Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0008_O17
(579 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.09c |paa1||protein phosphatase regulatory subunit Paa1|S... 159 4e-40
SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 41 1e-04
SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces pomb... 29 0.65
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 28 1.1
SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac... 28 1.1
SPBC725.08 |||transcription factor |Schizosaccharomyces pombe|ch... 27 1.5
SPAC144.09c |sfc2||RNA polymerase III transcription factor TFIII... 27 2.0
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 27 2.6
SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|... 26 3.5
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 26 4.6
SPAC22E12.11c |set3||histone lysine methyltransferase Set3|Schiz... 25 6.1
SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalyt... 25 8.0
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 25 8.0
SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr 2|||M... 25 8.0
SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar ... 25 8.0
SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein Vps33|S... 25 8.0
>SPAP8A3.09c |paa1||protein phosphatase regulatory subunit
Paa1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 590
Score = 159 bits (385), Expect = 4e-40
Identities = 86/191 (45%), Positives = 119/191 (62%), Gaps = 1/191 (0%)
Frame = +3
Query: 9 DDQDSVRXXXXXXXXXXXXXXPPE-DMEQLVMPTVRARAGDTSWRVRYMVADKFVELQQA 185
DDQDSVR + ++ ++ +R+ D+SWR RYMVA FV+L +
Sbjct: 216 DDQDSVRLLSFDIMVSLAEVLKSDSEIRHYLLQPLRSFVSDSSWRTRYMVAANFVKLAKV 275
Query: 186 VGPELARSDLAQIFQALLKDTEAEVRAAAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDL 365
VGP L + +L + F L+KDTE EVR A A ++ FC LDK I++ I+P I++L
Sbjct: 276 VGPSLIKDELIKPFVLLMKDTEQEVRRAIATQIPGFCELLDK----RIVLEEIIPVIQEL 331
Query: 366 VCDANQHVKSALASVIMGLSPIVGRQNTIEHLLPLFLTQLKDECPEVRLNIICNLECVNE 545
+ D QHV++AL I L+P +G++ T E+LLP+FL LKDE PEVRLNII LE VN+
Sbjct: 332 INDPAQHVRAALGMNIGALAPQLGKEKTTEYLLPMFLELLKDENPEVRLNIISKLEVVNK 391
Query: 546 VIGIQQLVQSL 578
V+GI+ L QSL
Sbjct: 392 VVGIELLSQSL 402
Score = 49.2 bits (112), Expect = 4e-07
Identities = 37/158 (23%), Positives = 70/158 (44%)
Frame = +3
Query: 87 EQLVMPTVRARAGDTSWRVRYMVADKFVELQQAVGPELARSDLAQIFQALLKDTEAEVRA 266
++L+ P V DT VR +A + + + + ++ + Q L+ D VRA
Sbjct: 283 DELIKPFVLLMK-DTEQEVRRAIATQIPGFCELLDKRIVLEEIIPVIQELINDPAQHVRA 341
Query: 267 AAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVIMGLSPIVGRQN 446
A + L K ++ M L +KD N V+ + S + ++ +VG +
Sbjct: 342 ALGMNIGALAPQLGKEKTTEYLLPMFLELLKD----ENPEVRLNIISKLEVVNKVVGIEL 397
Query: 447 TIEHLLPLFLTQLKDECPEVRLNIICNLECVNEVIGIQ 560
+ LLP +T +D+ VRL II + + + +G++
Sbjct: 398 LSQSLLPAIVTLAEDKQWRVRLAIIDYIPLLAQQLGVE 435
