = 9e-48
Identities = 77/103 (74%), Positives = 90/103 (87%)
Frame = +2
Query: 62 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 241
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 242 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAK 370
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAK
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAK 103
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 105 bits (252), Expect = 2e-24
Identities = 44/103 (42%), Positives = 72/103 (69%), Gaps = 2/103 (1%)
Frame = +2
Query: 65 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 244
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 245 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWA 367
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWA 105
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 99.1 bits (236), Expect = 2e-22
Identities = 45/105 (42%), Positives = 64/105 (60%), Gaps = 2/105 (1%)
Frame = +2
Query: 62 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 235
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 236 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAK 370
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYAR 105
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 93.5 bits (222), Expect = 1e-20
Identities = 43/109 (39%), Positives = 63/109 (57%), Gaps = 6/109 (5%)
Frame = +2
Query: 62 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 223
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 224 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAK 370
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYAR 109
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 27.9 bits (59), Expect = 0.54
Identities = 16/49 (32%), Positives = 27/49 (55%)
Frame = +3
Query: 72 SCTSRPDNAETRLELSSGRSSPMSTASTPPVLTTATQTCSWSASMFITM 218
S +SR + + LSS S P+ TAS+ +LT+++ T S +T+
Sbjct: 427 STSSRSSSHSSSHSLSSLSSKPILTASSSSLLTSSSHTYERSTVYVVTV 475
>SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 603
Score = 27.5 bits (58), Expect = 0.71
Identities = 11/39 (28%), Positives = 24/39 (61%)
Frame = -3
Query: 148 AVLIGDDLPELSSNLVSALSGLDVHDFPHFDISELNKLL 32
+++I D+L S + LSG ++ + H D+++L ++L
Sbjct: 273 SLIISDELNHTSIRFGARLSGANIRVYKHNDMTDLERVL 311
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 0.94
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 101 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 202
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC1805.16c |||purine nucleoside phosphorylase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 315
Score = 26.2 bits (55), Expect = 1.6
Identities = 11/35 (31%), Positives = 19/35 (54%)
Frame = -3
Query: 148 AVLIGDDLPELSSNLVSALSGLDVHDFPHFDISEL 44
A++ G L L+S L + + + D PHF +S +
Sbjct: 44 AIICGSGLGTLASGLSAPVYEVPYEDIPHFHVSHV 78
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.2 bits (55), Expect = 1.6
Identities = 10/25 (40%), Positives = 15/25 (60%), Gaps = 1/25 (4%)
Frame = +2
Query: 296 GPFGQIFRPDNFVFGQSGAG-NNWA 367
GP+G +F P F+F +G NW+
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWS 181
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.8 bits (54), Expect = 2.2
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -1
Query: 354 PAPDCPKTKLSGRKICPKGPERTESMVPGSRS 259
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
>SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr
2|||Manual
Length = 997
Score = 25.4 bits (53), Expect = 2.9
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = -3
Query: 157 GVDAVLIGDDLPELSSNLVSALSGLDVHDFPHFDISELN 41
G+D +IG+D E S L S L+ LD P++ I N
Sbjct: 176 GIDIRIIGNDAGEKLSILSSTLARLD-RPAPNYGIDNYN 213
>SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1323
Score = 24.6 bits (51), Expect = 5.0
Identities = 18/78 (23%), Positives = 28/78 (35%)
Frame = -3
Query: 265 EVDEDGAGHVLASGGLIVINIDXXXXXXXXXXVSTGGVDAVLIGDDLPELSSNLVSALSG 86
+VDE +G ++ IN++ S G + + SN S L
Sbjct: 911 DVDESNSGSIVFPKSKTSINLNNINSASASIMGSRFGAADIFMSKRPSTKGSNRPSILLS 970
Query: 85 LDVHDFPHFDISELNKLL 32
HDF I ++K L
Sbjct: 971 KPSHDFHVVRIISMSKYL 988
>SPCC74.02c |||mRNA cleavage and polyadenylation specificity factor
complex associated protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 710
Score = 24.6 bits (51), Expect = 5.0
Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = -3
Query: 163 TGGVDAVLIGDDLPE-LSSNLVSALSGLDVHDFPH 62
+G + + + DL E L L+SA GL+ H F H
Sbjct: 31 SGKPEFINLSPDLEENLDEKLMSAFPGLEPHVFQH 65
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,276,211
Number of Sequences: 5004
Number of extensions: 22797
Number of successful extensions: 95
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 91
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 92
length of database: 2,362,478
effective HSP length: 65
effective length of database: 2,037,218
effective search space used: 118158644
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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