Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0013_M15
(472 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 227 5e-61
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 128 3e-31
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 124 9e-30
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 118 5e-28
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 1.4
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 1.9
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 1.9
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 5.8
SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyc... 25 5.8
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 227 bits (556), Expect = 5e-61
Identities = 97/128 (75%), Positives = 114/128 (89%)
Frame = +1
Query: 88 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 267
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 268 PRAILVDLEPGTMDSVRSGPFGPIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 447
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 448 RKESESCD 471
R+E+E+CD
Sbjct: 121 RREAEACD 128
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 128 bits (310), Expect = 3e-31
Identities = 58/129 (44%), Positives = 82/129 (63%), Gaps = 2/129 (1%)
Frame = +1
Query: 88 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 261
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 262 YVPRAILVDLEPGTMDSVRSGPFGPIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 441
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 442 VVRKESESC 468
+R+ +++C
Sbjct: 121 KIRRIADNC 129
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 124 bits (298), Expect = 9e-30
Identities = 55/133 (41%), Positives = 83/133 (62%), Gaps = 6/133 (4%)
Frame = +1
Query: 88 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 249
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 250 SGGKYVPRAILVDLEPGTMDSVRSGPFGPIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 429
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 430 SVLDVVRKESESC 468
SVL+ +R+ +++C
Sbjct: 121 SVLERIRRMADNC 133
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 118 bits (284), Expect = 5e-28
Identities = 49/129 (37%), Positives = 87/129 (67%), Gaps = 2/129 (1%)
Frame = +1
Query: 91 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 270
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 271 RAILVDLEPGTMDSVRSGPFGPIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 444
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 445 VRKESESCD 471
+ +E++ D
Sbjct: 122 IDREADGSD 130
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 1.4
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 127 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 228
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 26.6 bits (56), Expect = 1.9
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +1
Query: 391 AKGHYTEGAELVDSVLDVVRKESES 465
A+GH G ELV + D +RK+SE+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSEN 207
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 1.9
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +1
Query: 322 GPFGPIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 423
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.0 bits (52), Expect = 5.8
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -3
Query: 380 PAPDCPKTKLSGRKIGPKGPERTESMVPGSRS 285
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
>SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 781
Score = 25.0 bits (52), Expect = 5.8
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = -3
Query: 338 IGPKGPERTESMVPGSRSTRMARGTYLPPEASL 240
IG + PER++S P SRS + GT P L
Sbjct: 10 IGSQAPERSDSESPSSRS--IGSGTPAPVRKGL 40
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.317 0.137 0.417
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,740,511
Number of Sequences: 5004
Number of extensions: 32152
Number of successful extensions: 85
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 80
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 82
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 180421690
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
- SilkBase 1999-2023 -