Score = 45.2 bits (102), Expect = 7e-06
Identities = 33/158 (20%), Positives = 62/158 (39%)
Frame = +3
Query: 78 EDMEQLVMPTVRARAGDTSWRVRYMVADKFVELQQAVGPELARSDLAQIFQALLKDTEAE 257
E + Q ++P + A D WRVR + D L Q +G E + + + L+D
Sbjct: 396 ELLSQSLLPAIVTLAEDKQWRVRLAIIDYIPLLAQQLGVEFFNEKMGNLCMSWLEDHVYS 455
Query: 258 VRAAAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVIMGLSPIVG 437
+R AA ++ L + I+P+ + N + I ++P +
Sbjct: 456 IREAAIKNLR----KLTEIFGLEWATETIIPKFLAMRSHPNYLYRMTTIFAISEIAPALN 511
Query: 438 RQNTIEHLLPLFLTQLKDECPEVRLNIICNLECVNEVI 551
+ + +LP + D P +R N+ E + V+
Sbjct: 512 AEVIEKQILPTLEQLVNDPIPNIRFNVAKAFEVLKPVL 549
Score = 27.1 bits (57), Expect = 2.0
Identities = 28/122 (22%), Positives = 50/122 (40%), Gaps = 1/122 (0%)
Frame = +3
Query: 150 MVADKFVELQQAVGPELARSDLAQIFQALLKD-TEAEVRAAAAGKVKDFCMNLDKAHQEH 326
+V DK V+ V L++ L Q F L++ + AE + A +C ++ +
Sbjct: 103 VVRDKAVDSLNKVCICLSQEQLEQYFVPLVQRLSTAEWFTSRASSAGLYCAAYSQS-ENP 161
Query: 327 IIMTMILPQIKDLVCDANQHVKSALASVIMGLSPIVGRQNTIEHLLPLFLTQLKDECPEV 506
+ + L D V+ A+ +V +Q I+ +PLF + D+ V
Sbjct: 162 AVKVSLRQSFSHLCHDEAPMVRRPAATNCAKFVFLVTKQEAIDEFIPLFNSLSNDDQDSV 221
Query: 507 RL 512
RL
Sbjct: 222 RL 223
>SPBC119.07 |ppk19||serine/threonine protein kinase
Ppk19|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1706
Score = 41.1 bits (92), Expect = 1e-04
Identities = 28/112 (25%), Positives = 55/112 (49%)
Frame = +3
Query: 234 LLKDTEAEVRAAAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVI 413
LL D+ + VR + + C+ KA +I++ ++ + D + ++ A I
Sbjct: 600 LLADSSSIVRRSLLNALAPLCVFFGKAKSNDLILSHLITYLND----TDWMLRCAFFESI 655
Query: 414 MGLSPIVGRQNTIEHLLPLFLTQLKDECPEVRLNIICNLECVNEVIGIQQLV 569
GLS +G ++ E++LPL L L D P V +++ + + E+ ++LV
Sbjct: 656 TGLSIFIGPRSVDEYILPLMLQALVDPEPAVLESVLGSFSGLIELHLFEKLV 707
Score = 29.9 bits (64), Expect = 0.28
Identities = 24/107 (22%), Positives = 42/107 (39%)
Frame = +3
Query: 93 LVMPTVRARAGDTSWRVRYMVADKFVELQQAVGPELARSDLAQIFQALLKDTEAEVRAAA 272
L++ + DT W +R + L +GP + + L D E V +
Sbjct: 631 LILSHLITYLNDTDWMLRCAFFESITGLSIFIGPRSVDEYILPLMLQALVDPEPAVLESV 690
Query: 273 AGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVI 413
G F ++ E +++ IL + LV N +++ A SVI
Sbjct: 691 LG---SFSGLIELHLFEKLVVVDILQLVLPLVAVPNAYIRRAALSVI 734
Score = 26.2 bits (55), Expect = 3.5
Identities = 14/65 (21%), Positives = 35/65 (53%), Gaps = 1/65 (1%)
Frame = +3
Query: 339 MILPQIKDLVCDAN-QHVKSALASVIMGLSPIVGRQNTIEHLLPLFLTQLKDECPEVRLN 515
++LP + + N + K S++ LS + ++ ++ +LP +T L+D+ +VR++
Sbjct: 431 VLLPIVLSTIRHVNTRESKINALSLVQILSRNICDESKLDTVLPFVMTLLRDQYADVRIS 490
Query: 516 IICNL 530
+ +
Sbjct: 491 ALITI 495
>SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 910
Score = 28.7 bits (61), Expect = 0.65
Identities = 23/68 (33%), Positives = 29/68 (42%), Gaps = 2/68 (2%)
Frame = +3
