Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0011_F12
(569 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 255 3e-69
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 255 3e-69
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 255 3e-69
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 83 3e-17
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 50 2e-07
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 37 0.002
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 27 1.5
SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 27 2.6
SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces p... 26 3.4
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 26 3.4
SPAC15E1.05c |||ethanolamine-phosphate cytidylyltransferase |Sch... 26 3.4
SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr 3|||Ma... 26 3.4
SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces pom... 25 5.9
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 25 5.9
SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam... 25 7.8
SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 25 7.8
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 255 bits (625), Expect = 3e-69
Identities = 114/150 (76%), Positives = 132/150 (88%)
Frame = +3
Query: 9 VEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPG 188
VEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPG
Sbjct: 290 VEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPG 349
Query: 189 QISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLC 368
QIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E++PK +KSGDA I +VPSKP+C
Sbjct: 350 QISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMC 409
Query: 369 VESFQEFPPLGRFAVRDMRQTVAVGVIKAV 458
VE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 410 VEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 255 bits (625), Expect = 3e-69
Identities = 114/150 (76%), Positives = 132/150 (88%)
Frame = +3
Query: 9 VEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPG 188
VEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPG
Sbjct: 290 VEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPG 349
Query: 189 QISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLC 368
QIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E++PK +KSGDA I +VPSKP+C
Sbjct: 350 QISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMC 409
Query: 369 VESFQEFPPLGRFAVRDMRQTVAVGVIKAV 458
VE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 410 VEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 255 bits (625), Expect = 3e-69
Identities = 114/150 (76%), Positives = 132/150 (88%)
Frame = +3
Query: 9 VEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPG 188
VEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPG
Sbjct: 290 VEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPG 349
Query: 189 QISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLC 368
QIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK E++PK +KSGDA I +VPSKP+C
Sbjct: 350 QISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMC 409
Query: 369 VESFQEFPPLGRFAVRDMRQTVAVGVIKAV 458
VE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 410 VEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 83.0 bits (196), Expect = 3e-17
Identities = 47/147 (31%), Positives = 78/147 (53%), Gaps = 1/147 (0%)
Frame = +3
Query: 24 EALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNG 203
E + ++ GD V V+ +++ GYV +KN P F AQ+ +L P ++ G
Sbjct: 519 EEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTG 576
Query: 204 YTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQ 383
Y+ V+ HTA FA++ K+D+ T + ++ P G I L P+C+E F+
Sbjct: 577 YSCVMHIHTAVEEVSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFE 635
Query: 384 EFPPLGRFAVRDMRQTVAVG-VIKAVN 461
++ +GRF +RD TVAVG V+K ++
Sbjct: 636 DYQYMGRFTLRDQGTTVAVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 50.4 bits (115), Expect = 2e-07
Identities = 42/138 (30%), Positives = 63/138 (45%)
Frame = +3
Query: 39 AVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNHPGQISNGYTPVL 218
AV GD V + ++ V +LR G + + +N P R F A++ + G I +G T VL
Sbjct: 461 AVAGDTVTLQLADIEVNQLRPGDILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL 519
Query: 219 DCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 398
H+ + K+ K + + S K I L PLC+ +E P L
Sbjct: 520 -----HLGRTVTSVSLKIVTVNNKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPAL 571
Query: 399 GRFAVRDMRQTVAVGVIK 452
