Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0008_L13
(431 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 113 8e-27
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 113 1e-26
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 113 1e-26
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 69 4e-13
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 56 2e-09
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 41 7e-05
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 38 7e-04
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 34 0.008
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 34 0.008
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 33 0.025
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 29 0.31
SPCC63.03 |||DNAJ domain protein, DNAJC11 family|Schizosaccharom... 27 0.93
SPBC23E6.07c |rfc1||DNA replication factor C complex subunit Rfc... 25 3.8
SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharo... 25 3.8
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 5.0
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 25 5.0
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 25 6.6
SPAC2E1P3.04 |||copper amine oxidase |Schizosaccharomyces pombe|... 24 8.7
SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccha... 24 8.7
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 24 8.7
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 113 bits (273), Expect = 8e-27
Identities = 53/76 (69%), Positives = 56/76 (73%)
Frame = +2
Query: 203 EAHEMGKGSFKYAWVLDKLNAERERGITTDIALWKFETGKYYVTIIDARGHRDXXXXXXX 382
EA E+GKGSFKYAWVLDKL AERERGIT DIALWKFET KY VT+IDA GHRD
Sbjct: 45 EATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMIT 104
Query: 383 XXXXXDCAVLIVAAGT 430
DCAVLI+ GT
Sbjct: 105 GTSQADCAVLIIGGGT 120
Score = 98.3 bits (234), Expect = 4e-22
Identities = 57/109 (52%), Positives = 65/109 (59%)
Frame = +1
Query: 70 MGKEKVHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK*SP*NG*GFLQIRLGI 249
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK + G G + +
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 250 GQTKCRA*AWYHHRYCSLEVRNRQILCHHHRRSWTQRFIKNMITGTSQA 396
+ K + + + FIKNMITGTSQA
Sbjct: 61 DKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQA 109
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 113 bits (272), Expect = 1e-26
Identities = 52/76 (68%), Positives = 56/76 (73%)
Frame = +2
Query: 203 EAHEMGKGSFKYAWVLDKLNAERERGITTDIALWKFETGKYYVTIIDARGHRDXXXXXXX 382
EA E+GKGSFKYAWVLDKL AERERGIT DIALWKFET KY VT+IDA GHRD
Sbjct: 45 EATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMIT 104
Query: 383 XXXXXDCAVLIVAAGT 430
DCA+LI+ GT
Sbjct: 105 GTSQADCAILIIGGGT 120
Score = 98.3 bits (234), Expect = 4e-22
Identities = 57/109 (52%), Positives = 65/109 (59%)
Frame = +1
Query: 70 MGKEKVHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK*SP*NG*GFLQIRLGI 249
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK + G G + +
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 250 GQTKCRA*AWYHHRYCSLEVRNRQILCHHHRRSWTQRFIKNMITGTSQA 396
+ K + + + FIKNMITGTSQA
Sbjct: 61 DKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQA 109
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 113 bits (272), Expect = 1e-26
Identities = 52/76 (68%), Positives = 56/76 (73%)
Frame = +2
Query: 203 EAHEMGKGSFKYAWVLDKLNAERERGITTDIALWKFETGKYYVTIIDARGHRDXXXXXXX 382
EA E+GKGSFKYAWVLDKL AERERGIT DIALWKFET KY VT+IDA GHRD
Sbjct: 45 EATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMIT 104
Query: 383 XXXXXDCAVLIVAAGT 430
DCA+LI+ GT
Sbjct: 105 GTSQADCAILIIGGGT 120
Score = 98.3 bits (234), Expect = 4e-22
Identities = 57/109 (52%), Positives = 65/109 (59%)