Query: 102 PTVRARAGDTSWRVRYMVADKFVELQQAVGPELARSD--LAQIFQALLKDTEAEVRAAAA 275
PTV D +V+ L V P LA++D L QI KD EVR +A
Sbjct: 651 PTVDVPDRDFLVTTLDLVSGIIQALGSQVSPLLAQADPPLGQIIGICAKDEVPEVRQSAY 710
Query: 276 GKVKDFCM 299
+ D CM
Sbjct: 711 ALLGDMCM 718
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 27.9 bits (59), Expect = 1.1
Identities = 15/38 (39%), Positives = 17/38 (44%), Gaps = 1/38 (2%)
Frame = +2
Query: 410 HYGPQP-HRRPTEHNRAPPASVPDTIERRMP*GPFEHH 520
H P P H P EH PP + MP PF+HH
Sbjct: 310 HMPPPPMHHEPGEH--MPPPPMHHEPGEHMPPPPFKHH 345
>SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit
Smc6|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1140
Score = 27.9 bits (59), Expect = 1.1
Identities = 24/98 (24%), Positives = 44/98 (44%), Gaps = 1/98 (1%)
Frame = +3
Query: 285 KDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVIMGLSPIVGRQNTIEHLL 464
++F L HQE + + P ++L N+H KS ++ + GLS G + + +
Sbjct: 1001 RNFTGKLVIKHQEEFLEPRVYPANRNLATAHNRHEKSKVS--VQGLS---GGEKSFATIC 1055
Query: 465 PLFLTQLKDECPEVRLN-IICNLECVNEVIGIQQLVQS 575
L CP L+ ++ VN ++ I+ +V S
Sbjct: 1056 MLLSIWEAMSCPLRCLDEFDVFMDAVNRLVSIKMMVDS 1093
>SPBC725.08 |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 609
Score = 27.5 bits (58), Expect = 1.5
Identities = 14/43 (32%), Positives = 23/43 (53%)
Frame = -2
Query: 251 LRILQQSLEYLGQIGTSQFWTNSLLQFNKLVGNHVPDAPGGVS 123
+R+ EYL Q+ T FWT+ ++ L+ + D+P G S
Sbjct: 371 IRLSDLYAEYLRQVATFDFWTSK--EYESLIA-LLQDSPAGYS 410
>SPAC144.09c |sfc2||RNA polymerase III transcription factor
TFIIIA|Schizosaccharomyces pombe|chr 1|||Manual
Length = 374
Score = 27.1 bits (57), Expect = 2.0
Identities = 20/65 (30%), Positives = 30/65 (46%), Gaps = 2/65 (3%)
Frame = -2
Query: 248 RILQQSLEYLGQIGTSQFWTNSLLQFNKLVGNHVPDAP--GGVSGTSSHRRHHQLLHVLG 75
+I+ S + +G F S LQ N + HVP G T++H RHH +LH
Sbjct: 171 KIISYSCPHESCVGHEGFEKWSQLQ-NHIREAHVPSCSICGRQFKTAAHLRHHVVLHQTT 229
Query: 74 RQESR 60
+E +
Sbjct: 230 LEERK 234
>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1067
Score = 26.6 bits (56), Expect = 2.6
Identities = 26/96 (27%), Positives = 50/96 (52%), Gaps = 4/96 (4%)
Frame = +3
Query: 210 DLAQIFQALLKDTEAEVRAAAAGKVKDFC-MNLDKAHQEHIIMTMILPQIKDLVCDANQH 386
D+ +F A L+D+E EVR+ AA + C + + E++ IL +++ +
Sbjct: 894 DVFSLFMAALEDSEGEVRSNAAYSMGLLCQFSTEDLSSEYL---NILQKLQPFF--TQEV 948
Query: 387 VKSALASVIMGLSP-IVGRQNTI--EHLLPLFLTQL 485
++AL + I +S I+ QN I + +LP+ ++L
Sbjct: 949 FRTALDNAIGCISRLILHNQNAIPVDQVLPIVFSKL 984
>SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein
5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 736
Score = 26.2 bits (55), Expect = 3.5
Identities = 15/57 (26%), Positives = 25/57 (43%)
Frame = -3
Query: 301 FMQKSLTFPAAAARTSASVSFSRAWNIWAKSERASSGPTACCNSTNLSATMYRTRQE 131
FM+ L + +S S S+ WN + + S T +L+A Y+T+ E
Sbjct: 135 FMESELQTKLSTINSSPFKSLSQYWNAAYNKKESISSSTNSNIKLHLAARKYQTKPE 191
