Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= S06A01NCLL0008_K19
(687 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 134 1e-32
SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 133 3e-32
SPAC750.03c |||methyltransferase |Schizosaccharomyces pombe|chr ... 30 0.27
SPBC1348.04 |||methyltransferase |Schizosaccharomyces pombe|chr ... 30 0.27
SPBC32F12.06 |pch1||cyclin Pch1|Schizosaccharomyces pombe|chr 2|... 29 0.63
SPAC977.03 |||methyltransferase |Schizosaccharomyces pombe|chr 1... 29 0.83
SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 27 2.5
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 27 3.4
SPCC320.05 |||sulphate transporter |Schizosaccharomyces pombe|ch... 25 7.8
SPAC3F10.03 |||glycine tRNA-ligase|Schizosaccharomyces pombe|chr... 25 7.8
SPCC553.06 |||oligosaccharyltransferase subunit|Schizosaccharomy... 25 7.8
>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 134 bits (324), Expect = 1e-32
Identities = 76/181 (41%), Positives = 103/181 (56%), Gaps = 2/181 (1%)
Frame = +2
Query: 149 SLPLPFVFKAPIRPDLVM-MSTYQCRRTRATL-LRQQGGRSSTAPSLGXXXXXXXXXXXS 322
+L LPFVFKAPIRPDLV + T + R + ++ G ++A S G
Sbjct: 21 TLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAGHQTSAESWGTGRALARIPRVG 80
Query: 323 WWWYPQIWAGCIR*HVPRRTYVRAETKPWRRWHRRVNLRQRCXXXXXXXXXSGVPALVQA 502
+ ++ R + A TK WR+WH +VN ++ SGVP+L+ A
Sbjct: 81 GGGTHRSGQAAFG-NMCRSGRMFAPTKTWRKWHVKVNQNEKRYAIASAVAASGVPSLLLA 139
Query: 503 RGHIVEKIPELPLVVSDNVQEINKTKHAVIFLRRIKAWSDVLKVYKSQRLRAGKGKMRNR 682
RGH +E+IPE+PLVV D VQ KTK AV L+ IKA+ DV+KV S++LRAGKGK+RNR
Sbjct: 140 RGHRIEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVIKVANSRKLRAGKGKLRNR 199
Query: 683 R 685
R
Sbjct: 200 R 200
Score = 64.1 bits (149), Expect = 2e-11
Identities = 31/59 (52%), Positives = 36/59 (61%), Gaps = 1/59 (1%)
Frame = +3
Query: 222 EERAPPYCVSKEAGHQ-QRRVLGYRSCCSPYSPCRGGGTHRSGQGAFGNMCRGGRMFAP 395
+ + PY VS++AGHQ G + GGGTHRSGQ AFGNMCR GRMFAP
Sbjct: 46 KNKRQPYAVSEKAGHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAP 104
>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 133 bits (321), Expect = 3e-32
Identities = 75/181 (41%), Positives = 103/181 (56%), Gaps = 2/181 (1%)
Frame = +2
Query: 149 SLPLPFVFKAPIRPDLVM-MSTYQCRRTRATL-LRQQGGRSSTAPSLGXXXXXXXXXXXS 322
++ LPFVFKAPIRPDLV + T + R + ++ G ++A S G
Sbjct: 21 TIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAGHQTSAESWGTGRALARIPRVG 80
Query: 323 WWWYPQIWAGCIR*HVPRRTYVRAETKPWRRWHRRVNLRQRCXXXXXXXXXSGVPALVQA 502
+ ++ R + A TK WR+WH +VN ++ SGVP+L+ A
Sbjct: 81 GGGTHRSGQAAFG-NMCRSGRMFAPTKTWRKWHVKVNQNEKRYAISSAVAASGVPSLLLA 139
Query: 503 RGHIVEKIPELPLVVSDNVQEINKTKHAVIFLRRIKAWSDVLKVYKSQRLRAGKGKMRNR 682
RGH +E+IPE+PLVV D VQ KTK AV L+ IKA+ DV+KV S++LRAGKGK+RNR
Sbjct: 140 RGHRIEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVVKVANSRKLRAGKGKLRNR 199
Query: 683 R 685
R
Sbjct: 200 R 200
Score = 64.1 bits (149), Expect = 2e-11
Identities = 31/59 (52%), Positives = 36/59 (61%), Gaps = 1/59 (1%)
Frame = +3
Query: 222 EERAPPYCVSKEAGHQ-QRRVLGYRSCCSPYSPCRGGGTHRSGQGAFGNMCRGGRMFAP 395
+ + PY VS++AGHQ G + GGGTHRSGQ AFGNMCR GRMFAP
Sbjct: 46 KNKRQPYAVSEKAGHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAP 104
>SPAC750.03c |||methyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 145