Frame = +1
Query: 70 MGKEKVHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK*SP*NG*GFLQIRLGI 249
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK + G G + +
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 250 GQTKCRA*AWYHHRYCSLEVRNRQILCHHHRRSWTQRFIKNMITGTSQA 396
+ K + + + FIKNMITGTSQA
Sbjct: 61 DKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQA 109
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 68.5 bits (160), Expect = 4e-13
Identities = 34/73 (46%), Positives = 40/73 (54%)
Frame = +2
Query: 200 SEAHEMGKGSFKYAWVLDKLNAERERGITTDIALWKFETGKYYVTIIDARGHRDXXXXXX 379
+EA GKGSF YAW+LD ER RG+T D+A FE+ K I DA GHRD
Sbjct: 214 NEAANSGKGSFSYAWLLDTTEEERARGVTMDVASTTFESDKKIYEIGDAPGHRDFISGMI 273
Query: 380 XXXXXXDCAVLIV 418
D AVL+V
Sbjct: 274 AGASSADFAVLVV 286
Score = 42.3 bits (95), Expect = 3e-05
Identities = 18/45 (40%), Positives = 31/45 (68%)
Frame = +1
Query: 91 INIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK*SP*NG*G 225
+++VV GHVDSGKST G ++++ G I+ R+++K + +G G
Sbjct: 178 VHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKG 222
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 56.0 bits (129), Expect = 2e-09
Identities = 23/40 (57%), Positives = 32/40 (80%)
Frame = +1
Query: 82 KVHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK 201
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E+
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIER 275
Score = 52.0 bits (119), Expect = 4e-08
Identities = 25/74 (33%), Positives = 38/74 (51%)
Frame = +2
Query: 203 EAHEMGKGSFKYAWVLDKLNAERERGITTDIALWKFETGKYYVTIIDARGHRDXXXXXXX 382
EA E GK S+ +W LD + ERE+G T ++ FET +++DA GH+
Sbjct: 276 EAKEAGKESWYLSWALDSTSEEREKGKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMIN 335
Query: 383 XXXXXDCAVLIVAA 424
D VL+++A
Sbjct: 336 GASQADIGVLVISA 349
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 41.1 bits (92), Expect = 7e-05
Identities = 21/71 (29%), Positives = 34/71 (47%)
Frame = +2
Query: 212 EMGKGSFKYAWVLDKLNAERERGITTDIALWKFETGKYYVTIIDARGHRDXXXXXXXXXX 391
++G+ SF +DK E+ RGIT A ++ET + +D GH D
Sbjct: 79 DLGQASFMDYSQIDKAPEEKARGITISSAHVEYETANRHYAHVDCPGHADYIKNMITGAA 138
Query: 392 XXDCAVLIVAA 424
D A+++V+A
Sbjct: 139 TMDGAIIVVSA 149
Score = 34.7 bits (76), Expect = 0.006
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +1
Query: 76 KEKVHINIVVIGHVDSGKSTTTGHLIYKC 162
++K H+NI IGHVD GK+T T I KC
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC 76
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 37.9 bits (84), Expect = 7e-04
Identities = 21/58 (36%), Positives = 27/58 (46%)
Frame = +2
Query: 245 VLDKLNAERERGITTDIALWKFETGKYYVTIIDARGHRDXXXXXXXXXXXXDCAVLIV 418
V+D L AER+RGIT + A F + +ID GH D D AV I+
Sbjct: 67 VMDYLPAERQRGITINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAII 124
Score = 28.7 bits (61), Expect = 0.40
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +1
Query: 94 NIVVIGHVDSGKSTTTGHLIYKCG 165
N+ +I H+D+GK+T T ++Y G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 34.3 bits (75), Expect = 0.008
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +1
Query: 70 MGKEKVHINIVVIGHVDSGKSTTTGHLIYKCG 165
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 34.3 bits (75), Expect = 0.008
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +1
Query: 70 MGKEKVHINIVVIGHVDSGKSTTTGHLIYKCG 165
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 32.7 bits (71), Expect = 0.025
Identities = 20/63 (31%), Positives = 28/63 (44%), Gaps = 4/63 (6%)
Frame = +2
Query: 248 LDKLNAERERGIT----TDIALWKFETGKYYVTIIDARGHRDXXXXXXXXXXXXDCAVLI 415
LDKL ER RGIT T ++ + Y + +ID GH D + +L+
Sbjct: 95 LDKLEVERRRGITVKAQTCSMIYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILL 154
