Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0008_N21
(427 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17G6.12 |cul1|pcu1|cullin 1|Schizosaccharomyces pombe|chr 1|... 158 4e-40
SPAC24H6.03 |cul3|pcu3|cullin 3|Schizosaccharomyces pombe|chr 1|... 96 2e-21
SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces pombe... 86 2e-18
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo... 30 0.13
SPAC26A3.10 |||Arf GAP protein|Schizosaccharomyces pombe|chr 1||... 29 0.40
SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4 |Schizosacc... 29 0.40
SPBC1703.12 |ubp9||ubiquitin C-terminal hydrolase Ubp9|Schizosac... 28 0.70
SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3 |Schizosaccha... 26 2.8
SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium tra... 26 2.8
SPAC1B3.07c |vps28||ESCRT I complex subunit Vps28|Schizosaccharo... 25 4.9
SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||M... 25 4.9
SPBC2G5.03 |||ATP binding protein|Schizosaccharomyces pombe|chr ... 24 8.6
>SPAC17G6.12 |cul1|pcu1|cullin 1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 767
Score = 158 bits (383), Expect = 4e-40
Identities = 74/134 (55%), Positives = 100/134 (74%)
Frame = +1
Query: 7 EAGFVAALDKACGRFINTNAVTKAANSSSKSPELLAKYCDLLLKKSSKNPEEAELEDTLN 186
+ F +LD A +N N V + SSS+SPELLAKY D +L+KS+KN + ++ED L+
Sbjct: 385 DTDFTKSLDTAFRELVNRNVVCQ--RSSSRSPELLAKYADSILRKSNKNVDIDDVEDCLS 442
Query: 187 QVMVVFKYIEDKDVFQKFYSKMLAKRLVQHMSASDDAEASMISKLKQACGFEYTSKLQRM 366
++++F+Y+EDKDVFQ FY+K+LAKRLV S S DAE+SM+SKLK+ CGFEYTSKLQRM
Sbjct: 443 SIIIIFRYVEDKDVFQNFYTKLLAKRLVNGTSNSQDAESSMLSKLKEVCGFEYTSKLQRM 502
Query: 367 FHDIGVSNDLNENF 408
F DI +S ++ E F
Sbjct: 503 FQDISLSQEITEAF 516
>SPAC24H6.03 |cul3|pcu3|cullin 3|Schizosaccharomyces pombe|chr
1|||Manual
Length = 785
Score = 96.3 bits (229), Expect = 2e-21
Identities = 45/122 (36%), Positives = 77/122 (63%)
Frame = +1
Query: 58 TNAVTKAANSSSKSPELLAKYCDLLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQK 237
++A + +++PE ++ + D LKK ++ E +E TL + +F++I +KDVF+K
Sbjct: 408 SDAFVTFVDGYTRAPEYISLFIDDNLKKDARKAIEGSIEATLQNSVTLFRFISEKDVFEK 467
Query: 238 FYSKMLAKRLVQHMSASDDAEASMISKLKQACGFEYTSKLQRMFHDIGVSNDLNENFRKH 417
+Y LAKRL+ + S S DAE MIS+LKQ G +T KL+ MF+D+ +S +L + ++ +
Sbjct: 468 YYKTHLAKRLLNNRSISSDAELGMISRLKQEAGNVFTQKLEGMFNDMNLSQELLQEYKHN 527
Query: 418 MA 423
A
Sbjct: 528 SA 529
>SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 734
Score = 86.2 bits (204), Expect = 2e-18
Identities = 46/141 (32%), Positives = 77/141 (54%), Gaps = 1/141 (0%)
Frame = +1
Query: 4 HEAGFVAALDKACGRFINTNAVTKAANSSSKSP-ELLAKYCDLLLKKSSKNPEEAELEDT 180
H+ A+ KA FIN A S ++P L+AKY D LL+ + L++
Sbjct: 352 HDETLSYAMRKAFETFING-----AKGSQREAPARLIAKYIDYLLRVGEQASGGKPLKEV 406
Query: 181 LNQVMVVFKYIEDKDVFQKFYSKMLAKRLVQHMSASDDAEASMISKLKQACGFEYTSKLQ 360
++++ +F+YI KD+F+ +Y +AKRL+ + SAS E ++ LK+ CG ++T L+
Sbjct: 407 FSEILDLFRYIASKDIFEAYYKLDIAKRLLLNKSASAQNELMLLDMLKKTCGSQFTHSLE 466
Query: 361 RMFHDIGVSNDLNENFRKHMA 423
MF D+ +S + +FR A
Sbjct: 467 GMFRDVNISKEFTSSFRHSKA 487
>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1811
Score = 30.3 bits (65), Expect = 0.13
Identities = 20/57 (35%), Positives = 30/57 (52%)
Frame = +1
Query: 244 SKMLAKRLVQHMSASDDAEASMISKLKQACGFEYTSKLQRMFHDIGVSNDLNENFRK 414
+K+L+ +V+HM EA + +KL FE S L+ HD NDL + F+K
Sbjct: 1672 AKILSSGIVEHMPNLLSQEA-LCAKLYFYTAFECMSSLKSDSHDTEEYNDLMDVFQK 1727
>SPAC26A3.10 |||Arf GAP protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 923
Score = 28.7 bits (61), Expect = 0.40
Identities = 20/69 (28%), Positives = 32/69 (46%)
Frame = +1
