Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0010_H16
(614 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 73 4e-14
SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po... 57 2e-09
SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 32 0.057
SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta... 32 0.076
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch... 31 0.13
SPCC18.13 |||tRNA |Schizosaccharomyces pombe|chr 3|||Manual 29 0.53
SPCC1223.11 |ptc2||protein phosphatase 2C Ptc2 |Schizosaccharomy... 26 3.8
SPBC146.04 |||sulfhydryl oxidase |Schizosaccharomyces pombe|chr ... 26 3.8
SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein k... 26 5.0
SPBC3E7.06c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 8.7
SPAC57A10.08c |||esterase/lipase |Schizosaccharomyces pombe|chr ... 25 8.7
>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 72.5 bits (170), Expect = 4e-14
Identities = 49/162 (30%), Positives = 84/162 (51%), Gaps = 4/162 (2%)
Frame = +2
Query: 137 KFPAGKVPAFESADGKVLLTESNAIAYYVANDD----LRGGDLATQARVLQWASWADSEL 304
KFP K+P F DG L+E+ AIA+Y+A+ + L G +A+VLQ+ S+ +SEL
Sbjct: 46 KFPLQKMPVFVGKDG-FPLSETLAIAFYLASLNKTRALNGTTAEEKAKVLQYCSFTNSEL 104
Query: 305 LPASCAWVFPYLGIMQFNKQNVERAKNXXXXXXXXXXXXXXSRTFLVTERITLADIIVYS 484
A + P + +++Q + A+ S+T+LV R+TLADI
Sbjct: 105 PGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTYLVGSRLTLADIFFTC 164
Query: 485 TLLHAFQHVLDPTVRSKLVNVQRWFLTVGHQPQVSSVVREPV 610
L +VL + +K ++ R++ T+ HQ ++ ++ EP+
Sbjct: 165 FLKFGATYVLTKSYLAKYTHIYRYYQTIYHQAKLDAIT-EPL 205
>SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 220
Score = 57.2 bits (132), Expect = 2e-09
Identities = 40/160 (25%), Positives = 77/160 (48%), Gaps = 8/160 (5%)
Frame = +2
Query: 137 KFPAGKVPAFESADGKVLLTESNAIAYYVA---NDDLRG-GDL--ATQARVLQWASWADS 298
KFP K+P F ADG L + Y+ ++D G G + +A +L+W + +
Sbjct: 46 KFPLQKLPVFIGADGFELSEVIAIVKYFYEKGKHNDKEGLGPVNEVEEAEMLKWMCFINF 105
Query: 299 ELL-PASCA-WVFPYLGIMQFNKQNVERAKNXXXXXXXXXXXXXXSRTFLVTERITLADI 472
+++ P + WV + G + + ++ + + RT+LV +R TLAD+
Sbjct: 106 DIVTPQNVRPWVGMFRGNIPYEEKPFKESATRAIDSLKIPNELVKDRTYLVGDRFTLADL 165
Query: 473 IVYSTLLHAFQHVLDPTVRSKLVNVQRWFLTVGHQPQVSS 592
S L F ++D R +L ++ R+++T+ HQ ++ +
Sbjct: 166 FFGSLLRIFFNSIIDEKTRKELPHLTRYYITMFHQAKLET 205
>SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase
Cdc25|Schizosaccharomyces pombe|chr 1|||Manual
Length = 596
Score = 32.3 bits (70), Expect = 0.057
Identities = 35/138 (25%), Positives = 63/138 (45%), Gaps = 10/138 (7%)
Frame = -3
Query: 429 LKRCPSRSCSAASKSFFARSTFCLLNCMMPKYGKTHAQDAGSSSLSAHEA-HCNTRACVA 253
LK R+ + FF +S + + ++ +GKT + + +SSL+A + + + A
Sbjct: 28 LKLMSDRNANQELDFFFPKSKH-IASTLVDPFGKTCSTASPASSLAADMSMNMHIDESPA 86
Query: 252 RSPPRRSSFAT**A-----IALLSVSKTLPSALSNAGTLPAGNFFK----KFSVLLVSPN 100
PRR+ F + +A ++ LP + SN L FF+ K+S+ SP
Sbjct: 87 LPTPRRTLFRSLSCTVETPLANKTIVSPLPESPSN-DALTESYFFRQPASKYSITQDSPR 145
Query: 99 TKSGATFTFAPEYCAAIN 46
S ++F P+ A+N
Sbjct: 146 VSSTIAYSFKPKASIALN 163
>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 450