Query: 416 VAA 424
V A
Sbjct: 155 VDA 157
Score = 24.6 bits (51), Expect = 6.6
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +1
Query: 94 NIVVIGHVDSGKSTTTGHLIYKCGGIDK 177
N VI H+D GKST + ++ G I++
Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINE 87
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 29.1 bits (62), Expect = 0.31
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +1
Query: 94 NIVVIGHVDSGKSTTTGHLIYKCGGI 171
NI + H+DSGK+T T ++Y G I
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI 86
Score = 24.2 bits (50), Expect = 8.7
Identities = 12/34 (35%), Positives = 15/34 (44%)
Frame = +2
Query: 323 YYVTIIDARGHRDXXXXXXXXXXXXDCAVLIVAA 424
Y + IID GH D D AVL++ A
Sbjct: 145 YNINIIDTPGHIDFTIEVERALRVLDGAVLVLCA 178
>SPCC63.03 |||DNAJ domain protein, DNAJC11
family|Schizosaccharomyces pombe|chr 3|||Manual
Length = 642
Score = 27.5 bits (58), Expect = 0.93
Identities = 15/40 (37%), Positives = 21/40 (52%)
Frame = -3
Query: 312 SNFQRAISVVIPRSRSAFSLSNTQAYLKEPLPISWASLLE 193
S F ISV +P FSL+ ++ K LPI W S+ +
Sbjct: 403 STFGVNISVGVPTGSITFSLNWSRLGQKISLPIMWCSVFD 442
>SPBC23E6.07c |rfc1||DNA replication factor C complex subunit
Rfc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 934
Score = 25.4 bits (53), Expect = 3.8
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +3
Query: 309 SKPANTMSPSSTLVDTEIHQEHDHWNV 389
S AN+ S S LVD+ IH HW++
Sbjct: 682 SSAANSFSDSD-LVDSMIHGPQQHWSL 707
>SPAC3F10.16c |||GTP binding protein,
HSR1-related|Schizosaccharomyces pombe|chr 1|||Manual
Length = 616
Score = 25.4 bits (53), Expect = 3.8
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = +3
Query: 321 NTMSPSSTLVDTEIHQEHDH 380
NT+S S LVD E QE+ H
Sbjct: 504 NTLSAESQLVDDEYFQENPH 523
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 25.0 bits (52), Expect = 5.0
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +1
Query: 64 PKMGKEKVHINIVVIGHVDSGKST 135
P + + INI IGHV GKST
Sbjct: 15 PAIISRQATINIGTIGHVAHGKST 38
>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 732
Score = 25.0 bits (52), Expect = 5.0
Identities = 8/20 (40%), Positives = 14/20 (70%), Gaps = 1/20 (5%)
Frame = +1
Query: 283 HHRY-CSLEVRNRQILCHHH 339
HH++ C L V+N+++ H H
Sbjct: 196 HHKFFCDLCVKNKKVFTHEH 215
>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1133
Score = 24.6 bits (51), Expect = 6.6
Identities = 9/33 (27%), Positives = 22/33 (66%)
Frame = +3
Query: 297 LSGSSKPANTMSPSSTLVDTEIHQEHDHWNVSG 395
+ SKP N ++ ++++VD + + +H++ N +G
Sbjct: 27 VEADSKP-NDLTAANSIVDLKTNSQHENANAAG 58
>SPAC2E1P3.04 |||copper amine oxidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 712
Score = 24.2 bits (50), Expect = 8.7
Identities = 9/25 (36%), Positives = 14/25 (56%)
Frame = +3
Query: 315 PANTMSPSSTLVDTEIHQEHDHWNV 389
PA + PS + +E+ Q H H N+
Sbjct: 656 PALDVPPSKNVTTSEVKQAHHHGNL 680
>SPAC2G11.11c |prh1||ATP-dependent RNA helicase
Prh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 719
Score = 24.2 bits (50), Expect = 8.7
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = +1
Query: 97 IVVIGHVDSGKSTTTGHLIYKC 162
IVV+G SGKST + +C
Sbjct: 115 IVVVGETGSGKSTQIPQFLNEC 136
>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 24.2 bits (50), Expect = 8.7
Identities = 8/22 (36%), Positives = 13/22 (59%)
Frame = +1
Query: 100 VVIGHVDSGKSTTTGHLIYKCG 165
+V+GH GK+T +Y+ G
Sbjct: 45 IVLGHTGCGKTTQIPQFLYEAG 66
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,757,137
Number of Sequences: 5004
Number of extensions: 32677
Number of successful extensions: 127
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 124
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 154067960
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -