Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0008_M18
(515 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 272 2e-74
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 268 3e-73
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 133 2e-32
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 100 1e-22
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate... 29 0.31
SPBC2D10.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 26 2.9
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 26 3.8
SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 26 3.8
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 25 5.1
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 25 8.9
SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1 |Schizosa... 25 8.9
SPBC428.07 |meu6||meiotic chromosome segregation protein Meu6|Sc... 25 8.9
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 272 bits (667), Expect = 2e-74
Identities = 122/169 (72%), Positives = 144/169 (85%)
Frame = +2
Query: 8 DRIRKLADQCTGLQXFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVST 187
+RIR++AD C+GLQ FL+FH LL+ERL+++YGKKS L+F++YPAPQVST
Sbjct: 124 ERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVST 183
Query: 188 AVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSIT 367
+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+VSSIT
Sbjct: 184 SVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSIT 243
Query: 368 ASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSV 514
ASLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++SA KA+HE SV
Sbjct: 244 ASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSV 292
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 268 bits (658), Expect = 3e-73
Identities = 120/169 (71%), Positives = 144/169 (85%)
Frame = +2
Query: 8 DRIRKLADQCTGLQXFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVST 187
D+IR++AD C+GLQ FL+FH LL+ERL+++Y KKSKL+F++YPAPQVST
Sbjct: 120 DKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVST 179
Query: 188 AVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSIT 367
+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSIT
Sbjct: 180 SVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSIT 239
Query: 368 ASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSV 514
ASLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++SA KA+HE SV
Sbjct: 240 ASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSV 288
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 133 bits (321), Expect = 2e-32
Identities = 61/169 (36%), Positives = 98/169 (57%)
Frame = +2
Query: 8 DRIRKLADQCTGLQXFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVST 187
D +R+ A+ C LQ F + H LL+ ++ +Y + F++ PAP+ S
Sbjct: 118 DVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSD 177
Query: 188 AVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSIT 367
VVEPYN+ L+ H +E+SD F +DNEA+ I L I+ P+Y +LN L+ +++ +T
Sbjct: 178 TVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVT 237
Query: 368 ASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSV 514
S RF G LN DL + N+VP+PR+HF +V +AP+ + + + +SV
Sbjct: 238 TSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSV 286
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 100 bits (239), Expect = 1e-22
Identities = 52/156 (33%), Positives = 81/156 (51%), Gaps = 1/156 (0%)
Frame = +2
Query: 8 DRIRKLADQCTGLQXFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 184
D I + AD L+ F + H L+ERL+ Y KK ++++P Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 185 TAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSI 364
VV+PYNS+L ++D ++DN A+ I L + PT+ N+L+ ++S+
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSAS 239
Query: 365 TASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAP 472
T +LR+ G +N DL +L+P PR HF L +Y P
Sbjct: 240 TTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTP 275
>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
transferase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 453
Score = 29.5 bits (63), Expect = 0.31
Identities = 11/38 (28%), Positives = 18/38 (47%)
Frame = +3
Query: 246 IVLSWSTMKPSMTSAAVTWILNARPTPTSIVLSARSYH 359
++ S+ T KP + TW +N R P S+ + H
Sbjct: 37 VIASYPTFKPVVNERCGTWYVNRRHAPISVYFKSTDGH 74
>SPBC2D10.16 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 135
Score = 26.2 bits (55), Expect = 2.9
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = -2
Query: 400 DIEGAVETQRGGNR*YDLADKTIEVGVGRAFN 305
DIE E GN+ ++L +K I++ G+ FN
Sbjct: 58 DIEAFAEYALDGNKYFNLINKIIDMQDGKRFN 89
>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 973
Score = 25.8 bits (54), Expect = 3.8
Identities = 15/58 (25%), Positives = 24/58 (41%)
Frame = +3
Query: 258 WSTMKPSMTSAAVTWILNARPTPTSIVLSARSYHRLPPLCVSTAPSMSI*RSSRPTWC 431
W T KP + A W ++ + + + S +PP S A + + RP WC
Sbjct: 428 WRTHKPLILRATPQWFISLENERKTAIKALDSVKMIPP--NSRARLLGF-LNGRPEWC 482
>SPBC1826.01c |mot1||TATA-binding protein associated factor
Mot1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1953
Score = 25.8 bits (54), Expect = 3.8
Identities = 13/21 (61%), Positives = 13/21 (61%)
Frame = +3
Query: 303 ILNARPTPTSIVLSARSYHRL 365
I NAR T V S RSYHRL
Sbjct: 1499 IKNARAKLTKAVKSLRSYHRL 1519
>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1727
Score = 25.4 bits (53), Expect = 5.1
Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 1/68 (1%)
Frame = -2
Query: 217 KDRVVGLYNSSGHLGCGIDSELQFRLLAVVNGESLHEERSETRTGATTERVEDEEXL-EA 41
KD++ L N + HLG + S Q + E + + +++ RV++E + E
Sbjct: 146 KDQIEALQNENSHLGEQVQSAHQAL-------SDIEERKKQHMFASSSSRVKEEILVQEK 198
Query: 40 CALVSEFA 17
ALVS+ A
Sbjct: 199 SALVSDLA 206
>SPAC1805.15c |pub2||ubiquitin-protein ligase
Pub2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 671
Score = 24.6 bits (51), Expect = 8.9
Identities = 9/23 (39%), Positives = 13/23 (56%)
Frame = +1
Query: 154 VRYLSRTPGVHCCCRALQLYPYN 222
V ++S+ P H C L + PYN
Sbjct: 627 VGHISQLPKAHTCFNRLDIPPYN 649
>SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 24.6 bits (51), Expect = 8.9
Identities = 13/48 (27%), Positives = 23/48 (47%)
Frame = -2
Query: 166 IDSELQFRLLAVVNGESLHEERSETRTGATTERVEDEEXLEACALVSE 23
+D E++ +LLA+ E E + E ++EE LE + + E
Sbjct: 445 VDPEIEAKLLALDEEEERLEREGYYDSDQEIEDADEEEVLEKASRIRE 492
>SPBC428.07 |meu6||meiotic chromosome segregation protein
Meu6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 651
Score = 24.6 bits (51), Expect = 8.9
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = +2
Query: 389 ALNVDLTEFQTNLVPYPRIHFPLVT 463
A +V++ E +TN +P HFP T
Sbjct: 368 AQSVEVPENETNQIPTTEEHFPATT 392
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,139,654
Number of Sequences: 5004
Number of extensions: 42028
Number of successful extensions: 148
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 208287218
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -