BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0013_H13
(436 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein L22|Schizosa... 68 5e-13
SPBC530.12c |pdf1||palmitoyl protein thioesterase-dolichol pyrop... 30 0.18
SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit Vps23|Schizosac... 26 2.2
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|... 25 3.8
SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ... 25 5.0
SPBC27.01c |||secretory pathway protein Pga2 |Schizosaccharomyce... 24 8.8
SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces... 24 8.8
SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II comp... 24 8.8
>SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein
L22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 117
Score = 68.1 bits (159), Expect = 5e-13
Identities = 33/64 (51%), Positives = 44/64 (68%), Gaps = 1/64 (1%)
Frame = +3
Query: 165 LTCTHPAEDSILDVGNFEKYLKERVTVEGKTNNLGNHVVIARD-KTKVAINADIPFSQRY 341
+ T D I DV FEKYL +R+ V+GKT NLG+ VV++R+ +K+A+ A I FS RY
Sbjct: 14 IDATAAVNDKIFDVAAFEKYLIDRIKVDGKTGNLGSSVVVSREGSSKIAVIAHIDFSGRY 73
Query: 342 LKYL 353
LKYL
Sbjct: 74 LKYL 77
Score = 34.7 bits (76), Expect = 0.006
Identities = 13/19 (68%), Positives = 19/19 (100%)
Frame = +1
Query: 352 LTKRYLRKNNLRDWLRVVA 408
LTK++L+K++LRDWLRVV+
Sbjct: 77 LTKKFLKKHSLRDWLRVVS 95
>SPBC530.12c |pdf1||palmitoyl protein thioesterase-dolichol
pyrophosphate phosphatase fusion 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 603
Score = 29.9 bits (64), Expect = 0.18
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -2
Query: 201 PGCYPQQGVCKSMVNLRLNLPSWH 130
PGC P +CK++V+ L L WH
Sbjct: 141 PGCSPTNLICKAVVHSILGLGIWH 164
>SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit
Vps23|Schizosaccharomyces pombe|chr 1|||Manual
Length = 487
Score = 26.2 bits (55), Expect = 2.2
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = +3
Query: 330 SQRYLKYLDQALPQEKQSSGLAPSGGLLHHDS 425
S+R LKY + EK G+ LHH+S
Sbjct: 428 SERELKYYELKRKDEKLDEGIRALNQALHHES 459
>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 531
Score = 25.4 bits (53), Expect = 3.8
Identities = 14/52 (26%), Positives = 22/52 (42%), Gaps = 5/52 (9%)
Frame = -1
Query: 211 LPTSRMLSSAGCVQVNGKFKAKSS-----LLAPLPRILPPCTPFLPVFWCNW 71
+P + L C+Q +G S LP++L PC +PV N+
Sbjct: 132 IPRLKALQKGDCIQCSGNVGRSGSGELSIYATELPKLLSPCLHPIPVKLTNY 183
>SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor
Ste6|Schizosaccharomyces pombe|chr 3|||Manual
Length = 911
Score = 25.0 bits (52), Expect = 5.0
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -1
Query: 109 PCTPFLPVFWCNWPFFATG 53
PC PFL V++ + F TG
Sbjct: 809 PCVPFLGVYFTDLTFLKTG 827
>SPBC27.01c |||secretory pathway protein Pga2 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 132
Score = 24.2 bits (50), Expect = 8.8
Identities = 6/16 (37%), Positives = 13/16 (81%)
Frame = +1
Query: 364 YLRKNNLRDWLRVVAF 411
YL+ +L+DW+R++ +
Sbjct: 8 YLQSYSLKDWIRIIVY 23
>SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 815
Score = 24.2 bits (50), Expect = 8.8
Identities = 8/22 (36%), Positives = 14/22 (63%)
Frame = +1
Query: 10 SATRVLLPRWQLQRSPSQRKAS 75
+ATR ++ W LQ +P + A+
Sbjct: 500 AATRCIVDTWMLQHAPQSKSAN 521
>SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II complex
subunit Rpb2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1210
Score = 24.2 bits (50), Expect = 8.8
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +3
Query: 327 FSQRYLKYLDQALPQEKQSSGLAP 398
FSQ YL Y + L QE S +AP
Sbjct: 1179 FSQVYLPYAAKLLFQELMSMNIAP 1202
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,895,121
Number of Sequences: 5004
Number of extensions: 37709
Number of successful extensions: 121
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 118
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 120
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 156095170
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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