Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0012_N05
(477 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 153 1e-38
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 153 1e-38
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 153 1e-38
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 45 5e-06
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 29 0.48
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 27 1.1
SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr 3|||Ma... 27 1.9
SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces p... 26 2.6
SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces pom... 25 4.5
SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces ... 25 7.9
SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4 family|S... 25 7.9
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 153 bits (372), Expect = 1e-38
Identities = 75/145 (51%), Positives = 98/145 (67%)
Frame = +1
Query: 43 PWVELKLGILKPGTVVVFAPANIPY*SQVPWENAPRGSTRSCTR*QMLVSTSKTYLSRNL 222
P ++ G++KPG +V FAPA + ++V S + + K +++
Sbjct: 261 PVGRVETGVIKPGMIVTFAPAGVT--TEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDI 318
Query: 223 RRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVD 402
RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+D
Sbjct: 319 RRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKID 378
Query: 403 RRTGKSTEDNPKSIKSGDADIVNLV 477
RR+GK E++PK +KSGDA I +V
Sbjct: 379 RRSGKKIEESPKFVKSGDACIAKMV 403
Score = 63.7 bits (148), Expect = 1e-11
Identities = 42/88 (47%), Positives = 46/88 (52%)
Frame = +3
Query: 6 RDVYKIGGIGTVPVGRVETGYPKAWYCCRFCPC*HSLLKSSSVGKCTTRLYKKLYPVTNV 185
+DVYKIGGIGTVPVGRVETG K F P + SV L L P NV
Sbjct: 249 QDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPA-GVTTEVKSVEMHHESLDAGL-PGDNV 306
Query: 186 GFNVKNVSVKEFAPWLRCRRFEKQPTQG 269
GFNVKNVSVK+ C + P G
Sbjct: 307 GFNVKNVSVKDIRRGNVCGDSKNDPPMG 334
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 153 bits (372), Expect = 1e-38
Identities = 75/145 (51%), Positives = 98/145 (67%)
Frame = +1
Query: 43 PWVELKLGILKPGTVVVFAPANIPY*SQVPWENAPRGSTRSCTR*QMLVSTSKTYLSRNL 222
P ++ G++KPG +V FAPA + ++V S + + K +++
Sbjct: 261 PVGRVETGVIKPGMIVTFAPAGVT--TEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDI 318
Query: 223 RRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVD 402
RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+D
Sbjct: 319 RRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKID 378
Query: 403 RRTGKSTEDNPKSIKSGDADIVNLV 477
RR+GK E++PK +KSGDA I +V
Sbjct: 379 RRSGKKIEESPKFVKSGDACIAKMV 403
Score = 63.7 bits (148), Expect = 1e-11
Identities = 42/88 (47%), Positives = 46/88 (52%)
Frame = +3
Query: 6 RDVYKIGGIGTVPVGRVETGYPKAWYCCRFCPC*HSLLKSSSVGKCTTRLYKKLYPVTNV 185
+DVYKIGGIGTVPVGRVETG K F P + SV L L P NV
Sbjct: 249 QDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPA-GVTTEVKSVEMHHESLDAGL-PGDNV 306
Query: 186 GFNVKNVSVKEFAPWLRCRRFEKQPTQG 269
GFNVKNVSVK+ C + P G
Sbjct: 307 GFNVKNVSVKDIRRGNVCGDSKNDPPMG 334
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 153 bits (372), Expect = 1e-38
Identities = 75/145 (51%), Positives = 98/145 (67%)
Frame = +1
Query: 43 PWVELKLGILKPGTVVVFAPANIPY*SQVPWENAPRGSTRSCTR*QMLVSTSKTYLSRNL 222
P ++ G++KPG +V FAPA + ++V S + + K +++
Sbjct: 261 PVGRVETGVIKPGMIVTFAPAGVT--TEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDI 318
