BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0013_E03
(281 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC23G7.05 |sui1|psu1|translation initiation factor eIF1|Schizo... 41 3e-05
SPAC14C4.03 |mek1||Cds1/Rad53/Chk2 family protein kinase Mek1 |S... 26 1.2
SPAC630.05 |gyp7||GTPase activating protein Gyp7 |Schizosaccharo... 25 2.7
SPAPYUG7.02c |sin1||stress activated MAP kinase interacting prot... 25 2.7
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 24 3.6
SPBC211.02c |cwf3|syf1|complexed with Cdc5 protein Cwf3 |Schizos... 24 3.6
SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch... 24 4.7
SPBP8B7.07c |set6||histone lysine methyltransferase Set6 |Schizo... 23 6.3
SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase Ubp8|Schizos... 23 6.3
SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz... 23 8.3
SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces pomb... 23 8.3
>SPBC23G7.05 |sui1|psu1|translation initiation factor
eIF1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 109
Score = 41.1 bits (92), Expect = 3e-05
Identities = 22/42 (52%), Positives = 27/42 (64%), Gaps = 1/42 (2%)
Frame = +1
Query: 157 SIQNLNTFHPFADAIKSSEDDVQD-GLLHVRIQQWNGRNTST 279
+IQN NT PFAD + +DD Q +H+RIQQ NGR T T
Sbjct: 3 AIQNFNTVDPFAD---TGDDDAQPLNNIHIRIQQRNGRKTLT 41
>SPAC14C4.03 |mek1||Cds1/Rad53/Chk2 family protein kinase Mek1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 445
Score = 25.8 bits (54), Expect = 1.2
Identities = 9/17 (52%), Positives = 13/17 (76%)
Frame = +2
Query: 227 MVYCTSESSSGTGVTHR 277
+VYC +SS+GT + HR
Sbjct: 98 LVYCEDQSSNGTFLNHR 114
>SPAC630.05 |gyp7||GTPase activating protein Gyp7
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 743
Score = 24.6 bits (51), Expect = 2.7
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = -3
Query: 198 SIGEWVECVQILDGHTIERRCSLF 127
SI EWVE Q LD R CS++
Sbjct: 102 SIREWVEIPQHLDYSFSVRLCSIY 125
>SPAPYUG7.02c |sin1||stress activated MAP kinase interacting protein
Sin1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 665
Score = 24.6 bits (51), Expect = 2.7
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = +1
Query: 100 SALYRSVSLKQRASTFNRMSIQNLNTFHPF 189
+++Y S+ ++ST+N I + NT+ F
Sbjct: 533 NSVYGSIKKDAQSSTYNATDIMSSNTYQEF 562
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 24.2 bits (50), Expect = 3.6
Identities = 7/18 (38%), Positives = 12/18 (66%)
Frame = +3
Query: 156 VHPESEHIPPIRRCYQKL 209
+HP + +RRC+Q+L
Sbjct: 1969 IHPLKRKVAQVRRCFQEL 1986
>SPBC211.02c |cwf3|syf1|complexed with Cdc5 protein Cwf3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 790
Score = 24.2 bits (50), Expect = 3.6
Identities = 10/31 (32%), Positives = 19/31 (61%)
Frame = -3
Query: 216 VLRAFDSIGEWVECVQILDGHTIERRCSLFQ 124
+LR S+ W+ ++ +G T+E+R LF+
Sbjct: 25 LLRDPYSLKSWLRYIKTHEGSTLEKRVLLFE 55
>SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit
Rev3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1480
Score = 23.8 bits (49), Expect = 4.7
Identities = 12/56 (21%), Positives = 21/56 (37%)
Frame = +3
Query: 84 TVILLKCVVSQRFIETKSIYVQSYVHPESEHIPPIRRCYQKLGGRRARWSTARPNP 251
T + + V + F+ + + + + IPP+ R LG W P P
Sbjct: 1298 TTLANRSVAPEEFLSSSFSQLDINYYINNSLIPPLDRFLNLLGASAQSWYHEMPKP 1353
>SPBP8B7.07c |set6||histone lysine methyltransferase Set6
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 483
Score = 23.4 bits (48), Expect = 6.3
Identities = 10/43 (23%), Positives = 22/43 (51%)
Frame = -3
Query: 225 LHVVLRAFDSIGEWVECVQILDGHTIERRCSLFQ*NAAIQRTS 97
+++V +FDS+G ++ + H+ + C + A +Q S
Sbjct: 171 MNLVTSSFDSLGMCLDTILCRLNHSCDPNCQIIFDGAIVQLVS 213
>SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase
Ubp8|Schizosaccharomyces pombe|chr 1|||Manual
Length = 449
Score = 23.4 bits (48), Expect = 6.3
Identities = 11/30 (36%), Positives = 14/30 (46%)
Frame = -3
Query: 222 HVVLRAFDSIGEWVECVQILDGHTIERRCS 133
H+ L+ D C QI H + RRCS
Sbjct: 10 HLKLKPADVENYQKICTQIFSCHFVPRRCS 39
>SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1253
Score = 23.0 bits (47), Expect = 8.3
Identities = 9/21 (42%), Positives = 12/21 (57%)
Frame = -3
Query: 213 LRAFDSIGEWVECVQILDGHT 151
+ A+ S G W EC+ IL T
Sbjct: 963 IEAYKSAGMWRECLSILQQTT 983
>SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 605
Score = 23.0 bits (47), Expect = 8.3
Identities = 9/18 (50%), Positives = 10/18 (55%)
Frame = -3
Query: 180 ECVQILDGHTIERRCSLF 127
EC+ IL GHT C F
Sbjct: 344 ECISILHGHTDSVLCLTF 361
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,255,986
Number of Sequences: 5004
Number of extensions: 22258
Number of successful extensions: 58
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 57
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 57
length of database: 2,362,478
effective HSP length: 62
effective length of database: 2,052,230
effective search space used: 63619130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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