Query: 211 IEDKDVFQKFYSKMLAKRLVQHMSASDDAEASMISKLKQACGFEYTSKLQRMFHDIGVSN 390
+E K K+Y K + KR+VQ SD +AS ++ +K + S M DI + +
Sbjct: 241 LEQKSSLMKYYCKKIMKRIVQ---LSDAYDASQVAVMKLSETLSEASNSTSMNMDILLDS 297
Query: 391 DLNENFRKH 417
L + H
Sbjct: 298 YLTKAMDIH 306
>SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 488
Score = 28.7 bits (61), Expect = 0.40
Identities = 23/84 (27%), Positives = 43/84 (51%), Gaps = 2/84 (2%)
Frame = +1
Query: 67 VTKAANSSSKSPELLAKYCDLLLKKSSKNPE--EAELEDTLNQVMVVFKYIEDKDVFQKF 240
+++ ++ E LAK+ D + SSK E E E+ ++ +Q+ + +K FQ+
Sbjct: 115 ISRLTKERDEAREALAKFSDNIGTVSSKTIEVQEVEMGESDDQLKTSLRSTVEK-TFQEL 173
Query: 241 YSKMLAKRLVQHMSASDDAEASMI 312
SK +L Q A+DDA + ++
Sbjct: 174 SSKRKRTKL-QPKWATDDAVSQLL 196
>SPBC1703.12 |ubp9||ubiquitin C-terminal hydrolase
Ubp9|Schizosaccharomyces pombe|chr 2|||Manual
Length = 585
Score = 27.9 bits (59), Expect = 0.70
Identities = 22/87 (25%), Positives = 43/87 (49%), Gaps = 5/87 (5%)
Frame = +1
Query: 55 NTNAVTKAANSSSKSPELLAK---YCDLL--LKKSSKNPEEAELEDTLNQVMVVFKYIED 219
N +VT S S S L +K +CD+ L+++ K + +L L+ + FKY E
Sbjct: 266 NHTSVTSCLRSFSASEMLSSKNKFHCDVCKSLQEAEKRMKIKKLPKILSLHLKRFKYNET 325
Query: 220 KDVFQKFYSKMLAKRLVQHMSASDDAE 300
++ K + ++ ++ + ++DAE
Sbjct: 326 QEGHDKLFYTIVFTNEMRLFTTTEDAE 352
>SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 578
Score = 25.8 bits (54), Expect = 2.8
Identities = 15/35 (42%), Positives = 22/35 (62%)
Frame = +1
Query: 226 VFQKFYSKMLAKRLVQHMSASDDAEASMISKLKQA 330
+F+ F KM AKR V+ + S D EAS+ K K++
Sbjct: 42 LFKAFLLKM-AKRSVEELKRSADEEASVKRKEKKS 75
Score = 24.2 bits (50), Expect = 8.6
Identities = 17/62 (27%), Positives = 30/62 (48%), Gaps = 2/62 (3%)
Frame = +1
Query: 247 KMLAKRLVQHMSAS--DDAEASMISKLKQACGFEYTSKLQRMFHDIGVSNDLNENFRKHM 420
+ML L +H+S+ DD + K+A E T L R ++ +G+ D+++ R
Sbjct: 401 RMLDNLLRKHLSSGGKDDKILIFVLYKKEAARVEGT--LARKYNVVGIHGDMSQGARLQA 458
Query: 421 AN 426
N
Sbjct: 459 LN 460
>SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium
transporting Cta4 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1211
Score = 25.8 bits (54), Expect = 2.8
Identities = 10/44 (22%), Positives = 21/44 (47%), Gaps = 1/44 (2%)
Frame = -1
Query: 400 RLGRSRHRYRGTSSAICL-CIQNRRPASVLRSSKPRRRRWPTYV 272
+ G +++ G ++C C+ RP L +P+ + TY+
Sbjct: 1017 KFGDTQYTISGMLMSVCFYCVSRARPLETLSKERPQAGIFNTYI 1060
>SPAC1B3.07c |vps28||ESCRT I complex subunit
Vps28|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 25.0 bits (52), Expect = 4.9
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = -3
Query: 371 WNILCNLLVYSKPQACFSFEIIEASASSLADICWTSRL 258
+N C LL+ + ++CFS E + + S D C RL
Sbjct: 58 FNSTCELLI-QQWESCFSDERVTQAFGSFEDFCSKYRL 94
>SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1317
Score = 25.0 bits (52), Expect = 4.9
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = +3
Query: 225 RVPKVLQQNARQAARPTYVGQRRRRGFDDLKTE 323
R K +Q+NA A R +G R R +DL E
Sbjct: 965 RYMKAIQENAESAVRQLLLGVRERFLGEDLYAE 997
>SPBC2G5.03 |||ATP binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 335
Score = 24.2 bits (50), Expect = 8.6
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = -1
Query: 388 SRHRYRGTSSAICLCIQNRRPASVL 314
S +RGT+ A+ ++N RP+S+L
Sbjct: 238 SPEAFRGTARAMIKQLENIRPSSIL 262
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.128 0.353
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,830,766
Number of Sequences: 5004
Number of extensions: 36897
Number of successful extensions: 145
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 152416050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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