Score = 31.9 bits (69), Expect = 0.076
Identities = 20/54 (37%), Positives = 30/54 (55%), Gaps = 1/54 (1%)
Frame = +2
Query: 428 SRTFLVTER-ITLADIIVYSTLLHAFQHVLDPTVRSKLVNVQRWFLTVGHQPQV 586
S TF+ + I++AD+ VY+ + H++ L KL NV RWF + HQ V
Sbjct: 134 SSTFIAQDSGISVADLAVYARI-HSYICGLSAKEGYKLNNVCRWFDFIQHQESV 186
>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
Eme1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 738
Score = 31.1 bits (67), Expect = 0.13
Identities = 18/62 (29%), Positives = 31/62 (50%)
Frame = +2
Query: 20 PENFRAYKALIAAQYSGANVKVAPDFVFGETNKTENFLKKFPAGKVPAFESADGKVLLTE 199
P F++ KA + QY+ A +FG +K +NF K+ ++ F S + +L+
Sbjct: 553 PNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRF-SFEHSILINT 611
Query: 200 SN 205
SN
Sbjct: 612 SN 613
>SPCC18.13 |||tRNA |Schizosaccharomyces pombe|chr 3|||Manual
Length = 421
Score = 29.1 bits (62), Expect = 0.53
Identities = 11/26 (42%), Positives = 16/26 (61%), Gaps = 3/26 (11%)
Frame = +3
Query: 237 CEVETWQHRH---VCCNGPHGLTVNC 305
C+ TW +H VCC+GP+ L +C
Sbjct: 11 CQFLTWNSKHNYIVCCSGPYLLGFSC 36
>SPCC1223.11 |ptc2||protein phosphatase 2C Ptc2 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 370
Score = 26.2 bits (55), Expect = 3.8
Identities = 17/57 (29%), Positives = 25/57 (43%)
Frame = +1
Query: 316 LRMGLSIFRHHAVQQTECRACKE*FTGCTATSGWTSFEPHFPGHGEDYSCRYYRLQH 486
L G+S + + + FT +++ TSF F GHG D +Y R QH
Sbjct: 22 LHFGVSHMQGWRISMEDAHCALLNFTDSNSSNPPTSFFGVFDGHGGDRVAKYCR-QH 77
>SPBC146.04 |||sulfhydryl oxidase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 192
Score = 26.2 bits (55), Expect = 3.8
Identities = 9/26 (34%), Positives = 18/26 (69%)
Frame = -1
Query: 167 RMLVLYPPGTSSRSFQSCWSHRIQNR 90
++L ++PP TSSR + W+ ++ N+
Sbjct: 118 KILDVHPPQTSSRKAATTWACKVHNQ 143
>SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein
kinase Ppk5 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 836
Score = 25.8 bits (54), Expect = 5.0
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -3
Query: 210 IALLSVSKTLPSALSNAGTLPAGNF 136
+ L+S S+TLP S + + P NF
Sbjct: 2 VGLISTSETLPKQESKSSSAPVSNF 26
>SPBC3E7.06c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 577
Score = 25.0 bits (52), Expect = 8.7
Identities = 19/60 (31%), Positives = 26/60 (43%)
Frame = -3
Query: 258 VARSPPRRSSFAT**AIALLSVSKTLPSALSNAGTLPAGNFFKKFSVLLVSPNTKSGATF 79
V + P R AT ++ +SVSK LP+ L N ++ S TK GA F
Sbjct: 67 VVSNSPHRQDAATTNTVSTVSVSKVLPALLLGVVLAALDN------TIVASTYTKIGAEF 120
>SPAC57A10.08c |||esterase/lipase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 364
Score = 25.0 bits (52), Expect = 8.7
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = -2
Query: 109 LTEYKIGGYFHVRPGILCGDQRLVSAEIF 23
L EYK+ +++RPGI+ + L E+F
Sbjct: 76 LNEYKVA--YYLRPGIIPLNHNLAKQEVF 102
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,746,420
Number of Sequences: 5004
Number of extensions: 57296
Number of successful extensions: 144
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 269634532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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