Query: 223 RRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVD 402
RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+D
Sbjct: 319 RRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKID 378
Query: 403 RRTGKSTEDNPKSIKSGDADIVNLV 477
RR+GK E++PK +KSGDA I +V
Sbjct: 379 RRSGKKIEESPKFVKSGDACIAKMV 403
Score = 63.7 bits (148), Expect = 1e-11
Identities = 42/88 (47%), Positives = 46/88 (52%)
Frame = +3
Query: 6 RDVYKIGGIGTVPVGRVETGYPKAWYCCRFCPC*HSLLKSSSVGKCTTRLYKKLYPVTNV 185
+DVYKIGGIGTVPVGRVETG K F P + SV L L P NV
Sbjct: 249 QDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPA-GVTTEVKSVEMHHESLDAGL-PGDNV 306
Query: 186 GFNVKNVSVKEFAPWLRCRRFEKQPTQG 269
GFNVKNVSVK+ C + P G
Sbjct: 307 GFNVKNVSVKDIRRGNVCGDSKNDPPMG 334
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 45.2 bits (102), Expect = 5e-06
Identities = 23/68 (33%), Positives = 37/68 (54%)
Frame = +1
Query: 217 NLRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEK 396
+++ GYV +KN P F AQ+ +L P ++ GY+ V+ HTA FA++ K
Sbjct: 540 DVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHK 598
Query: 397 VDRRTGKS 420
+D+ KS
Sbjct: 599 LDKTNRKS 606
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 28.7 bits (61), Expect = 0.48
Identities = 13/25 (52%), Positives = 19/25 (76%)
Frame = -2
Query: 464 MSASPDLMDLGLSSVDLPVRRSTFS 390
+S +PDL D+ LSSVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 27.5 bits (58), Expect = 1.1
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +3
Query: 9 DVYKIGGIGTVPVGRVETG 65
DV+ I G GTV GRVE G
Sbjct: 259 DVFSISGRGTVVTGRVERG 277
>SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr
3|||Manual
Length = 396
Score = 26.6 bits (56), Expect = 1.9
Identities = 16/49 (32%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
Frame = -2
Query: 470 LTMSASPDLMDLGLSSVDL--PVRRSTFSLISANLQAMWAVWQSNTGVY 330
L S +L LG++ + P +RST S ++ L W + N GVY
Sbjct: 309 LGKSLVDELSKLGVTIIGSKDPKKRSTHSYVAKILNPEWDAFLKNEGVY 357
>SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 505
Score = 26.2 bits (55), Expect = 2.6
Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +3
Query: 264 QGSCRLHSASHCAKSPRSNIKRIHTCIGLPHSPHSLQIC-RNQRESRP 404
+GS ++S + +S + I CIG+ SPH +C R Q +P
Sbjct: 90 KGSTCAFTSSILQQIQKSGERSIPKCIGMYTSPHLRSVCERIQLNGKP 137
>SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 163
Score = 25.4 bits (53), Expect = 4.5
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -1
Query: 408 TTVDFLFDFGKFAGYVGCVAIQYRCVSV*YLTWV 307
T +D+LF F+ +G + Y ++V Y+ WV
Sbjct: 73 TLIDYLFFSPPFSLSIGPSLLVYLSIAVSYMLWV 106
>SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 474
Score = 24.6 bits (51), Expect = 7.9
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = +2
Query: 206 ICQGICAVVTLQEIRKTTHPGELQTSQRKSL 298
IC GIC +T +EI K+ +P E + + L
Sbjct: 347 ICSGICDGLTYEEI-KSIYPKEYEARKLDKL 376
>SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 462
Score = 24.6 bits (51), Expect = 7.9
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -2
Query: 389 LISANLQAMWAVWQSNTGV 333
L+SA+ W +W +TGV
Sbjct: 280 LVSASFDTTWRLWDVHTGV 298
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,180,510
Number of Sequences: 5004
Number of extensions: 46743
Number of successful extensions: 140
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 131
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 184476110
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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