Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0010_C14
(556 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 101 5e-23
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 99 2e-22
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 88 9e-19
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 62 5e-11
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 59 4e-10
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 36 0.005
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 28 1.1
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 27 1.4
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 27 1.9
SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces pombe... 25 7.5
SPBC23E6.07c |rfc1||DNA replication factor C complex subunit Rfc... 25 9.9
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 101 bits (243), Expect = 5e-23
Identities = 52/134 (38%), Positives = 81/134 (60%)
Frame = +2
Query: 71 FQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKFTLHLKPDERISFEVFLPIYQ 250
+++AF LFD G G+I IGD LRA GQNPT +++ + L P E + E FL +
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTL-PAE-VDMEQFLQVLN 65
Query: 251 AISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDS 430
+ +F++G + FDKD G I ELR++L++LGEKLS++E+++LL+G
Sbjct: 66 RPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVK 125
Query: 431 QRNINYENFVHLIM 472
+NY +FV +I+
Sbjct: 126 DGMVNYHDFVQMIL 139
Score = 27.5 bits (58), Expect = 1.4
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +2
Query: 68 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 181
EF + FQ+FD G I V ++ L +LG+ + ++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEM 115
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 99 bits (238), Expect = 2e-22
Identities = 54/143 (37%), Positives = 84/143 (58%), Gaps = 3/143 (2%)
Frame = +2
Query: 50 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKFTLHLKPDER--IS 223
+++Q+AEF+EAF LFD DG I ++G +R+LGQ+PT ++++ + D I
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 224 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVE 403
F FL + K + D + E + FDKDGNG+I+ EL H+L++LGE+LS +EV
Sbjct: 67 FTEFLTMMAR--KMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVA 124
Query: 404 QLL-QGQEDSQRNINYENFVHLI 469
++ + D INYE F +I
Sbjct: 125 DMIREADTDGDGVINYEEFSRVI 147
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 87.8 bits (208), Expect = 9e-19
Identities = 49/142 (34%), Positives = 84/142 (59%), Gaps = 1/142 (0%)
Frame = +2
Query: 50 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKFTLHLKPDERISFE 229
S++Q E +EAF L+D DG I + +G LR+LG N T++++ K + L + I +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL--GDAIDEK 61
Query: 230 VFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQL 409
F+ +K R ++ ++I+ R FDKD +G+I +A+ + TLGEKLSD+EV+ +
Sbjct: 62 KFMSFVS--NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLM 119
Query: 410 LQGQEDSQR-NINYENFVHLIM 472
+Q + + + +Y +FV IM
Sbjct: 120 VQEADPTNSGSFDYYDFVQRIM 141
Score = 35.9 bits (79), Expect = 0.004
Identities = 16/65 (24%), Positives = 35/65 (53%)
Frame = +2
Query: 68 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKFTLHLKPDERISFEVFLPIY 247
E+ +AF++FD G I A+ D ++ LG+ ++++V+ P SF+ + +
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138
Query: 248 QAISK 262
+ ++K
Sbjct: 139 RIMAK 143
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 62.1 bits (144), Expect = 5e-11
Identities = 42/144 (29%), Positives = 67/144 (46%), Gaps = 3/144 (2%)
Frame = +2
Query: 50 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKFTLHLKPDER--IS 223
+E+Q + EAF+LFDS D I ++ A+RALG N +S+V K + +
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91
Query: 224 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVE 403
E F+ + K D + FD D G IS LR + L E + D E+E
Sbjct: 92 MEDFVRV--MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELE 149
Query: 404 QLLQGQE-DSQRNINYENFVHLIM 472
+++ + D IN + F+ ++M
Sbjct: 150 AMIEEFDLDQDGEINEQEFIAIMM 173
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 59.3 bits (137), Expect = 4e-10
Identities = 42/143 (29%), Positives = 65/143 (45%)
Frame = +2
Query: 41 AGYSEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKFTLHLKPDERI 220
A + Q+ E +EAF L D GDG I + L +L Q+ +E + + P I
Sbjct: 40 AQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESINPP--I 97
Query: 221 SFEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEV 400
+ FL ++ S ND +E FD +G I + +R LS++G+++ EV
Sbjct: 98 NLAAFLTAMGSMLCRIS--PRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEV 155
Query: 401 EQLLQGQEDSQRNINYENFVHLI 469
E +L+ S YE FV I
Sbjct: 156 ESILRSY-TSHGVFYYEKFVDAI 177
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 35.5 bits (78), Expect = 0.005
Identities = 15/57 (26%), Positives = 28/57 (49%)
Frame = +2
Query: 305 RHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQRNINYENFVHLIMQ 475
RHF+K + ++ E L++LG +E L +S+ + YE F ++M+
Sbjct: 494 RHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEGVTYERFTEIVME 550
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 27.9 bits (59), Expect = 1.1
Identities = 18/64 (28%), Positives = 30/64 (46%)
Frame = +2
Query: 68 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKFTLHLKPDERISFEVFLPIY 247
E +EAF LFD G I + + LG+N T+ ++ + ++S E F ++
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGKVSREEFAELW 72
Query: 248 QAIS 259
IS
Sbjct: 73 IHIS 76
Score = 27.5 bits (58), Expect = 1.4
Identities = 15/52 (28%), Positives = 26/52 (50%)
Frame = +2
Query: 311 FDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQRNINYENFVHL 466
FD G+I +LR + LGE L+ +++ QL+ + ++ E F L
Sbjct: 21 FDVTHKGYIDFEDLRRSCAQLGENLTKEQL-QLMLDLAGTNGKVSREEFAEL 71
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 27.5 bits (58), Expect = 1.4
Identities = 20/66 (30%), Positives = 37/66 (56%), Gaps = 4/66 (6%)
Frame = +2
Query: 227 EVFLPIYQAISKARSGDTANDFI--EGLRHFDKDGNGFISS-AELRHLLSTLGEK-LSDD 394
++F+ + I+K +G + I + RH DK GN F ++ EL +L +G+K L++
Sbjct: 643 DIFMSVTD-ITKIENGTKIYEEILQDCCRHIDKSGNEFTTAYLELLEVLLKVGQKYLAES 701
Query: 395 EVEQLL 412
+E +L
Sbjct: 702 LLEHIL 707
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 27.1 bits (57), Expect = 1.9
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = +2
Query: 335 ISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQRNINYENFVHLIMQG 478
I A++ LLSTL E D ++EQ+ Q + + Y +F+ ++QG
Sbjct: 42 IVKAQILFLLSTLREDQYDTKLEQIRQLINKNAPRV-YHHFLRRLIQG 88
>SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 727
Score = 25.0 bits (52), Expect = 7.5
Identities = 15/55 (27%), Positives = 26/55 (47%)
Frame = +2
Query: 122 VAQIGDALRALGQNPTESDVKKFTLHLKPDERISFEVFLPIYQAISKARSGDTAN 286
+ Q+ D LR G + + + T + ERI+ E ++A+ KA GD +
Sbjct: 23 IPQLKDILRVFGLRLSGTKAELITRIKQLIERIAIENNTTSWEALKKAIDGDVTS 77
>SPBC23E6.07c |rfc1||DNA replication factor C complex subunit
Rfc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 934
Score = 24.6 bits (51), Expect = 9.9
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = +2
Query: 374 GEKLSDDEVEQLLQGQEDSQRNI 442
G KLS V+QL+QG + R I
Sbjct: 558 GLKLSPQAVDQLVQGTQSDMRQI 580
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,233,546
Number of Sequences: 5004
Number of extensions: 45169
Number of successful extensions: 150
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 231978230
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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