GRF +R TVA G++K
Sbjct: 572 GRFILRRSGDTVAAGIVK 589
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 37.1 bits (82), Expect = 0.002
Identities = 33/149 (22%), Positives = 62/149 (41%)
Frame = +3
Query: 3 TRVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPRGAADFTAQVIVLNH 182
T +EM + L AV GDN G ++++ ++L+RG + P F A +L
Sbjct: 299 TGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMIVAQPGTVAPH--QKFKASFYILTK 356
Query: 183 PGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEDNPKSIKSGDAAIVNLVPSKP 362
+ T +D + + + +++ ++ T D+ K + GD + P
Sbjct: 357 --EEGGRRTGFVDKYRPQLYSRTSDVTVEL---THPDPNDSDKMVMPGDNVEMICTLIHP 411
Query: 363 LCVESFQEFPPLGRFAVRDMRQTVAVGVI 449
+ +E Q RF VR+ TV ++
Sbjct: 412 IVIEKGQ------RFTVREGGSTVGTALV 434
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 27.5 bits (58), Expect = 1.5
Identities = 12/25 (48%), Positives = 19/25 (76%)
Frame = -2
Query: 337 MAASPDLMDLGLSSVDLPVRRSTFS 263
++ +PDL D+ LSSVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
>SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1190
Score = 26.6 bits (56), Expect = 2.6
Identities = 19/71 (26%), Positives = 34/71 (47%), Gaps = 3/71 (4%)
Frame = +1
Query: 310 NPLNLVMPPLSTWFPPSPCVWS---PSRNSHPSVVSPCVT*GRRSPSV**RL*TSRKQVV 480
+PL+ + P L WF V+S P+R+S + P + + SP+ +++V+
Sbjct: 707 SPLSELHPQLREWFSSKQSVYSLQYPTRDSIIAFFQPILELIKASPTELPGGIPRKRRVL 766
Query: 481 AKSPKLPKKPP 513
+ P P PP
Sbjct: 767 PELPLAPDPPP 777
>SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 505
Score = 26.2 bits (55), Expect = 3.4
Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +2
Query: 137 QGSCRLHSASHCAKSPRSNIKRIHTCIGLPHSPHSLQIC-RNQRESRP 277
+GS ++S + +S + I CIG+ SPH +C R Q +P
Sbjct: 90 KGSTCAFTSSILQQIQKSGERSIPKCIGMYTSPHLRSVCERIQLNGKP 137
>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 26.2 bits (55), Expect = 3.4
Identities = 14/44 (31%), Positives = 21/44 (47%)
Frame = +1
Query: 16 CTTRLYKKLYPVTMLVSTSKTYLSRNCAVVTLQEIRKTTHPGEL 147
CT + KL+PV L T + A+ T+ I T PG++
Sbjct: 196 CTMSIEGKLFPVETLFLQKPTENYVDSAIETVININSTYPPGDI 239
>SPAC15E1.05c |||ethanolamine-phosphate cytidylyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 365
Score = 26.2 bits (55), Expect = 3.4
Identities = 15/54 (27%), Positives = 28/54 (51%)
Frame = -2
Query: 358 LEGTRLTMAASPDLMDLGLSSVDLPVRRSTFSLISANLQAMWAVWQSNTGVYPF 197
LE R ++ +L+D LSSV L + + S++S+ + + + G+ PF
Sbjct: 122 LEVKRTEGVSTTELLDRLLSSVPLEIYSTPVSVLSSQIDLLRRFATDSDGLTPF 175
>SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr
3|||Manual
Length = 396
Score = 26.2 bits (55), Expect = 3.4
Identities = 14/42 (33%), Positives = 22/42 (52%), Gaps = 2/42 (4%)
Frame = -2
Query: 322 DLMDLGLSSVDL--PVRRSTFSLISANLQAMWAVWQSNTGVY 203
+L LG++ + P +RST S ++ L W + N GVY
Sbjct: 316 ELSKLGVTIIGSKDPKKRSTHSYVAKILNPEWDAFLKNEGVY 357
>SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 163
Score = 25.4 bits (53), Expect = 5.9
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -1
Query: 281 TTVDFLFDFGKFAGYVGCVAIQYRCVSV*YLTWV 180
T +D+LF F+ +G + Y ++V Y+ WV
Sbjct: 73 TLIDYLFFSPPFSLSIGPSLLVYLSIAVSYMLWV 106
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.4 bits (53), Expect = 5.9
Identities = 24/85 (28%), Positives = 43/85 (50%), Gaps = 2/85 (2%)
Frame = -2
Query: 502 SAALVTLPPPASLKFTAFITPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTRLTMAA 329
S +VTLPPPAS ++F T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 328 SPDLMDLGLSSVDLPVRRSTFSLIS 254
S L ++S PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein
Mam3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1082
Score = 25.0 bits (52), Expect = 7.8
Identities = 15/68 (22%), Positives = 33/68 (48%)
Frame = +1
Query: 46 PVTMLVSTSKTYLSRNCAVVTLQEIRKTTHPGELQTSQRKSLC*ITQVKYQTDTHLYWIA 225
P + +++ S + S + +VT ++ TTH +++T + T + D+H +
Sbjct: 379 PTSSILTNSGSIKSGDHQIVTTSFVQTTTHGSQVETLTYVTTLTETILTTTYDSHTFLTT 438
Query: 226 TQPT*PAN 249
P+ P+N
Sbjct: 439 ITPS-PSN 445
>SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 25.0 bits (52), Expect = 7.8
Identities = 10/37 (27%), Positives = 19/37 (51%)
Frame = -2
Query: 517 PLVAFSAALVTLPPPASLKFTAFITPTATVCLMSRTA 407
P + ++ T+PP S+ T + PT ++ + TA
Sbjct: 73 PSTSHNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTA 109
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,550,996
Number of Sequences: 5004
Number of extensions: 55574
Number of successful extensions: 149
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 242064240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -