Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0011_A21
(478 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC191.09c |gst1||glutathione S-transferase Gst1|Schizosaccharo... 46 3e-06
SPCC965.07c |gst2||glutathione S-transferase Gst2|Schizosaccharo... 43 3e-05
SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce... 42 6e-05
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 30 0.21
SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc... 28 0.84
SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces pomb... 27 1.1
SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3 |Schizosacchar... 26 2.6
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 26 2.6
SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomy... 26 3.4
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 4.5
SPAC2F3.01 ||SPAC323.09|mannosyltransferase complex subunit |Sch... 25 4.5
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 5.9
SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyce... 25 5.9
>SPCC191.09c |gst1||glutathione S-transferase
Gst1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 229
Score = 46.0 bits (104), Expect = 3e-06
Identities = 28/67 (41%), Positives = 35/67 (52%), Gaps = 4/67 (5%)
Frame = +2
Query: 164 EHLKPEFLKLNPQHTVPTLVD---DGLSIWESRAIITYLVNKYGKGSSL-YPEDARARAL 331
E PE L LNP VPTL+D + +IWES AI+ YL +KY + P D
Sbjct: 40 EQKSPEHLALNPNGRVPTLIDHHNNDYTIWESDAILIYLADKYDTERKISLPRDHPEYYK 99
Query: 332 VDQRLYF 352
V Q L+F
Sbjct: 100 VIQYLFF 106
>SPCC965.07c |gst2||glutathione S-transferase
Gst2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 230
Score = 42.7 bits (96), Expect = 3e-05
Identities = 23/44 (52%), Positives = 27/44 (61%), Gaps = 3/44 (6%)
Frame = +2
Query: 161 GEHLKPEFLKLNPQHTVPTLVD---DGLSIWESRAIITYLVNKY 283
GE E L LNP VPTLVD + +IWES AI+ YL +KY
Sbjct: 39 GEQKCKEHLALNPNGRVPTLVDHKNNDYTIWESDAILIYLADKY 82
>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 242
Score = 41.5 bits (93), Expect = 6e-05
Identities = 22/64 (34%), Positives = 33/64 (51%)
Frame = +2
Query: 176 PEFLKLNPQHTVPTLVDDGLSIWESRAIITYLVNKYGKGSSLYPEDARARALVDQRLYFD 355
P + KL+P P +VDDG++ ES AI+ +LV KYG ED + ++F
Sbjct: 40 PAYTKLSPLGKSPIVVDDGVTYIESAAILEHLVRKYGPSFKPSEEDVAELEKYELWMHFS 99
Query: 356 IGTL 367
+L
Sbjct: 100 EASL 103
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 29.9 bits (64), Expect = 0.21
Identities = 18/48 (37%), Positives = 28/48 (58%), Gaps = 3/48 (6%)
Frame = -1
Query: 463 KLESVLDLGQLLVVGRRSGKYLRIEV---ISESLVEGTNVKVQALIDE 329
KLE V+DL Q+ + R K +IE+ + E+L+E K ALI++
Sbjct: 241 KLEGVVDLIQMRAIYNRGSKGEKIEISQQVPENLIELAKEKRSALIEK 288
>SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 721
Score = 27.9 bits (59), Expect = 0.84
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = +2
Query: 227 DGLSIWESRAIITYLVNKYGKGSSLYPEDARARALVDQRLYF 352
+ LSI ESR I + ++G S L+P D + A + RL F
Sbjct: 263 NSLSI-ESRLTIANMTTEWGALSGLFPTDEKLLAWYEDRLKF 303
>SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 386
Score = 27.5 bits (58), Expect = 1.1
Identities = 19/71 (26%), Positives = 35/71 (49%), Gaps = 5/71 (7%)
Frame = +3
Query: 258 SSLTWSTSTVKAAASTQKTLERGRSSISACTLTLVPSTRDSEITSIRK-----YLPERLP 422
SS+ W++++ + + K SS+ + + +PS+ ++ +S+ K YLP L
Sbjct: 62 SSVKWASTSFDSTVTAHKEETPYSSSLGSHDSSSLPSSTNNRYSSVLKELCNTYLPSILS 121
Query: 423 TTRSWPRSRTL 455
T S P R L
Sbjct: 122 TYGSLPIRRLL 132
>SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 569
Score = 26.2 bits (55), Expect = 2.6
Identities = 11/32 (34%), Positives = 21/32 (65%)
Frame = +3
Query: 345 CTLTLVPSTRDSEITSIRKYLPERLPTTRSWP 440
CT+ +P+T++ + R ++P R PT ++WP
Sbjct: 139 CTM-YIPATKEHPL---RIWVPRRSPTKQTWP 166
>SPAC1834.01 |sup45||translation release factor
eRF1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 433
Score = 26.2 bits (55), Expect = 2.6
Identities = 8/26 (30%), Positives = 17/26 (65%)
Frame = +2
Query: 224 DDGLSIWESRAIITYLVNKYGKGSSL 301
+ + IW+ R ++ L+N +G G+S+
Sbjct: 6 EKAIEIWKIRRLVKQLINCHGNGTSM 31
>SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 648
Score = 25.8 bits (54), Expect = 3.4
Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 3/40 (7%)
Frame = +2
Query: 173 KPEFLKLNPQHTVPTLVDDGL---SIWESRAIITYLVNKY 283
KP+FL NPQ V + V +GL + E + YL+ +Y
Sbjct: 349 KPKFLDANPQSAVASRVLEGLIECAPAEKDLYLYYLIMRY 388
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 4.5
Identities = 24/77 (31%), Positives = 35/77 (45%)
Frame = +3
Query: 246 SRAPSSLTWSTSTVKAAASTQKTLERGRSSISACTLTLVPSTRDSEITSIRKYLPERLPT 425
S PS + S+ST +AA T T SS S + + PS+ S + +P+
Sbjct: 184 SSQPSVSSTSSSTFSSAAPTS-TSSSYLSSSSVVSSSSSPSSSSSSTLTSSSLSTSSIPS 242
Query: 426 TRSWPRSRTLSSFWTNS 476
T S S T SS ++S
Sbjct: 243 TSS-SSSSTSSSLSSSS 258
>SPAC2F3.01 ||SPAC323.09|mannosyltransferase complex subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 319
Score = 25.4 bits (53), Expect = 4.5
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -1
Query: 364 GTNVKVQALIDERPRSSVFWVEAAAFTVL 278
GT+ KV ID +S +F+ AAF +L
Sbjct: 263 GTDAKVFIWIDRNSKSVLFFAFLAAFAIL 291
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 25.0 bits (52), Expect = 5.9
Identities = 24/70 (34%), Positives = 32/70 (45%), Gaps = 4/70 (5%)
Frame = +3
Query: 255 PSSLTWSTSTVKAAASTQKTLERG----RSSISACTLTLVPSTRDSEITSIRKYLPERLP 422
P + T STST AST T +S S T T +P T +S T + +P P
Sbjct: 360 PPTSTSSTSTPPPPASTSSTGTSSSPLLSTSTSCTTSTSIPPTGNS-TTPVTPTVP---P 415
Query: 423 TTRSWPRSRT 452
T+ S P + T
Sbjct: 416 TSSSTPLTTT 425
>SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 204
Score = 25.0 bits (52), Expect = 5.9
Identities = 15/37 (40%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = +2
Query: 362 TLYQ-RFGDYFYPQVFAGAPADNEKLAKIEDALQLLD 469
TLY+ RFG YF V AG DN D++ +D
Sbjct: 94 TLYEKRFGPYFSFPVVAGVSNDNTPFICGFDSIGCID 130
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,766,834
Number of Sequences: 5004
Number of extensions: 30565
Number of successful extensions: 89
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 84
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 87
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 184476110
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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