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 25.8 bits (54), Expect = 4.6
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = -1
Query: 63 PPPQRTPRRPADAQ 22
PPPQ TP + ADA+
Sbjct: 177 PPPQETPTKSADAE 190
>SPAC22E12.11c |set3||histone lysine methyltransferase
Set3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 859
Score = 25.4 bits (53), Expect = 6.1
Identities = 18/66 (27%), Positives = 26/66 (39%)
Frame = -3
Query: 310 LSRFMQKSLTFPAAAARTSASVSFSRAWNIWAKSERASSGPTACCNSTNLSATMYRTRQE 131
LS + L+F A++ + FSR + RAS+ LS T T
Sbjct: 364 LSEVLSTFLSFNECASQDKKNCVFSRVTKYIKAARRASTANRVSVAKDRLSLTPSSTPST 423
Query: 130 VSPARA 113
SPA +
Sbjct: 424 PSPAES 429
>SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalytic
subunit Pka1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 512
Score = 25.0 bits (52), Expect = 8.0
Identities = 15/50 (30%), Positives = 24/50 (48%), Gaps = 1/50 (2%)
Frame = -2
Query: 170 NKLVGNHVPDAPGGVSGTSSHRRH-HQLLHVLGRQESRHRSARLGGQQTH 24
+ L+ + +P++ S SSH+RH LG + RSA G + H
Sbjct: 119 DNLIPSPLPESASRSSSQSSHQRHSRDGRGELGSEHGERRSAMDGLRDRH 168
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 25.0 bits (52), Expect = 8.0
Identities = 13/42 (30%), Positives = 16/42 (38%)
Frame = +2
Query: 392 IGPRFRHYGPQPHRRPTEHNRAPPASVPDTIERRMP*GPFEH 517
I P+ P P P + PP V +I MP P H
Sbjct: 723 IKPQVTPAPPTPAPTPAVKHHPPPPPVRSSISPSMPPAPLTH 764
>SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 685
Score = 25.0 bits (52), Expect = 8.0
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = +3
Query: 330 IMTMILPQIKDLVCDANQHVKSALASVIMGLSP 428
I T+ + I+ L D H KS MGL+P
Sbjct: 9 IDTLAINTIRTLAVDTTAHAKSGHPGAPMGLAP 41
>SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar
membrane transporter Hmt1|Schizosaccharomyces pombe|chr
3|||Manual
Length = 830
Score = 25.0 bits (52), Expect = 8.0
Identities = 9/29 (31%), Positives = 14/29 (48%)
Frame = +1
Query: 10 MIKILCVCWPPRRALRWRLSCRPRTWSSW 96
+I LC C+ + WR R + +SW
Sbjct: 412 LIMTLCYCYVTVKITSWRTEARRKMVNSW 440
>SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein
Vps33|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 25.0 bits (52), Expect = 8.0
Identities = 16/61 (26%), Positives = 27/61 (44%)
Frame = +3
Query: 213 LAQIFQALLKDTEAEVRAAAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVK 392
L++I + L D E +A +++DF L EH + + + LV QH K
Sbjct: 294 LSKIARKLSSDFEGRRQAKTVNQIRDFVSKLGSLQSEHTSLNIHTGLAETLV----QHTK 349
Query: 393 S 395
+
Sbjct: 350 N 350
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,316,166
Number of Sequences: 5004
Number of extensions: 46704
Number of successful extensions: 166
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 248115846
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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