Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0012_G19
(465 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 107 8e-25
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 85 7e-18
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 72 5e-14
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 68 8e-13
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 59 4e-10
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 56 3e-09
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 47 1e-06
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 37 0.001
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 36 0.003
SPAC1002.11 |gaa1||GPI-anchor transamidase complex subunit Gaa1 ... 30 0.15
SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr 2|||Ma... 28 0.61
SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint compone... 27 1.4
SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces ... 26 3.3
SPAC823.10c |||mitochondrial carrier with solute carrier repeats... 26 3.3
SPBC8D2.19 |mde3||serine/threonine protein kinase Mde3|Schizosac... 25 5.7
SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|ch... 25 5.7
SPCC330.08 |alg11|gmd3|alpha-1,2-mannosyltransferase Alg11|Schiz... 25 7.5
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 24 9.9
SPBC29A10.08 |||1,3-beta-glucanosyltransferase |Schizosaccharomy... 24 9.9
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 24 9.9
SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog Chs2|Schiz... 24 9.9
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 107 bits (257), Expect = 8e-25
Identities = 53/131 (40%), Positives = 80/131 (61%), Gaps = 1/131 (0%)
Frame = +3
Query: 36 VAIFLAITLLGFTLGDEIPTEDNVLVLSKSNFDSVVSTTNYILVEFYAPWCGHCKSLAPE 215
+A FLA + F E+P ++K + +++ ++V+FYAPWCGHCK+LAPE
Sbjct: 7 LAAFLAFSGGFFCASAEVPK------VNKEGLNELITADKVLMVKFYAPWCGHCKALAPE 60
Query: 216 YAKAATKLAEEESPIKLAKVDATQEQELAESYGVRGYPTLKFFRNGNPV-DYSGGRQADD 392
Y AA +L E+ I L +VD T+E +L Y +RGYPTL F+NG + YSG R+ D
Sbjct: 61 YESAADEL--EKDGISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDA 118
Query: 393 IVNWLKKKTGP 425
+V +++K+ P
Sbjct: 119 LVKYMRKQLLP 129
Score = 93.1 bits (221), Expect = 2e-20
Identities = 47/111 (42%), Positives = 73/111 (65%), Gaps = 4/111 (3%)
Frame = +3
Query: 93 TEDNVLVLSKSNFDSVV-STTNYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLA 269
++++++VL NFD +V T +LVEFYAPWCGHCK+LAP Y K A + + ++S + +A
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYS-DDSNVVVA 411
Query: 270 KVDATQEQELAESYGVRGYPTLKFFRNG---NPVDYSGGRQADDIVNWLKK 413
K+DAT E ++ S + G+PT+ FF+ NPV Y G R +D+ ++ K
Sbjct: 412 KIDAT-ENDI--SVSISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDK 459
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 84.6 bits (200), Expect = 7e-18
Identities = 37/89 (41%), Positives = 54/89 (60%), Gaps = 2/89 (2%)
Frame = +3
Query: 162 LVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQELAESYGVRGYPTLKF 341
L+EFYA WCGHCKSLAP Y + L E+ + + + K+DA ++A+ Y + G+PTL +
Sbjct: 43 LIEFYATWCGHCKSLAPVYEELGA-LFEDHNDVLIGKIDADTHSDVADKYHITGFPTLIW 101
Query: 342 F--RNGNPVDYSGGRQADDIVNWLKKKTG 422
F PV YS R D + ++ +KTG
Sbjct: 102 FPPDGSEPVQYSNARDVDSLTQFVSEKTG 130
Score = 80.2 bits (189), Expect = 1e-16
Identities = 41/117 (35%), Positives = 65/117 (55%), Gaps = 4/117 (3%)
Frame = +3
Query: 84 EIPTEDNVLVLSKSNFDSVVSTTNY-ILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPI 260
+I NV+ L NFD VV +LVEFYA WCG+CK LAP Y + K+ + E +
Sbjct: 135 KIVLPSNVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY-ETLGKVFKNEPNV 193
Query: 261 KLAKVDATQEQELAESYGVRGYPTLKFFRNGN---PVDYSGGRQADDIVNWLKKKTG 422
++ K++A ++ + V +PT+KFF + P Y G R + ++ ++ KK+G
Sbjct: 194 EIVKINADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKSG 250
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 71.7 bits (168), Expect = 5e-14
Identities = 45/144 (31%), Positives = 68/144 (47%), Gaps = 7/144 (4%)
Frame = +3
Query: 42 IFLA-ITLLGFTLGDEIPTEDNVLVLSKSNFDSVVSTTNYILVEFYAPWCGHCKSLAPEY 218
+FLA +L+ G N + L+ NF V LV FYAPWCG+CK L P Y
Sbjct: 11 LFLACFSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTY 70
Query: 219 AKAATKLAEEESPIKLAKVDATQEQELAESYGVRGYPTLKFF---RNGNPV---DYSGGR 380
K A+ L P+ DA Q + + Y V+G+PT+K G+ + DY+G R
Sbjct: 71 QKLASNL-HSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDR 129
Query: 381 QADDIVNWLKKKTGPPAVEVTSAE 452
+ ++ P V++ ++E
Sbjct: 130 SYKSLQKFVSDSI-PSKVKILTSE 152
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 67.7 bits (158), Expect = 8e-13
Identities = 37/121 (30%), Positives = 65/121 (53%), Gaps = 4/121 (3%)
Frame = +3
Query: 111 VLSKSNFDSVVSTTNYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 290
+ S ++ S + + Y+ V+ YA WCG CK+++P +++ A+K A + AKV+ ++
Sbjct: 6 IRSYQHWISTIPKSGYLAVDCYADWCGPCKAISPLFSQLASKYASPK--FVFAKVNVDEQ 63
Query: 291 QELAESYGVRGYPTLKFFRNGNPVDYSGGR--QA--DDIVNWLKKKTGPPAVEVTSAEQA 458
+++A GV+ PT FF NG +D G QA + + K TG A+ +S+
Sbjct: 64 RQIASGLGVKAMPTFVFFENGKQIDMLTGANPQALKEKVALISSKATGTGALASSSSAPV 123
Query: 459 K 461
K
Sbjct: 124 K 124
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 58.8 bits (136), Expect = 4e-10
Identities = 27/85 (31%), Positives = 44/85 (51%)
Frame = +3
Query: 111 VLSKSNFDSVVSTTNYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 290
V S F S+V ++V+F+A WCG CK++AP++ + + S KVD Q
Sbjct: 5 VSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF----EQFSNTYSDATFIKVDVDQL 60
Query: 291 QELAESYGVRGYPTLKFFRNGNPVD 365
E+A GV P+ ++NG ++
Sbjct: 61 SEIAAEAGVHAMPSFFLYKNGEKIE 85
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 56.0 bits (129), Expect = 3e-09
Identities = 31/101 (30%), Positives = 49/101 (48%)
Frame = +3
Query: 111 VLSKSNFDSVVSTTNYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 290
V S ++++ +S +V+FYA WCG CK L P KL+E+ V+A +
Sbjct: 22 VESFGDYNTRISADKVTVVDFYADWCGPCKYLKP----FLEKLSEQNQKASFIAVNADKF 77
Query: 291 QELAESYGVRGYPTLKFFRNGNPVDYSGGRQADDIVNWLKK 413
++A+ GV PT+ FR G +D G + + L K
Sbjct: 78 SDIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLSSLLAK 118
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 47.2 bits (107), Expect = 1e-06
Identities = 35/114 (30%), Positives = 54/114 (47%), Gaps = 2/114 (1%)
Frame = +3
Query: 30 MRVAIFLAITLLGFTLGDEIPTEDNVLVLSKSNFDSVVSTTNYILVEFYAPWCGHCKSLA 209
MR++ +A + TL +P DN L +S VS + +++Y P CG CK L
Sbjct: 10 MRISWVVAFITMVQTLVSGVPLTDNDL-------ESEVSKGTWF-IKYYLPSCGACKRLG 61
Query: 210 PEYAKAATKLAE--EESPIKLAKVDATQEQELAESYGVRGYPTLKFFRNGNPVD 365
P + K E E S +VD + +EL+ +R PTL ++NG V+
Sbjct: 62 PMWDNMVEKAKEQVEGSNFHFGEVDCS--KELSSCANIRAVPTLYLYQNGEIVE 113
Score = 39.1 bits (87), Expect = 3e-04
Identities = 19/108 (17%), Positives = 50/108 (46%)
Frame = +3
Query: 90 PTEDNVLVLSKSNFDSVVSTTNYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLA 269
PT + + ++ D+ ++ ++FY+ C C ++ + A ++ + +A
Sbjct: 278 PTGTSKALALDADIDAALTDKEGWFIQFYSSECDDCDDVSTAWYAMANRM---RGKLNVA 334
Query: 270 KVDATQEQELAESYGVRGYPTLKFFRNGNPVDYSGGRQADDIVNWLKK 413
++ + + Y ++ +PT FF+ V+Y G D+V++ ++
Sbjct: 335 HINCAVSKRACKQYSIQYFPTFLFFKEEAFVEYVGLPNEGDLVSFAEE 382
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 37.1 bits (82), Expect = 0.001
Identities = 14/87 (16%), Positives = 44/87 (50%)
Frame = +3
Query: 192 HCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQELAESYGVRGYPTLKFFRNGNPVDYS 371
HC+ A ++ + +K+A+V+ +E+E+ + ++ +PT + F+ + + Y+
Sbjct: 208 HCEDCFHWEAVWSSITRNTDERLKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYN 267
Query: 372 GGRQADDIVNWLKKKTGPPAVEVTSAE 452
G + ++++ + A+++ +
Sbjct: 268 GPLKYQQLLSYSNQVASYQAIKIEEGD 294
Score = 27.9 bits (59), Expect = 0.81
Identities = 17/54 (31%), Positives = 25/54 (46%), Gaps = 5/54 (9%)
Frame = +3
Query: 297 LAESYGVRGYPTLKFFRNGNPVDYSG--GRQADD---IVNWLKKKTGPPAVEVT 443
LA YG + P++ RNG P+ Y R+ D I W+ + P E+T
Sbjct: 343 LANKYGAQSQPSIIAVRNGMPIVYQAITPREFRDYKRITEWINIVSSPFITELT 396
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 35.9 bits (79), Expect = 0.003
Identities = 17/57 (29%), Positives = 29/57 (50%)
Frame = +3
Query: 159 ILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQELAESYGVRGYP 329
IL+ FYAPW CK + + + A++ K++A + ++AES+ V P
Sbjct: 23 ILLNFYAPWAAPCKQMNQVF----DQFAKDTKNAVFLKIEAEKFSDIAESFDVNAVP 75
>SPAC1002.11 |gaa1||GPI-anchor transamidase complex subunit Gaa1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 581
Score = 30.3 bits (65), Expect = 0.15
Identities = 20/52 (38%), Positives = 29/52 (55%), Gaps = 5/52 (9%)
Frame = +3
Query: 24 IKMRVAIFLAITLLGFTLGDEIPTEDNVLVLS-----KSNFDSVVSTTNYIL 164
I + + ++L +T +G + +ED LVLS KS F SVVST N+ L
Sbjct: 428 ITVFIFLYLMLTFIGIIFSTFMTSEDAELVLSYDLMSKSLFISVVSTLNFSL 479
>SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 318
Score = 28.3 bits (60), Expect = 0.61
Identities = 17/43 (39%), Positives = 23/43 (53%)
Frame = +3
Query: 192 HCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQELAESYGVR 320
+C L P +AA L + +SP L VDA + L S+GVR
Sbjct: 183 YCAYLVPNEHEAAILLNQADSPATLENVDAYASKLL--SFGVR 223
>SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint component
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 809
Score = 27.1 bits (57), Expect = 1.4
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +1
Query: 223 RLPLSWQRKNLR*NWRKLMPLRNKSLPKVTELEDT 327
R P S + N+ +W K + L K K+TE++ T
Sbjct: 766 RSPFSSRANNINPDWTKAIDLAAKLKQKITEMKQT 800
>SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1098
Score = 25.8 bits (54), Expect = 3.3
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +3
Query: 348 NGNPVDYSGGRQADDIVNWLKKKT 419
N N +D G +Q DD+V+ ++K T
Sbjct: 807 NSNVMDEQGEKQKDDVVDMVRKIT 830
>SPAC823.10c |||mitochondrial carrier with solute carrier
repeats|Schizosaccharomyces pombe|chr 1|||Manual
Length = 296
Score = 25.8 bits (54), Expect = 3.3
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = -3
Query: 151 VVDTTLSKLDLLRTRTLSSVGISSPRVNPRRVI 53
+ TTL LDLL+TR S S P++ RR+I
Sbjct: 27 ISSTTLQPLDLLKTRCQQSQRDSLPKM-VRRII 58
>SPBC8D2.19 |mde3||serine/threonine protein kinase
Mde3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 559
Score = 25.0 bits (52), Expect = 5.7
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +3
Query: 48 LAITLLGFTLGDEIPTEDNVLVLSKSNF 131
+AI T+GD IP+ ++L+ K F
Sbjct: 459 IAINAFNETVGDRIPSSKDILITEKIPF 486
>SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 778
Score = 25.0 bits (52), Expect = 5.7
Identities = 9/13 (69%), Positives = 10/13 (76%)
Frame = -2
Query: 56 YSQKYCYTHLDDP 18
YS+K Y HLDDP
Sbjct: 61 YSEKILYGHLDDP 73
>SPCC330.08 |alg11|gmd3|alpha-1,2-mannosyltransferase
Alg11|Schizosaccharomyces pombe|chr 3|||Manual
Length = 471
Score = 24.6 bits (51), Expect = 7.5
Identities = 11/33 (33%), Positives = 19/33 (57%)
Frame = +3
Query: 360 VDYSGGRQADDIVNWLKKKTGPPAVEVTSAEQA 458
V+ SGG + D ++ W+ K TG A ++ +A
Sbjct: 389 VNNSGGPKFDIVIPWIGKPTGFHASTISEYAEA 421
>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1016
Score = 24.2 bits (50), Expect = 9.9
Identities = 10/23 (43%), Positives = 11/23 (47%)
Frame = +3
Query: 177 APWCGHCKSLAPEYAKAATKLAE 245
A WC HC + P K A K E
Sbjct: 484 ANWCCHCGYILPLGRKTARKCTE 506
>SPBC29A10.08 |||1,3-beta-glucanosyltransferase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 459
Score = 24.2 bits (50), Expect = 9.9
Identities = 13/43 (30%), Positives = 19/43 (44%)
Frame = +3
Query: 144 STTNYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAK 272
S T+++ Y WCGH YA +L PI L++
Sbjct: 222 SETDFLGYNMYE-WCGHSDFYTSGYAARTQELENFTIPIFLSE 263
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 24.2 bits (50), Expect = 9.9
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = +1
Query: 190 VTANLLRQNTLRLPLSW 240
V+A LL TL+LPL+W
Sbjct: 446 VSAELLLLGTLQLPLAW 462
>SPBC1709.01 |chs2|SPBC1734.17|chitin synthase homolog
Chs2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 926
Score = 24.2 bits (50), Expect = 9.9
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = +3
Query: 105 VLVLSKSNFDSVVSTTNYILVEFYAPW 185
+L KS +S S Y++V+F++ W
Sbjct: 556 ILESKKSEINSSFSLAVYVIVDFFSLW 582
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,827,717
Number of Sequences: 5004
Number of extensions: 34500
Number of successful extensions: 151
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 176367270
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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