Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0012_G14
(529 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC732.02c |||fructose-2,6-bisphosphate 2-phosphatase activity ... 181 5e-47
SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces ... 128 4e-31
SPAC222.13c |||6-phosphofructo-2-kinase |Schizosaccharomyces pom... 87 1e-18
SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb... 40 2e-04
SPBC3B8.02 |php5||CCAAT-binding factor complex subunit Php5|Schi... 26 3.0
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 26 4.0
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 25 5.3
SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch... 25 9.2
SPAC22F3.03c |rdh54|tid1, mug34|ATP-dependent DNA helicase Rdh54... 25 9.2
SPAP7G5.02c |gua2||GMP synthase [glutamine-hydrolyzing] |Schizos... 25 9.2
>SPAC732.02c |||fructose-2,6-bisphosphate 2-phosphatase activity
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 408
Score = 181 bits (441), Expect = 5e-47
Identities = 84/174 (48%), Positives = 118/174 (67%), Gaps = 1/174 (0%)
Frame = +1
Query: 10 HGESEYNVLGRIGGDAPLSARGQLYARALADHFNGLCTHEP-LSVWTSELRRTKQTAADV 186
HGES++NV G+IGGD+ LS +G YA L ++ E L+VWTS + RT QTA +
Sbjct: 214 HGESQFNVEGKIGGDSSLSPQGLKYAALLPEYVAKFSIGEKGLTVWTSSMARTIQTARHL 273
Query: 187 RAPKRAIRALNELDAGICEGLTYEEMQERFPQEFAWRDQDKLRYRYPWGESYIDIMTRLR 366
K RAL+ELDAG C+G TY+ +++ FP E R+ DK YRY GESY+D++ RL
Sbjct: 274 NCQKLEWRALDELDAGTCDGFTYDYIEQNFPHEAELRNNDKFHYRYRGGESYMDVVRRLE 333
Query: 367 PVLSALEDEHNVIVVGHQAVLRCMLGYFLDAKLDELPYMNVPLHTIVKLTSYGY 528
P++ LE + +V ++ HQA+LRC+ GY+ + L+ELP++NVPLHTI+KLT Y
Sbjct: 334 PIIMELERQGDVFIICHQAILRCIYGYYHNLSLEELPFINVPLHTIIKLTPMTY 387
>SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 474
Score = 128 bits (310), Expect = 4e-31
Identities = 60/132 (45%), Positives = 96/132 (72%), Gaps = 6/132 (4%)
Frame = +1
Query: 127 EPLSVWTSELRRTKQTAA---DVRAPKRAIRALNELDAGICEGLTYEEMQERFPQEFAWR 297
+P SVWTS ++R+ QTAA + + +A+R LNE+ +GIC+GLTYEE++ +P+E+ R
Sbjct: 312 KPYSVWTSMMQRSIQTAAYFDEEQYDIKAMRMLNEICSGICDGLTYEEIKSIYPKEYEAR 371
Query: 298 DQDKLRYRYP--WGESYIDIMTRLRPVLSALED-EHNVIVVGHQAVLRCMLGYFLDAKLD 468
DKL YRYP GESY+D++ RL+ V+ +E +H+V+V+GH+ + R ++ YFL + +
Sbjct: 372 KLDKLNYRYPGSGGESYLDVIYRLQSVIVEIERMKHHVLVIGHRVITRIIIAYFLGCRRE 431
Query: 469 ELPYMNVPLHTI 504
++ Y+NVPLHT+
Sbjct: 432 DIAYLNVPLHTV 443
Score = 33.9 bits (74), Expect = 0.015
Identities = 16/30 (53%), Positives = 21/30 (70%)
Frame = +1
Query: 10 HGESEYNVLGRIGGDAPLSARGQLYARALA 99
HGES NV GRIGG+A L+ G+ ++ LA
Sbjct: 216 HGESVDNVRGRIGGNAELTPLGRQFSEDLA 245
>SPAC222.13c |||6-phosphofructo-2-kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 592
Score = 87.4 bits (207), Expect = 1e-18
Identities = 63/199 (31%), Positives = 99/199 (49%), Gaps = 27/199 (13%)
Frame = +1
Query: 13 GESEYNVLGRIGGDAPLSARGQLYARALADHFN---------------------GLCTHE 129
GES V GRIGGDA L+ G+ YA+ LA+ + L
Sbjct: 366 GESLDTVAGRIGGDASLTPIGKQYAQDLANFMDRQRVLWQLRYTNDLASTNKRFSLSEAS 425
Query: 130 PLSVWTSELRRTKQTAADVRAPK---RAIRALNELDAGICEGLTYEEMQERFPQEFAWRD 300
+VW+S +R +T + IR LN+L+ G EGLT E E++P EF
Sbjct: 426 SFNVWSSVRKRAIETIEFFNPDSYNVKKIRLLNDLNLGSREGLTLREFSEKYPDEFDVIK 485
Query: 301 QDKLRYRY--PWGESYIDIMTRLRPVLSALE-DEHNVIVVGHQAVLRCMLGYFLDAKLDE 471
+ YR+ GESY+D++ RL+P++ +E N++VV H+ V ++ YFL+ ++
Sbjct: 486 RKDYAYRFSGQGGESYLDVIHRLQPLIVEIERSSGNILVVSHRIVSNILMTYFLNYHPED 545
Query: 472 LPYMNVPLHTIVKLTSYGY 528
+ + +PLHT+ + S Y
Sbjct: 546 IIDVGLPLHTLFCIESDRY 564
>SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 432
Score = 40.3 bits (90), Expect = 2e-04
Identities = 16/52 (30%), Positives = 33/52 (63%), Gaps = 1/52 (1%)
Frame = +1
Query: 304 DKLRYRYPWGESYIDIMTRLRPVLSAL-EDEHNVIVVGHQAVLRCMLGYFLD 456
D RYR ES+ D+ RL P++ L ++ +V+++G ++++R GY+++
Sbjct: 365 DPYRYRVKRKESFYDLAVRLEPLILELGREQRDVLLIGSKSIIRVFYGYYMN 416
>SPBC3B8.02 |php5||CCAAT-binding factor complex subunit
Php5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 415
Score = 26.2 bits (55), Expect = 3.0
Identities = 12/40 (30%), Positives = 21/40 (52%)
Frame = -1
Query: 394 VRLQAPTAPAEAASLCLYRIPPTGIDNGACPDHAKRTPGG 275
+ +Q P A + LYR PPT +++G+ + +P G
Sbjct: 294 MNMQRPVMVAPYMAEHLYRYPPTHLESGSSAFRLQSSPMG 333
>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 640
Score = 25.8 bits (54), Expect = 4.0
Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 2/51 (3%)
Frame = -3
Query: 161 LLSSDVHTDSGSCVHSPLKWSASARAYS*PLAERGASPPI--RPSTLYSLS 15
L++ ++HT + SP+ +AS+ + P+ S PI RP ++ +LS
Sbjct: 288 LIAQNLHTSASQVSLSPMASTASSSVTNSPVDTHTPSTPIMSRPPSMKALS 338
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 25.4 bits (53), Expect = 5.3
Identities = 11/37 (29%), Positives = 20/37 (54%)
Frame = -2
Query: 324 VSITELVLITPSELLGETFLHLLIRQTFTDASIEFIE 214
+++ E P E+L + LL +TF + IEF++
Sbjct: 887 LALVEQAYDNPHEMLSQIKRRLLTMRTFKEVGIEFMD 923
>SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr
3|||Manual
Length = 923
Score = 24.6 bits (51), Expect = 9.2
Identities = 18/78 (23%), Positives = 36/78 (46%), Gaps = 2/78 (2%)
Frame = +1
Query: 199 RAIRALNELDAGICEGLTYEEMQERFPQ-EFAWRDQDKLRYRYPWGESYIDIMTRL-RPV 372
+ + A+ I E L E +Q +F + ++ Y Y +G SY D +RL + +
Sbjct: 280 QTLEAMGNKPFWIEEKLDGERIQLHMSSGKFQFYSRNARSYTYAYGSSYFDEQSRLTQYI 339
Query: 373 LSALEDEHNVIVVGHQAV 426
+ A + + I++ + V
Sbjct: 340 IGAFDKRISQIILDGEMV 357
>SPAC22F3.03c |rdh54|tid1, mug34|ATP-dependent DNA helicase
Rdh54|Schizosaccharomyces pombe|chr 1|||Manual
Length = 811
Score = 24.6 bits (51), Expect = 9.2
Identities = 10/25 (40%), Positives = 17/25 (68%), Gaps = 1/25 (4%)
Frame = -3
Query: 74 PLAERGASPPIRPSTLY-SLSPCPR 3
P +R PP+RP+T+Y ++S P+
Sbjct: 144 PEMKRKFKPPLRPNTMYQTISALPK 168
>SPAP7G5.02c |gua2||GMP synthase [glutamine-hydrolyzing]
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 539
Score = 24.6 bits (51), Expect = 9.2
Identities = 15/37 (40%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
Frame = -2
Query: 309 LVLITPSELLGETF-LHLLIRQTFTDASIEFIERPDG 202
L+ + +E++ ET HL I+ T DAS EFI + G
Sbjct: 273 LLRLNEAEIVRETLNKHLGIQLTVVDASEEFIGKLKG 309
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,209,005
Number of Sequences: 5004
Number of extensions: 44306
Number of successful extensions: 123
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 117
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 216376042
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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