Score = 30.3 bits (65), Expect = 0.27
Identities = 18/58 (31%), Positives = 24/58 (41%)
Frame = -3
Query: 211 RGHHDEVRSDGRLEYKREGERSPWTTHFSDTRHKVGYDRLISLYQRSYSSKKHYRRWQ 38
+GH D V G + P+T S H D + L S ++K YRRWQ
Sbjct: 72 KGHFDTVIDIGCFHSILNSDHEPYTASLSHICHS---DSSVFLRAFSETNKSRYRRWQ 126
>SPBC1348.04 |||methyltransferase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 145
Score = 30.3 bits (65), Expect = 0.27
Identities = 18/58 (31%), Positives = 24/58 (41%)
Frame = -3
Query: 211 RGHHDEVRSDGRLEYKREGERSPWTTHFSDTRHKVGYDRLISLYQRSYSSKKHYRRWQ 38
+GH D V G + P+T S H D + L S ++K YRRWQ
Sbjct: 72 KGHFDTVIDIGCFHSILNSDHEPYTASLSHICHS---DSSVFLRAFSETNKSRYRRWQ 126
>SPBC32F12.06 |pch1||cyclin Pch1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 342
Score = 29.1 bits (62), Expect = 0.63
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -1
Query: 126 PIHDIKSGMTDSFRFISEVIPPKNTID 46
P H SG TDSF ++ P K+T+D
Sbjct: 291 PQHQNSSGRTDSFHSLNTETPSKSTVD 317
>SPAC977.03 |||methyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 145
Score = 28.7 bits (61), Expect = 0.83
Identities = 18/58 (31%), Positives = 23/58 (39%)
Frame = -3
Query: 211 RGHHDEVRSDGRLEYKREGERSPWTTHFSDTRHKVGYDRLISLYQRSYSSKKHYRRWQ 38
+GH D V G + P T S H D + L S ++K YRRWQ
Sbjct: 72 KGHFDTVIDIGCFHSILNSDHEPHTASLSHICHS---DSSVFLRAFSETNKSRYRRWQ 126
>SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1462
Score = 27.1 bits (57), Expect = 2.5
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = -3
Query: 175 LEYKREGERSPWTTHFSDTRHKVGYDRLISL 83
+EYK + S + HF DTR K+ YD + L
Sbjct: 1242 VEYKVDSNES--SEHFQDTRWKIIYDHVCKL 1270
>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 500
Score = 26.6 bits (56), Expect = 3.4
Identities = 14/30 (46%), Positives = 16/30 (53%)
Frame = +3
Query: 291 RSCCSPYSPCRGGGTHRSGQGAFGNMCRGG 380
R C +S R GG R G+G FG RGG
Sbjct: 434 RPCRLDFSTPRTGGGSRGGRGGFGG--RGG 461
>SPCC320.05 |||sulphate transporter |Schizosaccharomyces pombe|chr
3|||Manual
Length = 667
Score = 25.4 bits (53), Expect = 7.8
Identities = 10/18 (55%), Positives = 15/18 (83%)
Frame = -2
Query: 290 VPQDSALLMTGLLADAVG 237
VPQ++A+L+TGL+A G
Sbjct: 156 VPQETAILVTGLIAFIAG 173
>SPAC3F10.03 |||glycine tRNA-ligase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 652
Score = 25.4 bits (53), Expect = 7.8
Identities = 22/61 (36%), Positives = 31/61 (50%), Gaps = 7/61 (11%)
Frame = +2
Query: 512 IVEKIPELPLVVSDNVQEINKTKHAVIFLRRIKA-------WSDVLKVYKSQRLRAGKGK 670
+ E +PE P+VV E+N+ K F R KA W + KV KS +L A +GK
Sbjct: 405 VQEALPE-PVVVEQFEVEVNRKKFGPRFKRDAKAVEEAMISWPESEKVEKSAQLVA-EGK 462
Query: 671 M 673
+
Sbjct: 463 I 463
>SPCC553.06 |||oligosaccharyltransferase subunit|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 271
Score = 25.4 bits (53), Expect = 7.8
Identities = 14/48 (29%), Positives = 19/48 (39%)
Frame = -3
Query: 445 SLP*VHATVPAPPRLGFGANIRPPRHMLPNAPCPDLWVPPPRHGEYGL 302
SLP ++ PP L A P + P + PPP+ Y L
Sbjct: 142 SLPLGQLSINVPPSLYHKAEFSPLDELSPKEVILHTFSPPPKRANYFL 189
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,001,794
Number of Sequences: 5004
Number of extensions: 63494
Number of successful extensions: 181
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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